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The success of a prosthetic treatment is closely related to the periodontal health of the individual. The aim of this article was to review and present the importance of prosthetic restorative materials on the condition of the periodontium, the changes that occur in the composition of the subgingival microbiota and the levels of inflammatory markers in gingival crevicular fluid. Articles on the influence of different prosthetic restorative materials on subgingival microbiota and proinflammatory cytokines were searched for using the keywords "prosthetic biomaterials", "fixed prosthesis", "periodontal health", "subgingival microbiota", "periodontal biomarkers" and "gingival crevicular fluid" in PubMed/Medline, Science Direct, Scopus and Google Scholar. The type of material used for prosthesis fabrication together with poor marginal and internal fit can result in changes in the composition of the subgingival microbiota, as well as increased accumulation and retention of dentobacterial plaque, thus favoring the development of periodontal disease and prosthetic treatment failure. Biological markers have helped to understand the inflammatory response of different prosthetic materials on periodontal tissues with the main purpose of improving their clinical application in patients who need them. Metal-free ceramic prostheses induce a lower inflammatory response regardless of the fabrication method; however, the use of CAD/CAM systems is recommended for their fabrication. In addition, it is presumed that metal-ceramic prostheses cause changes in the composition of the subgingival microbiota producing a more dysbiotic biofilm with a higher prevalence of periodontopathogenic bacteria, which may further favor periodontal deterioration.
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Microbiota , Periodonto , Humanos , Ligamento Periodontal , Líquido do Sulco Gengival , Citocinas , BiomarcadoresRESUMO
Metabolic Syndrome (MetS) is a risk to develop metabolic-chronic degenerative disease, it is important to find natural alternatives to help decrease the risk. Mexican oregano has a traditional use in Mexican food, moreover, has pharmacologic effects that can help to reduce risk the metabolic syndrome. The aim of this work was to determine the effect of Mexican oregano ethanolic extract in metabolic syndrome in murine model. Ethanolic extract of Mexican oregano (Lippia graveolens) stem (Ext) had a favorable effect on biochemical markers in a murine model of MetS, induced by injection of monosodium glutamate (MSG). From newborn female mice, two groups were formed: control and the MSG groups, which received a dosage of 2 mg/kg of MSG via subcutaneous injection at the second and fourth postnatal day (PD 2,4), and 4 mg/kg at the PD 6, 8, 10 to induce obesity. On week 13, a part of the MSG group received Ext (group MSG + Ext) at 300 mg/kg, administered orally daily from week 13 to week 18. The results indicated that ethanolic extract of Lippia graveolens stem decreases the percentage of body fat, waist circumference, and body weight gain as well as cholesterol, serum triglyceride concentrations and systolic and diastolic pressure. Insulin and leptin hormone values showed a significant effect with the Ext administration. However, hepatic lipoperoxidation levels of MSG and MSG + Ext groups did not show any statistically significant differences between them, both being higher than the control group. Taking in consideration the results obtained in this study, it is concluded that the administration of Ext had a beneficial effect in the murine model with MetS. This is the first study demonstrating the potential of the polar fraction Lippia graveolens stem in MetS.
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C-phycocyanin (CPC) is an antihypertensive that is not still wholly pharmacologically described. The aim of this study was to evaluate whether CPC counteracts endothelial dysfunction as an antihypertensive mechanism in rats with 5/6 nephrectomy (NFx) as a chronic kidney disease (CKD) model. Twenty-four male Wistar rats were divided into four groups: sham control, sham-treated with CPC (100 mg/Kg/d), NFx, and NFx treated with CPC. Blood pressure was measured each week, and renal function evaluated at the end of the treatment. Afterward, animals were euthanized, and their thoracic aortas were analyzed for endothelium functional test, oxidative stress, and NO production. 5/6 Nephrectomy caused hypertension increasing lipid peroxidation and ROS production, overexpression of inducible nitric oxide synthase (iNOS), reduction in the first-line antioxidant enzymes activities, and reduced-glutathione (GSH) with a down-expression of eNOS. The vasomotor response reduced endothelium-dependent vasodilation in aorta segments exposed to acetylcholine and sodium nitroprusside. However, the treatment with CPC prevented hypertension by reducing oxidative stress, NO system disturbance, and endothelial dysfunction. The CPC treatment did not prevent CKD-caused disturbance in the antioxidant enzymes activities. Therefore, CPC exhibited an antihypertensive activity while avoiding endothelial dysfunction.
