RESUMO
The present study reports the effect of shortening the prefreezing equilibration time with glycerol on the quality of frozen-thawed ejaculated sperm from four Mediterranean mountain ungulates: Cantabrian chamois (Rupicapra pyrenaica), Iberian ibex (Capra pyrenaica), mouflon (Ovis musimon) and aoudad (Ammotragus lervia). Ejaculated sperm from these species were divided into two aliquots. One was diluted with either a Tris-citric acid-glucose based medium (TCG-glycerol; for chamois and ibex sperm) or a Tris-TES-glucose-based medium (TTG-glycerol; for mouflon and aoudad sperm), and maintained at 5°C for 3h prior to freezing. The other aliquot was diluted with either TCG (chamois and ibex sperm) or TTG (mouflon and aoudad sperm) and maintained at 5°C for 1h before adding glycerol (final concentration 5%). After a 15min equilibration period in the presence of glycerol, the samples were frozen. For the ibex, there was enhanced (P<0.05) sperm viability and acrosome integrity after the 3h as compared with the 15min equilibration time. For the chamois, subjective sperm motility and cell membrane functional integrity were less (P<0.05) following 15min of equilibration. In the mouflon, progressive sperm motility and acrosome integrity was less (P<0.05) when the equilibration time was reduced to 15min. For the aoudad, the majority of sperm variables measured were more desirable after the 3h equilibration time. The freezing-thawing processes reduced the sperm head size in all the species studied; however, the equilibration time further affected the frozen-thawed sperm head variables in a species-dependent fashion. While the equilibration time for chamois sperm might be shortened, this appears not to be the case for all ungulates.
Assuntos
Criopreservação/veterinária , Crioprotetores/farmacologia , Glicerol/farmacologia , Preservação do Sêmen/veterinária , Ovinos/fisiologia , Animais , Criopreservação/métodos , Crioprotetores/administração & dosagem , Glicerol/administração & dosagem , Masculino , Preservação do Sêmen/métodos , Ovinos/classificação , Especificidade da Espécie , Motilidade dos Espermatozoides , TemperaturaRESUMO
OBJECTIVE: To present current clinical experience and the clinical outcomes in the management of patients with adult Still disease. MATERIALS AND METHODS: A retrospective study of a case series was conducted on of 17 cases diagnosed with adult Still disease during a period between 2009 and 2014 in 2 tertiary hospitals in Santiago de Cali. RESULTS: Of the 17 cases diagnosed and treated, it was found that 50% of patients had an elevated ferritin five times its normal value, being useful in the diagnosis. Less than 50% of cases were under 40 years, and about 50% of the population required combined treatment, with two patients requiringbiological therapy to control their symptoms. Yamaguchi criteria were used for diagnostic correlation. CONCLUSIONS: Adult Still's disease is a rare disease that requires a high index of suspicion, but it must be a disease to rule out, and it always requires management generally combined with schemes to improve the quality of life of patients.
OBJETIVO: Dar a conocer la experiencia clínica en el mundo real y los resultados clínicos del manejo de los pacientes con enfermedad de Still del adulto. MATERIALES Y MÉTODOS: Se realiza un estudio retrospectivo de serie de casos, de 17 casos con diagnóstico de enfermedad de Still del adulto, en 2 clínicas de nivel 3 de Santiago de Cali, Colombia, en el periodo comprendido entre 2009 y 2014. RESULTADOS: De los 17 casos diagnosticados y tratados se encontró que en el 50% de los pacientes había una elevación de ferritina de 5 veces su valor normal, siendo útil en el diagnóstico, menos del 50% de los casos fueron en menores de 40 anos, alrededor del 50% de la población requirió tratamiento combinado y 2 pacientes requirieron terapia biológica para el control de sus síntomas. Se usaron los criterios de Yamaguchi para la correlación diagnóstica. CONCLUSIONES: de descarte y, generalmente, siempre requiere manejo con esquemas combinados para mejorar la calidad de vida del paciente.
