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The effect of different feed substrates incubated in filter bags [no bag (NB); Ankom® F57, 25 µm pore size (25AN); polyester, 45 µm pore size (45PB); polyester, 67 µm pore size (67PB) ] or dispersed in the medium on gas production, digestion and rumen fermentation was evaluated using an in vitro gas production system. Filter bags reduced (P < 0.01) gas production but increased (P < 0.01) the in vitro dry matter digestibility (45PB and 67PB). Additionally, the use of filter bags with smaller pore size, reduced total volatile fatty acid (P < 0.01), but had minimal effects on acetate, propionate, and butyrate concentration. Our research suggested that using filter bags with a pore size of 67 µ would reduce some negative effects of incubating feed substrate dispersed in the medium or in filter bags with smaller pore size. However, the use of bags with a larger pore size might allow the wash out of small feed particles with consequent overestimation of digestibility.

O efeito de diferentes substratos alimentares incubados em sacos de filtro [sem saco (NB); Ankom® F57, tamanho de poro de 25 µm (25AN); poliéster, tamanho de poro de 45 µm (45PB); poliéster, tamanho de poro de 67 µm (67PB) ] ou disperso no meio na produção de gás, digestão e fermentação ruminal foi avaliada usando um sistema de produção de gás in vitro. As bolsas de filtro reduziram (P < 0,01) a produção de gases, mas aumentaram (P < 0,01) a digestibilidade da matéria seca in vitro (45PB e 67PB). Adicionalmente, o uso de bolsas de filtro com tamanho de poro menor reduziu o ácido graxo volátil total (P < 0,01), mas teve efeitos mínimos na concentração de acetato, propionato e butirato. Nosso trabalho sugere que usar sacos de filtro com tamanho de poro de 67 µ reduziria alguns efeitos negativos da incubação de substrato de alimentação disperso no meio ou em sacos de filtro com tamanho de poro menor. No entanto, o uso de sacolas com poros maiores pode permitir a eliminação de pequenas partículas de ração com consequente superestimação da digestibilidade.

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Several classical problems in symbolic dynamics concern the characterization of the simplex of measures of maximal entropy. For subshifts of finite type in higher dimensions, methods of statistical mechanics are ideal for dealing with these problems. R. Burton and J. Steif developed a strategy to construct examples of strongly irreducible subshifts of finite type admitting several measures of maximal entropy. This strategy exploits a correspondence between equilibrium statistical mechanics and symbolic dynamics-a correspondence which was later formalized by O. Häggström. In this paper, we revisit and discuss this correspondence with the aim of presenting a simplified version of it and present some applications of rigorous results concerning the Potts model and the six-vertex model to symbolic dynamics, illustrating in this way the possibilities of this correspondence.

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Chaya and amaranth are Mexican traditional foods with a high nutritional value. Many studies have demonstrated the individual beneficial effect of each. However, there is no evidence of the use of these foods on the formulation of functional foods. This study evaluated the effect of replacing 5-20% of wheat flour with chaya and amaranth flours to generate four different formulations of cookies. Proximal analysis, total polyphenols and oxalate content, antioxidant activity, fatty acid profile, and sensory analysis were performed on the cookies. The results of the chemical composition showed that all cookies have a high protein content (9.21-10.10%), an adequate amount of fiber (5.34-6.63%), and a balanced ratio of unsaturated-saturated fatty acids (70:20), and they contain PUFAs (50.4-53.2 g/100 g of fatty acids), especially α-linolenic and oleic acids. All formulations presented antioxidant activity (2540 ± 65.9 to 4867 ± 61.7 Trolox equivalents (µmoles/100 g)) and polyphenols (328-790 mg/100 g); in particular, quercetin was identified in their composition. Results of the sensory analysis indicated that incorporation of chaya and amaranth flour in cookies does not affect the acceptability of the products. The inclusion of traditional foods, such as chaya and amaranth, in cookies enhances their nutritional value and increases the content of bioactive compounds associated with health effects.

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The objective of this study was to determine milk fatty acids from crossbred F1 dairy cows fed on tropical grasses and supplemented with different levels of concentrate. Twelve dairy cows (50% Holstein × 50% Brahman) with 60 days of lactation grazing tropical grasses were assigned to a Switchback design, with three periods of 15 days with different concentrate levels: 0, 150, 300 and 450 g /kg. Milk samples were obtained on the last five days of each experimental period. Milk yield and milk composition were not affected. Cows fed with 300 g/kg of concentrate had higher contents of C15:0 (p = 0.004), C22:0 (p = 0.031), and C24:0 (p = 0.013). C17:1 cis9 was higher (p = 0.039) with 150 g/kg and lowest with 450 g/kg. C18:1 cis9 was higher (p = 0.042) with 150 g/kg. C18:2n6trans was higher (p = 0.05) with 300 g/kg and lower (p = 0.018) with 450 g/kg. This study shows that adding up to 450 g/kg of concentrate to crossbred F1 dairy cows fed on tropical grasses does not have negative effects on milk yield and milk quality. Therefore, under these production conditions, farmers can rely on tropical grasses and reduce feeding costs.

