RESUMO
In this study an automatic method for sample treatment and spectrophotometric determination of hydroxyproline in commercial sausages was developed. A flow-batch system that includes the steps of sample hydrolysis and determination of the analyte was designed. The method presents a linear range between 0.60 and 3.60 µg mL(-1) of hydroxyproline. A relative standard deviation of 1.68% was obtained from hydroxyproline standard solution (n=6, 1.20 µg mL(-1)) and the detection limit was 0.12 µg mL(-1). The sample throughput was 1 sample h(-1) while the reference method (AOAC) was carried out in about 17 h. This method employs 16 h of hydrolysis while in the proposed method the hydrolysis time was 15 min. For this purpose a pressure hydrolysis chamber with a low cost halogen lamp was used. The flow-batch system is simple and allows the use of both chambers (hydrolysis and detection) simultaneously. The obtained results with the flow-batch method are in good agreement with that obtained with the reference method. Moreover, it is good alternative to the quality control of meat products.
Assuntos
Automação Laboratorial/métodos , Hidroxiprolina/análise , Produtos da Carne/análise , Animais , Automação Laboratorial/instrumentação , Calibragem , Bovinos , Hidrólise , Limite de Detecção , Pressão , Controle de Qualidade , Padrões de Referência , Soluções , Espectrofotometria , Suínos , Fatores de TempoRESUMO
El desarrollo del trabajo se basa en el modelo conceptual y el conjunto de variables que propone Mosley para el estudio de la mortalidad en la niñez. Constituye un primer paso para el analisis mas profundo de los determinantes del comportamiento de esa variable demografica en la Republica Argentina. Se presenta una caracterizacion de la provincia de Misiones como marco de referencia para facilitar el analisis y la interpretacion de los resultados obtenidos
Assuntos
Mortalidade InfantilRESUMO
El desarrollo del trabajo se basa en el modelo conceptual y el conjunto de variables que propone Mosley para el estudio de la mortalidad en la niñez. Constituye un primer paso para el analisis mas profundo de los determinantes del comportamiento de esa variable demografica en la Republica Argentina. Se presenta una caracterizacion de la provincia de Misiones como marco de referencia para facilitar el analisis y la interpretacion de los resultados obtenidos