Assuntos
Humanos , Doença de Still de Início Tardio , Febre de Causa DesconhecidaRESUMO
A method for cryopreserving wild ibex sperm at high cooling rates was developed. To design a freezing solution based on Tris, citric acid, and glucose (TCG), two preliminary experiments were performed using glycerol (GLY) and dimethyl sulfoxide (DMSO) at different concentrations (5%, 10%, 20%). The 10% GLY + 10% DMSO combination reduced (P < 0.05) frozen-thawed sperm motility, which reached a minimum when 20% GLY + 20% DMSO was used. In the second experiment, sperm tolerance to three sucrose concentrations was evaluated (100-mM sucrose, 300-mM sucrose, 500-mM sucrose). Frozen-thawed sperm motility and sperm viability decreased (P < 0.05) at concentrations above 300 mM. The ultrarapid cooling procedure finally used involved a TCG egg yolk (ey)-based extender with 100-mM sucrose, either alone or with 5% GLY with or without BSA. Two warming procedures (37 °C vs. 60 °C) were also evaluated. The TCG ey with 100-mM sucrose but without GLY/BSA returned the best sperm quality variables. Slow warming at 37 °C strongly affected (P < 0.05) sperm motility and viability in all groups. Sperm selection by density gradient centrifugation produced no motile sperm when slow warming was performed. In contrast, when fast warming was used, sperm selection increased (P < 0.05) percentage of motility, viability, and the percentage of sperms with intact acrosomes. Heterologous in vivo fertilization involving domestic goats was performed to evaluate the in vivo fertilization capacity of the ultrarapidly cooled cryopreserved sperm (in TCG-ey + 100 mM sucrose), with warming undertaken at 60 °C. Inseminations of domestic goats resulted in three pregnancies (3 of 16, 18.7% fertility). In conclusion, ibex spermatozoa are strongly sensitive to high concentrations of permeable cryoprotectants and sucrose. However, the combination of ultrarapid cooling, using TCG-ey + 100-mM sucrose, and fast warming at 60 °C, followed by sperm selection by density gradient centrifugation to collect the motile sperm, has a positive effect on sperm viability.
Assuntos
Criopreservação/veterinária , Cabras/fisiologia , Espermatozoides/fisiologia , Animais , Criopreservação/métodos , Feminino , Fertilidade , Inseminação Artificial/veterinária , Masculino , Gravidez , Fatores de TempoRESUMO
Two experiments were conducted to study the effect of shortening the equilibration time with the cryoprotectant glycerol before freezing epididymal sperm recovered postmortem from Iberian ibex. In the first experiment, the standard equilibration time of 3 hours was compared with 2 hours, and subjective sperm motility and quality of movement were greater (P < 0.05) in the latter group. In the second experiment, reducing the equilibration time from 2 hours to 15 minutes did not affect sperm motility (evaluated subjectively and objectively), viability, acrosomal integrity, or membrane functional integrity. In conclusion, shortening the equilibration time can be used as a technique to simplify the cryopreservation process and this provides practical advantages under field conditions.
Assuntos
Criopreservação/veterinária , Epididimo/citologia , Glicerol , Cabras/fisiologia , Preservação do Sêmen/veterinária , Espermatozoides/fisiologia , Animais , Criopreservação/métodos , Masculino , Preservação do Sêmen/métodos , Motilidade dos Espermatozoides , Fatores de TempoRESUMO
Antecedentes. La Unidad de Cuidados Intensivos Pediátricos del Hospital Infantil de la Cruz Roja Rafael Henao Toro de la ciudad de Manizales, Colombia, se ha convertido en referencia para esta zona del país. No se cuenta con estudios en los que se analice la epidemiología de esta unidad, datos que podrían ser importantes en varios sentidos. Objetivo. Realizar un estudio epidemiológico de la población que ingresa a la Unidad de Cuidados Intensivos Pediátricos en el Hospital Infantil Universitario de Manizales (Colombia), años 2006, 2007. Material y métodos. Estudio de corte transversal. Las variables empleadas fueron: edad, fecha de ingreso y egreso, género, procedencia, seguridad social, causa de ingreso, tiempo de estancia, procedimientos utilizados, causa de egreso, destino de alta y causas de mortalidad. Resultados. Se presenta una serie de 341 pacientes pediátricos críticos cuya edad media fue 6.8 años, género masculino 61%, de aéreas rurales 56.2%. Las enfermedades más prevalentes son las infecciosas y entre ellas la de origen pulmonar; mortalidad global de 14.15%, con no respuesta a la resucitación de 89%. Los procedimientos más empleados fueron los siguientes: 26.9% ventilación mecánica, 25.2% sonda gástrica, 22.4% sonda vesical, y 21.7% catéter venoso central. Conclusiones. Con la investigación realizada se logró la identificación de las causas más frecuentes de ingreso a la Unidad de Cuidados Intensivos Pediátricos, tales como enfermedades infecciosas y generales. Se establecieron las enfermedades que incidían en una alta mortalidad en dicha Institución, como se representó en la relación entre causa de ingreso y egreso, observándose mayor mortalidad en las enfermedades infecciosas...
