RESUMO
The authors present observations on 20 cases of sporotrichosis, 12 in dogs and 8 in cats, during 18 months of observation. They conclude: 1) In cats and dogs, sporotrichosis is much more frequent than literature indications. 2) Laboratory diagnosis can"t present troubles, since the agent is relatively abundant in the lesions. 3) Evolution of the disease is very slow and it seems not to affect the general conditions of the patients, since it limits it"s action to the cutaneous tissue. 4) Transmission by direct contact seems to be very difficult.
O artigo apresenta resumo em inglês.
RESUMO
Aspergillosis caused by Aspergillus fumigatus was observed in juriti (Leptotilla rufaxilla) and in cacatua (Cacatua moluccensis). These birds were found dead on their cages at the Parque Zoológico, São Paulo Brasil. Necroscopic examination revealed nodular ball shapped formations distributed along the pulmonar parenchyma, air sacks and mesenteric serosa. Pure cultures of Aspergillus fumigatus were obtained on Sabourauds dextrose ágar in both cases, and suspensions of these cultures caused death in rabbits, guinea pigs and pigeons when give by the intra-venous route. The fungs was reisolated from these animals. Addition to water and meal of Lugols solutions was recommended, after thorough desinfection of the cages to prevent spread of infection to susceptibbles. No further cases were observed. The birds refered in this work were not included yet in the list of susceptibles to Aspergillosis in brazilian scientific literature.
O artigo apresenta resumo em inglês.
RESUMO
According to the results of Rajulu & col. (1960) about bacteriphage relations between strains of staphylococci isolated from dogs and men, the A. A. reviewed their data and found that material from cases of furunculosis which they have tested, were many limes related to students, teachers or workers that were in frequent contact with dogs. The cultures from swabs of skin and nostrils of 113 dogs and 42 students revealed that all dogs were carriers of pathogenic staphylococci, on the skin, nostrils or both. Out of 42 students, 10 were carriers. The strains isolated from dogs were all of the albus type and alpha, beta or alpha-beta hemolysin producers. The strains isolated from students were all of the aureus type and only alpha hemolysin producers. All of the strains fermented dextrose but there was no uniformity as to manitol and gelatin. During the work one of the A. A. presented an apicitis of tooth, from which it was obtained, in pure culture, an albus type betahemolytic an coagulase-positive staphylococus (Phage type 29). None of the strains of canine origin could be phage typed utilizing human or bovine series of phages.
O artigo apresenta resumo em inglês.
RESUMO
The method proposed by Robinow & Murray (Scanga, 1959), to demonstrate the cell wall of bacteria is quite seletive for Gram positives but, when applied to Gram negatives, all the cell becomes evenly stained. The method proposed by Knaysi (1941) althought offering a better contrast is of very delicate performance, to be practical. In this paper, the AA. present a new staining method in order to offer a good contrast between the cell wall and cytoplasmic material. Essentially it consists in turning citoplasmic material unstainable by treatment of the cells with a acid mordent. The subsequent stainning with malachite green or Victoria blue gives a very good contrast. The method can be applied either to Gram positive or Gram negative bacteria.
O artigo apresenta resumo em inglês.
RESUMO
The method proposed by Robinow & Murray (Scanga, 1959), to demonstrate the cell wall of bacteria is quite seletive for Gram positives but, when applied to Gram negatives, all the cell becomes evenly stained. The method proposed by Knaysi (1941) althought offering a better contrast is of very delicate performance, to be practical. In this paper, the AA. present a new staining method in order to offer a good contrast between the cell wall and cytoplasmic material. Essentially it consists in turning citoplasmic material unstainable by treatment of the cells with a acid mordent. The subsequent stainning with malachite green or Victoria blue gives a very good contrast. The method can be applied either to Gram positive or Gram negative bacteria.
O artigo apresenta resumo em inglês.
RESUMO
According to the results of Rajulu & col. (1960) about bacteriphage relations between strains of staphylococci isolated from dogs and men, the A. A. reviewed their data and found that material from cases of furunculosis which they have tested, were many limes related to students, teachers or workers that were in frequent contact with dogs. The cultures from swabs of skin and nostrils of 113 dogs and 42 students revealed that all dogs were carriers of pathogenic staphylococci, on the skin, nostrils or both. Out of 42 students, 10 were carriers. The strains isolated from dogs were all of the albus type and alpha, beta or alpha-beta hemolysin producers. The strains isolated from students were all of the aureus type and only alpha hemolysin producers. All of the strains fermented dextrose but there was no uniformity as to manitol and gelatin. During the work one of the A. A. presented an apicitis of tooth, from which it was obtained, in pure culture, an albus type betahemolytic an coagulase-positive staphylococus (Phage type 29). None of the strains of canine origin could be phage typed utilizing human or bovine series of phages.
