RESUMO
The growth and toxin production of Clostridium argentinense in co-culture with Pseudomonas mendocina was examined in a micro-fermenter without aeration characterizing the association in terms of several growth parameters. The biomass obtained in co-cultures was 4.4 times higher than that in C. argentinense monocultures with total consumption of the carbon source. The pH and Eh attained in co-cultures at later stages of cultivation were suitable for toxin production by C. argentinense. In comparison with C. argentinense monocultures the production of toxin was 17.5 times higher with a specific toxicity of 0.56 LD50 per g of co-culture biomass.
Assuntos
Toxinas Bacterianas/biossíntese , Clostridium/crescimento & desenvolvimento , Clostridium/metabolismo , Pseudomonas/crescimento & desenvolvimento , Pseudomonas/metabolismo , Animais , Meios de Cultura , Glucose/metabolismo , Concentração de Íons de Hidrogênio , CamundongosRESUMO
Five cases of infant botulism which occurred during the period from May 1995 to May 1996 in San Luis, Argentina, are reported. Infant botulism was confirmed in all patients by isolation of Clostridium botulinum type A from stool culture and by the toxin assay. Toxin was found in the serum of one of them. All patients required hospitalization with treatment consisting of supportive especially respiratory and nutritional care. At the time of discharge from the hospital, three patients had a good recovery, although two of them had mild difficulties in sucking or constipation. C. botulinum was not detected in samples of honey which had been given to two of the patients.
Assuntos
Botulismo/diagnóstico , Clostridium botulinum/isolamento & purificação , Argentina/epidemiologia , Toxinas Botulínicas/sangue , Toxinas Botulínicas/isolamento & purificação , Botulismo/sangue , Botulismo/epidemiologia , Feminino , Humanos , Lactente , MasculinoRESUMO
The strain G89HT of Clostridium argentinense obtained by culture selection of the prototype G89 strain producing high titers of type G botulinal toxin was studied. Its cultural, biochemical and toxigenic characteristics and the presence of plasmids were tested. Both strains showed similar physiological features and carried a 83 MDa plasmid. A 170 MDa plasmid was also recognized in the G89HT strain. Notably, this strain was better sporulating and showed a higher toxigenicity than the prototype G89 C. argentinense strain. These two characteristics might permit a long term storage and perhaps yield high antitoxin titres.
Assuntos
Toxinas Botulínicas/metabolismo , Clostridium/metabolismo , Proteínas de Bactérias/análise , Clostridium/crescimento & desenvolvimento , Clostridium/patogenicidade , Meios de Cultura/metabolismo , Testes de Neutralização , Plasmídeos/genética , Plasmídeos/isolamento & purificação , Esporos Bacterianos/genética , Esporos Bacterianos/crescimento & desenvolvimento , Esporos Bacterianos/metabolismo , Tripsina/metabolismo , Virulência/genéticaRESUMO
Se examinaron 177 muestras de miel para la detección de Clostridium botulinum; 68 procedentes del comercio, 95 de apiarios envasados en origen, 8 de apiarios rurales para consumo familiar y 6 de establecimientos fraccionadores en las provincias de San Luis y Mendoza. Utilizando el método de dilución-centrifugación, se detectó C. botulinum tipo A en 2 muestras de apiarios rurales de San Luis (1,1 o/o); en una de ellas se logró la recuperación de la cepa y el recuento de esporas fue de 55/g de miel. Si bien el porcentaje de positividad fue inferior al de otros países, se recomienda no suministrar miel a niños menores de 1 año como prevención de botulismo del lactante (AU)
Assuntos
Humanos , Lactente , Clostridium botulinum/isolamento & purificação , Botulismo/diagnóstico , Esporos Bacterianos/isolamento & purificação , Mel/análise , Toxinas Botulínicas/isolamento & purificaçãoRESUMO
Se examinaron 177 muestras de miel para la detección de Clostridium botulinum; 68 procedentes del comercio, 95 de apiarios envasados en origen, 8 de apiarios rurales para consumo familiar y 6 de establecimientos fraccionadores en las provincias de San Luis y Mendoza. Utilizando el método de dilución-centrifugación, se detectó C. botulinum tipo A en 2 muestras de apiarios rurales de San Luis (1,1 o/o); en una de ellas se logró la recuperación de la cepa y el recuento de esporas fue de 55/g de miel. Si bien el porcentaje de positividad fue inferior al de otros países, se recomienda no suministrar miel a niños menores de 1 año como prevención de botulismo del lactante
Assuntos
Humanos , Lactente , Botulismo/diagnóstico , Clostridium botulinum/isolamento & purificação , Mel/análise , Esporos Bacterianos/isolamento & purificação , Toxinas Botulínicas/isolamento & purificaçãoRESUMO
A total of 177 honey samples were examined for Clostridium botulinum, 68 of which were from commercial origin, 8 from small rural producers for family consumption, and the remaining 6 from fractionizing centers in Mendoza and San Luis provinces in Argentina. C. botulinum type A was detected in two samples of rural producer origin (1.1%) by the centrifugation-dilution method. The strain was recovered from one of the samples, obtaining a spore count of 55/g of honey. Even though the positive percentage was lower than that found in other countries, honey consumption by children under one year old should be avoided in order to prevent infant botulism.
