RESUMO

The complete cDNA sequence corresponding to the genomic RNA of a South American strain of potato virus X (PVXc) is reported. The sequence (6432 nucleotides) contains five open reading frames coding for polypeptides with molecular weights of 165.3, 24.3, 12.3, 7.6 and 25.0 and displays an overall homology of 77.4% with those previously reported for two European isolates. Comparison of amino acid sequences shows an average homology of 87%. Two major domains of variability, located between amino acids 476-615 of ORF 1 and 64-100 of ORF 5, are identified. Sequence similarities between RNA stretches lying upstream of ORFs 2, 4 and 5, and at the 3'-non coding regions of PVX and other plus-strand RNA viruses are described.

Assuntos

Genes Virais , Vírus de Plantas/genética , RNA Viral , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA Viral , Europa (Continente) , Dados de Sequência Molecular , RNA Viral/isolamento & purificação , Homologia de Sequência do Ácido Nucleico , Solanum tuberosum/microbiologia , América do Sul