O artigo apresenta resumo em inglês.
RESUMO
Aspergillosis caused by Aspergillus fumigatus was observed in juriti (Leptotilla rufaxilla) and in cacatua (Cacatua moluccensis). These birds were found dead on their cages at the Parque Zoológico, São Paulo Brasil. Necroscopic examination revealed nodular ball shapped formations distributed along the pulmonar parenchyma, air sacks and mesenteric serosa. Pure cultures of Aspergillus fumigatus were obtained on Sabourauds dextrose ágar in both cases, and suspensions of these cultures caused death in rabbits, guinea pigs and pigeons when give by the intra-venous route. The fungs was reisolated from these animals. Addition to water and meal of Lugols solutions was recommended, after thorough desinfection of the cages to prevent spread of infection to susceptibbles. No further cases were observed. The birds refered in this work were not included yet in the list of susceptibles to Aspergillosis in brazilian scientific literature.
O artigo apresenta resumo em inglês.
RESUMO
The authors present observations on 20 cases of sporotrichosis, 12 in dogs and 8 in cats, during 18 months of observation. They conclude: 1) In cats and dogs, sporotrichosis is much more frequent than literature indications. 2) Laboratory diagnosis can"t present troubles, since the agent is relatively abundant in the lesions. 3) Evolution of the disease is very slow and it seems not to affect the general conditions of the patients, since it limits it"s action to the cutaneous tissue. 4) Transmission by direct contact seems to be very difficult.
O artigo apresenta resumo em inglês.
RESUMO
For the first time in Brazil Sporothricosis is described in dogs. In the two cases studied the disease was characterized by the appearance of cutaneous nodes of different consistences and sometimes ulcerous, covered with dark scabs. Fistulous openings complete the lesion and purulent discharge could be observed as well as involvement of the drainage limph nodes. In the contents of the nodes, in the purulent exudates of the fistulae and the scrapings of the ulcers, there were many parasites, in characteristics "cigar shape". Cultures were obtained easily at room temperature and at 37 C. The disease followed a chronic course and no marked constitucional symptoms were noted. In one of the cases the treatment with Griseofulvina was not successfull but the ministration of potassium iodine resulted in a quick but false recovery, because after some months there was a recrudescency.
Os autores apresentam dois casos de esporotricose em cães, micose ainda não assinalada nessa espécie, em S. Paulo. O processo, em ambos os casos, teve evolução crônica, não afetou aparentemente o estado geral dos animais e caracterizou-se por lesões cutâneas de caráter úlcero-gomoso em diferentes graus de desenvolvimento, configuradas por formações nodulares de consistência diversa, áreas ulceradas e trajetos fistulosos dos quais vazava secreção purulenta. Havia reação linfática bem evidente acusada pelo pronunciado aumento de volume dos gânglios regionais. Em esfregaços irreparados com o pus das fístulas ou cor. raspados das úlceras, corados pelo Gram, o parasita foi facilmente reconhecido pela sua "forma em charuto". Foram obtidas culturas típicas em Sabouraud maltosado e glicosado, em temperatura ambiente e a 37C. Em um dos casos, o tratamento com griseofulvina "(Grifulvin)" não teve êxito. A administração de iodeto de potássio reverteu em rápida melhora, aparentando cura, porém houve recidiva após alguns meses.
RESUMO
For the first time in Brazil Sporothricosis is described in dogs. In the two cases studied the disease was characterized by the appearance of cutaneous nodes of different consistences and sometimes ulcerous, covered with dark scabs. Fistulous openings complete the lesion and purulent discharge could be observed as well as involvement of the drainage limph nodes. In the contents of the nodes, in the purulent exudates of the fistulae and the scrapings of the ulcers, there were many parasites, in characteristics "cigar shape". Cultures were obtained easily at room temperature and at 37 C. The disease followed a chronic course and no marked constitucional symptoms were noted. In one of the cases the treatment with Griseofulvina was not successfull but the ministration of potassium iodine resulted in a quick but false recovery, because after some months there was a recrudescency.