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Anti-Hipertensivos , Hipertensão , Ficocianina , Insuficiência Renal Crônica , Animais , Anti-Hipertensivos/farmacologia , Antioxidantes/metabolismo , Pressão Sanguínea , Suplementos Nutricionais , Endotélio Vascular , Hipertensão/tratamento farmacológico , Hipertensão/metabolismo , Masculino , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo III/metabolismo , Estresse Oxidativo , Ficocianina/farmacologia , Ratos , Ratos Wistar , Insuficiência Renal Crônica/tratamento farmacológico , Insuficiência Renal Crônica/metabolismo , VasodilataçãoRESUMO
ABSTRACT: Covid-19 is a viral disease that has spread throughout the world, becoming a pandemic. Dysgeusia and anosmia are some of its most frequent symptoms. The aim of the study was to determine the frequent signs and symptoms associated with COVID-19 patients. A cross-secional study from 370 patients with acute respiratory illness admitted by emergenc y services of a hospital in Acapulco. An institutional survey was applied to all patients as a data collection instrument, and a SARS-CoV-2 test, by RT-PCR processed by a certified laboratory. Statistical analysis was performed using the STATA V13 program. The numerical variables without normality were reported in medians, 25th and 75th percentiles, and the Mann W ithney U test was performed for differences between groups. The categorical variables were presented in percentages and differences between groups with Chi-square test. A generalized linear models (GLM) analysis was carried out to determine the most frequent symptoms and signs associated with COVID-19. Clinical signs and symptoms associated to COVID-19 in the bivariate análysis were dysgeusia, odynophagia, anosmia, arthralgia, myalgia, conjunctivitis, and age older than 40 years. In the final multivariate model only age older than 40 years (OR) 2.2; CI 95 % 1.3,3.8) and dysgeusia (OR 2.1; CI95 % 1.2,3.6) kept significance. Dysgeusia, odynophagia, anosmia, arthralgia, myalgia and conjunctivitis are clinical signs and symptoms that can appear in the early stages of the disease, so they could be important for an early diagnosis.
RESUMEN: El Covid-19 es una enfermedad viral que se ha extendido por todo el mundo, convirtiéndose en una pandemia. La disgeusia y la anosmia son algunos de sus síntomas más frecuentes. El objetivo del estudio fue determinar los signos y síntomas frecuentes asociados con los pacientes con COVID-19. Estudio transversal de 370 pacientes con enfermedad respiratoria aguda ingresados por los servicios de emergencia de un hospital de Acapulco. A todos los pacientes se les aplicó una encuesta institucional como instrumento de recolección de datos, y una prueba de SARS-CoV-2, por RT-PCR procesada por un laboratorio certificado. El análisis estadístico se realizó utilizando el programa STATA V13. Las variables numéricas sin normalidad se reportaron en medianas, percentiles 25 y 75, y se realizó la prueba U de Mann Withney para diferencias entre grupos. Las variables categóricas se presentaron en porcentajes y diferencias entre grupos con la prueba de Chi-cuadrado. Se realizó un análisis de modelos lineales generalizados (GLM) para determinar los síntomas y signos más frecuentes asociados a la COVID-19. Los signos y síntomas clínicos asociados a COVID-19 en el análisis bivariado fueron disgeusia, odinofagia, anosmia, artralgia, mialgia, conjuntivitis y edad mayor de 40 años. En el modelo multivariado final solo la edad mayor de 40 años (OR) 2,2; IC 95 % 1,3,3,8) y la disgeusia (OR 2,1; IC95 % 1,2,3,6) mantuvieron significanca estadística. Disgeusia, odinofagia, anosmia, artralgias, mialgias y conjuntivitis son signos y síntomas clínicos que pueden aparecer en etapas tempranas de la enfermedad, por lo que podrían ser importantes para un diagnóstico precoz.
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Quimioterapia neoadyuvante (NAC) en cáncer de mama permite conocer la sensibilidad del tumor al tratamiento, alcanzar respuesta patológica completa (pRC), está asociada a mejor supervivencia en cáncer de mama localmente avanzado. El objetivo de este estudio fue conocer el impacto de la pRC en la supervivencia en una cohorte de pacientes tratadas con NAC y cirugía. Se realizo un estudio de diseño observacional de tipo retrospectivo, correlacional, con un seguimiento promedio de 90 meses, de una cohorte de pacientes tratadas con NAC y cirugía desde enero del 2009 a diciembre del 2011. El análisis de datos se realizó mediante el software estadístico SPSS v22.0, para el análisis de supervivencia se utilizó el método de Kaplan Meier, para comparar supervivencias se consideró significativa una p<0,05. Entre las características principales de 199 pacientes, se destacan: edad joven a la presentación, elevado índice de proliferación y alta frecuencia del tipo inflamatorio. pRC ocurrió en el 14,1% de pacientes y la supervivencia global (SG) de acuerdo con la respuesta patológica se comparó entre aquellas pacientes que obtuvieron pRC, con las que tuvieron enfermedad residual, con una SG del 71,4% vs 45% respectivamente, con una diferencia significativa (p:0.009). En esta cohorte de pacientes la pRC impactó en la supervivencia en todos los subtipos clínico-patológicos, sobre todo en el subtipo triple negativo. Evaluar los datos en el entorno real es importante para definir estrategias y mejorar los resultados.
Neoadjuvant chemotherapy (NAC) in breast cancer allows knowing the sensitivity of the tumor to treatment, achieving pathological response complete (pRC), and is associated with better survival in locally advanced breast cancer. The objective of this study was to determine the impact of pRC on survival in a cohort of patients treated with NAC and surgery. A retrospective, correlational observational design study was carried out, with an average follow-up of 90 months, of a cohort of patients treated with NAC and surgery from January 2009 to December 2011. Data analysis was performed using the software SPSS v22.0 statistic, for the survival analysis the Kaplan Meier method was used, to compare survivals a p <0.05 was considered significant. Among the main characteristics of 199 patients, the following stand out: young age at presentation, high proliferation index and high frequency of the inflammatory type. pRC occurred in 14.1% of patients and overall survival (OS) according to the pathological response was compared between those patients who obtained pRC, with those who had residual disease, with an OS of 71.4% vs 45% respectively, with a significant difference (p: 0.009). In this cohort of patients, pRC impacted on survival in all clinicopathological subtypes, especially in the triple negative subtype. Evaluating data in the real environment is important to define strategies and improve results.
