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Insect pest control can be achieved by the application of RNA interference (RNAi), a key molecular tool in functional genomics. Whereas most RNAi research has focused on insect pests, few studies have been performed on natural enemies. Validating the efficacy of RNAi in natural enemies is crucial for assessing its safety and enabling molecular research on these organisms. Here, we assessed the efficacy of RNAi in the ladybird beetle Eriopis connexa Germar (Coleoptera: Coccinellidae), focusing on genes related to reproduction, such as vitellogenin (Vg) and its receptor (VgR). In the transcriptome of E. connexa, we found one VgR (EcVgR) and two Vg genes (EcVg1 and EcVg2). These genes have been validated by in silico analyses of functional domains and evolutionary relationships. Five-day-old females were injected with 500 ng/µL of a specific double-stranded RNA (dsRNA) (dsEcVg1, dsEcVg2, or dsEcVgR) for RNAi tests, while nonspecific dsRNA (dsGFP or dsAgCE8.1) was used as a control. Interestingly, dsEcVg2 was able to knockdown both Vg genes, while dsEcVg1 could silence only EcVg1. Additionally, the viability of the eggs was significantly reduced when both Vg genes were knocked down at the same time (after treatment with dsEcVg2 or "dsEcVg1+dsEcVg2"). Ultimately, malformed, nonviable eggs were produced when EcVgR was silenced. Interestingly, no dsRNA treatment had an impact on the quantity of eggs laid. Therefore, the feasibility of RNAi in E. connexa has been confirmed, suggesting that this coccinellid is an excellent Neotropical model for molecular research on natural enemies and for studying RNAi nontarget effects.

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The efficiency of RNAi technology in insects varies considerably, particularly in lepidopterans. An important limiting factor of RNAi-mediated gene silencing is the degradation of dsRNA by insect nucleases before cellular uptake. To date, few studies have reported effective gene knockdown in the sugarcane borer Diatraea saccharalis. However, yielding contradictory results when using oral delivery. Further, the RNAi efficiency in D. saccharalis and presumed activity of gut nucleases remain poorly understood. Therefore, we investigated whether gene silencing was feasible via dsRNA feeding in D. saccharalis. Two different genes were tested, juvenile hormone esterase (DsJHE) and chitin synthase 1 (DsCHS1). Discrete knockdown was verified only for DsCHS1 with high dsRNA dosages and long exposure times. Neither mortality nor abnormal phenotypes were observed after treatment with any tested dsRNA. It was also verified that dsRNAs were quickly degraded when incubated with gut juice. Furthermore, we identified four possible nucleases that could reduce the knockdown efficiency in D. saccharalis. Three of them had the endonuclease_NS domain (DsNucleases), and one had the PIN domain (DsREase), with REase-like genes being scarcely represented in databanks. We further remark that DsNuclease1 and DsREase are highly expressed in the larval gut, and DsREase was upregulated as insects were fed with artificial diet (without dsRNA), and also when injected with dsRNA. Conversely, no nuclease was triggered when insects were fed with a sucrose droplet containing dsRNA. Thus, our findings suggest that nuclease activity within the gut is one of the possible reasons for the inefficiency of RNAi in D. saccharalis. Our data may shed light on the challenges to overcome when introducing RNAi as a strategy for controlling lepidopteran pests.

