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BACKGROUND: MCs (MCs) have been ascribed to mediating several diseases, including malignant neoplasms. These cells can play a role in angiogenesis, tissue remodeling and immune modulation and favor neoplasm progression. Despite the studies analyzing the contribution of MCs in odontogenic lesions, its biological behavior in ameloblastomas (AMBs) and odontogenic keratocysts (OKCs) remains unclear. This study aims to detect MCs in OKCs and AMBs and clarify the role of MCs in these lesions. MATERIAL AND METHODS: A total of 40 odontogenic lesions were analyzed. This included 20 OKCs and 20 AMBs, 10 being the solid type and the other 10 being the unicystic type of AMB. All cases were histologically reviewed in hematoxylin-eosin. Clinical data, such as age, gender, location, size, radiographic presentation and, histologic patterns were collected from the clinical charts. The Mann-Whitney U test (MWU) was used verify the hypothesis, through inferential statistics. The level of significance used in the statistical test was 0.5%. RESULTS: MCs were observed in 60% of OKCs, and 35% of AMBs. The ratio of MCs observed in OKCs was 0.37, 0.48 in solid AMBs and 0.01 in unicystic AMBs. There was no significant difference between number of MCs in AMBs and OKCs, however, a significant difference was observed between solid and unicystic AMBs (p ≤ 0.01). CONCLUSIONS: MCs may play an important role in the biological behavior of AMBs and OKCs. However, in this study it was not possible to confirm the contribution of MCs in the biological behavior of these lesions and more studies are needed to clarify this relation. Key words:AMB, OKC, MCs, histochemistry, toluidine blue.
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BACKGROUND: To evaluate, in vitro, the potential cytotoxicity of three different dental adhesives systems (Adper Single Bond 2 -SB, Silorane System Adhesive Bond -SSAB and Single Bond Universal -SBU) on cultivated Vero cells after different contact times. MATERIAL AND METHODS: The cells were cultured in a concentration of 2 x 105 cells/mL for 24h and grown to sub-confluent monolayers. VERO cells were exposed to 25µl of conditioned extracts obtained from 24h, 48h and 72h immersion of adhesive samples in culture medium (DMEM), immediately after polymerization. Fresh DMEM was used as negative control. Cell metabolism was evaluated by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2, 5diphenyl-tetrazolium bromide). The data were analyzed statistically by ANOVA, considering a significance of 5%. RESULTS: The values of cell viability ranged from 94.2% at 72h (SBU) to 109.6% at 48h (SB). The mean percentage of viability after exposure to the extracts of SB, SSAB and SBU were 103.2%, 100.63% and 97.43%, respectively. There was no statistically significant difference (p= 0.342) between the experimental and negative control groups. CONCLUSIONS: At all exposure times, all adhesives tested in this study presented no cytotoxicity to Vero cells in vitro. Key words:Biocompatibility, cytotoxicity, dental adhesives, Vero cells.
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The purpose of this study was to test the usefulness of some morphometric parameters of the teeth in 5 different age groups through images of Cone Beam CT. 118 upper central incisors clinically acquired of 60 women and 58 men aged between 22 and 70 years were selected. The hard tissue volume and the pulp cavity volume of each tooth was obtained and computed by the software DentalSlice(®) and the measures were assessed (pulp cavity volume, hard tissue volume, tooth volume and pulp cavity/tooth volume ratio). The pulp cavity volume and the pulp cavity/tooth volume ratio showed significant differences between age groups (p < 0.001). Linear regression analysis showed a coefficient of determination of 0.21 which suggests that there is a weak correlation between the pulp cavity/tooth volume ratio and age.