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The objective of this study was to evaluate in vitro rumen fermentation and methane production under the influence of two sources of phytochemicals: essential oils (EOs) and aqueous extracts (AEs). Treatments were set up in a completely randomized block design, with 4×2+1 factorial arrangement of four species, S (garlic, G; cinnamon, C; rosemary, R; eucalyptus; EU) × two types of presentation, P (essential oil, EO; aqueous extract, AE) and a basal diet, BD (50% concentrate, 20% alfalfa and 30% corn silage). Rumen fermentation was evaluated using the in vitro gas production technique. All experimental units were incubated with 500 mg of BD for 72 hours. Treatments were added at a single dose of 900 mg/L of rumen inoculum. Gas pressure was recorded at 0, 2, 4, 6, 8, 10, 14, 18, 24, 30, 36, 42, 48, 60 and 72 h postincubation. There was an interaction effect (P × S) between plant extract presentation (P) and plant species (S) for all variables. Treatments GEO, CEO, REO decreased volatile fatty acids (mmol/200 mg), microbial mass production (mg/g), CH4 production (mL/g), in vitro dry matter digestibility (P < 0.05), and total gas production at 24 and 72 h post-incubation (P < 0.05; mL/g DM, mL/g OM). No differences (P > 0.05) were observed between AEs and BD. In conclusion, the use of EOs negatively affected rumen fermentation parameters and the production of CH4. Garlic and cinnamon EOs effectively reduced methane emissions; however, they also reduced in vitro dry matter digestibility.

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Two experiments were conducted to compare a supplemental blend of essential oils alone (EO) or combined with enzymes (EO + ENZ) versus virginiamycin (VM), on characteristics of growth performance (Exp. 1) and digestion (Exp. 2) in finishing lambs. Lambs were fed a high-energy finishing diet supplemented with: (1) no supplement (control); (2) 150 mg supplemental EO; (3) 150 mg supplemental EO plus 560 mg alpha-amylase (EO + ENZ); and 4) 25 mg VM. Compared with the control, growth performance response to EO and VM were similar, enhancing (5.7%, p < 0.05) feed efficiency and observed dietary net energy. Compared with control, supplementation with EO + ENZ tended (p = 0.09) to increase dry matter intake (6.8%), improving (p < 0.05) weight gain and feed efficiency (10.4 and 4.4%, respectively). Dietary energy utilization was greater (2.7%, p < 0.05) for EO and VM than EO + ENZ. Treatment effects on the carcass and visceral mass were small, but additive supplementation decreased (p ≤ 0.03) the relative weight of the intestines. There were no treatment effects on measures of digestion nor digestible energy of the diet. Supplemental EO may be an effective alternative to VM in high-energy finishing diets for feedlot lambs. Combination EO + ENZ may further enhance dry matter intake, promoting increased weight gain.

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The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.(AU)

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The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.(AU)

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The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus – ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.

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The current pandemic is caused by the coronavirus disease 2019 (COVID-19), which is, in turn, induced by a novel coronavirus (SARS-CoV-2) that triggers an acute respiratory disease. In recent years, the emergence of SARS-CoV-2 is the third highly pathogenic event and large-scale epidemic affecting the human population. It follows the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003 and the Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012. This novel SARS-CoV-2 employs the angiotensin-converting enzyme 2 (ACE2) receptor, like SARS-CoV, and spreads principally in the respiratory tract. The viral spike (S) protein of coronaviruses facilities the attachment to the cellular receptor, entrance, and membrane fusion. The S protein is a glycoprotein and is critical to elicit an immune response. Glycosylation is a biologically significant post-translational modification in virus surface proteins. These glycans play important roles in the viral life cycle, structure, immune evasion, and cell infection. However, it is necessary to search for new information about viral behavior and immunological host's response after SARS-CoV-2 infection. The present review discusses the implications of the CoV-2 S protein glycosylation in the SARS-CoV-2/ACE2 interaction and the immunological response. Elucidation of the glycan repertoire on the spike protein can propel research for the development of an appropriate vaccine.