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Abstract Background In Brazil the factors involved in the risk of death in patients with COVID-19 have not been well established. Objective To analyze whether elevations of high-sensitivity troponin I (hTnI) levels influence the mortality of patients with COVID-19. Methods Clinical and laboratory characteristics of hospitalized patients with COVID-19 were collected upon hospital admission. Univariate and binary logistic regression analyzes were performed to assess the factors that influence mortality. P-value<0.05 was considered significant. Results This study analyzed192 patients who received hospital admission between March 16 and June 2, 2020 and who were discharged or died by July 2, 2020. The mean age was 70±15 years, 80 (41.7%) of whom were women. In comparison to those who were discharged, the 54 (28.1%) who died were older (79±12 vs 66±15years; P=0.004), and with a higher Charlson´s index (5±2 vs 3±2; P=0.027). More patients, aged≥60years (P <0.0001), Charlson´s index>1 (P=0.004), lung injury>50% in chest computed tomography (P=0.011), with previous coronary artery disease (P=0.037), hypertension (P=0.033), stroke (P=0.008), heart failure (P=0.002), lymphocytopenia (P=0.024), high D-dimer (P=0.024), high INR (P=0.003), hTnI (P<0.0001), high creatinine (P<0.0001), invasive mechanical ventilation (P<0.0001), renal replacement therapy (P<0.0001), vasoactive amine (P<0.0001), and transfer to the ICU (P=0.001), died when compared to those who were discharged. In logistic regression analysis, elevated hTnI levels (OR=9.504; 95% CI=1.281-70.528; P=0.028) upon admission, and the need for mechanical ventilation during hospitalization (OR=46.691; 95% CI=2.360-923.706; P=0.012) increased the chance of in-hospital mortality. Conclusion This study suggests that in COVID-19 disease, myocardial injury upon hospital admission is a harbinger of poor prognosis.

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BACKGROUND: Insecticide resistance in arthropods is an inherited trait that has become a major cause of insect pest control failure. Monitoring the level of susceptibility and characterization of the type of resistance of key pest species aims to determine the risk of resistance selection in time to take action to mitigate control failures. Seven populations of the boll weevil, Anthonomus grandis grandis, collected from cotton fields in the Semiarid and Cerrado areas of Brazil, were screened for their resistance to malathion and beta-cyfluthrin, insecticides widely recommended for control of boll weevil and other pests. RESULTS: The levels of adult mortality were variable for beta-cyfluthrin (0-82%) but invariant (100%) for malathion. Bioassays of concentration-mortality were used to determine lethal concentrations (LCs) for each insecticide. The LC-values corroborate the lack of resistance to field rates of malathion but high levels of resistance to beta-cyfluthrin from 62.7- to 439.7-fold. Weevils resistant to beta-cyfluthrin were found through genome sequencing to possess a kdr mutation through the L1014F substitution in the voltage gated-sodium channel gene. CONCLUSIONS: This study found boll weevil resistance to beta-cyfluthrin to be not mediated by carboxylesterases, but with cross-resistance to DDT and carbaryl, and kdr mutation as the major mechanism of the resistance in our samples. Caution is recommended in further use of beta-cyfluthrin against boll weevil due to potential resistance. Monitoring studies using other boll weevil populations are recommended to determine the geographic pattern and extent of pyrethroid resistance. © 2021 Society of Chemical Industry.

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BACKGROUND: The hemolymph and insect gut together have an essential role in the immune defense against microorganisms, including the production of antimicrobial peptides (AMP). AMPs are mainly induced by two specific signaling pathways, Toll and immune deficiency (IMD). Here, we characterize the expression profile of four genes from both pathways and describe the importance of AgraRelish in the immune defense of Anthonomus grandis against the entomopathogenic fungus Metarhizium anisopliae by RNA interference (RNAi). RESULTS: To characterize the pathway that is activated early during the A. grandis-M. anisopliae interaction, we assessed the expression profiles of AgraMyD88 and AgraDorsal (Toll pathway), AgraIMD and AgraRelish (IMD pathway), and several AMP genes. Interestingly, we found that IMD pathway genes are upregulated early, and Toll pathway genes are upregulated just 3 days after inoculation (DAI). Furthermore, nine AMPs were upregulated 24 h after fungus inoculation, including attacins, cecropins, coleoptericins, and defensins. AgraRelish knockdown resulted in a reduction in median lethal time (LT50 ) for M. anisopliae-treated insects of around 2 days compared to control treatments. In addition, AgraRelish remained knocked down at 3 DAI. Finally, we identified that AgraRelish knockdown increased fungal loads at 2 DAI compared to control treatments, possibly indicating a faster infection. CONCLUSIONS: Our data indicate the influence of the IMD pathway on the antifungal response in A. grandis. Combining biocontrol and RNAi could significantly improve cotton boll weevil management. Hence, AgraRelish is a potential target for the development of biotechnological tools aimed at improving the efficacy of M. anisopliae against A. grandis.

