RESUMO
The effect of oestradiol on the intact and castrated adult gerbil prostate was evaluated by focussing on stromal and epithelial disorders, and hormonal receptor immunoreactivity. The experimental animals were studied by histological, histochemical and immunohistochemical techniques, morphometric-stereological analysis and transmission electron microscopy. Epithelial alterations in the oestradiol-treated animals were frequent, with an increase in epithelial cell height, areas of intense dysplasia and hyperplasia and formation of prostatic intraepithelial neoplasia (PIN). Another aspect that did not depend on the presence of testosterone was the arrangement of the fibrillar and non-fibrillar elements of the extracellular matrix among smooth muscle cells (SMC), suggesting a possible role of these cells in rearrangement and synthesis of these components, after oestrogenic treatment. In the castrated animals, an accumulation of extracellular matrix elements under the epithelium was evident, while in the intact animals the same compounds were dispersed and scarce. In the groups of intact and castrated animals, SMC and fibroblasts exhibited a secretory phenotype, which was accentuated after oestradiol administration. There was an increase of the immunoreactivity to alpha-oestrogen and androgen receptors in hyperplastic areas compared to normal epithelium, revealing the involvement of these steroid receptors in the hyperplasia and PIN development.
Assuntos
Células Epiteliais/efeitos dos fármacos , Estrogênios/farmacologia , Próstata/efeitos dos fármacos , Células Estromais/efeitos dos fármacos , Análise de Variância , Animais , Células Epiteliais/ultraestrutura , Epitélio/efeitos dos fármacos , Gerbillinae , Masculino , Microscopia Eletrônica de Transmissão/métodos , Orquiectomia , Próstata/ultraestrutura , Células Estromais/ultraestruturaRESUMO
The incidence of ciliated cells in the prostate gland of the female gerbil (Meriones unguiculatus) is uncommon and apparently becomes more frequent during androgen (testosterone cypionate) and anti-estrogen (letrozole) endocrine therapies. To evaluate the effects of such drug therapies on the induction of ciliogenesis in the glandular epithelium of female prostate glands, adult female gerbils aged 90 days were treated for 14 days with testosterone and letrozole after which their prostate glands were removed for histological, ultrastructural, and serological analyses. The cytodifferentiation of the ciliated phenotype in the alveolar epithelium became more frequent after both the testosterone and the letrozole treatments. The ciliogenesis phenomenon of the epithelial cells in the prostate gland of female gerbils thus appears to be induced by variations in the increase of androgen levels.
Assuntos
Diferenciação Celular/efeitos dos fármacos , Cílios/fisiologia , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Terapia de Reposição Hormonal , Próstata/efeitos dos fármacos , Animais , Peso Corporal , Feminino , Gerbillinae , Hormônios Esteroides Gonadais/sangue , Terapia de Reposição Hormonal/efeitos adversos , Letrozol , Masculino , Nitrilas/efeitos adversos , Nitrilas/farmacologia , Tamanho do Órgão , Próstata/anatomia & histologia , Próstata/citologia , Testosterona/efeitos adversos , Testosterona/farmacologia , Triazóis/efeitos adversos , Triazóis/farmacologiaRESUMO
Mongolian gerbils (Meriones unguiculatus) were grouped into two experimental groups: GEx.01 suffered orchiectomy and after 30 days received doses of testosterone cipionate (T), while GEx.02 received weekly and alternated doses of the anti-androgens cyproterone acetate and flutamide for 30 days, and the animals were then euthanized. Structural evaluation reveals a more intense reduction in epithelial height in GEx.02. Smooth muscle cells (SMC) presented a star-shaped aspect after 30 days of hormonal ablation and basal membrane was shown to be more intensely grooved in GEx.01. In both groups, after hormonal replacement, recovery in epithelial height could be noted and the SMC presented its phenotypes, but an increase in RER was seen, characterizing a modulation from its contractile to secreting phenotype. In conclusion, the prostate presented involution capacity after androgen ablation and the ability to reorganize after hormonal replacement, but events resulting from orchiectomy and subsequent T replacement were shown to be more aggressive to the prostate.
Assuntos
Antagonistas de Androgênios/administração & dosagem , Orquiectomia , Próstata/patologia , Hiperplasia Prostática/patologia , Testosterona/administração & dosagem , Fosfatase Ácida , Animais , Ciproterona , Modelos Animais de Doenças , Retículo Endoplasmático Rugoso/ultraestrutura , Endotélio/ultraestrutura , Flutamida , Gerbillinae , Imuno-Histoquímica , Masculino , Microscopia Eletrônica de Transmissão , Orquiectomia/métodos , Tamanho do Órgão/efeitos dos fármacos , Próstata/efeitos dos fármacos , Próstata/crescimento & desenvolvimento , Próstata/metabolismo , Hiperplasia Prostática/induzido quimicamente , Hiperplasia Prostática/metabolismo , Proteínas Tirosina Fosfatases/metabolismo , Receptores Androgênicos/efeitos dos fármacos , Receptores Androgênicos/metabolismo , Testosterona/sangueRESUMO
The elastic system fibers are abundant elements of the extracellular matrix found in organs such as skin, blood vessels, lung and in elastic cartilage and elastic tendons. These fibers have been studied by several selective staining methods, such as resorcin-fuchsin for light microscopy and hematoxylin-eosin plus fluorescence and confocal scanning laser microscopy. However, most of these techniques are only efficient for tissues embedded in paraffin or paraplast, since most dyes show low penetration into glycol methacrylate resins. In this report, we describe a variation of Weigert´s resorcin-fuchsin method that involves the pretreatment of resin sections with potassium permanganate. This procedure increased the affinity between the dye and elastic fibers, and stained the elastic fibers in black of dark violet, the nuclei in purple and other structures in light blue. Thus, this modification of the original method provided excellent artifact-free demarcation of elastic fibers in well-preserved tissues.