RESUMO
Ultrastructural and morphometric changes in oocyte and surrounding granulosa cells during armadillo follicular growth are described. Primordial, intermediary, early and late primary, secondary, tertiary and preovulatory Graafian follicles were found. From primordial to Graafian follicle, granulosa cells increase in height, become multilayered while fluid-filled spaces arise among them in the tertiary follicle stage. As the follicle expands the oocyte is located eccentrically and grows in size. From primordial to secondary stage organelles develop in number and complexity and many vesicles start arising in the cytoplasm at the secondary stage. From tertiary follicle stage the number of mitochondria decreases. In follicular cells, secreting-related organelles expand, but mitochondrial number decreases according to the follicle expansion. Intercellular junctions between both cell types increase. The morphometrical study shows a biphasic pattern of oocyte and follicle growth. The morphological baseline here provided will be essential for further comprehension of the reproductive biology in armadillos.
Assuntos
Tatus/anatomia & histologia , Folículo Ovariano/ultraestrutura , Animais , Tatus/crescimento & desenvolvimento , Feminino , Folículo Ovariano/crescimento & desenvolvimentoRESUMO
Ultrastructural and morphometric changes in oocyte and surrounding granulosa cells during armadillo follicular growth are described. Primordial, intermediary, early and late primary, secondary, tertiary and preovulatory Graafian follicles were found. From primordial to Graafian follicle, granulosa cells increase in height, become multilayered while fluid-filled spaces arise among them in the tertiary follicle stage. As the follicle expands the oocyte is located eccentrically and grows in size. From primordial to secondary stage organelles develop in number and complexity and many vesicles start arising in the cytoplasm at the secondary stage. From tertiary follicle stage the number of mitochondria decreases. In follicular cells, secreting-related organelles expand, but mitochondrial number decreases according to the follicle expansion. Intercellular junctions between both cell types increase. The morphometrical study shows a biphasic pattern of oocyte and follicle growth. The morphological baseline here provided will be essential for further comprehension of the reproductive biology in armadillos
Assuntos
Animais , Feminino , Tatus , Folículo Ovariano/ultraestrutura , Tatus , Folículo Ovariano/crescimento & desenvolvimentoRESUMO
Armadillos are relictual mammals important as models for biomedical studies. They contain adaptative and primitive characteristics in both anatomical and physiological aspects. In this study we describe the splenic histology and cytology of the "mulita," Dasypus hybridus. Organ samples were processed for light and electron microscopy study. The microanatomy of the organ samples as well as their different cell types are described. The spleen is non-sinusoidal, with the typical arrangement for storage functions. White pulp is lightly diffuse. Red pulp is a meshwork of circulating, immunocompetent and hemopoietic cells. Differences with other studied members of the group are discussed. The general structure of the organ agrees with the semi-fossorial habit of the species. Persistence of myeloid activity in the adult suggests the existence of specific inductive functions of the stroma. Better knowledge of this fact may give further insight on the phylogeny of hemopoiesis.