RESUMO
Squirrel monkeys, colony-born from Bolivian parents, were studied to establish the sequences and timing of eruption for deciduous and permanent teeth. Infants were born with a naked gingiva, and in only one monkey was di1 present at birth. The eruption of the lower deciduous teeth preceded their upper counterparts with the exception of di2, dc, and dpm2. No significant differences were found between the right and left quadrants of the mandible and maxilla. No significant sexual differences were found in the age of eruption. By the age of 14 weeks, all deciduous teeth had erupted. The sequence of eruption of the replacement teeth was different from that of milk teeth. The differences lie in the delayed eruption of canine teeth and in the inverted sequence, from the back to the front, of the premolar series. Significant sexual differences were found in total eruption (TE) for PM3 and I2 (P < 0.05) and highly significant differences (P < 0.01) in TE and initial eruption (IE) for C1, females being more precocious than males. The age at which monkeys completed dental eruption was highly variable, 103-119 weeks for males and 89-112 weeks for females. Differences were found when our results were compared with those of Long and Cooper [1968] for Colombian squirrel monkeys.
RESUMO
Owl monkeys (Aotus trivirgatus) were inoculated with XJ, a pathogenic strain of Junin virus, seeking new animal models for Argentine Hemorrhagic Fever. Nine monkeys were inoculated intramuscularly with 30 or 300,000 TCID50 of junin virus. Hematological and virological studies showed no alteration in blood elements such as red cell, reticular cell and platelets, up to 28 days after inoculation. Hemoglobin and hematocrit determinations also remained constant. However, significant neutropenia was seen at day 11 and minimal viremia was detected in some animals during the second and third week post-inoculation. No clinical or behavioral modifications were observed during the eighty-days observation period. Non-specific necropsy findings included pyelonephritis, pneumonitis, liver abscess and eosinophilic spleen infiltrate. All of these findings seem to be unrelated to Junin virus inoculation. No virus was present in organs of animals killed 29, 57 or 85 days post-inoculation. All nine owl monkeys developed serum neutralizing antibodies by day 22. It is concluded that the owl monkey suffers a subclinical infection when inoculated with Junin virus, similar to that seen in other primate species (Saimiri sciureus and Alouatta caraya).
Assuntos
Aotus trivirgatus , Cebidae , Febre Hemorrágica Americana/fisiopatologia , Animais , Anticorpos Antivirais/biossíntese , Arenavirus do Novo Mundo/imunologia , Suscetibilidade a Doenças , Febre Hemorrágica Americana/imunologia , Febre Hemorrágica Americana/patologia , Masculino , Neutropenia/etiologia , Viremia/etiologiaRESUMO
Owl monkeys (Aotus trivirgatus) were inoculated with XJ, a pathogenic strain of Junin virus, seeking new animal models for Argentine Hemorrhagic Fever. Nine monkeys were inoculated intramuscularly with 30 or 300,000 TCID50 of junin virus. Hematological and virological studies showed no alteration in blood elements such as red cell, reticular cell and platelets, up to 28 days after inoculation. Hemoglobin and hematocrit determinations also remained constant. However, significant neutropenia was seen at day 11 and minimal viremia was detected in some animals during the second and third week post-inoculation. No clinical or behavioral modifications were observed during the eighty-days observation period. Non-specific necropsy findings included pyelonephritis, pneumonitis, liver abscess and eosinophilic spleen infiltrate. All of these findings seem to be unrelated to Junin virus inoculation. No virus was present in organs of animals killed 29, 57 or 85 days post-inoculation. All nine owl monkeys developed serum neutralizing antibodies by day 22. It is concluded that the owl monkey suffers a subclinical infection when inoculated with Junin virus, similar to that seen in other primate species (Saimiri sciureus and Alouatta caraya).
RESUMO
Owl monkeys (Aotus trivirgatus) were inoculated with XJ, a pathogenic strain of Junin virus, seeking new animal models for Argentine Hemorrhagic Fever. Nine monkeys were inoculated intramuscularly with 30 or 300,000 TCID50 of junin virus. Hematological and virological studies showed no alteration in blood elements such as red cell, reticular cell and platelets, up to 28 days after inoculation. Hemoglobin and hematocrit determinations also remained constant. However, significant neutropenia was seen at day 11 and minimal viremia was detected in some animals during the second and third week post-inoculation. No clinical or behavioral modifications were observed during the eighty-days observation period. Non-specific necropsy findings included pyelonephritis, pneumonitis, liver abscess and eosinophilic spleen infiltrate. All of these findings seem to be unrelated to Junin virus inoculation. No virus was present in organs of animals killed 29, 57 or 85 days post-inoculation. All nine owl monkeys developed serum neutralizing antibodies by day 22. It is concluded that the owl monkey suffers a subclinical infection when inoculated with Junin virus, similar to that seen in other primate species (Saimiri sciureus and Alouatta caraya).
RESUMO
Cuatro primates neotropicales de la especie S. sciureus fueron inoculados con la cepa patogena prototipo XJ del virus Junin a fin de determinar su susceptibilidad a la misma. Dos S. sciureus fueron inoculados por via intramuscular con 10(3) DL50 de dicha cepa, y las dos restantes con 10 (5) DL50. Independentemiente de las dosis de virus administrada, ninguno de los monos inoculados mostro signos clinicos de enfermedad hasta la fecha en que se los sacrifico (entre 82 y 178 dias despues de la infeccion), para realizar estudios histologicos y de persistencia viral. No se detectaron modificaciones significativas en el numero de hematies, reticulocitos, plaquetas y leucocitos. La busqueda de virus en sangre entre los 7 y 36 dias despues de la infeccion, dio resultados negativos. Tampoco se detecto la presencia de virus infeccioso, de antigeno viral o de modificaciones histologicas, en los organos de los animales sacrificados. Tres de los 4 monos infectados desarrollaron anticuerpos neutralizantes humorales antivirus Junin
Assuntos
Feminino , Animais , Arenavirus do Novo Mundo , Febre Hemorrágica Americana , SaimiriAssuntos
Masculino , Feminino , Animais , Prenhez , Reprodução , Comportamento Sexual Animal , SaimiriAssuntos
Masculino , Feminino , Animais , Comportamento Sexual Animal , Prenhez , Reprodução , SaimiriRESUMO
Cuatro primates neotropicales de la especie S. sciureus fueron inoculados con la cepa patogena prototipo XJ del virus Junin a fin de determinar su susceptibilidad a la misma. Dos S. sciureus fueron inoculados por via intramuscular con 10(3) DL50 de dicha cepa, y las dos restantes con 10 (5) DL50. Independentemiente de las dosis de virus administrada, ninguno de los monos inoculados mostro signos clinicos de enfermedad hasta la fecha en que se los sacrifico (entre 82 y 178 dias despues de la infeccion), para realizar estudios histologicos y de persistencia viral. No se detectaron modificaciones significativas en el numero de hematies, reticulocitos, plaquetas y leucocitos. La busqueda de virus en sangre entre los 7 y 36 dias despues de la infeccion, dio resultados negativos. Tampoco se detecto la presencia de virus infeccioso, de antigeno viral o de modificaciones histologicas, en los organos de los animales sacrificados. Tres de los 4 monos infectados desarrollaron anticuerpos neutralizantes humorales antivirus Junin