RESUMO
Background: Aspergillus fumigatus is considered the major agent of mycotic diseases in birds, affecting mainly the respiratory tract. It is a disease of economic importance in the poultry industry, however it is not a zoonotic or contagious disease. Aspergillus spp. are an environment residents. Infection usually occurs by inhalation of conidia released by molds, which come off the diet or specific ingredients, the nest and contamination of eggs during incubation. The objective of this study is to relate the macro and microscopic diagnosis of aspergillosis in poultry. Case: In an intensive farming of poultry (Gallus gallus), it was observed mortality rate exceeding 20%, hoarseness and difficulty breathing in males of approximately two weeks of age. The batch was treated with Terramycin ® (oxytetracyclinehydrochloride) in the first week and Trissulfin® (sulfamethoxazole, trimethoprim and bromhexine hydrochloride) in the second week. Birds were sent for analysis at the Laboratório Central de Diagnóstico de Patologias Aviárias (LCDPA) of the Universidade Federal de Santa Maria (UFSM). Necropsy was performed in three affected birds and pulmonary aspergillosis was suspected due to local pulmonary and disseminated injuries in the coelomic cavity, associated to the clinical signs. In birds assessed by necropsy examination, it was common the visualization of nodules in the internal cavity and lungs, caseous masses in the air sacs, little pigmentation on the feet and beaks and fragile bones. Portions of lung and granulomas were sent for isolation and identification of fungi in the Laboratório de Pesquisas Micológicas (LAPEMI)-UFSM and histopathological analysis in the Laboratório de Patologia Veterinária (LPV)-UFSM, where the standard protocols for each exam were used. The result of mycological examination showed Aspergillus fumigatus as the agent. The histopathological lesions observed in the lung were consistent with aspergillosis, characterized by multifocal granulomas associated with intra-lesional dichotomously branched fungal hyphae, morphologically compatible with Aspergillus sp. Discussion: The occurrence of aspergillosis depends on the dose of inhaled conidia of the fungus and the susceptibility of the host, which occurs in birds in the first weeks of age, making it more resistant to infection in adults. The history of signs consisting of respiratory distress associated with stressful situations or the recent lack of response to antibiotics may provide support to the clinical diagnosis of aspergillosis. The isolation and identification of the fungus comprises the best method to confirm the disease agent. Histopathology provides an important contribution to the morphological diagnosis of the lesion and the fungus. The treatment of aspergillosis in poultry production is difficult and uneconomical, so that all attention is focused on prevention and control in poultry houses and hatcheries. Eggs for incubation must be cleaned and disinfected, dirty and cracked eggs should not be incubated. Care should be strict with hygiene in the hatchery. Once detected the source, it should be eliminated, and the implementation of antifungal agents according to the location of contamination and the substrate. The major difficulty for the prevention and control of aspergillosis is because these fungi can be present at all stages of poultry production.
Assuntos
Animais , Masculino , Aspergilose/diagnóstico , Aspergilose/prevenção & controle , Aspergillus fumigatus/patogenicidade , Galinhas/imunologiaRESUMO
Testou-se a infecção de Trypanosoma evansi pela via oral em ratos e camundongos, através de sangue contaminado de ambas as espécies. Dez ratos e dez camundongos foram alocados em quatro grupos iguais A e B (ratos), C e D (camundongos). Os grupos A e C receberam sangue contaminado de um rato e o grupo B e D de um camundongo, através de uma sonda. O volume de sangue administrado foi de 0,2ml, o qual apresentava uma concentração de 10(7) tripanossomas ml-1. Os animais foram mantidos em temperatura e umidade constantes (25°C e 80 por cento UR), sendo realizados esfregaços sanguíneos diários para identificar o período pré-patente e a evolução do parasita na circulação. Nos grupos A e B, o período pré-patente variou de 19 a 25 dias, e o período entre a detecção dos parasitas e a morte dos animais foi em média de 12,7 dias. Os camundongos do grupo C e D não apresentaram infecção pelo parasita, sendo estes avaliados por 60 dias. Os ratos foram susceptíveis a infecção por T. evansi pela via oral; entretanto, os camundongos não se contaminaram com o protozoário por via digestiva.
In this research, Trypanosoma evansi infection was tested in rats and mice by oral ingestion of contaminated blood. Groups of ten rats and ten mice were disposed in four experimental groups: A and B (rats), C and D (mice). The groups A and C were contaminated by rat-contaminated blood; B and C groups by mouse-contaminated blood. The blood was given using a probe filled with 0.2ml of contaminated blood with 10(7) trypanosomes ml-1. These animals were maintained at constant temperature and humidity (25°C and 80 percent UR). Dairy blood smear were done to identify the prepatent period and evolution of parasite in the circulation. In the A and B groups, the pre latency period varied from 19 to 25 days and the period of parasite detection and animals death was an average of 12.7 days. The C and D groups did not present infection by the parasite even when evaluated for 60 days. In conclusion, the rats had oral infection by T. evansi but this protozoan couldnÆt contaminate the mice by digestive path.