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Natural products are a valuable source of biologically active compounds and continue to play an important role in modern drug discovery due to their great structural diversity and unique biological properties. Brazilian biodiversity is one of the most extensive in the world and could be an effective source of new chemical entities for drug discovery. Mosquitoes are vectors for the transmission of dengue, Zika, chikungunya, yellow fever, and many other diseases of public health importance. These diseases have a major impact on tropical and subtropical countries, and their incidence has increased dramatically in recent decades, reaching billions of people at risk worldwide. The prevention of these diseases is mainly through vector control, which is becoming more difficult because of the emergence of resistant mosquito populations to the chemical insecticides. Strategies to provide efficient and safe vector control are needed, and secondary metabolites from plant species from the Brazilian biodiversity, especially Cerrado, that are biologically active for mosquito control are herein highlighted. Also, this is a literature revision of targets as insights to promote advances in the task of developing active compounds for vector control. In view of the expansion and occurrence of arboviruses diseases worldwide, scientific reviews on bioactive natural products are important to provide molecular models for vector control and contribute with effective measures to reduce their incidence.

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The cotton boll weevil, Anthonomus grandis, is the most economically important pest of cotton in Brazil. Pest management programs focused on A. grandis are based mostly on the use of chemical insecticides, which may cause serious ecological impacts. Furthermore, A. grandis has developed resistance to some insecticides after their long-term use. Therefore, alternative control approaches that are more sustainable and have reduced environmental impacts are highly desirable to protect cotton crops from this destructive pest. RNA interference (RNAi) is a valuable reverse genetics tool for the investigation of gene function and has been explored for the development of strategies to control agricultural insect pests. This study aimed to evaluate the biological role of the Laccase2 (AgraLac2) gene in A. grandis and its potential as an RNAi target for the control of this insect pest. We found that AgraLac2 is expressed throughout the development of A. grandis with significantly higher expression in pupal and adult developmental stages. In addition, the immunolocalization of the AgraLac2 protein in third-instar larvae using specific antibodies revealed that AgraLac2 is distributed throughout the epithelial tissue, the cuticle and the tracheal system. We also verified that the knockdown of AgraLac2 in A. grandis resulted in an altered cuticle tanning process, molting defects and arrested development. Remarkably, insects injected with dsAgraLac2 exhibited defects in cuticle hardening and pigmentation. As a consequence, the development of dsAgraLac2-treated insects was compromised, and in cases of severe phenotypic defects, the insects subsequently died. On the contrary, insects subjected to control treatments did not show any visible phenotypic defects in cuticle formation and successfully molted to the pupal and adult stages. Taken together, our data indicate that AgraLac2 is involved in the cuticle tanning process in A. grandis and may be a promising target for the development of RNAi-based technologies.

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Coffee production is a global industry valued at approximately 173 billion US dollars. One of the main challenges facing coffee production is the management of the coffee berry borer (CBB), Hypothenemus hampei, which is considered the primary arthropod pest of coffee worldwide. Current control strategies are inefficient for CBB management. Although biotechnological alternatives, including RNA interference (RNAi), have been proposed in recent years to control insect pests, characterizing the genetics of the target pest is essential for the successful application of these emerging technologies. In this study, we employed RNA-seq to obtain the transcriptome of three developmental stages of the CBB (larva, female and male) to increase our understanding of the CBB life cycle in relation to molecular features. The CBB transcriptome was sequenced using Illumina Hiseq and assembled de novo. Differential gene expression analysis was performed across the developmental stages. The final assembly produced 29,434 unigenes, of which 4,664 transcripts were differentially expressed. Genes linked to crucial physiological functions, such as digestion and detoxification, were determined to be tightly regulated between the reproductive and nonreproductive stages of CBB. The data obtained in this study help to elucidate the critical roles that several genes play as regulatory elements in CBB development.