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Humanos , Feminino , Pessoa de Meia-Idade , Neoplasias da Mama/tratamento farmacológico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Quimioterapia Adjuvante/métodos , Terapia Neoadjuvante/métodos , Prognóstico , Taxa de Sobrevida , Estudos Retrospectivos , Resultado do Tratamento , Correlação de DadosRESUMO
BACKGROUND: Helicobacter pylori detection in asymptomatic children with suspected infection or with symptoms that suggest gastric pathology is problematic, since most of the methods depend on the endoscopic study, an invasive and expensive method. Non-invasive methods can be a feasible alternative but must be validated. The purpose of this study was to evaluate the concordance between H. pylori DNA detection in saliva and dental plaque by PCR, with antigen detection in stool by immunochromatography, among asymptomatic children in the state of Guerrero, Mexico. METHODS: Dental plaque, saliva, and stool samples were obtained from 171 children between 6 and 12 years old. H. pylori detection in saliva and dental plaque was performed by PCR using specific primers for the 16S rRNA gene, while the detection in stool samples was performed by immunochromatography using the CerTest kit. RESULTS: We found an overall H. pylori prevalence of 59.6% (102/171). Of the H. pylori positive children 18% (20/111) were positive in saliva samples, 28.1% (34/121) in dental plaque samples, and 50.4% (71/141) in stool samples. A higher prevalence was found in girls (64.7%, p = 0.002). Although some of the children declared some dyspeptic symptoms, these were no related to H. pylori. In conclusion, we found a high prevalence of H. pylori in asymptomatic children and the highest proportion was detected by stool antigen test, which was the most feasible method to detect H. pylori infection.
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ABSTRACT This study aims to estimate job satisfaction levels in graduated of dental school of Autonomous University of Guerrero, México, and determine which factors influence such satisfaction levels. A cross-sectional study was carried out in 144 dentists graduated from the dental school of Autonomous University of Guerrero through a validated survey. Logistic regression analysis was performed to obtain OR, p-value and confidence intervals (95% CI) in the STATA13 statistical program. The overall percentage of job satisfaction was 92%, while the variables that explained the model were, the role of the dentist (Owner or employed) (OR = 2.2 , p = 0.03 95% CI 1.1-4.4), and the level of satisfaction of the knowledge acquired within their academic dental school training (OR = 19.2, p = 0.00 95%CI 3.1-118). The professional satisfaction of a dentist is of great importance, because it impacts their performance at work, and is closely related to overall satisfaction of life, since both contribute reciprocally to the happiness of a person, as well as the general welfare in the community.
RESUMO Este estudo tem como objetivo estimar os níveis de satisfação no trabalho de graduados da Faculdade de Odontologia da Universidade Autônoma de Guerrero, México, e determinar quais fatores influenciam esses níveis de satisfação. Foi realizado um estudo transversal com 144 dentistas formados pela Faculdade de Odontologia da Universidade Autônoma de Guerrero por meio de um questionário validado. Uma análise de regressão logística foi realizada para obter OR, valor de p e intervalos de confiança (IC95%) no programa estatístico STATA13. O percentual geral de satisfação no trabalho foi de 92%, enquanto as variáveis que explicaram o modelo foram a função do dentista (proprietário ou funcionário) (OR = 2,2 , p = 0,03 IC 95% 1,1-4, 4) e o nível de satisfação com o conhecimento adquirido na formação acadêmica em odontologia (OR = 19,2 , p = 0,00 IC95% 3,1-118).. A satisfação profissional do dentista é de grande importância, pois impacta no seu desempenho no trabalho, e está intimamente relacionada à satisfação geral de vida, uma vez que ambas contribuem para a felicidade da pessoa, bem como para o bem-estar geral da comunidade.
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The objective of this study is to show the distribution of periodontal disease, risk factors, and importance of primary healthcare, for the improvement of clinical parameters. Two phases study transversal and nonrandomized trial (before - after), with educational intervention and conservative treatment, were carried out at Dental School of the Universidad Autónoma de Guerrero México, in 161 subjects who met the inclusion criteria. Oral healthcare education was carried out as well as conservative periodontal treatment, with six months follow up. Clinical measurements were performed with a Williams probe, O'Leary plaque index, calculus index and dental mobility Miller method. Periodontal disease was found on 82 % of all participants. Multinomial logistic regression analysis showed an odds ratio of 14.3 (95 % CI: 2.5, 82.1), 13.4 (95 % CI: 1.7, 103.5), 29.2 (95 % CI: 3.2, 260.9) and 68 (95 % CI: 6.6, 711.0) plaque in gingivitis, mild, moderate and severe chronic periodontitis, respectively. The longitudinal panel data analysis showed a significant effect (p <0.001) in the mean decrease of the clinical parameters after intervention, probing depth 0.4 mm (95 % CI: -0.5, -0.3), pockets depth 1.1 mm (95 % CI: -1.3, -0.9), amount of pockets 4.5 (95 % CI: -5.2, -3.7), bleeding 5.2 (95 % CI: -5.9, -4.5) and dental mobility 0.6 degrees (95 % CI: -0.7, -0.5). Primary healthcare is still the best option to improve the periodontal health in population who do not have access to specialty services. Dentists can achieve significant clinical improvement at very low cost, if they are aware of primary health care.