Assuntos
Tomografia Computadorizada de Feixe Cônico , Cavidade Pulpar/diagnóstico por imagem , Incisivo/diagnóstico por imagem , Adulto , Determinação da Idade pelos Dentes/métodos , Idoso , Brasil , Feminino , Odontologia Legal , Humanos , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Modelos Lineares , Masculino , Maxila , Pessoa de Meia-Idade , Adulto JovemRESUMO
A perda dentária possui forte impacto na qualidade de vida, ocasionando problemas de ordem funcional e emocional. Após a perda do elemento dental, o rebordo alveolar passa por um processo de reabsorção e isso impossibilita a colocação de implantes, por esse motivo, o enxerto ósseo é a técnica de escolha para solucionar tal problema. O objetivo deste estudo foi avaliar a morfologia da região retromolar, uma área utilizada para remoção de enxertos ósseos, em imagens de tomografia computadorizada (TC) tipo cone-beam para a reconstrução volumétrica, com a finalidade de determinar a altura e a espessura da área doadora disponível nessa região, utilizando como referência as corticais do canal mandibular e estabelecer uma média de quantidade óssea dessa região em uma amostra da cidade do Recife, Brasil. Material e Métodos: nesta amostra foram incluídos pacientes de ambos os sexos, com idade acima de 28 anos e sem limite de idade, que tinham presença dos segundos molares superiores e ausência de terceiros molares inferiores. A ferramenta de mensuração linear em milímetros foi utilizada para obter as medidas. Resultados e Conclusão: os homens apresentaram maior altura do canal mandibular do que as mulheres (p < 0,001), o sexo feminino apresentou maior quantidade de osso medular em comparação com o sexo oposto (p < 0,001), a quantidade de osso compacto foi relativamente maior do que o osso medular em ambos os gêneros
Tooth loss has a strong impact on quality of life, causing functional and emotional problems. After tooth loss, the alveolar ridge undergoes resorption and this precludes implant placement; therefore, bone grafting techniques are indicated for such situations. The aim of this study is to evaluate the morphology of the retromolar region through CBCT scanning for available heights and widths using the mandibular canal as reference point in a population of Recife, Brazil. Materials and methods: 200 patients of both genders above 28 years with their second molar teeth and the lack of lower third molars were selected. A linear measurement tool was used to generated data. Results and conclusions: from the final 60 selected patients and within the limits of this study, the height of the alveolar mandibular canal was higher in male than in female (p < 0.001), the female presented more medulary bone (p < 0.001), and the compact bone quantity was higher than medulary bone for both genders.
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Humanos , Masculino , Feminino , Adulto , Anatomia , Transplante ÓsseoRESUMO
Objetivo: avaliar o nível de conhecimento, de aplicação e de cobrança da Ergonomia na prática clínica. Material e Métodos: estudo descritivo, observacional de corte transversal realizado com 174 alunos, de ambos os gêneros, regularmente matriculados entre o 4º e o 9º período do currículo antigo de uma Faculdade de Odontologia do sistema público de educação superior em Pernambuco, por meio de um questionário contendo 16 conhecimentos básicos de Ergonomia e 10 situações ergonômicas a serem exigidas pelas disciplinas clínicas. Resultados: a amostra foi predominantemente feminina (67,2%). Como o conceito mais apreendido pelos estudantes, tem-se: No conceito de ergonomia não podem faltar às palavras conforto, segurança e eficiência (92,0%), e o menos apreendido foi o seguinte: A maioria dos equipos da faculdade é conceito ISO/FDI 3/ (13,2%). Apenas um conhecimento se associou ao gênero, dois, à idade, e treze, ao período cursado. A melhor taxa de acertos foi do 9º período, e a pior, a do 7º. A situação ergonômica mais exigida pelas disciplinas se relacionou ao uso correto do EPI (50,7%), e a menos exigida, à ginástica laboral (2,7%). A disciplina com maior média de exigência dos conhecimentos de Ergonomia e de sua aplicação foi a Clínica Integrada II (2,40), e a de menor média, a Prótese Fixa II (1,55). Todas as situações ergonômicas se associaram ao período cursado. Conclusões: os conhecimentos de Ergonomia perdem-se ao longo do tempo, e essa perda se deve, em parte, à falta de exigência das disciplinas. A disciplina de Clínica Integrada II é quem mais exige esses conhecimentos e sua aplicação.
Objective: To investigate the level of ergonomics' knowledge and its application in clinical practice. Material and Methods: descriptive and observational study of a sectional cross realized with 174 students, of both genders enrolled between 4th and 9th semesters of the old curriculum of the Dentistry's Faculty of the higher education public system in Pernambuco by applying a survey containing 16 regards basic knowledge of Ergonomics and 10 ergonomic situations to be required by the clinical disciplines. Results: The sample was predominantly female (67.2%). The concept was perceived by the most in the Concept of ergonomics can not miss words to comfort, safety and efficiency (92.0%) and was apprehended less Most equipos of faculty concept is ISO / FDI 3 / (13.2%). Only a knowledge is linked to gender, to age two, and thirteen of the period attended. The best accuracy rate was the 9th time and the worst of the 7. The most ergonomic situation was related disciplines required by the correct use of PPE (50.7%) and less required was related to gymnastics (2.7%). The discipline with the highest average requirement of ergonomics' knowledge and its application was the Integrated Clinic II (2.40) and lowest average was the Fixed Prosthodontics II (1.55). All cases were associated with ergonomic period attended. Conclusions: The knowledge of ergonomics are lost over time and this loss is partly due to lack of demand disciplines. Discipline of Integrated Clinic II is looking more demands that knowledge and its application.