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From 2006 through 2014, we conducted seroepidemiological surveys on non-human primates and sloths to investigate the possible circulation of arboviruses in Bahia Atlantic Forest, Brazil. We collected a total of 196 samples from 103 Leontopithecus chrysomelas, 7 Sapajus xanthosternos, 22 Bradypus torquatus and 7 Bradypus variegatus. Serum samples were tested using neutralization test and hemagglutination inhibition test to detect total antibodies against 26 different arboviruses. The overall prevalence of arboviruses was 36.6% (51/139), with the genus Flavivirus having the highest prevalence (33.1%; 46/139), followed by Phlebovirus (5.0%; 7/139), Orthobunyavirus (4.3%; 6/139) and Alphavirus (0.7%; 1/139). Monotypic reactions suggest that the wild animals were exposed naturally to at least twelve arboviruses. Added results from the neutralization test, animals were exposed to thirteen arboviruses. Most of these viruses are maintained in transmission cycles independent of human hosts, although antibodies against dengue virus serotypes 1, 2 and 3 were found in this study. To our knowledge, this is the first study reporting exposure to arboviruses in L. chrysomelas, S. xanthosternos and B. torquatus. Our results also highlight that the Southern Bahia Atlantic Forest has a variety of vertebrate hosts and potential vectors, which may support the emergence or re-emergence of arboviruses, including those pathogenic to humans.

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CONTEXTUALIZAÇÃO: O teste sentar e alcançar (TSA), utilizado para mensurar flexibilidade da coluna lombar e músculos isquiotibiais (IT), é mais adequado quando realizado concomitante à mensuração do ângulo da articulação do quadril (AAQ). OBJETIVO: Avaliar a confiabilidade intra e interobservadores do TSA na mensuração do comprimento dos IT por meio da análise cinemática angular. MÉTODO: Participaram 50 universitários (X= 21,5 anos; DP= 1,5) sem alterações musculoesqueléticas. Foram utilizados: banco padrão do TSA com porta para avaliar a ação dos gastrocnêmios no TSA e câmera fotográfica digital posicionada sobre um tripé. Marcadores cutâneos foram posicionados na: espinha ilíaca ântero-superior e trocânter maior. Realizaram-se duas aquisições de imagem: uma com porta fechada - PF e outra, aberta - PA. Para testar a confiabilidade intra e interobservadores foram utilizados: coeficiente de correlação intraclasse (CCI) - efeito aleatório com um fator - e teste de concordância de Bland e Altman (d = diferença média entre as medidas) com os respectivos intervalos de confiança de 95 por cento (IC 95 por cento). RESULTADOS: Os testes de confiabilidade intra-observador do AAQ foram: CCIpf = 0,97 - IC95 por cento [0,95;0,99] e Bland e Altman d = -0,02 - IC95 por cento [-0,11;0,07]; CCIpa= 0,98 - IC95 por cento [0,96;0,99], d = -0,01 - IC95 por cento [-0,11;0,08]. Para confiabilidade interobservador: CCIpf =0,96 - IC95 por cento [0,94;0,98] e d = -0,07 - IC95 por cento [-0,19;0,03]; CCIpa=0,96 - IC95 por cento [0,93;0,98] e d = -0,06 - IC95 por cento [-0,19;0,52]. CONCLUSÃO: Os testes intra e interobservador (PF e PA) da avaliação do AAQ, pela análise cinemática, apresentaram alta confiabilidade. A técnica é de fácil aplicação (necessita apenas do banco TSA padrão e câmera fotográfica) e fornece à prática clínica um método confiável para mensurar os IT pela cinemetria.