El objetivo del estudio fue mostrar la distribución de la enfermedad periodontal, los factores de riesgo y la importancia de la atención primaria de salud, para la mejora de los parámetros clínicos. Material y método: Estudio de dos fases transversal y no aleatorizado (antes - después), con intervención educativa y tratamiento conservador, realizado en la Facultad de Odontología de la Universidad Autónoma de Guerrero México, en 161 sujetos que cumplieron con los criterios de inclusión. Se llevó a cabo una educación sanitaria oral, así como un tratamiento periodontal conservador, con un seguimiento de seis meses. Las mediciones clínicas se realizaron con una sonda Williams, el índice de placa O'Leary, el índice de cálculo y el método Miller de movilidad dental. Resultados: se encontró enfermedad periodontal en el 82 % de todos los participantes. El análisis de regresión logística multinomial mostró un odds ratio de 14.3 (IC 95 %: 2.5, 82.1), 13.4 (IC 95 %: 1.7, 103.5), 29.2 (IC 95 %: 3.2, 260.9) y 68 (IC 95 %: 6.6, 711.0) placa en gingivitis, periodontitis crónica leve, moderada y grave, respectivamente. El análisis de datos del panel longitudinal mostró un efecto significativo (p <0.001) en la disminución media de los parámetros clínicos después de la intervención, profundidad de sondeo 0.4 mm (IC 95 %: -0.5, -0.3), profundidad de bolsillos 1.1 mm (IC 95 %: -1.3, -0.9), cantidad de bolsillos 4.5 (IC 95 %: -5.2, -3.7), hemorragia 5.2 (IC 95 %: -5.9, -4.5) y movilidad dental 0.6 grados (IC 95 %: -0.7, - 0.5). Conclusiones: la atención primaria de salud sigue siendo la mejor opción para mejorar la salud periodontal en la población que no tiene acceso a servicios especializados. Relevancia clínica: los dentistas pueden lograr una mejora clínica significativa a un costo muy bajo, si conocen la atención primaria de salud.
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Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Doenças Periodontais/epidemiologia , Atenção Primária à Saúde , Doenças Periodontais/terapia , Fatores Socioeconômicos , Modelos Logísticos , Saúde Bucal , Educação em Saúde , Estudos Transversais , Fatores de Risco , Seguimentos , Periodontite Crônica/epidemiologia , Tratamento Conservador , Gengivite/epidemiologia , MéxicoRESUMO
Periodontitis is modulated by a complex dysbiotic microbiota, these species stimulate upward the production of pro-inflammatory cytokines such as TNF-α, which, in turn, upregulates the production of bone resorption molecules. Enzymes such as MMP-8 and 9 have been associated with the destructive disease. This study evaluated the composition of periodontal microbiota with the checkerboard hybridization technique and its correlation with TNF-α, MMP-8, and MMP-9 evaluated with ELISA, of 80 patients (45 healthy, and 35 with chronic periodontitis). The frequency of the 18 species evaluated was higher in patients with bone loss compared with control group. TNF-α in gingival crevicular fluid was significantly higher in bone loss group (p < 0.01); MMP-8 (p = 0.34) by MMP-9 (p < 0.05) in bone loss group obtained lower values than in control group. Positive correlation of TNF-α was obtained with Aggregatibacter actinomycetemcomitans (rho = 0.38; p < 0.01), Fusobacterium nucleatum (rho = 0.25; p < 0.05) and Porphyromonas gingivalis (rho = 0.26; p < 0.05); negative correlation of MMP-8 with A. actinomycetemcomitans (rho = 0.26; p < 0.01), Capnocytophaga sputigena (rho = 0.33; p < 0.01), and F. nucleatum (rho = 0.21; p < 0.05); also negative correlation of MMP-9 with F. nucleatum (rho = 0.23; p < 0.05), P. gingivalis (rho = 0.23; p < 0.05), and Tannerella forsythia (rho = 0.26; p < 0.01). TNF-α increased due to the increase in each count of A. actinomycetemcomitans (ß = 0.57; p = 0.00). The presence of A. actinomycetemcomitans (ß = 1.88; p = 0.00), Campylobacter rectus (ß = 0.78; p = 0.01), F. nucleatum (ß = 0.65; p = 0.04), and P. gingivalis (ß = 0.65; p = 0.04) significantly increases TNF-α levels. TNF-α in gingival crevicular fluid, despite the minimal amounts collected, is a good biomarker of periodontal disease; since levels of TNF-α increases with the increase of the most harmful species to the periodontium.