Assuntos
Clostridium botulinum/isolamento & purificação , Contaminação de Alimentos , Mel/microbiologia , Argentina , Botulismo/etiologia , Botulismo/prevenção & controle , Humanos , Lactente , Esporos Bacterianos/isolamento & purificaçãoRESUMO
The bacteriological analysis and, particularly, the detection of Clostridium botulinum spores from 42 honey samples collected in apiaries of the province of San Luis as well as neighbouring areas of La Pampa, Córdoba and Mendoza, were carried out. Samples were processed by the dilution and centrifugation procedures. For spores detection, culture of the pellets were performed in 2 tubes with cooked meat medium (MCC), one of them warmed up to 80 degrees C for 15 min, and both incubated at 30 degrees C during 7 days. Mice were used to search for toxin in the supernatant. Sediments were also searched for anaerobic bacteria detection in yolk agar plates and in nutritive agar plates for the aerobics. Botulinum toxin type A production was found in one of the MCC cultures. No anaerobic bacteria were isolated. All samples contained Bacillus spp.; 21.4% of the strains, were tentatively classified as B. alvei. A working model for the bacteriological analysis of honey and guides that could be enclosed in publications of official institutions (Figure 1) is proposed.
Assuntos
Clostridium botulinum/isolamento & purificação , Mel/microbiologia , Técnicas Bacteriológicas , Esporos Bacterianos/isolamento & purificaçãoRESUMO
The bacteriological analysis and, particularly, the detection of Clostridium botulinum spores from 42 honey samples collected in apiaries of the province of San Luis as well as neighbouring areas of La Pampa, Córdoba and Mendoza, were carried out. Samples were processed by the dilution and centrifugation procedures. For spores detection, culture of the pellets were performed in 2 tubes with cooked meat medium (MCC), one of them warmed up to 80 degrees C for 15 min, and both incubated at 30 degrees C during 7 days. Mice were used to search for toxin in the supernatant. Sediments were also searched for anaerobic bacteria detection in yolk agar plates and in nutritive agar plates for the aerobics. Botulinum toxin type A production was found in one of the MCC cultures. No anaerobic bacteria were isolated. All samples contained Bacillus spp.; 21.4
of the strains, were tentatively classified as B. alvei. A working model for the bacteriological analysis of honey and guides that could be enclosed in publications of official institutions (Figure 1) is proposed.
RESUMO
The bacteriological analysis and, particularly, the detection of Clostridium botulinum spores from 42 honey samples collected in apiaries of the province of San Luis as well as neighbouring areas of La Pampa, Córdoba and Mendoza, were carried out. Samples were processed by the dilution and centrifugation procedures. For spores detection, culture of the pellets were performed in 2 tubes with cooked meat medium (MCC), one of them warmed up to 80 degrees C for 15 min, and both incubated at 30 degrees C during 7 days. Mice were used to search for toxin in the supernatant. Sediments were also searched for anaerobic bacteria detection in yolk agar plates and in nutritive agar plates for the aerobics. Botulinum toxin type A production was found in one of the MCC cultures. No anaerobic bacteria were isolated. All samples contained Bacillus spp.; 21.4
of the strains, were tentatively classified as B. alvei. A working model for the bacteriological analysis of honey and guides that could be enclosed in publications of official institutions (Figure 1) is proposed.