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Vitellogenin (Vg), a yolk protein precursor, is the primary egg nutrient source involved in insect reproduction and embryo development. The Cotton Boll weevil (CBW) Anthonomus grandis Boheman, the most important cotton pest in Americas, accumulates large amounts of Vg during reproduction. However, the precise role of this protein during embryo development in this insect remains unknown. Herein, we investigated the effects of vitellogenin (AgraVg) knockdown on the egg-laying and egg viability in A. grandis females, and also characterized morphologically the unviable eggs. AgraVg transcripts were found during all developmental stages of A. grandis, with highest abundance in females. Silencing of AgraVg culminated in a significant reduction in transcript amount, around 90%. Despite this transcriptional reduction, egg-laying was not affected in dsRNA-treated females but almost 100% of the eggs lost their viability. Eggs from dsRNA-treated females showed aberrant embryos phenotype suggesting interference at different stages of embryonic development. Unlike for other insects, the AgraVg knockdown did not affect the egg-laying ability of A. grandis, but hampered A. grandis reproduction by perturbing embryo development. We concluded that the Vg protein is essential for A. grandis reproduction and a good candidate to bio-engineer the resistance against this devastating cotton pest.

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AIM: Childhood maltreatment (CM) has been related to a persistent reprograming of stress-response. Copeptin is a marker of hypothalamic-pituitary-adrenal axis activation; however, few studies have examined copeptin levels in children exposed to CM. The aim of this study was to compare serum copeptin levels in children reporting child abuse and/or neglect and children with no history of CM. METHODS: This study included 65 children with a positive history of moderate to severe CM, as reported by themselves and their parent(s) during a clinical interview, and 71 children with no history of CM as a comparison group. CM was considered moderate to severe based on the child-reported frequency of being exposed to events related to sexual abuse, physical abuse, emotional abuse, emotional neglect, and/or physical neglect. Child psychopathology symptoms were assessed using the Child Behavior Checklist (CBCL). We measured serum copeptin concentration using enzyme-linked immunosorbent assay. RESULTS: Children exposed to CM exhibited higher levels of serum copeptin compared to children without CM when controlling for sex, age, and psychiatric morbidity. The CBCL total score, including internalizing and externalizing symptoms, was higher in children with CM. We found no correlation between copeptin and CBCL scores for internalizing symptoms and externalizing symptoms. CONCLUSION: CM is associated with copeptin serum levels independently of age, sex, and symptom severity. Copeptin is a promising new biomarker for children with a history of abuse and/or neglect.

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Plant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases.

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No Brasil, os hemocentros têm encontrado dificuldades em manter um nível de estoque de bolsas de sangue que atendam às demandas da população. Assim, o Ministério da Saúde (MS) elabora anualmente a Campanha Nacional de Doação de Sangue. Sabendo-se que a comunicação na Campanha Nacional de Doação de Sangue são realizadas a partir de materiais educativos impressos, além de recursos audiovisuais, faz-se necessário conhecer o processo comunicativo realizado através destes. Objetivo: Realizar a análise do conteúdo de aprendizagem de um cartaz da Campanha Nacional de Doação de Sangue. Materiais e Método: Trata-se de uma pesquisa documental realizada a partir da análise de conteúdo de aprendizagem sob a perspectiva de Antoni Zabala de um material educativo impresso elaborado pelo Ministério da Saúde (MS) para a Campanha Nacional de Doação de Sangue do ano de 2015. Resultados: O material educativo impresso analisado buscou, através dos seus conteúdos, estimular a doação de sangue apresentando uma imagem que remete ao gesto de solidariedade e gratidão. Segundo a análise realizada, foram encontrados conteúdos atitudinal e conceitual, atitudinal e procedimental e conceitual, atitudinal e factual. Conclusão: o presente estudo possibilitou entender a importância da comunicação nos processos de ensino-aprendizagem demonstrando que não existe uma única corrente de interpretação e construção deste processo sendo necessário agregar à utilização do material educativo outras formas de sensibilização dos doadores para que a participação de todos possa ocorrer de maneira efetiva, doando sangue e compartilhando vidas num gesto de solidariedade.

