RESUMO
INTRODUCTION: Mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. IgG antibodies against dengue virus (DENV-IgG) are indicators of previous exposure. The risk of DENV transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology. OBJECTIVE: To determine the effect of the presence of Aedes aegypti mosquitos in different stages in households and the history of dengue exposure on vector-human contact determined by the level of anti-salivary protein antibodies in people living in a Colombian endemic area. MATERIALS AND METHODS: A pilot study of 58 households and 55 human subjects was conducted in Norte de Santander, Colombia. A questionnaire for socioeconomic factors was administered and houses were examined for the presence of Ae. aegypti specimens in the aquatic stages. The level of DENV-IgG antibodies (DENV-IgG), in addition to IgG and IgM anti- Ae. aegypti salivary gland extract (SGE) antibodies (SGE-IgG, SGE-IgM) were evaluated by ELISA using blood collected in filter paper. RESULTS: We found a significant higher level of SGE-IgG antibodies in subjects living in houses with Ae. aegypti in aquatic stages. We also found a higher concentration of SGE-IgG antibodies in people exposed to DENV, a positive correlation between IgM-SGE and IgG-DENV and a negative correlation with IgG-SGE. CONCLUSION: Anti-salivary proteins antibodies are consistent with the presence of Ae. aegypti aquatic stages inside houses and DENV-IgG antibodies concentrations.
Assuntos
Aedes/imunologia , Anticorpos Antivirais/sangue , Vírus da Dengue/imunologia , Dengue/epidemiologia , Doenças Endêmicas , Exposição Ambiental , Imunoglobulina G/sangue , Proteínas de Insetos/imunologia , Insetos Vetores/imunologia , Proteínas e Peptídeos Salivares/imunologia , Adolescente , Adulto , Aedes/crescimento & desenvolvimento , Aedes/virologia , Idoso , Animais , Anticorpos Antivirais/imunologia , Criança , Pré-Escolar , Colômbia/epidemiologia , Dengue/transmissão , Vírus da Dengue/classificação , Ensaio de Imunoadsorção Enzimática , Feminino , Habitação , Humanos , Imunoglobulina G/imunologia , Insetos Vetores/virologia , Larva , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Fatores Socioeconômicos , Inquéritos e Questionários , Abastecimento de Água , Adulto JovemRESUMO
Dengue virus (DENV) is the most important mosquito-transmitted flavivirus that is transmitted throughout the tropical and subtropical regions of the world. The primary mosquito vector of DENV in urban locations is Aedes aegypti. Key to understanding the transmission of DENV is the relationship between pathogen and vector. Accordingly, we report our preliminary characterization of the differentially expressed proteins from Ae. aegypti mosquitoes after DENV infection. We investigated the virus-vector interaction through changes in the proteome of the salivary glands of mosquitoes with disseminated DENV serotype 2 (DENV-2) infections using two-dimensional gel electrophoresis and identification by mass spectrometry. Our findings indicate that DENV-2 infection in the Ae. aegypti salivary gland alters the expression of structural, secreted, and metabolic proteins. These changes in the salivary gland proteome highlight the virally influenced environment caused by a DENV-2 infection and warrant additional investigation to determine if these differences extend to the expectorated saliva.
Assuntos
Aedes/metabolismo , Vírus da Dengue , Insetos Vetores/metabolismo , Glândulas Salivares/metabolismo , Aedes/virologia , Animais , Dengue/transmissão , Dengue/virologia , Eletroforese em Gel Bidimensional , Humanos , Insetos Vetores/virologia , Espectrometria de Massas , Glândulas Salivares/virologiaRESUMO
Norte de Santander is a region in Colombia with a high incidence of dengue virus (DENV). In this study, we examined the serum concentration of anti-Aedes salivary gland extract (SGE) antibodies as a biomarker of DENV infection and transmission, and assessed the duration of anti-SGE antibody concentration after exposure to the vector ceased. We also determined whether SGE antibody concentration could differentiate between positive and negative DENV infected individuals and whether there are differences in exposure for each DENV serotype. We observed a significant decrease in the concentration of IgG antibodies at least 40 days after returning to an "Ae. aegypti-free" area. In addition, we found significantly higher anti-SGE IgG concentrations in DENV positive patients with some difference in exposure to mosquito bites among DENV serotypes. We conclude that the concentration of IgG antibodies against SGE is an accurate indicator of risk of dengue virus transmission and disease presence.