RESUMO
Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 °C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels in WAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.
Assuntos
Tecido Adiposo Marrom , Temperatura Baixa , Hormônios Peptídicos , Termogênese , Animais , Humanos , Masculino , Camundongos , Tecido Adiposo Marrom/efeitos dos fármacos , Tecido Adiposo Marrom/fisiologia , Tecido Adiposo Branco/metabolismo , Camundongos Endogâmicos C57BL , Termogênese/efeitos dos fármacos , Termogênese/fisiologia , Proteína Desacopladora 1/metabolismo , Hormônios Peptídicos/farmacologia , Hormônios Peptídicos/fisiologiaRESUMO
Neoplastic disease is common in pet birds, particularly in psittacines, and treatment should be primarily aimed at tumor eradication. Nineteen cases of pet birds submitted to diagnostic and/or therapeutic surgical procedures due to neoplastic disease characterized by the presence of visible masses were retrospectively analyzed; affected species, types of neoplasms and respective locations, and outcomes of surgical procedures were determined. All birds undergoing surgery belonged to the order Psittaciformes; the Blue-fronted parrot (Amazona aestiva) was the prevalent species. Lipoma was the most frequent neoplasm in the sample studied. Most neoplasms affected the integumentary system, particularly the pericloacal area. Tumor resection was the most common surgical procedure performed, with high resolution and low recurrence rates.
RESUMO
In this work we report the vasorelaxant activity of 7-ß-O-glycosides obtained with biosynthesis of naringenin-7-ß-O-glycoside (3) and quercetin-7-ß-O-glycoside (4). These compounds were obtained from naringenin (1) and quercetin (2) glycosylation catalyzed by Beauveria bassiana ATCC 7159. Screening of the best strain as a catalyst for glycosylation was carried out and the reaction conditions established. Cultures were grown in PDSM medium for 7 days at 27 °C. After purification by reverse-phase preparative HPLC, naringenin-7-ß-O-glycoside (3) and quercetin-7-ß-O-glycoside (4) were identified by (1)H and (13)C NMR. The right position and ß-configuration of the glucose was determined through HSQC and HMBC experiments. The vasorelaxation potential of naringenin, quercetin and its glycosylated derivatives was evaluated using isolated aorta in vitro models. Interestingly, results suggest that vasorelaxation properties of naringenin, rutin and its glycosides are due to different pathways.
Assuntos
Flavanonas/biossíntese , Flavanonas/farmacologia , Flavonoides/química , Glucosídeos/biossíntese , Glucosídeos/farmacologia , Quercetina/análogos & derivados , Vasodilatação/efeitos dos fármacos , Vasodilatadores/farmacologia , Animais , Aorta/efeitos dos fármacos , Beauveria/metabolismo , Biocatálise , Flavanonas/química , Flavanonas/metabolismo , Flavonoides/metabolismo , Glucosídeos/química , Glicosilação , Técnicas In Vitro , Masculino , Estrutura Molecular , Quercetina/biossíntese , Quercetina/química , Quercetina/isolamento & purificação , Quercetina/metabolismo , Quercetina/farmacologia , Ratos Wistar , Vasodilatadores/isolamento & purificaçãoRESUMO
Avaliaram-se retrospectivamente as cirurgias realizadas em aves no Serviço de Cirurgia de Pequenos Animais do Hospital Veterinário da Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, durante período de oito anos. De um total de 90 intervenções cirúrgicas para diagnóstico e/ou tratamento de afecções, 27 foram ortopédicas e 63 de tecidos moles. Quanto ao percentual de cirurgias ortopédicas realizadas segundo as diferentes ordens, observou-se: Psittaciformes 85,19%, Piciformes 7,41%, Anseriformes 3,70% e Falconiformes 3,70%. Para as de tecidos moles os Psittaciformes representaram 92,06%, Columbiformes 3,17%, Passeriformes 3,17% e Anseriformes 1,60%. Entre os tipos de afecções ortopédicas encontradas as fraturas apresentaram a maior ocorrência (88,90%), seguidas de luxação (3,70%), avulsão traumática de extremidade (3,70%) e artrite/osteomielite (3,70%). Dentre as afecções cirúrgicas de tecidos moles as neoplasias apresentaram a maior ocorrência (30,15%), seguidas das neoformações cutâneas ou de anexos não neoplásicos (17,46%), neoformações cutâneas sem diagnóstico (7,94%), distocia (7,94%), fístula de papo (7,94%), hérnia abdominal (4,76%), sinusite (4,76%), gangrena de extremidade de membros (3,17%), perfuração de esôfago (3,17%), prolapso de cloaca (3,17%), "Necrose avascular de dígito" (1,59%), ferida na região da quilha (1,59%), perfuração de cavidade celomática (1,59%), neoformação em cavidade celomática sem diagnóstico (1,59%), corpo estranho em trato gastrointestinal (1,59%) e otite (1,59%). A distribuição das afecções cirúrgicas segundo as espécies acometidas mostrou o "grupo dos papagaios", representado em sua maioria por espécies do gênero Amazona, como prevalente. O conhecimento das afecções cirúrgicas e espécies de aves mais acometidas acrescentam informações para aqueles que já atuam nesta área e servem como indicador de estudo para futuros cirurgiões de aves.