Assuntos
Criança , Cuidados Críticos , Epidemiologia , Morbidade , Pediatria , MortalidadeRESUMO
Objetivo: Determinar el rol de la irrigación con soluciones de preservación, la reperfusión y el rechazo en la apoptosis pulmonar en un escenario de trasplante pulmonar. Material y método: Venticuatro cerdos Landrace con un peso de 15 a 30 kilogramos fueron usados como donantes y receptores en un modelo de trasplante pulmonar izquierdo, con 5 días de sobrevida. Las muestras se obtuvieron en la siguiente secuencia: 1A: Donante, pulmón izquierdo inmediatamente luego de la apertura del torax. 1B: donante, pulmón derecho, inmediatamente luego de la apertura del tórax. 2A: Donante pulmón izquierdo, inmediatamente luego de la irrigación del organo. 2B Donante, pulmón derecho, sin irrigar. 3A: Pulmón izquierdo implantado, 1 hora luego de reperfundido en el receptor . 3B: Pulmón derecho (nativo), 1 hora luego de reperfundido el pulmón donante en el receptor. 4A: Pulmón izquierdo, biopsia transbronquial a las 48 horas postrasplante. 4B: Pulmón derecho, biopsia transbronquial a las 48 horas postransplante. 5A: Púlmón izquierdo, 5º día postrasplante (sacrificio). Todos los pulmones fueron irrigados con solución de Euro-Collins fría (4Cº) durante la ablación. Seis receptores no recibieron inmunosupresión y otros 6 receptores recibieron 15 mg/KG/ día de ciclosporina intravenosa.
Assuntos
Animais , Apoptose , Transplante de Pulmão/efeitos adversos , Transplante de Pulmão/estatística & dados numéricos , Suínos , Proliferação de Células , Reperfusão/estatística & dados numéricos , Lavagem Broncoalveolar/estatística & dados numéricos , Estatística como AssuntoRESUMO
Objetivo: Determinar el rol de la irrigación con soluciones de preservación, la reperfusión y el rechazo en la apoptosis pulmonar en un escenario de trasplante pulmonar. Material y método: Venticuatro cerdos Landrace con un peso de 15 a 30 kilogramos fueron usados como donantes y receptores en un modelo de trasplante pulmonar izquierdo, con 5 días de sobrevida. Las muestras se obtuvieron en la siguiente secuencia: 1A: Donante, pulmón izquierdo inmediatamente luego de la apertura del torax. 1B: donante, pulmón derecho, inmediatamente luego de la apertura del tórax. 2A: Donante pulmón izquierdo, inmediatamente luego de la irrigación del organo. 2B Donante, pulmón derecho, sin irrigar. 3A: Pulmón izquierdo implantado, 1 hora luego de reperfundido en el receptor . 3B: Pulmón derecho (nativo), 1 hora luego de reperfundido el pulmón donante en el receptor. 4A: Pulmón izquierdo, biopsia transbronquial a las 48 horas postrasplante. 4B: Pulmón derecho, biopsia transbronquial a las 48 horas postransplante. 5A: Púlmón izquierdo, 5º día postrasplante (sacrificio). Todos los pulmones fueron irrigados con solución de Euro-Collins fría (4Cº) durante la ablación. Seis receptores no recibieron inmunosupresión y otros 6 receptores recibieron 15 mg/KG/ día de ciclosporina intravenosa. Los niveles plasmáticos de ciclosporina fueron dosados en tiempo 0 al 2º y 5º día postrasplante. Cada muestra fue analizada por un observador ciego para determinar el grado de rechazo (A0 y A1 negativo. A2. A3 y A4 positivo), proliferación celular, y el índice de apaptosis en neumonocitos I y II empleando la técnica de TUNEL y Caspasa. Las pruebas de Chi cuadrado; prueba de t de student y kruskal Wallis fueron utilizadas para el análisis estadístico. Se consideró significativo un valor de p menor a 0.05. Resultados: El grado de rechazo fue negativo en todas las muestras excepto en 4A (1 animal) y 5A (5 animales sin ciclosporina y 3 animales de los que recibieron ciclosporina) (p<0.05)
Assuntos
Animais , Apoptose , Lavagem Broncoalveolar , Suínos , Proliferação de Células , Reperfusão , Transplante de Pulmão/efeitos adversos , Transplante de Pulmão , Estatística como AssuntoRESUMO
