RESUMO
Twenty-eight fatty acids (C8:0 to C24:l n-9) were measured by gas chromatography in four normal cell lines (C3H / 10T1 / 2, CCD-18Co, CCD-25SK and CCD-37Lu) and seven cancer cell lines (C-41, Caov-3, LS-180, PC-3, SK-MEL-28, SK-MES-1 and U-87 MG). Results show differences in the content and proportions of fatty acids when comparing cancer cell lines with their normal counterparts. Cancer cell lines showed lower C20: 4 n-6, C24:1 n-9, polyunsaturated fatty acids (PUFA's) and ratios of C20:4 n-6 to C20:5 n-3 and C16:0 to C18:1 n-9 and stearic to oleic (SA/OA) than their normal counterparts. All cancer cell lines had SA/OA ratios lower than 7.0 while normal cell lines had ratios greater than 0.7 (p<0.05). In addition, the ratios of total saturated fatty acids (SFA) to PUFA'S and the concentration of C18:1 n-9, C18:2 n-6, C20:5 n-3 were higher in cancer cell lines as compared to normal cell lines. A positive correlation was detected between C16:0 and longer SFA'S (r = +0.511, p<0.05) in normal cell lines whereas a negative correlation (r=0.608, p<0.05) was obtained for malignant cell lines. Moreover, cancerous cell lines exhibited a particular desaturation defect and an abnormal incorporation of C18:2 n-6 and C20-4 n-6 fatty acids
Assuntos
Humanos , Camundongos , Ácidos Graxos/análise , Linhagem Celular Tumoral/química , Membrana Celular/química , Ácidos Graxos/metabolismo , Linhagem Celular Tumoral/metabolismo , Membrana Celular/metabolismoRESUMO
The term primitive neuroectodermal tumor (PNET) names a group of malignant neoplasms of presumed neural crest origin; they are composed of round small cells, and may be centrally located or outside. The former are currently seen in childhood, mostly in reference to the posterior fossa, and include medulloblastoma and neuroblastoma. Outside of the central nervous system includes peripheral neuroectodermal tumor of the bone (PNET-B, Tefft's tumor), Askin's tumor, and even Ewing's sarcoma. We describe a case in a young adult, with extremely unusual spinal localization, in which histopathological and immunohistochemical analysis were done; also, a review of some features is presented clinical-histopathological issues concerning related.
Assuntos
Tumores Neuroectodérmicos Primitivos , Neoplasias da Coluna Vertebral , Adulto , Biópsia , Feminino , Humanos , Imageamento por Ressonância Magnética , Tumores Neuroectodérmicos Primitivos/diagnóstico , Tumores Neuroectodérmicos Primitivos/patologia , Medula Espinal/patologia , Neoplasias da Coluna Vertebral/diagnóstico , Neoplasias da Coluna Vertebral/patologiaRESUMO
Se presentan los resultados de 619 pacientes con Insuficiencia Renal Crónica Terminal (IRCT) evaluados mediante dos cortes de tipo transversal realizados en enero de 1990 y enero de 1991 en todos los Sistemas de atención hospitalaria de Salud de Lima (Ministerio de Salud, Instituto Peruano de Seguridad Social-IPSS, Centro Privados de Hemodiálisis, Hospitales Militares y Clínicas Privadas). Se seleccionaron 619 de 782 pacientes evaluados en enero 1990 que completaron la siguiente información: Centro de atención en enero 1990 (CA), cobertura económica de atención en enero 1990 (CE), edad, causa de la IRCT (DX), ciudad de procedencia y estado final en enero 1991 (FIN). Los datos se evaluaron con análisis univariado, regresión múltiple, y ANOVA, usando el paquete estadístico EPI INFO V5. El 82 por ciento de los atendidos en Centros Privados de hemodiálisis, el 55.5 por ciento de le IPSS, el 48 por ciento en los Hospitales Militares y/o Clínicas Privadas, y 4 por ciento en los Hospitales del Ministerio de Salud estuvieron vivos en enero de 1991 (p<0.0001). El 65 por ciento de los que contaban con Seguro Social y el 33 por ciento de pacientes con sustento económico propio estuvieron vivos en enero del 91; mientras que ninguno de los que contaban con Seguro Particular o Institucional estuvieron vivos a la misma fecha. El 83 por ciento del grupo de edad de <20 años, el 64 por ciento entre 21-40 años, el 62 por ciento entre 41-60 y el 52 por ciento >60 años, estuvieron vivos en enero del 91. El 72 por ciento de los casos en Enfermedad Glomerular Primaria, el 63 por ciento de Nefropatía Intersticial sin enfermedad sistémica, el 58 por ciento con diagnóstico desconocido y el 46 por ciento con Nefropatía asociada a enfermedad sistémica, estuvieron vivos en enero del 91. Los resultados mostraron que el centro de atención, la cobertura económica de atención, la edad y la causa de insuficiencia renal crónica influyeron significativamente sobre el estado final (p<0.01).
