RESUMO
BACKGROUND: Plants of the Myrcia genus have been widely used in folk medicine to treat various diseases, including cancer. Myrcia splendens species has a diverse chemical constitution, but the biological activities of its essential oil have not been well investigated. In this study to out the chemistry characterization of essential oil (EO) from the leaves of the species M. splendens from Brazil and evaluate cytotoxic effect in A549 lung cancer cells. METHODS: M. splendens EO was obtained by hydrodistillation and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). EO was isolated and evaluated for cellular viability in tumor cell lines by MTT assay. The evaluation of the formation of clones and the migratory capacity of the A549 cells treated with EO was done by the clonogenic assay and the wound healing assay. Morphological changes were observed in A549 cells by fluorescence using Phalloidin/FITC and DAPI. RESULTS: 22 compounds were identified in the chemical analysis of EO, corresponding to 88% of the sample. Major compounds were the sesquiterpenic hydrocarbons bicyclogermacrene (15.4%), germacrene D (8.9%) and E-caryophyllene (10.1%). The biological analysis of the EO showed high cytotoxic activity with an IC50 below 20 µg/ml in the THP-1, A549 and B16-F10 tumor cells. The treatment with EO reduced colony formation and inhibited the migratory capacity of A549 cells. Furthermore, apoptotic morphological changes in the nucleus and cytoplasm of A549 cells was observed after of treatment with EO. CONCLUSION: The findings of this study suggest that the M. splendens EO has cytotoxic compounds for the A549 lung cancer cells. Treatment with the EO decreased the colony formation and reduced the ability of lung cancer cells to migrate. Future studies may be used to isolate compounds from the EO for the study of lung cancer.
Assuntos
Antineoplásicos , Neoplasias Pulmonares , Myrtaceae , Óleos Voláteis , Humanos , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Células A549 , Cromatografia Gasosa-Espectrometria de Massas , Antineoplásicos/farmacologia , Neoplasias Pulmonares/tratamento farmacológicoRESUMO
ABSTRACT Leishmania infantum is an etiologic agent of visceral leishmaniasis. This disease is a neglected disease that can be fatal if not treated and additionally, the few therapeutic option present several drawbacks, including difficult route of administration and toxicity, which turn the search for new therapeutic alternatives necessary. Herein, we evaluated the leishmanicidal in vitro activity of the solanum extract from Solanum lycocarpum A. St.-Hil., Solanaceae, and the isolated alkaloids solasodine, solamargine and solasonine against promastigotes and intracellular amastigotes of L. infantum. Solasodine (IC50-pro = 4.7 µg/ml; IC50-ama = 10.8 µg/ml) and solamargine (IC50-pro = 8.1 µg/ml; IC50-ama = 3.0 µg/ml) exhibited interesting leishmanicidal ativity. Solasonine was approximately four-times (Selective Index 3.7) more selective to the parasite than to the host cells. This data suggest that solasonine might be considered as a potential drug candidate for leishmaniasis treatment.
RESUMO
The composition of the essential oil (EO) from leaves of Vernonia polyanthes and the evaluation of its leishmanicidal potential are reported here for the first time. The oil obtained by hydrodistillation was analysed by combination of GC and GC/MS. Thirty-five compounds were identified, representing 91.8% of the oil composition. The oil consists primarily of monoterpenes (37.1%), sesquiterpenes (26.3%) and oxygenated sesquiterpenes (23.9%), myrcene (34.3%), zerumbone (15.8%), bicyclogermacrene (8.9%), α-humulene (4.8%) and germacrene D (4.3%) being the major constituents. Activity against Leishmania infantum was determined using the tetrazolium dye (MTT) colorimetric method. The oil, as well as zerumbone, one of its major constituents, showed significant leishmanicidal activity, with IC50 values of 19.4 and 9.0 µg/ml, respectively. Cytotoxicity in macrophages cells was evaluated using the MTT colorimetric assay. The EO showed the CC50 < 10 µg/ml to macrophages cells.
Assuntos
Antiprotozoários/isolamento & purificação , Leishmania infantum/efeitos dos fármacos , Óleos Voláteis/química , Vernonia/química , Animais , Antiprotozoários/química , Antiprotozoários/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Macrófagos/efeitos dos fármacos , Monoterpenos/análise , Óleos Voláteis/uso terapêutico , Folhas de Planta/química , Sesquiterpenos/análiseRESUMO
The essential oil obtained by hydrodistillation from dry leaves of Capsicodendron dinisii (Schwancke) was analyzed using gas chromatography (GC) and gas chromatography/mass (GC/MS) spectrometry. According to GC and GC/MS analysis, the essential oil is mostly composed of sesquiterpene hydrocarbons (69.7 percent) and oxygenated sesquiterpenes (22.6 percent). Bicyclogermacrene (30.8 percent), E-caryophyllene (17.2 percent), spathulenol (13.5 percent) and germacrene D (7.6 percent) were the principal components identified in the essential oil.
El aceite esencial obtenido por hidrodestilación de hojas secas de Capsicodendron dinisii (Schwancke) se analizó mediante cromatografía de gases y cromatografía de gases/espectrometría de masas. De acuerdo con los análisis, el aceite esencial contiene un 69,7 por ciento de sesquiterpenos hidrocarbonados y 22,6 por ciento de sesquiterpenos oxigenados. Bicyclogermacreno (30.8 por ciento), E-cariofileno (17.2 por ciento), espathulenol (13.5 por ciento) and germacreno D (7.6 por ciento) fueron los componentes principales identificados en el aceite esencial.
Assuntos
Óleos Voláteis/química , Magnoliopsida/química , Folhas de Planta/química , Sesquiterpenos/análise , Brasil , Cromatografia Gasosa , Cromatografia Gasosa-Espectrometria de MassasRESUMO
Background: The properties of plants with food preservation potential are well known since the antiquity.In recent years, the use of herbs and spices to improve the sensory characteristics and to extendthe shelf-life of foods has been growing. Objectives: To compare oregano (Origanum vulgare L.) and sage(Salvia officinalis L.) as a natural antioxidant in balls of chicken breast and added 0.50% salt. Methods:Samples of chicken meatballs were pre-cooked for 8 minutes in a water bath at 80o C, and packaged inpolyethylene bags with three layers, specific for vacuum cooking and high temperatures. The sampleswere separated into three groups: control (just salt), 0.10% oregano (dry plant) and sage 0.10% (dry plant),stored at -20o C for 144 hours. The tests to verify the formation of hexanal in samples were performedin the period of 0, 48, 96 and 144 hours of refrigerated storage. The determination of hexanal, extractedby micro-extraction (headspace solid phase micro-extraction - HS-SPME), was used as an indicator ofthe lipid oxidation of the samples. The analysis was conducted by a gas chromatograph coupled witha mass detector (GC-MS). The method was evaluated according to the validation parameters such aslinearity, repeatability and detection limit. The extraction was conducted at 70o C using a fiber (DVD/ CAR / PDMS) exposed for 20 minutes in the headspace after 5 minutes of equilibrium between thesample and the headspace. Results: Samples added 0.10% oregano or sage had effective protection in thedevelopment of hexanal, compared to control samples. The development of hexanal was significantlyhigher in the control samples, 34 Êg of hexanal/100 g of sample at the beginning and 280 Êg/100 g sampleat the end of refrigerated storage...