International rice export markets are increasing demands for rapid improvements in grain quality characteristics. The African rice Oryza glaberrima is a new potential source of genes that will enhance the eating, cooking, and milling properties of the rice grain. The objective of this research was to identify and characterize quantitative trait loci (QTLs) among 312 doubled haploid lines derived from the BC3F1 of an interspecific cross of O. sativa x O. glaberrima. Genetic material was planted in replicated plots and evaluated for ten grain quality traits in 2001 in Colombia. A linkage map was constructed with 100 polymorphic microsatellite markers using the mapdisto software program to adjust for segregation distortion. Transgressive segregation was observed for all traits. Interval and composite interval analyses identified 27 QTLs for nine characters located on 11/12 chromosomes. The chromosomal positions of QTLs for percentage amylose, alkali-spreading score, and percentage protein were in agreement with data reported by others, whereas QTL markers for percentage head rice, percentage milled rice, percentage protein, and percentage brown rice were different in our mapping population. Five major QTLs were found to be associated with improved percentage rice bran, percentage amylose, and alkali-spreading score. Seven QTLs for improved percentage rice bran, percentage milled rice, alkali-spreading score, percentage protein, and grain length/width ratio were derived from the O. glaberrima accession. Three new QTLs for percentage rice bran are reported here for the first time. Results from this study suggest that the African rice might be a valuable new source for introgression and improvement of several traits that affect quality traits demanded by the different rice export markets.
Assuntos
Hibridização Genética , Oryza/genética , Fenótipo , Locos de Características Quantitativas/genética , Sementes/fisiologia , Mapeamento Cromossômico , Colômbia , Repetições de Microssatélites/genética , Sementes/genéticaRESUMO
In neurocysticercosis, the nervous tissue surrounding the brain lesion is affected as a consequence of the local immune response induced by a Taenia solium metacestode. In this study, a histological and immunohistochemical analysis of five brain specimens from patients with neurocysticercosis revealed a proinflammatory activity reflected by an apparently altered blood-brain barrier permeability, secretion of pro-inflammatory cytokines, and up-regulation of molecules associated with antigen presentation. There were also anti-inflammatory cytokines, as well as an active wound-healing process reflected by angiogenesis, collagen deposition and glial scar formation. This immune response displayed by the nervous tissue adjacent to chronic neurocysticercosis lesions appeared to be contributing to the local tissue damage, and hence, may be fundamental in the pathology of NCC.
Assuntos
Neurocisticercose/imunologia , Neurocisticercose/patologia , Taenia/imunologia , Animais , Astrócitos/parasitologia , Barreira Hematoencefálica/imunologia , Química Encefálica/imunologia , Citocinas/análise , Fator 2 de Crescimento de Fibroblastos/análise , Humanos , Macrófagos/parasitologia , Mastócitos/patologia , Microglia/parasitologia , Células Th1/imunologia , Células Th2/imunologiaRESUMO
Of the 300 clinical files of pregnant women, 22 were associated with cancer plus pregnancy in 5 years. Of them 7 were excluded in the work. 15 patients with CaCu and pregnancy were studied in the Gineco-Obstetric Hospital No. 3 C.M. La Raza of the IMSS México City in a period since 1st of January 1988 to 31st December of 1992. Early menarchia and sexual life, take an important roll in the mexican women as predisponent factors, multideliver and tobacco too. The colposcopy with manage cervix biopsy is the election method of diagnosis, with 99.5% of confiability. In the 15 patients, the estirpe was epidermoid cancer in all of them. The diagnosis of pregnancy age made in the first and third trimester of gestation. Only in 3 patients (20%) were founded visible lesion, this rename the necessity of make detection of CaCu during the pregnancy, because we will can found the cases in preinvader stages or early invader, that cure highly, like the cases seen in this work. Stages preinvader and early invaders during the pregnancy are highly curatives. We concluded that they have worst prognosis of survival, aggressivity and early relapse in patients with pregnancy stage by stage. The last because in the Historical Literature review about this topic, there was statistical relevance about pregnancy in the women with CaCu, using our work for reference. CaCu does change his prognosis associated with the pregnancy.