RESUMO
BACKGROUND: In the present report we analyse the structural and functional features of sperm from a patient with severe asthenoteratozoospermia and failure of cleavage after ICSI. METHODS: Sperm were studied by phase contrast and transmission electron microscopy and microinjected into bovine oocytes to examine aster formation using antibodies against acetylated alpha- and beta-tubulins. RESULTS: Acephalic sperm, headless tails and abnormal alignments of the head-tail junction were observed. Flagella evidenced the features of dysplasia of the fibrous sheath. Bovine oocytes injected with patient's sperm showed male and female pronuclei but a faulty development of microtubules from the sperm-derived centrosome. The first ICSI attempt using conventional sperm selection methods resulted in fertilized two pronuclei zygotes, but no syngamy or cleavage. Three more ICSI attempts were performed, carefully avoiding sperm with obvious anomalies of the connecting piece. Fertilization and cleavage took place in all cycles, and in two of them positive betahCG plasma levels were detected but preclinical abortions ensued. CONCLUSIONS: We propose that the alterations in the head-tail junction and attachment, responsible for the observed sperm phenotype, result from centriolar dysfunctions that cause insufficient sperm aster formation, lack of syngamy and cleavage or defective embryos leading to early abortions.
Assuntos
Centríolos/fisiologia , Centríolos/ultraestrutura , Fase de Clivagem do Zigoto/fisiologia , Interações Espermatozoide-Óvulo/fisiologia , Espermatozoides/patologia , Espermatozoides/fisiologia , Adulto , Animais , Bovinos , Feminino , Humanos , Masculino , Microscopia Eletrônica , Injeções de Esperma IntracitoplásmicasRESUMO
PURPOSE: To analyze the distribution of a tubulins and acetylated alpha tubulins and the chromatin configuration in abnormally fertilized zygotes from a patient with a multifollicular ovarian response after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). METHODS: Immunofluorescence and phase contrast microscopy was performed in abnormally fertilized zygotes. RESULTS: After phase contrast microscopy analysis, immunofluorescence staining was performed in 20 oocytes that developed > or = 3 pronuclei (PN) and karyomeres after IVF-ICSI. Around 80% of the abnormal zygotes from IVF were the consequence of monospermic fertilizations. Retention of the second polar body (PB) and the presumptive split of > or =1 PN within the cytoplasm were the main events present in most oocytes after IVF-ICSI. CONCLUSIONS: Fluorescence labeling of selected sperm and oocyte components affords a unique view of abnormal fertilized zygotes. Surprisingly, anomalies detected after IVF-ICSI showed similar etiologies in this special group of zygotes.
Assuntos
Citoesqueleto/ultraestrutura , Zigoto/patologia , Acetilação , Adulto , Cromatina/ultraestrutura , Criopreservação , Citoesqueleto/química , Transferência Embrionária , Feminino , Fertilização in vitro , Humanos , Recém-Nascido , Masculino , Meiose , Microscopia de Fluorescência , Microscopia de Contraste de Fase , Síndrome de Hiperestimulação Ovariana/etiologia , Indução da Ovulação/efeitos adversos , Gravidez , Processamento de Proteína Pós-Traducional , Injeções de Esperma Intracitoplásmicas , Cauda do Espermatozoide/química , Cauda do Espermatozoide/ultraestrutura , Espermatozoides , Tubulina (Proteína)/análise , Tubulina (Proteína)/química , Zigoto/químicaRESUMO
PURPOSE: To compare the efficiency of transvaginal ultrasound-guided functional ovarian cyst aspiration, with conservative management, in the outcome of patients undergoing assisted reproductive technique (ART) (in vitro fertilization or intracytoplasmic sperm injection). These cysts were identified before ovarian stimulation begun and after administration of a midluteal GnRH agonist. METHODS: Fifty nine patients undergoing ART from January 1, 1997 to February 28, 1999, who developed functional ovarian cysts were included. Aspirations of these cysts (n = 14) versus conservative management (observation) (n = 45) were compared. Total number of ovarian follicles developed, number of oocytes retrieved, estradiol levels on the day of human chorionic gonadotropin, fertilization rate, number of good quality embryos transferred, implantation, and clinical pregnancy rate per cycle were evaluated. RESULTS: No statistical differences were observed between the two groups in any of the selected parameters. CONCLUSIONS: Cyst aspiration and conservative management showed similar implantation and pregnancy rates, in patients who develop functional ovarian cysts after pituitary down-regulation following luteal phase gonadotropin-releasing hormone agonist administration. Prospective studies are needed to confirm this trend.
Assuntos
Fertilização in vitro/métodos , Cistos Ovarianos/cirurgia , Cistos Ovarianos/terapia , Ovário/patologia , Indução da Ovulação , Adulto , Gonadotropina Coriônica/sangue , Estradiol/sangue , Feminino , Hormônio Liberador de Gonadotropina/efeitos adversos , Hormônio Liberador de Gonadotropina/análogos & derivados , Hormônio Liberador de Gonadotropina/farmacologia , Humanos , Hormônio Luteinizante/sangue , Oócitos/efeitos dos fármacos , Oócitos/metabolismo , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/patologia , Folículo Ovariano/cirurgia , Ovário/efeitos dos fármacos , Ovário/cirurgia , Injeções de Esperma IntracitoplásmicasRESUMO
Comparar los resultados en técnicas de reproducción asistida (ART) empleando dos vias diferentes de administración de análogos de GnRH (diario vs. depósito9(AU)
Assuntos
Gravidez , Humanos , Feminino , Técnicas Reprodutivas/estatística & dados numéricos , Hormônios Liberadores de Hormônios Hipofisários/análise , ArgentinaRESUMO
Comparar los resultados en técnicas de reproducción asistida (ART) empleando dos vias diferentes de administración de análogos de GnRH (diario vs. depósito9