Os autores apresentam dois casos de esporotricose em cães, micose ainda não assinalada nessa espécie, em S. Paulo. O processo, em ambos os casos, teve evolução crônica, não afetou aparentemente o estado geral dos animais e caracterizou-se por lesões cutâneas de caráter úlcero-gomoso em diferentes graus de desenvolvimento, configuradas por formações nodulares de consistência diversa, áreas ulceradas e trajetos fistulosos dos quais vazava secreção purulenta. Havia reação linfática bem evidente acusada pelo pronunciado aumento de volume dos gânglios regionais. Em esfregaços irreparados com o pus das fístulas ou cor. raspados das úlceras, corados pelo Gram, o parasita foi facilmente reconhecido pela sua "forma em charuto". Foram obtidas culturas típicas em Sabouraud maltosado e glicosado, em temperatura ambiente e a 37C. Em um dos casos, o tratamento com griseofulvina "(Grifulvin)" não teve êxito. A administração de iodeto de potássio reverteu em rápida melhora, aparentando cura, porém houve recidiva após alguns meses.
RESUMO
In this paper it is presented the results of bacteriological examination of swabs obtained from the os uteris or from the uterus of thorough bred mares, distributed in various race-horse farms of the State of São Paulo Brazil. The samples were classified according to the origin from infected mares (infected group) and from non infected, healthy (non treated) and cured mares (control group). From the infected group we get 194 samples which, on test, gave the following results: Streptococcus zooepicdemicus - 46,9%; Staphylococcus aureus (albus tvpe) - 12,8%; Escherichia coli 11.8%; coliforms - 14,9%; Pseudomonas aeruginosa - 8,2%; Corynebacterium pyogenes - 0,5% and Proteus vulgaris - 0,5%. Eight mares treated for a long period of time with antibiotics gave pure cultures of yeasts (superinfections). Many non pathogenic bacteria were also found on cultures, associated with pathogens. From the non infected group we obtained 163 samples with the following resultts: coagulase negative staphylococcus 8,5% sporulated aerobic bacilli of the cereus subtilis group 6,1 %; viridans streptococci 4,9% and diphteroids 2,4%. From the 163 samples, 124 (76,0%) gave no growth at all. The germs found by the A. A. and isolated from the infected group were considered as the etiological agents of the genital infections because they lack in the samples obtained from animals treat
O artigo apresenta resumo em inglês.
RESUMO
In this paper it is presented the results of bacteriological examination of swabs obtained from the os uteris or from the uterus of thorough bred mares, distributed in various race-horse farms of the State of São Paulo Brazil. The samples were classified according to the origin from infected mares (infected group) and from non infected, healthy (non treated) and cured mares (control group). From the infected group we get 194 samples which, on test, gave the following results: Streptococcus zooepicdemicus - 46,9%; Staphylococcus aureus (albus tvpe) - 12,8%; Escherichia coli 11.8%; coliforms - 14,9%; Pseudomonas aeruginosa - 8,2%; Corynebacterium pyogenes - 0,5% and Proteus vulgaris - 0,5%. Eight mares treated for a long period of time with antibiotics gave pure cultures of yeasts (superinfections). Many non pathogenic bacteria were also found on cultures, associated with pathogens. From the non infected group we obtained 163 samples with the following resultts: coagulase negative staphylococcus 8,5% sporulated aerobic bacilli of the cereus subtilis group 6,1 %; viridans streptococci 4,9% and diphteroids 2,4%. From the 163 samples, 124 (76,0%) gave no growth at all. The germs found by the A. A. and isolated from the infected group were considered as the etiological agents of the genital infections because they lack in the samples obtained from animals treat
O artigo apresenta resumo em inglês.
RESUMO
In this additional paper, the Author has obtained induced resistant strains of Streptococcus equi through serial transfers in penicillin-added broth. These resistant strains exhibited morphological and cultural alterations, having lost their hemolytic power and virulence for mice. After 55 transfers, the degree of resistance attained a minimum of 9.2 times and a maximum of 100.0 times, comparatively with the sensible parents. The resistant strains were then sub-cultivated in penicillin free broth and all of them have re-acquired their original properties, including the virulence for mice and the sensitivity to penicillin. Considering the hypothesis that these phenomena could be established in vivo , during a survey of strangles, it is suggested that the occurrence of penicillin-resistant variants of St. equi would not have any epidemiological significance because they would not be able to produce the disease. Even considering that these strains could be disseminated by contagium they would not represent any problem because the resistant phase is transitory and reversible.
O artigo apresenta resumo em inglês.
