RESUMO
Background: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA. Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.
Assuntos
Bioensaio/métodos , Sangue/parasitologia , Cisticercose/sangue , Cisticercose/diagnóstico , Cysticercus/isolamento & purificação , Animais , Bovinos , Ácido Desoxicólico , Ditiotreitol , Equador/epidemiologia , Reações Falso-Positivas , Feminino , Humanos , MasculinoRESUMO
Se presenta un caso inusual de infección por Hymenolepis diminuta en infante de un año de edad residente en Maracay, estado Aragua. El hallazgo se realizó de manera fortuita por el personal del Laboratorio de Parasitosis Intestinales y Serología de Esquistosomiasis de la Dirección General de Salud Ambiental del Ministerio del Poder Popular para la Salud, a donde fueron remitidos ejemplares adultos del parásito eliminado de forma espontánea por el paciente. Antes de la expulsión del parásito, el paciente presentó sintomatología digestiva, fiebre y manifestaciones alérgicas. La representante del infante refirió la presencia de ratas y ratones en el jardín de la vivienda. Este caso se constituye en el primer informe de infección por H. diminuta en el estado Aragua. Se sugiere realizar investigación epidemiológica más rigurosa para determinar la prevalencia de himenolepiosis.
Presentation of an unusual case of Hymenolepis diminuta infection in a one-year old infant residing at Maracay, Aragua State. The finding occurred fortuitously by the staff of the Intestinal Parasites and Schistosomiasis Serology Laboratory of the General Direction of Environmental Health of the Ministerio del Poder Popular para La Salud, who received two adult forms of the parasite eliminated spontaneously by the patient. Before the expulsion of the parasite, the patient had digestive symptoms, fever and allergic reactions. The infants guardian referred the presence of rats and mice in the garden of the house. This case is the first report of this type of infection by H. diminuta in Aragua State. A more rigorous epidemiological investigation is suggested to determine the prevalence of himenolepiosis.
RESUMO
To study diagnostic epitopes within the Taenia solium 8 kDa antigen family, six overlapping synthetic peptides from an 8 kDa family member (Ts8B2) were synthesized and evaluated by ELISA and MABA with sera from patients with neurocysticercosis (NCC), from infected pigs and from rabbits immunized with recombinant Ts8B2 protein. The pre-immune rabbit sera and the Ts8B2 recombinant protein served as negative and positive controls, respectively. A similar analysis was done with the already described antigenic peptides from another member of the 8 kDa family, highly similar to Ts8B2, the CyDA antigen. Surprisingly, neither the Ts8B2 peptides nor the CyDA peptides were recognized by infected human and porcine sera. However, the entire Ts8B2 recombinant, as well as amino and carboxy-terminal halves were recognized by the positive serum samples. The observed lack of recognition of linear Ts8B2 peptides suggests that the principal serological response to the Ts8B2 family is focused on conformational epitopes in contrast to the previously observed antigenicity of the CyDA peptides. This differential antigenicity of 8 kDa family peptides could be related with parasite antigenic variability. The fact that rabbits experimentally immunized with Ts8B2 did make anti-peptide antibodies to peptides Ts8B2-6 and CyDA-6, located in the carboxy-terminal region demonstrated that the Ts8B2 peptides are not intrinsically non-immunogenic.
Assuntos
Antígenos de Helmintos/imunologia , Cisticercose/diagnóstico , Epitopos/imunologia , Taenia solium/imunologia , Sequência de Aminoácidos , Animais , Variação Antigênica , Antígenos de Helmintos/química , Antígenos de Helmintos/genética , Clonagem Molecular , Cisticercose/imunologia , Cisticercose/parasitologia , Cysticercus/genética , Cysticercus/imunologia , Cysticercus/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Epitopos/química , Epitopos/genética , Regulação da Expressão Gênica , Humanos , Immunoblotting , Coelhos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Sensibilidade e Especificidade , Alinhamento de Sequência , Suínos , Taenia solium/genética , Taenia solium/isolamento & purificaçãoRESUMO
La evaluación del estado nutricional, especialmente en grupos vulnerables, permite medir en forma indirecta la calidad de vida de una comunidad. Objetivo: Conocer el estado nutricional de niños que viven una comunidad rural del Estado Cojedes y las condiciones socioeconómicas de su grupo familiar. Metodología: Estudio descriptivo, transversal con 31 niños de 6 a 14 años, de ambos sexos. El diagnóstico nutricional se realizó por los indicadores: Talla-edad. Peso-talla, Peso-edad e Índice de Masa Corporal (IMC). Se utilizó para la clasificación, la referencia nacional y para la estratificación socioeconómica, Graffar - Méndez Castellano. En el análisis estadístico se utilizó distribución de frecuencia con porcentajes y medidas de tendencia central para las diferentes variables. Resultados: 83,9 % de los niños tuvo diagnóstico nutricional normal y 28,1 % presentó talla baja, 83,3% de las familias se encontró en situación de pobreza crítica. Conclusión: pese al estado de pobreza de las familias evaluadas, la mayoría de los escolares tienen IMC normal. Sin embargo, el hallazgo de talla baja refleja los posibles efectos deletéreos de la condición socioeconómica que predomina en la comunidad.
The evaluation of the nutritional state, especially in vulnerable groups, allows to measure in indirect form the quality of life of a community. Objetive: to know the state nutritional children who live a rural community on the Cojedes State and the socioeconomics conditions of their familiar group. Methodology: A descriptive, cross-sectional study was made where 31 children of 6 to 14 years, both sexes evaluated themselves. The nutritional diagnosis was made by the indicators: Stature-age. Weigh-it stature, Weigh-age and Index of Corporal Mass (IMC). Classification was used the national reference, and for the socioeconomic stratification, Graffar - Méndez Castellanos. For the statistical analysis the frequency allocation in percentage and measures of central tendency for the different variables was used. Results: 83,9% of the children had I diagnose normal nutritional and 28,1 % presented/displayed low stature. 83,3% of the families were in situation of critical poverty. Conclusion: in spite of the state of poverty of the evaluated families, most of the students they have a normal nutritional diagnosis, but the present low stature, reflects the possible deleterious effects of the socioeconomic condition that predominates.