RESUMO

The genes phaR, phaP, and phaF, encoding putative regulatory proteins, were found in the poly (3-hydroxybutyrate) (PHB) gene cluster of the free nitrogen-fixing bacteria Azotobacter sp. FA8. These genes were flanked by the insertion sequence ISAzsp1, belonging to the IS3 family, and a region highly homologous to insertion sequences of the IS630 family. These are the first site-specific recombination elements to be described in association with genes involved in the metabolism of polyhydroxyalkanoates (PHAs). A possible role for ISs in the assembly of pha genes is presented.

Assuntos

Azotobacter vinelandii/genética , Elementos de DNA Transponíveis/genética , Regulação Bacteriana da Expressão Gênica/genética , Genes Bacterianos , Fixação de Nitrogênio/genética , Ácido 3-Hidroxibutírico , Escherichia coli/genética , Família Multigênica/genética , Recombinação Genética , Análise de Sequência de DNA