In Brazil, blood centers have found it difficult to maintain a stock level of blood bags that meet the demands of the population. Thus, the health ministry shall draw up annually national blood donation campaigns. Knowing that communication in national blood donation campaigns are made from printed educational materials, and audio visual resources, it is necessary to know the communication process performed by these. Objective: The purpose of this article is perform analysis of learning content of a poster of the national donation campaign. Materials and Methods: It is a documentary research performed from the learning content analysis under Antoni Zabala perspective of an educational printed material prepared by the Ministry for 2015 national blood donation campaign. Results: The analyzed printed educational material sought, through its contents, stimulate blood donation presenting an image that refers to the gesture of solidarity and gratitude. According to the analysis carried out, attitudinal and conceptual, attitudinal and procedural and conceptual, attitudinal and factual contents were found. Conclusion: Thus, this study made it possible to understand the importance of communication in teaching and learning processes demonstrating that there is no single current interpretation and construction of this process it is necessary to add the use of educational material other forms of awareness of donors to the participation of all can effectively occur, donating blood and sharing lives in a solidarity gesture.

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Food additives were used to improve meat characteristics of Brangus heifers supplemented on pasture daily (0.32% LW). The treatments were: supplement (with no additives); calcareous seaweed flour (Lithothamnium calcareum) added to the supplement; vitamin E and organic selenium added to the supplement; protected fat added to the supplement; a subcutaneous application of vitamin D seven days before slaughter + supplement; and the association of vitamin E, organic selenium and protected fat added to the supplement + one subcutaneous dose of vitamin D seven days before slaughter. The animals were supplemented by 109.80±11,71 days and were slaughtered when they reached approximately 337.95±20,56kg of LW. The experimental design was completely randomized with six treatments and 25 repetitions. TheLongissimus dorsi muscle’s chemical composition did not differ between treatments (P>0.05). The use of seaweed flour, vitamin D, protected fat and the association of additives has the potential to improve the concentration of some unsaturated fatty acids in the Brangus heifers’ meat (P˂0.05), but without improving the level of unsaturated fatty acids class. We conclude that the use of additives in heifers finished in the pasture/supplement system has no effect on the meat quality, but only, isolated outcomes, on the content of some fatty acids.

Os aditivos alimentares foram utilizados com o intuito de melhorar as características da carne de novilhas Brangus suplementadas a pasto (0,32% PV). Os tratamentos foram: suplemento base (sem aditivos); farinha de algas calcárias (Lithothamnium calcareum) adicionada ao suplemento base; vitamina E e selênio orgânico adicionados ao suplemento base; gordura protegida adicionada ao suplemento base; uma aplicação por via subcutânea de vitamina D sete dias antes do abate + suplemento base; e uma associação de vitamina E, selênio orgânico e gordura protegida adicionados ao suplemento base + uma aplicação por via subcutânea de vitamina D sete dias antes do abate. Os animais foram suplementados por 109,80±11,71 dias, quando foram abatidos ao alcançarem aproximadamente 337,95±20,56kg de PV. O delineamento experimental foi inteiramente casualizado, com seis tratamentos e 25 repetições. A composição centesimal do músculo Longissimus dorsi não diferiu entre os tratamentos (P>0,05). A utilização de farinha de algas calcárias, vitamina D, gordura protegida e aditivos associados tem potencial de aumentar a concentração de alguns ácidos graxos insaturados da carne de novilhas Brangus (P˂0,05), sem, no entanto, melhorar o teor total da classe de insaturados. Conclui-se que a utilização de aditivos alimentares em novilhas terminadas em sistema de suplementação a pasto não tem efeitos sobre a qualidade da carne, exceto, de maneira isolada, sobre o teor de alguns ácidos graxos avaliados.

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AbstractFood additives were used to improve meat characteristics of Brangus heifers supplemented on pasture daily (0.32% LW). The treatments were: supplement (with no additives); calcareous seaweed flour (Lithothamnium calcareum) added to the supplement; vitamin E and organic selenium added to the supplement; protected fat added to the supplement; a subcutaneous application of vitamin D seven days before slaughter + supplement; and the association of vitamin E, organic selenium and protected fat added to the supplement + one subcutaneous dose of vitamin D seven days before slaughter. The animals were supplemented by 109.80±11,71 days and were slaughtered when they reached approximately 337.95±20,56kg of LW. The experimental design was completely randomized with six treatments and 25 repetitions. TheLongissimus dorsi muscles chemical composition did not differ between treatments (P>0.05). The use of seaweed flour, vitamin D, protected fat and the association of additives has the potential to improve the concentration of some unsaturated fatty acids in the Brangus heifers meat (P0.05), but without improving the level of unsaturated fatty acids class. We conclude that the use of additives in heifers finished in the pasture/supplement system has no effect on the meat quality, but only, isolated outcomes, on the content of some fatty acids.

