RESUMO
A novel polyisoprenylated benzophenone (1) has been isolated from an ethanol extract of Cuban propolis. Its structure has been determined using high-field 2D NMR techniques. Compound 1 showed significant antimicrobial and antifungal activity against a variety of bacteria and yeasts.
Assuntos
Anti-Infecciosos/isolamento & purificação , Antifúngicos/isolamento & purificação , Benzofenonas/isolamento & purificação , Própole/química , Antibacterianos , Anti-Infecciosos/química , Antifúngicos/química , Bactérias/efeitos dos fármacos , Benzofenonas/química , Cromatografia Líquida de Alta Pressão , Cuba , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Espectrofotometria Infravermelho , Leveduras/efeitos dos fármacosRESUMO
A study on the antibacterial properties of alkaloidal fractions of Vallesia antillana Wood, a Cuban plant belonging to the Apocynaceae family, is performed for the first time. Four partial fractions from bark of stems were prepared according to a classical extraction of total alkaloids and a partial fractionation of these, with the use of tartaric acid and ethyl ether and by a pH gradient with values of 2.5, 5.0, 7.0 and alkaline one. Data from thin layer chromatography on the alkaloidal content of different fractions are added and an assessment of antibacterial activity was made; for this, a method of sections in agar medium with bacterial species of human clinical interest was used; inhibitory halos found within 24 hours of incubation at 37 degrees C are reported in millimeters.
Assuntos
Alcaloides/análise , Cromatografia em Camada Fina/métodos , Extratos Vegetais/análise , Plantas Tóxicas/análise , Alcaloides/farmacologia , Antibacterianos , Bactérias/efeitos dos fármacos , Avaliação de MedicamentosRESUMO
Bacteriostatic properties of vindolinina (an alkaloid isolated from Catharanthus roseus that has an ester group within its molecule) as well as of its alkaline-hydrolysis product (vindolininic acid) crystallized as chlorhydrate, and of the product of its reduction with lithium and aluminum hydrides (vindolininol) are compared. Several strains of bacteria pathogenic to man (Proteus, Escherichia, Shigella, Staphylococcus, and Pseudomonas) cultured in nutritive-agar dishes containing disks and incubated at 37 degrees C were used and results were assessed 24 hours later. Data obtained show that bacterial growth inhibition is closely related to the structure of the compound as well as to its type of grouping.