The study assessed retrospectively avian surgeries at the Service of Small Animal Surgery, Veterinary Hospital of the Faculty of Veterinary Medicine and Animal Science, University of São Paulo, performed during eight years. A total of 90 procedures was done for diagnosis or treatment of surgical disorders. From these, 27 were orthopedic and 63 soft tissue surgeries. Considering the orthopedic surgeries performed according to orders we observed: Psittaciformes 85.19%, Piciformes 7.41%, Anseriformes 3.70%, and Falconiformes 3.70%; for soft tissues, the Psittaciformes represented 92.06%, Columbiformes 3.17%, Passeriformes 3.17%, and Anseriformes 1.60%. Fractures presented the highest incidence among orthopedic disorders (88.90%), followed by luxation (3.70%), traumatic avulsion of the end (3.70%) and arthritis/osteomyelitis (3.70%). Among the surgical disorders of soft tissue, neoplasms accounted in 30.15%, followed by cutaneous neoformation or non-neoplastic annexes (17.46%), undiagnosed cutaneous neoformations (7.94%), dystocia (7.94%), crop fistula (7.94%), abdominal hernia (4.76%), sinusitis (4.76%), gangrene of the extremities (3.17%), esophageal perforation (3.17%), cloaca prolapse (3.17%), "avascular necrosis of digit" (1.59%), wound in the region of the keel (1.59%), perforation of the coelomic cavity (1.59%), neoformation in the coelomic cavity without a diagnosis (1.59%), foreign body in the gastrointestinal tract (1.59%), and otitis (1.59%). The distribution of surgical disorders according to species affected showed that the "group of parrots", mainly represented by species of Amazonas genus, was prevalent. Knowledge of surgical and bird species most affected add information for those who work in this area and will be an indicator for future surgeons of birds.
Assuntos
Animais , Aves/cirurgia , Interpretação Estatística de Dados , Doenças dos Animais/diagnóstico , Doenças dos Animais/terapia , Lesões dos Tecidos Moles/veterinária , Osso e Ossos/lesõesRESUMO
Avaliaram-se retrospectivamente as cirurgias realizadas em aves no Serviço de Cirurgia de Pequenos Animais do Hospital Veterinário da Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, durante período de oito anos. De um total de 90 intervenções cirúrgicas para diagnóstico e/ou tratamento de afecções, 27 foram ortopédicas e 63 de tecidos moles. Quanto ao percentual de cirurgias ortopédicas realizadas segundo as diferentes ordens, observou-se: Psittaciformes 85,19%, Piciformes 7,41%, Anseriformes 3,70% e Falconiformes 3,70%. Para as de tecidos moles os Psittaciformes representaram 92,06%, Columbiformes 3,17%, Passeriformes 3,17% e Anseriformes 1,60%. Entre os tipos de afecções ortopédicas encontradas as fraturas apresentaram a maior ocorrência (88,90%), seguidas de luxação (3,70%), avulsão traumática de extremidade (3,70%) e artrite/osteomielite (3,70%). Dentre as afecções cirúrgicas de tecidos moles as neoplasias apresentaram a maior ocorrência (30,15%), seguidas das neoformações cutâneas ou de anexos não neoplásicos (17,46%), neoformações cutâneas sem diagnóstico (7,94%), distocia (7,94%), fístula de papo (7,94%), hérnia abdominal (4,76%), sinusite (4,76%), gangrena de extremidade de membros (3,17%), perfuração de esôfago (3,17%), prolapso de cloaca (3,17%), "Necrose avascular de dígito" (1,59%), ferida na região da quilha (1,59%), perfuração de cavidade celomática (1,59%), neoformação em cavidade celomática sem diagnóstico (1,59%), corpo estranho em trato gastrointestinal (1,59%) e otite (1,59%). A distribuição das afecções cirúrgicas segundo as espécies acometidas mostrou o "grupo dos papagaios", representado em sua maioria por espécies do gênero Amazona, como prevalente. O conhecimento das afecções cirúrgicas e espécies de aves mais acometidas acrescentam informações para aqueles que já atuam nesta área e servem como indicador de estudo para futuros cirurgiões de aves.(AU)