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Líquido do Sulco Gengival , Microbiota , Humanos , Metaloproteinase 8 da Matriz , Metaloproteinase 9 da Matriz , Porphyromonas gingivalis , Prevotella intermedia , Fator de Necrose Tumoral alfaRESUMO
RESUMEN Introducción: La caries dental es el principal problema bucal que afecta a casi toda la población. La niñez y juventud son etapas cruciales para adquirir el hábito de higiene bucal adecuada y evitar daños a la cavidad oral en la edad adulta. Objetivo: Estimar la prevalencia de caries y los factores asociados en estudiantes de educación media superior de la Universidad Autónoma de Guerrero. Material y métodos: Estudio transversal en estudiantes de tres escuelas preparatorias de Chilpancingo, Gro. Un cuestionario autoadministrado preguntó sobre datos sociodemográficos, hábitos de higiene dental y la presencia de caries se definió a partir del índice CPOD (diente cariado, perdido y obturado). Se estimó razón de momios e intervalo de confianza de 95% como medida de asociación de los factores de riesgo. La estimación de las asociaciones en el análisis multivariado fue ajustada por el efecto del conglomerado. Resultados: La prevalencia de caries fue 91% (1,300/1,424), 18% (245/1,424) de los estudiantes tuvo obturados, 5% (67/1,424) tuvo una o más piezas dentales perdidas. El índice CPOD fue 5.31. Dos factores se asociaron con la presencia de caries: consumo de frituras, refrescos y golosinas (RMa 2.43, IC 95% 1.45-4.09), y la poca importancia dada a la salud bucal (RMa 1.48, IC 95% 1.01-2.18). Conclusiones: La prevalencia encontrada está en el rango de los reportes internacionales. Los dos factores asociados con la caries dental orientan qué acciones deben promoverse para el cuidado de la salud bucal en los estudiantes de educación media superior de la UAGro.
ABSTRACT Introduction: Tooth decay is the most important oral health problem affecting almost the whole population. Childhood and youth are crucial stages to acquire habits of proper oral hygiene to prevent damage to the oral cavity in adulthood. Objective: To measure the prevalence of dental caries and associated risk factors in students of middle education (preparatory school) at the Autonomous University of Guerrero (UAGro). Methods: Cross-sectional study including students from three preparatory schools (high schools) in Chilpancingo, Guerrero. A self-administered questionnaire collected sociodemographic and dental hygiene information; the presence of caries was defined according to the DMFT index (decayed, filled, and missing teeth). Odds ratio and 95% confidence intervals were estimated as measures of association for risk factors. Estimated associations in the multivariate analysis were adjusted by cluster effect. Results: The prevalence of caries was 91% (1,300/1,424); 18% (245/1,424) of students had filled teeth and 5% (67/1,424) had one or more missing teeth. A DMFT index score of 5.31 was found. Two factors were associated with the presence of caries: intake of snacks, sweets, and soft drinks (OR 2.43, 95% CI 1.45-4.09) and little attention to oral health (OR 1.48, 95% CI 1.01-2.18). Conclusions: The caries prevalence is in the range of international reports. The two factors associated with tooth decay should guide the actions to be implemented for promoting oral health among UAGro students of middle education.
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Background: The population of older adults is increasing worldwide, which brings attention to the importance of healthy aging. Adoption of healthy lifestyle activities such as participating in physical activity on a daily basis is key to maintaining physical and mental health. The aim of this study was to investigate the effects of participation in a 12-week multidimensional exercise program on health behavior and biopsychological factors of older adults living in Northeastern Mexico. Methods: A quasi-experimental study was conducted with 45 older adults (35 females and 10 males; M = 67.24 ± 5.73 years). The participants were assigned to an experimental group (EG; n = 23) that participated in a 12-week exercise program and a control group (CG; n = 22). Pre- and post-analyses of the exercise intervention data were carried out to investigate the participants' health-related variables including physical activity levels, blood pressure, self-esteem, depressive symptoms, and blood lipids profiles. Results: The results indicated that the exercise intervention contributed to significant improvements in the older adults' health-related variables for the EG when contrasted with the control group. For instance, the EG significantly improved systolic (p < 0.001) and diastolic (p < 0.027) blood pressure, blood lipids [e.g., cholesterol (p < 0.05)], triglycerides (p < 0.05), self-esteem (p < 0.005), and depressive symptoms (p < 0.002) as well as physical activity (p < 0.001) levels. The results also demonstrated that only those individuals in the EG diagnosed with disease benefited from improved self-esteem and physical activity levels when contrasted with their healthy counterparts.
