RESUMO
The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of blood cells in the hemogram and IgA and IgG production. MOS was effective in recovering of EPEC-infected dogs since prebiotic-treated animals recovered more rapidly from infection than untreated ones (p < 0.05). This is an important finding since diarrhea causes intense dehydration and nutrient loss. The use of prebiotics for humans and other animals with diarrhea can be an alternative for the treatment and prophylaxis of EPEC infections.
Assuntos
Sangue/imunologia , Diarreia/microbiologia , Escherichia coli Enteropatogênica/imunologia , Fezes , Fármacos Gastrointestinais/metabolismo , Oligossacarídeos/metabolismo , Prebióticos , Animais , Anticorpos Antibacterianos/sangue , Fenômenos Químicos , Modelos Animais de Doenças , Cães , Escherichia coli , Fármacos Gastrointestinais/administração & dosagem , Fármacos Gastrointestinais/química , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Leucócitos/imunologia , Oligossacarídeos/administração & dosagem , Oligossacarídeos/químicaRESUMO
The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of blood cells in the hemogram and IgA and IgG production. MOS was effective in recovering of EPEC-infected dogs since prebiotic-treated animals recovered more rapidly from infection than untreated ones (p < 0.05). This is an important finding since diarrhea causes intense dehydration and nutrient loss. The use of prebiotics for humans and other animals with diarrhea can be an alternative for the treatment and prophylaxis of EPEC infections.
Assuntos
Animais , Cães , Sangue/imunologia , Diarreia/microbiologia , Escherichia coli Enteropatogênica/imunologia , Fezes , Fármacos Gastrointestinais/metabolismo , Oligossacarídeos/metabolismo , Prebióticos , Anticorpos Antibacterianos/sangue , Fenômenos Químicos , Modelos Animais de Doenças , Escherichia coli , Fármacos Gastrointestinais/administração & dosagem , Fármacos Gastrointestinais/química , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Leucócitos/imunologia , Oligossacarídeos/administração & dosagem , Oligossacarídeos/químicaRESUMO
The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of blood cells in the hemogram and IgA and IgG production. MOS was effective in recovering of EPEC-infected dogs since prebiotic-treated animals recovered more rapidly from infection than untreated ones (p < 0.05). This is an important finding since diarrhea causes intense dehydration and nutrient loss. The use of prebiotics for humans and other animals with diarrhea can be an alternative for the treatment and prophylaxis of EPEC infections.(AU)
Assuntos
Cães , Bioensaio , Imunoglobulinas , Reação em Cadeia da Polimerase , Escherichia coliRESUMO
This paper discusses the first analytical determination of the widely used fungicide thiabendazole by nylon-induced phosphorimetry. Nylon was investigated as a novel solid-matrix for inducing room-temperature phosphorescence of thiabendazole, which was enhanced under the effect of external heavy-atom salts. Among the investigated salts, lead(II) acetate was the most effective in yielding a high phosphorescence signal. An additional enhancement of the phosphorescence emission was attained when the measurements were carried out under a nitrogen atmosphere. There was only a moderate increase in the presence of cyclodextrins. The room-temperature phosphorescence lifetimes of the adsorbed thiabendazole were measured under different working conditions and, in all cases, two decaying components were detected. On the basis of the obtained results, a very simple and sensitive phosphorimetric method for the determination of thiabendazole was established. The analytical figures of merit obtained under the best experimental conditions were: linear calibration range from 0.031 to 0.26 microg ml(-1) (the lowest value corresponds to the quantitation limit), relative standard deviation, 2.4% (n=5) at a level of 0.096 microg ml(-1), and limit of detection calculated according to 1995 IUPAC Recommendations equal to 0.010 microg ml(-1) (0.03 ng/spot). The potential interference from common agrochemicals was also studied. The feasibility of determining thiabendazole in real samples was successfully evaluated through the analysis of spiked river, tap and mineral water samples.
RESUMO
Utilizando o material clinico de duas sessoes de um paciente em psicanalise, o autor e tentado a analisar algumas correlacoes entre o sintoma apresentado - labirintite - e uma lenda mitologica. Primeiramente, na introducao, o autor discute quatro pontos sobre mitos a respeito dos quais persistem algumas duvidas, ou sejam" a) a validade de usar mitos como modelos nas pesquisas psicanalitica, sociologica e antropologica; b)se o conteudo dos mitos e realmente uma expressao livre e direta do inconsciente; c)a diferenca entre mitos e contos; d) a universalidade da linguagem mitologica. Finalmente, o autor discute o material clinico e a condensacao expressa na figura do minotauro