ResumoOs aditivos alimentares foram utilizados com o intuito de melhorar as características da carne de novilhas Brangus suplementadas a pasto (0,32% PV). Os tratamentos foram: suplemento base (sem aditivos); farinha de algas calcárias (Lithothamnium calcareum) adicionada ao suplemento base; vitamina E e selênio orgânico adicionados ao suplemento base; gordura protegida adicionada ao suplemento base; uma aplicação por via subcutânea de vitamina D sete dias antes do abate + suplemento base; e uma associação de vitamina E, selênio orgânico e gordura protegida adicionados ao suplemento base + uma aplicação por via subcutânea de vitamina D sete dias antes do abate. Os animais foram suplementados por 109,80±11,71 dias, quando foram abatidos ao alcançarem aproximadamente 337,95±20,56kg de PV. O delineamento experimental foi inteiramente casualizado, com seis tratamentos e 25 repetições. A composição centesimal do músculo Longissimus dorsi não diferiu entre os tratamentos (P>0,05). A utilização de farinha de algas calcárias, vitamina D, gordura protegida e aditivos associados tem potencial de aumentar a concentração de alguns ácidos graxos insaturados da carne de novilhas Brangus (P0,05), sem, no entanto, melhorar o teor total da classe de insaturados. Conclui-se que a utilização de aditivos alimentares em novilhas terminadas em sistema de suplementação a pasto não tem efeitos sobre a qualidade da carne, exceto, de maneira isolada, sobre o teor de alguns ácidos graxos avaliados.

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Food additives were used to improve meat characteristics of Brangus heifers supplemented on pasture daily (0.32% LW). The treatments were: supplement (with no additives); calcareous seaweed flour (Lithothamnium calcareum) added to the supplement; vitamin E and organic selenium added to the supplement; protected fat added to the supplement; a subcutaneous application of vitamin D seven days before slaughter + supplement; and the association of vitamin E, organic selenium and protected fat added to the supplement + one subcutaneous dose of vitamin D seven days before slaughter. The animals were supplemented by 109.80±11,71 days and were slaughtered when they reached approximately 337.95±20,56kg of LW. The experimental design was completely randomized with six treatments and 25 repetitions. TheLongissimus dorsi muscles chemical composition did not differ between treatments (P>0.05). The use of seaweed flour, vitamin D, protected fat and the association of additives has the potential to improve the concentration of some unsaturated fatty acids in the Brangus heifers meat (P˂0.05), but without improving the level of unsaturated fatty acids class. We conclude that the use of additives in heifers finished in the pasture/supplement system has no effect on the meat quality, but only, isolated outcomes, on the content of some fatty acids.(AU)

Os aditivos alimentares foram utilizados com o intuito de melhorar as características da carne de novilhas Brangus suplementadas a pasto (0,32% PV). Os tratamentos foram: suplemento base (sem aditivos); farinha de algas calcárias (Lithothamnium calcareum) adicionada ao suplemento base; vitamina E e selênio orgânico adicionados ao suplemento base; gordura protegida adicionada ao suplemento base; uma aplicação por via subcutânea de vitamina D sete dias antes do abate + suplemento base; e uma associação de vitamina E, selênio orgânico e gordura protegida adicionados ao suplemento base + uma aplicação por via subcutânea de vitamina D sete dias antes do abate. Os animais foram suplementados por 109,80±11,71 dias, quando foram abatidos ao alcançarem aproximadamente 337,95±20,56kg de PV. O delineamento experimental foi inteiramente casualizado, com seis tratamentos e 25 repetições. A composição centesimal do músculo Longissimus dorsi não diferiu entre os tratamentos (P>0,05). A utilização de farinha de algas calcárias, vitamina D, gordura protegida e aditivos associados tem potencial de aumentar a concentração de alguns ácidos graxos insaturados da carne de novilhas Brangus (P˂0,05), sem, no entanto, melhorar o teor total da classe de insaturados. Conclui-se que a utilização de aditivos alimentares em novilhas terminadas em sistema de suplementação a pasto não tem efeitos sobre a qualidade da carne, exceto, de maneira isolada, sobre o teor de alguns ácidos graxos avaliados.(AU)

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Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the GenBank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a number of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect's biology and to guide the development of new strategies for insect-pest control.