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Introduction: vasoconstrictors are substances added to local anesthetics to lengthen their time of action, reduce their toxicity and enhance their local hemostatic effect. There is controversy about whether the use of vasoconstrictors in dental anesthetics has a negative effect on blood pressure and heart rate. Objective: determine the influence of vasoconstrictors added to dental anesthetics on blood pressure and heart rate. Methods: an experimental study was conducted with 120 patients divided in two groups of 60. These patients attended clinics in the Dental Academic Unit of the Autonomous University of Guerrero from July to December 2015. Group A was given just 3 percent mepivacaine, whereas Group B was given mepivacaine with epinephrine at a concentration of 1:100 000. Measurements were taken of the patients' heart rate, systolic blood pressure and diastolic blood pressure at two moments: at baseline before infiltration and 5 minutes after infiltration. Statistical analysis was based on Student's t-test for independent samples. Results: heart rate and systolic blood pressure did not yield any statistically significant value. Diastolic blood pressure was the only hemodynamic parameter showing a statistically significant difference (t= 2.3; p= 0.02). Conclusions: the results obtained coincide with those reported by similar studies. At the doses recommended, epinephrine is safe for healthy patients as well as for those with controlled cardiovascular conditions(AU)
Introducción: los vasoconstrictores son substancias que han sido añadidas a los anestésicos locales con la finalidad de aumentar su tiempo de acción, disminuir su toxicidad y aprovechar su efecto hemostático local. Existe controversia sobre si el uso de vasoconstrictores en los anestésicos dentales produce efectos negativos en la tensión arterial y la frecuencia cardiaca. Objetivo: determinar la influencia de los vasoconstrictores añadidos a la anestesia dental en la frecuencia cardiaca y la tensión arterial. Métodos: se realizó un estudio experimental en 120 pacientes divididos en dos grupos de 60, que acudieron a las clínicas de la Unidad Académica de Odontología de la Universidad Autónoma de Guerrero en el periodo julio-diciembre de 2015. Al grupo A se le administró mepivacaína simple al 3 por ciento, y al grupo B se le administró mepivacaína con epinefrina a una concentración de 1:100 000. Se realizaron tomas de frecuencia cardiaca, tensión arterial sistólica y tensión arterial diastólica en dos momentos. Se realizó una toma basal de frecuencia cardiaca, tensión arterial sistólica y diastólica antes de la infiltración, y una segunda toma 5 min posteriores a la infiltración. Se realizó la prueba estadística t de Student para grupos independientes. Resultados: la frecuencia cardiaca y la tensión arterial sistólica no obtuvieron significancia estadística. El único parámetro hemodinámico que tuvo una diferencia estadísticamente significativa fue la tensión arterial diastólica (t= 2,3; p= 0,02). Conclusiones : los resultados obtenidos en esta investigación, coinciden con los reportados en otros estudios similares. La epinefrina puede ser utilizada de manera segura a las dosis recomendadas tanto en pacientes sin enfermedad cardiovascular, como en pacientes con compromiso cardiovascular en control(AU)
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Humanos , Vasoconstritores/administração & dosagem , Pressão Arterial , Frequência Cardíaca/fisiologia , Anestesia Dentária/métodos , Anestésicos Locais/administração & dosagemRESUMO
Quercus sideroxyla is a wood species whose bark has phenolic compound and should be considered to be bioactive; the hypoglycemic and genotoxic properties of Q. sideroxyla bark were evaluated in this study. Total phenolic compound was determined in crude extract (CE) and organic extract (OE). The OE has the highest amount of phenols (724.1 ± 12.0 GAE/g). Besides, both CE and OE demonstrated effect over the inhibition of α-amylase in vitro. Hypoglycemic activity was assessed by glucose tolerance curve and the area under curve (UAC); OE showed the highest hypoglycemic activity. In addition, diabetes was induced by streptozotocin (65 mg/kg) and the extracts (50 mg/kg) were administered for 10 days; OE showed hypoglycemic effect compared with diabetic control and decreased hepatic lipid peroxidation. Acute toxicity and genotoxicity were evaluated in CE; results of acute toxicity did not show any mortality. Besides, the comet assay showed that CE at a dose of 100 mg/kg did not show any genotoxic effect when evaluated at 24 h, whereas it induced slight damage at 200 mg/kg, with the formation of type 1 comets.
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Brown algae and its carotenoids have been shown to have a positive influence on obesity and its comorbidities. This study evaluated the effect of Undaria pinnatifida and fucoxanthin on biochemical, physiological and inflammation markers related to obesity and on the expression of genes engaged on white adipose tissue lipid metabolism in a murine model of diet-induced obesity. The treatments improved energy expenditure, ß-oxidation and adipogenesis by upregulating PPARα, PGC1α, PPARγ and UCP-1. Adipogenesis was also confirmed by image analysis of the retroperitoneal adipose tissue, by measuring cell area, perimeter and cellular density. Additionally, the treatments, ameliorated adipose tissue accumulation, insulin resistance, blood pressure, cholesterol and triglycerides concentration in serum, and reduced lipogenesis and inflammation by downregulating acetyl-CoA carboxylase (ACC) gene expression, increasing serum concentration and expression of adiponectin as well as downregulating IL-6 expression. Both fucoxanthin and Undaria pinnatifida may be considered for treating obesity and other diseases related.
