RESUMO
This research evaluated H2TiO7 nanotubes (TiNTs) functionalized with 1 (1TiCN), 5 (2TiCN), and 10 (3TiCN) wt.% of chitosan for the removal of clonazepam by an adsorption/photocatalysis-conjugated method. The samples were immobilized on glass, and their mechanical stability was tested by washings. The functionalization of the samples was verified by the FTIR and DRS techniques. SEM images displayed nanotubes in the samples and thickness of 4.24 µm for the 2TiCN coating. The chemical composition of the 2TiCN coating was obtained by EDS. The XRD patterns evidenced chitosan and titanate phases in the functionalized samples. Furthermore, the 2TiCN coating was evaluated in the removal of clonazepam, reaching 80.79% (4.38 and 49.64% more than the TiNT and commercial TiO2 powders, respectively) after 240 min and being 6.88% more efficient after 4 reuses than the 2TiCN powders. OH- ions were the main oxidizing species found by scavenger tests. The surface area of 2TiCN (168.6 m2/g) was 2 times higher than that of TiNTs, and its bandgap (2.95 eV) was the lowest. Therefore, the 2TiCN coating is an excellent alternative to remove clonazepam.
RESUMO
Hematopoietic stem cells (HSCs) are capable of entering the cell cycle to replenish the blood system in response to inflammatory cues; however, excessive proliferation in response to chronic inflammation can lead to either HSC attrition or expansion. The mechanism(s) that limit HSC proliferation and expansion triggered by inflammatory signals are poorly defined. Here, we show that long-term HSCs (HSCLT) rapidly repress protein synthesis and cell cycle genes following treatment with the proinflammatory cytokine interleukin (IL)-1. This gene program is associated with activation of the transcription factor PU.1 and direct PU.1 binding at repressed target genes. Notably, PU.1 is required to repress cell cycle and protein synthesis genes, and IL-1 exposure triggers aberrant protein synthesis and cell cycle activity in PU.1-deficient HSCs. These features are associated with expansion of phenotypic PU.1-deficient HSCs. Thus, we identify a PU.1-dependent mechanism triggered by innate immune stimulation that limits HSC proliferation and pool size. These findings provide insight into how HSCs maintain homeostasis during inflammatory stress.
Assuntos
Células-Tronco Hematopoéticas/metabolismo , Inflamação/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Estresse Fisiológico/fisiologia , Transativadores/metabolismo , Animais , Ciclo Celular/fisiologia , Diferenciação Celular/fisiologia , Proliferação de Células/fisiologia , Homeostase/fisiologia , Imunidade Inata/fisiologia , Camundongos , Camundongos Endogâmicos C57BLRESUMO
PURPOSE: To assess the predictability, efficacy, safety, and complications of the PRL (CIBA Vision) phakic intraocular lens (pIOL) in the correction of high myopia. SETTING: Clínica Oftalmológica Pasteur, Santiago, Chile. METHODS: This prospective study comprised 53 eyes of 39 patients who received a PRL pIOL. The spherical equivalent (SE), uncorrected visual acuity (UCVA), efficacy index (postoperative UCVA/preoperative best spectacle-corrected visual acuity [BSCVA]), safety index (BSCVA/preoperative BSCVA), intraocular pressure (IOP), iridocorneal angle, and distance between the crystalline lens and PRL were prospectively assessed during the follow-up period. RESULTS: The mean patient age was 39 years. The mean power of the implanted pIOL was -12.73 diopters (D) +/- 2.87 (SD) (range -20.00 to -7.00 D), for a preoperative SE of -17.27 +/- 4.58 D (range -31.50 to -8.75 D). The mean preoperative BSCVA was 0.50 +/- 0.70 (logMAR equivalent), and the mean follow-up was 8 +/- 9.4 months. The mean postoperative SE was -0.23 +/- 1.05 D; 71.2% of eyes were within +/-1.00 D. Sixty percent of patients had UCVA of 20/40 or better, and 88.2% of patients had BSCVA of 20/40 or better. Ninety-two percent maintained or gained 1 or more lines of BSCVA; 5.7% lost more than 1 line. There was no significant change in IOP (P = .40), and the mean distance between the crystalline lens and pIOL was 370 microm (ultrasound) and 604 microm (optical coherence tomography). Late complications included 1 case each of retinal detachment and lens subluxation. CONCLUSIONS: Although PRL implantation in high myopia was predictable and effective, retinal detachment and IOL subluxations occurred. Other posterior chamber pIOLs should be used until the complications associated with the PRL pIOL are resolved.
Assuntos
Implante de Lente Intraocular/métodos , Cristalino/fisiologia , Lentes Intraoculares , Miopia/cirurgia , Acomodação Ocular/fisiologia , Adulto , Feminino , Humanos , Cristalino/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Estudos Prospectivos , Tomografia de Coerência Óptica , Ultrassonografia , Acuidade VisualRESUMO
La Siderosis es una complicación tardía y muy temida de la presencia de un cuerpo extraño férrico intraocular. Su diagnóstico ensombrece el pronóstico visual, debido a los cambios irreversibles sufridos por las estructuras comprometidas. Hoy en día es cada vez menos frecuente por el diagnóstico y manejo más oportuno. Reportamos una serie de tres casos atendidos en nuestro servicio. El tiempo en que se desarrollaron los cambios varió de ocho meses a tres años. Todas las estructuras oculares fueron comprometidas. El daño retinal a nivel de los fotorreceptores fue severo con aplanamiento de las ondas de electrorretinograma. La ecografía B fue el pilar fundamental para el diagnóstico en todos los casos. En un caso se tomó una muestra de tejido iridiano durante la vitrectomía y se envió a estudio de microscopia óptica y electrónica, evidenciándose el daño por el depósito de hierro en las estructuras celulares.