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AbstractFood additives were used to improve meat characteristics of Brangus heifers supplemented on pasture daily (0.32% LW). The treatments were: supplement (with no additives); calcareous seaweed flour (Lithothamnium calcareum) added to the supplement; vitamin E and organic selenium added to the supplement; protected fat added to the supplement; a subcutaneous application of vitamin D seven days before slaughter + supplement; and the association of vitamin E, organic selenium and protected fat added to the supplement + one subcutaneous dose of vitamin D seven days before slaughter. The animals were supplemented by 109.80±11,71 days and were slaughtered when they reached approximately 337.95±20,56kg of LW. The experimental design was completely randomized with six treatments and 25 repetitions. TheLongissimus dorsi muscles chemical composition did not differ between treatments (P>0.05). The use of seaweed flour, vitamin D, protected fat and the association of additives has the potential to improve the concentration of some unsaturated fatty acids in the Brangus heifers meat (P0.05), but without improving the level of unsaturated fatty acids class. We conclude that the use of additives in heifers finished in the pasture/supplement system has no effect on the meat quality, but only, isolated outcomes, on the content of some fatty acids.

ResumoOs aditivos alimentares foram utilizados com o intuito de melhorar as características da carne de novilhas Brangus suplementadas a pasto (0,32% PV). Os tratamentos foram: suplemento base (sem aditivos); farinha de algas calcárias (Lithothamnium calcareum) adicionada ao suplemento base; vitamina E e selênio orgânico adicionados ao suplemento base; gordura protegida adicionada ao suplemento base; uma aplicação por via subcutânea de vitamina D sete dias antes do abate + suplemento base; e uma associação de vitamina E, selênio orgânico e gordura protegida adicionados ao suplemento base + uma aplicação por via subcutânea de vitamina D sete dias antes do abate. Os animais foram suplementados por 109,80±11,71 dias, quando foram abatidos ao alcançarem aproximadamente 337,95±20,56kg de PV. O delineamento experimental foi inteiramente casualizado, com seis tratamentos e 25 repetições. A composição centesimal do músculo Longissimus dorsi não diferiu entre os tratamentos (P>0,05). A utilização de farinha de algas calcárias, vitamina D, gordura protegida e aditivos associados tem potencial de aumentar a concentração de alguns ácidos graxos insaturados da carne de novilhas Brangus (P0,05), sem, no entanto, melhorar o teor total da classe de insaturados. Conclui-se que a utilização de aditivos alimentares em novilhas terminadas em sistema de suplementação a pasto não tem efeitos sobre a qualidade da carne, exceto, de maneira isolada, sobre o teor de alguns ácidos graxos avaliados.

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Consumers are more interested in having healthier and better quality products. The diet offered to animals can strongly affect the composition of meat. Thus, this study aimed to evaluate the effect of different levels of concentrate on lipid profile (in natura meat) and cholesterol content (in natura and cooked meat) of ewe lambs meat cuts. Therefore, twenty-four lambs with a mean weight of 23.1 ± 2.1 kg were confined and fed different levels of a concentrate (20, 40, 60 and 80%) for 120 days. The diets consisted of tifton hay and concentrate based on corn and soybean meal. The muscles triceps brachii (shoulder), longissimus dorsi (loin) and semimembranosus (leg) represented the respective cuts that were analyzed. Increased levels of concentrate decreased only C18:3 (5.82 to 0.20 mg/100 g) inmeat and without affecting saturated fatty acids (SFA), unsaturated (UFA), monounsaturated (MUFA), polyunsaturated (PUFA), both desirable and total levels. Levels of concentrate had no effect on UFA/ SFA (1.01), MUFA/SFA (0.97), PUFA/SFA (0.04) ratios, or the atherogenic (AI) and thrombogenic index (TI), nor on the content cholesterol in natura and cooked meat. The meat cuts had different lipid profiles, IA, IT and cholesterol content. Concentrate levels slightly alter the lipid profile and do not alter the cholesterol content, while meat cuts are different for these components.(AU)