RESUMO
The Author investigated the occurrence of naturally antibiotic- resistant strains of Streptococcus equi. A lot of 31 strains was isolated from nostrils or suppured lymph glands of race horses, along a survey of strangles in Jockey Club Paulistano São Paulo Brasil. Between them, there were 31 animals with active strangles, 93 clinically healed and 101 healthy adult horses. All strains identified as Streptococcus equi were tested by the disc-plate method against penicillin, streptomycin, aureomycin, terramycin and chloromycetin in variable concentrations of the antibiotics. Seven out of all showed resistance against 0,5 unit of penicillin and two others against 1 meg of streptomycin. Repeating the test, now by the dilution test tube method, the same strains did not confirm those fastness properties. The results suggest two possibilities: a) the disc-plate method was not so accured as the dilution test tube method; b) the strains were really resistant when recently isolated but along subcultures they have lost their drug-fastness. As a matter of fact, this condition was confirmed in another investigation, when resistant strains were obtained by successive transfers in broth with penicillin. The actual data suggest that naturally antibiotic-resistant strains of Streptococcus equi do not occur, exception not so clear being made for some strains in relation to penicillin.
O artigo apresenta resumo em inglês.
RESUMO
The Author investigated the occurrence of naturally antibiotic- resistant strains of Streptococcus equi. A lot of 31 strains was isolated from nostrils or suppured lymph glands of race horses, along a survey of strangles in Jockey Club Paulistano São Paulo Brasil. Between them, there were 31 animals with active strangles, 93 clinically healed and 101 healthy adult horses. All strains identified as Streptococcus equi were tested by the disc-plate method against penicillin, streptomycin, aureomycin, terramycin and chloromycetin in variable concentrations of the antibiotics. Seven out of all showed resistance against 0,5 unit of penicillin and two others against 1 meg of streptomycin. Repeating the test, now by the dilution test tube method, the same strains did not confirm those fastness properties. The results suggest two possibilities: a) the disc-plate method was not so accured as the dilution test tube method; b) the strains were really resistant when recently isolated but along subcultures they have lost their drug-fastness. As a matter of fact, this condition was confirmed in another investigation, when resistant strains were obtained by successive transfers in broth with penicillin. The actual data suggest that naturally antibiotic-resistant strains of Streptococcus equi do not occur, exception not so clear being made for some strains in relation to penicillin.
O artigo apresenta resumo em inglês.
RESUMO
In this additional paper, the Author has obtained induced resistant strains of Streptococcus equi through serial transfers in penicillin-added broth. These resistant strains exhibited morphological and cultural alterations, having lost their hemolytic power and virulence for mice. After 55 transfers, the degree of resistance attained a minimum of 9.2 times and a maximum of 100.0 times, comparatively with the sensible parents. The resistant strains were then sub-cultivated in penicillin free broth and all of them have re-acquired their original properties, including the virulence for mice and the sensitivity to penicillin. Considering the hypothesis that these phenomena could be established in vivo , during a survey of strangles, it is suggested that the occurrence of penicillin-resistant variants of St. equi would not have any epidemiological significance because they would not be able to produce the disease. Even considering that these strains could be disseminated by contagium they would not represent any problem because the resistant phase is transitory and reversible.
O artigo apresenta resumo em inglês.
RESUMO
Leptospirosis as cause of bovine abortion was seen forthe first time in São Paulo (Brazil). The use of a polivalentantigen for rapid aglutination test, has suggested the presenceof the disease, thus 18 out of 150 serum samples gavepositive titres. Amongst 18 sera 16 reacted positively againstL. pomona antigen. L. pomona could be isolated from anaborted fetus and it was possible to transmit the infectionto calves and guinea-pigs.
O artigo não apresenta resumo.
RESUMO
From two newborn foals, one affected with poliarthritis and other with clinical symptoms of "sleeping foal Shigella equirulis could be isolated. It is the first time that this germ is identified in Brazil.
O artigo apresenta resumo em inglês.
RESUMO
From two newborn foals, one affected with poliarthritis and other with clinical symptoms of "sleeping foal Shigella equirulis could be isolated. It is the first time that this germ is identified in Brazil.
O artigo apresenta resumo em inglês.
RESUMO
Leptospirosis as cause of bovine abortion was seen forthe first time in São Paulo (Brazil). The use of a polivalentantigen for rapid aglutination test, has suggested the presenceof the disease, thus 18 out of 150 serum samples gavepositive titres. Amongst 18 sera 16 reacted positively againstL. pomona antigen. L. pomona could be isolated from anaborted fetus and it was possible to transmit the infectionto calves and guinea-pigs.
O artigo não apresenta resumo.