Assuntos
Dieta Vegetariana/métodos , Lipogênese/efeitos dos fármacos , Obesidade/dietoterapia , Phaeophyceae/química , Undaria/química , Xantofilas/farmacologia , Acetil-CoA Carboxilase/metabolismo , Adiponectina/sangue , Adiponectina/metabolismo , Tecido Adiposo Branco/metabolismo , Animais , Biomarcadores/metabolismo , Dieta Hiperlipídica/efeitos adversos , Modelos Animais de Doenças , Humanos , Inflamação/dietoterapia , Interleucina-6/metabolismo , Masculino , Síndrome Metabólica/dietoterapia , Obesidade/etiologia , PPAR alfa/metabolismo , PPAR gama/metabolismo , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/metabolismo , Ratos , Ratos Wistar , Proteína Desacopladora 1/metabolismo , Xantofilas/uso terapêuticoRESUMO
Introducción: la gingivitis es la enfermedad más común del periodonto, la medida profiláctica más efectiva y económica es la higiene bucal. Objetivos: estimar la prevalencia de gingivitis e identificar factores de riesgo asociados en universitarios de primer ingreso. Métodos: se realizó un estudio transversal de septiembre a diciembre de 2012. Se encuestó a 1 387 estudiantes de licenciatura de la Universidad Autónoma de Guerrero en Acapulco, México. No se estimó tamaño de la muestra porque se encuestó a todos los inscritos. Los instrumentos para la recolección de datos fue un cuestionario autoadministrado, formatos para inspección bucal y levantamiento de índice de higiene oral de Green y Vermillion simplificado, e índices de placa y gingivitis de Löe y Silness simplificados. El diagnóstico de gingivitis se hizo con los valores clínicos del índice de Löe y Silness considerando los puntajes de 0 a 0,09 como sano. La variable resultado fue gingivitis y las de causa: placa dentobacteriana, mala higiene bucal, tártaro, detritos, diastemas, aparatos de ortodoncia, apiñamiento dental y desgastes cervicales. Resultados: la prevalencia de gingivitis ponderada por conglomerado de escuelas fue 74,5 por ciento, de la cual 62,8 por ciento fue leve, 11,5 por ciento moderada, y 0,2 por ciento intensa. El 16 por ciento de los estudiantes, según el índice de Green y Vermillion, tuvo mala higiene y 87,3 pore ciento tuvo placa según criterio de Löe y Silness. Ajustando por conglomerados y los demás factores, cuatro factores fueron asociados a gingivitis: mala higiene bucal (OR 2,58 IC 95 por ciento 1,32-5,05), detritos (OR 2,62 IC 95 por ciento 1,49-4,58), diastemas (OR 1,72; IC 95 por ciento 1,13-2,64) y desgastes en cervical (OR 0,49; IC 95 por ciento 0,24-1,00). Conclusiones: la prevalencia de gingivitis se encontró dentro del rango reportado en otros estudios. La mala higiene bucal, detritos y diastemas fueron los factores de riesgo asociados a gingivitis(AU)
Introduction: gingivitis is the most common periodontal disease. The most effective prophylactic measure against this condition is oral hygiene. Objectives: estimate the prevalence of gingivitis and identify associated risk factors among first year university students. Methods: across-sectional study was conducted from September to December 2012 based on a survey applied to 1 387 students from the Autonomous University of Guerrero in Acapulco, Mexico. Sample size was not estimated because all the students registered were surveyed. The data collection tools used were a self-applied questionnaire, oral examination forms, the simplified Green and Vermillion oral hygiene index, and the simplified Löe and Silness plaque and gingivitis indices. Gingivitis was diagnosed with values on Löe and Silness indices, where scores from 0 to 0.09 were considered healthy. The result variable studied was gingivitis. Cause variables were dental plaque, poor oral hygiene, tartar, detritus, diastema, orthodontic appliances, dental crowding and cervical wear. Results: prevalence of gingivitis estimated by school cluster was 74.5 percent, of which 62.8 percent was mild, 11.5 percent was moderate and 0.2 percent was severe. According to the Green and Vermillion index, 16 percent of the students had poor hygiene, and according to the Löe and Silness indices 87.3 percent had plaque. Adjustment by clusters and other variables revealed four factors associated with gingivitis: poor oral hygiene (OR 2.58; CI 95 percent; 1.32-5.05), detritus (OR 2.62; CI 95 percent; 1.49-4.58), diastema (OR 1.72; CI 95 percent; 1.13-2.64) and cervical wear (OR 0.49; CI 95 percent; 0.24-1.00). Conclusions: prevalence of gingivitis was found to be within the range reported by other studies. Poor oral hygiene, detritus and diastema were the risk factors associated with gingivitis(AU)
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Coleta de Dados , Gengivite/epidemiologia , Higiene Bucal/efeitos adversos , Fatores de Risco , Estudos TransversaisRESUMO
Introducción: la medición de los niveles de satisfacción de los usuarios es parte fundamental para evaluar la calidad de los servicios de salud bucal. Objetivo: estimar la frecuencia de satisfacción y sus factores asociados en los usuarios de los servicios odontológicos en una jurisdicción sanitaria de Guerrero, México. Métodos: se realizó estudio transversal analítico en una muestra de 493 usuarios de los servicios de salud bucal mayores de 18 años de edad, a los que se aplicó cuestionario estructurado de 51 preguntas posterior a la atención dental. Se obtuvo información sobre datos sociodemográficos, satisfacción (variable resultado) y percepción del servicio odontológico recibido. Resultados: dos variables tuvieron efecto sobre la satisfacción: trato del estomatólogo y de la enfermera. La mayor fuerza de asociación (OR 7,37, cl IC 95 por ciento 3,73 35,78, RD 0,4 827 con cl IC 95 por ciento 0,3 332-0,6 322, ganancia 26/1 000) y ganancia se observó en la variable trato del estomatólogo. Tres variables tuvieron efecto sobre expectativa del usuario: trato del estomatólogo, comodidad de la sala de espera y del sillón de dental. La mayor fuerza de asociación (OR a 8,54, cl IC 95 por ciento 2,43 30,15) se observó en la variable trato del estomatólogo. La mayor ganancia la obtuvo comodidad del sillón dental (RD 0,2 924, cl IC 95 por ciento 0,1 894 0,3 855, ganancia 63/1 000). Se encontró alto nivel de satisfacción (88 por ciento, 434/493) con el servicio recibido, y un cumplimiento de la expectativa de los usuarios de 59 por ciento (285/484). Conclusión: el trato otorgado por el personal de salud es el principal aspecto donde las autoridades deberán implementar acciones para incrementar los niveles de satisfacción de los usuarios de los servicios dentales. El mejoramiento del trato a los usuarios por el equipo de salud bucal, estomatólogo y enfermera, deben considerarse dentro de los programas de mejora continua de estos servicios(AU)