Os consumidores estão cada vez mais interessados em consumir produtos saudáveis e de melhor qualidade.A dieta oferecida aos animais pode afetar fortemente a composição da carne. Desta forma, objetivou-se avaliar o efeito dos diferentes níveis de concentrado sobre o perfil lipídico (carne in natura) e o teor decolesterol (carne in natura e assada) dos cortes cárneos de borregas. Para isso, vinte e quatro borregas com peso médio de 23,1 ± 2,1 kg foram confinadas e alimentadas com diferentes níveis de concentrado(20, 40, 60 e 80%) por 120 dias. As dietas consistiram de feno Tifton e concentrado a base de milho e farelo de soja. Os músculos tríceps brachii, (paleta), longissimus dorsi (lombo) e semimembranosus (pernil) representaram os respectivos cortes nas análises. O aumento dos níveis de concentrado diminuiu apenas o C18:3 (5,82 para 0,20 mg/100 g de carne fresca) nas carnes e não alteraram os ácidos graxossaturados (AGS), insaturados (AGI), monoinsaturados (AGMI), poliinsaturados (AGPI), desejáveis e totais. Os níveis de concentrado não afetaram as relações AGI/AGS (1,01), AGMI/AGS (0,97), AGPI/AGS (0,04), índices aterogênico (IA) e trombogênico (IT) e nem o teor de colesterol na carne in natura e assada. Os cortes cárneos apresentaram diferenças para o perfil lipídico, IA, IT e teor colesterol. Osníveis de concentrado pouco alteram o perfil lipídico e não alteram os teores de colesterol, enquanto que os cortes cárneos são diferentes para estes componentes.(AU)

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OBJECTIVE: To assess differences in executive functioning between children and adolescents with attention-deficit/hyperactivity disorder (ADHD) comorbid or not with bipolar disorder (BD), and to study the neuropsychological profile of subjects with the comorbidity in a clinical sample from a developing country. METHOD: Case-control study comparing 23 participants with BD + ADHD and 85 ADHD-only subjects aged 6 to 17 years old. Both groups were drug-free. Executive function domains were assessed with the Stroop Test, the Wisconsin Card Sorting Test, and the Continuous Performance Test II. RESULTS: The group with juvenile BD + ADHD showed a significantly worse performance on the Stroop task, including time in color (p = 0.002), time in color-word (p < 0.001), interference, number or errors in color and color-word (p = 0.001), and number of errors in word cards (p = 0.028). No between-group differences were found in other tests. CONCLUSIONS: Our findings suggest that ADHD-only and ADHD + BD do not show differences in inhibitory control and set-shifting domains. However, children and adolescents with BD and comorbid ADHD show greater impairment in processing speed and interference control. This suggests a potentially higher impairment in the dorsolateral prefrontal cortex and may be a potential neuropsychological signature of juvenile BD comorbid with ADHD.

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RESUMO

Objective: To assess differences in executive functioning between children and adolescents with attention-deficit/hyperactivity disorder (ADHD) comorbid or not with bipolar disorder (BD), and to study the neuropsychological profile of subjects with the comorbidity in a clinical sample from a developing country. Method: Case-control study comparing 23 participants with BD + ADHD and 85 ADHD-only subjects aged 6 to 17 years old. Both groups were drug-free. Executive function domains were assessed with the Stroop Test, the Wisconsin Card Sorting Test, and the Continuous Performance Test II. Results: The group with juvenile BD + ADHD showed a significantly worse performance on the Stroop task, including time in color (p = 0.002), time in color-word (p < 0.001), interference, number or errors in color and color-word (p = 0.001), and number of errors in word cards (p = 0.028). No between-group differences were found in other tests. Conclusions: Our findings suggest that ADHD-only and ADHD + BD do not show differences in inhibitory control and set-shifting domains. However, children and adolescents with BD and comorbid ADHD show greater impairment in processing speed and interference control. This suggests a potentially higher impairment in the dorsolateral prefrontal cortex and may be a potential neuropsychological signature of juvenile BD comorbid with ADHD. .

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