The study assessed retrospectively avian surgeries at the Service of Small Animal Surgery, Veterinary Hospital of the Faculty of Veterinary Medicine and Animal Science, University of São Paulo, performed during eight years. A total of 90 procedures was done for diagnosis or treatment of surgical disorders. From these, 27 were orthopedic and 63 soft tissue surgeries. Considering the orthopedic surgeries performed according to orders we observed: Psittaciformes 85.19%, Piciformes 7.41%, Anseriformes 3.70%, and Falconiformes 3.70%; for soft tissues, the Psittaciformes represented 92.06%, Columbiformes 3.17%, Passeriformes 3.17%, and Anseriformes 1.60%. Fractures presented the highest incidence among orthopedic disorders (88.90%), followed by luxation (3.70%), traumatic avulsion of the end (3.70%) and arthritis/osteomyelitis (3.70%). Among the surgical disorders of soft tissue, neoplasms accounted in 30.15%, followed by cutaneous neoformation or non-neoplastic annexes (17.46%), undiagnosed cutaneous neoformations (7.94%), dystocia (7.94%), crop fistula (7.94%), abdominal hernia (4.76%), sinusitis (4.76%), gangrene of the extremities (3.17%), esophageal perforation (3.17%), cloaca prolapse (3.17%), "avascular necrosis of digit" (1.59%), wound in the region of the keel (1.59%), perforation of the coelomic cavity (1.59%), neoformation in the coelomic cavity without a diagnosis (1.59%), foreign body in the gastrointestinal tract (1.59%), and otitis (1.59%). The distribution of surgical disorders according to species affected showed that the "group of parrots", mainly represented by species of Amazonas genus, was prevalent. Knowledge of surgical and bird species most affected add information for those who work in this area and will be an indicator for future surgeons of birds.(AU)
Assuntos
Animais , Aves/cirurgia , Doenças dos Animais/diagnóstico , Doenças dos Animais/terapia , Interpretação Estatística de Dados , Lesões dos Tecidos Moles/veterinária , Osso e Ossos/lesõesRESUMO
Nitric oxide (NO) has been demonstrated to be the primary agent in relaxing airways in humans and animals. We investigated the mechanisms involved in the relaxation induced by NO-donors, ruthenium complex [Ru(terpy)(bdq)NO(+)](3+) (TERPY) and sodium nitroprusside (SNP) in isolated trachea of rats contracted with carbachol in an isolated organs chamber. For instance, we verified the contribution of K(+) channels, the importance of sGC/cGMP pathway, the influence of the extra and intracellular Ca(2+) sources and the contribution of the epithelium on the relaxing response. Additionally, we have used confocal microscopy in order to analyze the action of the NO-donors on cytosolic Ca(2+) concentration. The results demonstrated that both compounds led to the relaxation of trachea in a dependent-concentration way. However, the maximum effect (E(max)) of TERPY is higher than the SNP. The relaxation induced by SNP (but not TERPY) was significantly reduced by pretreatment with ODQ (sGC inhibitor). Only TERPY-induced relaxation was reduced by tetraethylammonium (K(+) channels blocker) and by pre-contraction with 75mM KCl (membrane depolarization). The response to both NO-donors was not altered by the presence of thapsigargin (sarcoplasmic reticulum Ca(2+)-ATPase inhibitor). The epithelium removal has reduced the relaxation only to SNP, and it has no effect on TERPY. The both NO-donors reduced the contraction evoked by Ca(2+) influx, while TERPY have shown a higher inhibitory effect on contraction. Moreover, the TERPY was more effective than SNP in reducing the cytosolic Ca(2+) concentration measured by confocal microscopy. In conclusion, these results show that TERPY induces airway smooth muscle relaxation by cGMP-independent mechanisms, it involves the fluxes of Ca(2+) and K(+) across the membrane, it is more effective in reducing cytosolic Ca(2+) concentration and inducing relaxation in the rat trachea than the standard drug, SNP.