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INTRODUCTION: Autoimmune thyroid disease (AITD) is the most common autoimmune disorder worldwide. Remarkably, it is commonly accompanied by other autoimmune diseases, such as rheumatoid arthritis (RA). The immunopathogenic mechanisms behind the coexistence of these disorders are still not completely understood. Immunogenetics influences the physiopathology of these diseases since ethnicity plays an essential role in the inheritance of susceptibility markers. METHODS: High-resolution HLA class II typing was performed using a sequence-based method. RESULTS: The allele frequency of HLA-DRB1∗04:04 and -DRB1∗03:01 were significantly increased in patients with AITD and RA compared to healthy individuals, pC â€‹= â€‹0.021, OR â€‹= â€‹2.4, 95%CI â€‹= â€‹1.19-4.75 and pC â€‹= â€‹0.009, OR â€‹= â€‹3.4, 95%CI â€‹= â€‹1.42-7.93, respectively. Remarkably, these patients have a combined risk given by susceptibility HLA-DRB1 alleles that contain the shared epitope, pC â€‹= â€‹0.03, OR â€‹= â€‹1.7, IC95% â€‹= â€‹1.07-2.76, and a lack of protective alleles carrying aspartic acid70, pC â€‹= â€‹0.009, OR â€‹= â€‹0.5, IC95% â€‹= â€‹0.32-0.84. DISCUSSION: The results suggest that patients with AITD and RA have an immunogenetic mechanism that combines the susceptibility alleles associated with both diseases. Importantly, it seems to be linked mainly to the lack of protective alleles with aspartic acid in the position 70, along with the presence of susceptibility alleles that have the sequences QRRAA, QKRAA, and RRRAA at positions 70-74. CONCLUSION: Patients with AITD and RA have a characteristic immunogenetic signature, which could be useful for determining multiple autoimmunities and assessing their relatives' risk of developing it.

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In this article, we report the case of a 43-year-old male patient with mononeuritis multiplex associated with antiphospholipid antibodies. The patient had no other clinical or laboratory features of other systemic or autoimmune diseases or systemic vasculitis. He was treated with oral anticoagulant and mycophenolate mofetil, and is progressing favorably.

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BACKGROUND/OBJECTIVE: The aim of this cross-sectional study was to explore which factors affect the impact of musculoskeletal ultrasound (MUS) on the treatment proposal among rheumatologists with different degree of experience. METHODS: Sixteen clinical vignettes summarized data from rheumatoid arthritis (RA) outpatients; vignettes included clinical evaluation and a blank section for a first treatment proposal; MUS information was then added, based on German Ultrasound score, followed by a blank section for treatment re-consideration, if applicable. During a 6 months period, each vignette was concomitantly presented to six trainees and six senior rheumatologists (SR); three SR had ≥15 years of experience. Participants were blinded to colleagues' responses. Appropriated statistics were used. RESULTS: Vignettes included data from female patients, who had a mean ± SD age of 43.3 ± 9 years, 7.6 ± 3.5 years of disease duration and comorbidities (68.8%). MUS induced treatment modification in 24% of evaluations, with similar percentage among SR and trainees. Within SR, more experienced rheumatologists (≥15 years) never translated MUS findings in a different treatment proposal, compared to 34% of those with lesser experience, p ≤ 0.0001. There were 60 clinical scenarios each, with remission and moderate disease activity, and 36 clinical scenarios each, with low and high disease activity. MUS-induced treatment modifications were more frequent in scenarios with low and moderate disease activity, compared to remission and high disease activity, p = 0.008. CONCLUSIONS: Physician's experience and disease activity level affect the impact of MUS on the treatment decision in RA outpatients. RA patients with intermediate disease activity may benefit from MUS incorporation to standard assessments.

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The partial substitution of soybean meal by Gliricidia sepium or Guazuma ulmifolia leaves in the rations of growing lambs was evaluated at an experimental station in the dry tropics of Mexico. Sixteen weaned crossbred male Pelibuey × Blackbelly lambs (initial weight 19 ± 1.66 kg), distributed in a completely randomized design, were assigned to the following protein sources: (a) 100% soybean meal, (b) 50% soybean meal + 50% G. sepium, (c) 50 % soybean meal + 25% G. ulmifolia + 25% G. sepium, and (d) 50% soybean meal + 50% G. ulmifolia in isoproteic rations. The lambs were housed in individual pens and fed ad libitum. Substitution of protein from soybean meal with G. ulmifolia did not affect gain, intake, or feed conversion; however, the inclusion of G. sepium reduced (linear effect, P < 0.01) gain and intake and impaired feed conversion (linear effect, P < 0.01). Partial substitution up to 50% of soybean meal with 50% G. ulmifolia leaves resulted in similar growth and lamb performance, but inclusion of G. sepium leaves adversely affected lamb growth, presumably due to other factors not related to the protein content.

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La toxocariasis sistémica generalmente se manifiesta conf iebre, hepatomegalya y adenomegalias. Reportamos una presentación poco usual que inicialmente fue confundida con una artritis reumatoide juvenil por haber fiebre, artralgia, artritis de pequeñas articulacioes, leucocitosis importante e hipergamaglobulinemia, además de factor reumatoide tipo IgM positivo. Después de la aparición en sangre y en médula ósea de una eosinofilia marcada, se diagnosticó toxocariasis sistémica al obtener títulos muy elevados de anticuerpos contra Toxocara canis por la técnica de ELISA. Se discuten brevemente los principales datos clínicos, de laboratorio y de manejo de la toxocariasis sistémica y el posible origen del factor reumatoide en esta enfermedad.

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