RESUMO
Crude extracts of Haplopappus sonorensis (A. Gray) S.F. Blake (Asteraceae), showed activity against Mycobacterium tuberculosis H(37)Rv. By assay-guided fractionation, 5-hydroxy-3,7,4'-trimethoxyflavone (1). 5,7-dihydroxy-3,4'-dimethoxyflavone (2). and 5,4'-dihydroxy-3,7-dimethoxyflavone (3). were identified as the antimycobacterial principles. Compound 2 was the most active compound.
Assuntos
Antituberculosos/farmacologia , Flavonoides/farmacologia , Haplopappus , Mycobacterium tuberculosis/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Humanos , Testes de Sensibilidade Microbiana , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
A total of 227 marine isolates of ubiquitous fungi were cultivated on different media and the secondary metabolite content of the extracts (ethyl acetate/chloroform/methanol 3:2:1) characterized by HPLC. The fungi were secured from animals, plants and sediments of Venezuelan waters (0-10 m) including mangroves and lagoonal areas. The extracts were tested for antibacterial activity. A total of 7 were active towards Vibrio parahaemolyticus and 55 towards Staphylococcus aureus, representing 18 different fungal species from 8 ascomycetous genera. For 61 strains of Penicillium citrinum antibacterial activity correlated well with content of secondary metabolites as measured by HPLC. Thirteen isolates of Penicillium steckii produced very similar profiles of secondary metabolites and 6 of these had activity against either V. parahaemolyticus or S. aureus or both.
Assuntos
Antibacterianos/farmacologia , Fungos/metabolismo , Sedimentos Geológicos/microbiologia , Água do Mar/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Vibrio parahaemolyticus/efeitos dos fármacos , Antibacterianos/análise , Antibacterianos/metabolismo , Cromatografia Líquida de Alta Pressão , Fungos/isolamento & purificação , Testes de Sensibilidade Microbiana , Penicillium/isolamento & purificação , Penicillium/metabolismo , Venezuela , Microbiologia da ÁguaRESUMO
Two new flavones were isolated from the medicinal plant Calliandra californica, 7,2',4',5'-tetramethoxyflavone [1] and 5-hydroxy-7,2',4',5'-tetramethoxyflavone [2]. The structures were determined from spectral evidence and both compounds were synthesized. Compound 2 exhibited antimicrobial activity for two test bacterial strains.
Assuntos
Anti-Infecciosos/isolamento & purificação , Flavonoides/isolamento & purificação , Plantas Medicinais/química , Antibacterianos , Anti-Infecciosos/farmacologia , Bacillus subtilis/efeitos dos fármacos , California , Candida albicans/efeitos dos fármacos , Flavonoides/farmacologia , Testes de Sensibilidade Microbiana , Staphylococcus aureus/efeitos dos fármacosRESUMO
The medicinal plant Lepechinia hastata, used as a remedy against uterine infections in Baja California Sur (Mexico), was shown to contain carnosol as the main diterpenoid secondary metabolite. Carnosol has potent in vitro antimicrobial activity. Detailed spectroscopical properties of carnosol are presented.