Introduction: measurement of user satisfaction levels is an essential aspect of the quality evaluation of dental services. Objective: estimate the frequency of satisfaction and associated factors among users of dental services in a health area of Guerrero, Mexico. Methods: a analytical cross-sectional study was conducted of a sample of 493 users of dental services aged over 18 years who answered a structured questionnaire containing 51 questions after receiving dental care. Data were obtained about sociodemographics, satisfaction (variable result) and perception of the dental service received. Results: two variables had an effect on user satisfaction: attitude of the dentist and the nurse. The strongest association (OR 7.37, cl CI 95 percent; 3.73-35.78, RD 0.4827 with cl CI 95 percent; 0.3332-0.6322, gain 26/1 000) and gain were found in the variable attitude of the dentist. Three variables had an effect on user expectations: attitude of the dentist, comfort in the waiting room and comfort of the dental chair. The strongest association (OR 8.54, cl CI 95 percent; 2.43-30.15) was found in the variable attitude of the dentist. The greatest gain was obtained by comfort of the dental chair (RD 0.2924, cl CI 95 percent; 0.1894-0.3855, gain 63/1 000). Satisfaction with the service received was found to be high (88 percent, 434/493). Fulfillment of user expectation was 59 percent (285/484). Conclusion: authorities should implement actions aimed at improving the attitude of health personnel toward patients, with a view to raising the level of user satisfaction with dental services. Improvement in the attitude of dental care personnel, dentist and nurse, toward patients should be included in programs for the continued development of these services(AU)
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Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Qualidade da Assistência à Saúde/estatística & dados numéricos , Inquéritos de Saúde Bucal/estatística & dados numéricos , Satisfação do Paciente/estatística & dados numéricos , Assistência ao Paciente/ética , Melhoria de Qualidade/estatística & dados numéricosRESUMO
Evaluation of the phenolic compounds and antioxidant activity of Cedrela odorata L. and Juglans regia L. bark extracts was performed in vitro. Juglans regia showed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect of J. regia and C. odorata in normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.
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BACKGROUND: The SLC38A4 gene is related to system 'A' activity, which seems to be related to impaired gluconeogenesis. The objective of this study was to determine whether the 292 C>T and 1304 G>A polymorphisms of SLC38A4 gene are associated with hyperglycaemia in humans. METHODS: A total of 227 individuals were enrolled in a case-control study, in which hyperglycaemia was defined by plasma glucose levels ≥95 mg/dL. Genotyping was carried out by using real-time polymerase chain reaction. RESULTS: The frequency of mutant alleles of SLC38A4 gene for single-nucleotide polymorphism (SNP) 1304 G>A was 23.6% and 30.2% for SNP 292 C>T. The frequency of allele T for the SNP 292 C>T in the case and control groups did not show significant differences, whereas the frequency of allele A for the SNP 1304 G>A was significantly higher in the case group than in the control group (p = 0.04). In the logistic regression analysis, the SNP 1304 G>A [odds ratio (OR) 1.78; 95%CI 1.04-3.05, p = 0.03] but not SNP 292 C>T (OR 1.41; 95%CI 0.80-2.47, p = 0.23) showed a significant association with hyperglycaemia. After adjusting by body mass index, waist circumference and triglycerides, the SNP 1304 G>A remained significantly associated with hyperglycaemia (OR 2.13; 95%CI 1.18-3.83, p = 0.03). Pair wise linkage disequilibrium showed correlation (D' > 0.82) between 292 C>T and 1304 G>A SNPs. Haplotype association with hyperglycaemia also showed significant association between both homozygous mutant alleles (A/T) and hyperglycaemia (OR 1.68; 95%CI 1.01-2.79, p = 0.048). CONCLUSIONS: Our results suggest that mutant allele A for SNP 1304 G>A of SLC38A4 gene is associated with hyperglycaemia.
Assuntos
Sistema A de Transporte de Aminoácidos/genética , Predisposição Genética para Doença , Hiperglicemia/genética , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Alelos , Índice de Massa Corporal , Estudos de Casos e Controles , Feminino , Frequência do Gene , Estudos de Associação Genética , Genótipo , Haplótipos , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Valproic acid (VPA) is a potent inducer of neural tube defects in human and mouse, its teratogenicity is associated with its potential to generation of free radicals and increase oxidative stress. Furthermore, spirulina (SP) has shown pharmacological properties against teratogenicity, which are attributed to its antioxidant potential. Accordingly, the present study was performed to investigate the influence of SP on the teratogenicity of VPA in imprinting control region mice and the possible mechanisms of action. VPA (sodium valproate) was administered intraperitoneally to mice on gestation day (GD) 8 at a dose of 600 mg/kg. SP was given orally at 125, 250, and 500 mg/kg daily from GD0 through GD18. The most common finding in fetuses with VPA exposure was exencephaly. SP decreased the incidence of this and other malformations and increased levels of superoxide dismutase, catalase, and glutathione peroxidase. In conclusion, these results illustrate the protective action of SP through its antioxidant activity against VPA-induced teratogenicity.