Assuntos
Estudos de Casos e Controles , Insuficiência Renal CrônicaRESUMO
The association of coarctation of the aorta and pregnancy has been cause of concern among the various medical specialties involved in the care of patients with these conditions, as this cardiovascular abnormality is included in the list of disease potentially critical during pregnancy. The individually reported experience with this association has not been large, on account of the male predominance of this relatively rare congenital entity. The experience in the Internal Medicine Service of the "Concepción Palacios" Maternity Hospital of Caracas, Venezuela, between 1968 and 1985 is presented. Nine cases represented 2.9% of the congenital cardiovascular diseases, 0.84% of all cardiovascular diagnoses and 1.06 per 100.000 of all patients obstetrically attended during that period. In our experience, pregnancy is feasible in patients with coarctation, tolerance to gestation is generally good and fertility is not adversely affected, as well as prematurity and perinatal morbidity and mortality. The unique clinical situation of each patient, is to be nevertheless individually analyzed, especially in search for any other associated circulatory pathologic condition (s) which might render the patient more vulnerable than the average in the general group. The obstetric must prevail over the cardiologic criteria in the majority of cases, as to the mode of termination of pregnancy.
Assuntos
Coartação Aórtica/complicações , Complicações Cardiovasculares na Gravidez/epidemiologia , Adulto , Coartação Aórtica/patologia , Feminino , Humanos , Pessoa de Meia-Idade , Gravidez , Complicações Cardiovasculares na Gravidez/patologia , VenezuelaRESUMO
A case of megacalycosis is described in a newborn with multiple anomalies and enlarged kidneys. Uroradiological studies, TAC, ultrasound and nuclear medicine studies were practiced. The excretory urogram was by far the best technique to demonstrate this entity characterized by enlarged kidneys and uniform dilatation of all the calyces with the remainder of the collecting system normal. Voiding cystourethrography was normal as were renal function tests. This rare urological entity is a form of renal dysplasia which must not be confused with obstructive o refluxing hydronephrosis so that needless surgery is avoided.
Assuntos
Cálices Renais/anormalidades , Pelve Renal/anormalidades , Anormalidades Múltiplas , Diagnóstico Diferencial , Feminino , Humanos , Hidronefrose/diagnóstico , Recém-Nascido , Rim/anormalidades , Rim/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Ultrassonografia , UrografiaRESUMO
Se describe un caso de megacaliosis en un recien nacido con multiples anomalias y rinomes aumentados de tamano. Se le practicaron estudios urorradiologicos, TAC, ultrosonido y medicina nuclear. La urografia excretora fue la mejor tecnica para demostrar esta entidad caracterizada por grandes rinomes y dilatacion uniforme de todos los calices; el resto del sistema colector fur normal. La cistouretrografia fue normal, asi como las pruebas de funcion renal. Esta rara entidad urologica es una forma de displasia renal que pueda ser confundida con obstruccion o hidronefrosis por reflujo en la cual la cirugia esta contrindicada