BACKGROUND: The sit-and-reach test (SRT) is used to measure the flexibility of the lumbar spine and hamstring muscles and is better when the hip joint angle (HJA) is measured concomitantly. OBJECTIVE: To assess the intra and interobserver reliability of the SRT for measuring hamstring muscle length through angular kinematic analysis. METHOD: Fifty university students (X= 21.5 years; SD= 1.5) without musculoskeletal abnormalities took part. A standard SRT bench with a door (to assess the action of the gastrocnemius muscles) and a digital photographic camera positioned on a tripod were used. Skin markers were placed on the anterosuperior iliac spine and greater trochanter. Two images were recorded: one with the door closed (DC) and another with the door open (DO). To test the intra and interobserver reliability, the intraclass correlation coefficient (ICC) (random effect with one factor) and the Bland and Altman concordance test (d= mean difference between measurements) were used, with the respective 95 percent confidence intervals (95 percent CI). RESULTS: The intraobserver reliability of the HJA test was: ICCdc= 0.97 (95 percent CI [0.95;0.99]) and Bland and Altman d = -0.02 (95 percent CI [-0.11;0.07]); ICCdo = 0.98 (95 percent CI [0.96;0.99]), d = -0.01 (95 percent CI [-0.11;0.08]). For the interobserver reliability: ICCdc= 0.96 (95 percent CI [0.94;0.98]) and d = -0.07 (95 percent CI [-0.19;0.03]); ICCdo= 0.96 (95 percent CI [0.93;0.98] and d = -0.06 (95 percent CI [-0.19;0.52]. CONCLUSION: The intra and interobserver reliability tests (DC and DO) for HJA assessment using kinematic analysis showed high reliability. The technique is easy to apply (only requiring a standard SRT bench and a photographic camera) and provides a reliable method for measuring hamstring muscles using kinematic analysis in clinical practice.

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Water mite (Krendowskia sp.) predation on zooplanktonic species from Lake Monte Alegre (Säo Paulo State, Brazil) was tested in laboratory experiments. One cladoceran, Daphnia gessneri, two copepods, Tropocyclops prasinus and Thermocyclops decipiens, and Chaoborus larvae III and IV were supplied as prey to adult mites. The cladoceran Ceriodaphnia cornuta was used in the experiment with mite nymphs. Only D.gessneri was preyed on by mites, at an average rate of 7 ind./mite/day, at 23-24ºC. Although Chaoborus was not eaten by the predator in the experiment, in direct observations older larvae were seen to be seized by several mites, both predators and prey sinking to the bottom of the vessel. Adult Daphnia was grabbed by its dorsal side, swimming together with the mite. There was an increase in predatory activity two hours after sunset

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Water mite (Krendowskia sp.) predation on zooplanktonic species from Lake Monte Alegre (São Paulo State, Brazil) was tested in laboratory experiments. One cladoceran, Daphnia gessneri, two copepods, Tropocyclops prasinus and Thermocyclops decipiens, and Chaoborus larvae III and IV were supplied as prey to adult mites. The cladoceran Ceriodaphnia cornuta was used in the experiment with mite nymphs. Only D. gessneri was preyed on by mites, at an average rate of 7 ind./mite/day, at 23-24 degrees C. Although Chaoborus was not eaten by the predator in the experiment, in direct observations older larvae were seen to be seized by several mites, both predators and prey sinking to the bottom of the vessel. Adult Daphnia was grabbed by its dorsal side, swimming together with the mite. There was an increase in predatory activity two hours after sunset.

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Water mite (Krendowskia sp.) predation on zooplanktonic species from Lake Monte Alegre (São Paulo State, Brazil) was tested in laboratory experiments. One cladoceran, Daphnia gessneri, two copepods, Tropocyclops prasinus and Thermocyclops decipiens, and Chaoborus larvae III and IV were supplied as prey to adult mites. The cladoceran Ceriodaphnia cornuta was used in the experiment with mite nymphs. Only D.gessneri was preyed on by mites, at an average rate of 7 ind./mite/day, at 23-24ºC. Although Chaoborus was not eaten by the predator in the experiment, in direct observations older larvae were seen to be seized by several mites, both predators and prey sinking to the bottom of the vessel. Adult Daphnia was grabbed by its dorsal side, swimming together with the mite. There was an increase in predatory activity two hours after sunset.

A predação do zooplâncton por ácaros aquáticos (Krendowskia sp.) do Lago Monte Alegre (Estado de São Paulo, Brasil) foi testada em experimentos de laboratório. Um cladócero, Daphnia gessneri, dois copépodos, Tropocyclops prasinus e Thermocyclops decipiens e larvas III e IV de Chaoborus foram oferecidos como presas a ácaros adultos. O cladócero Ceriodaphnia cornuta foi usado em experimentos com ninfas de ácaros. Apenas D. gessneri foi predada pelos ácaros, a uma taxa média de 7 ind./ácaro/dia, a 23-24ºC. Embora Chaoborus não tenha sido comido pelo predador no experimento, nas observações diretas, grupos de ácaros foram vistos agarrados a larvas III e IV. Ao agarrarem os caoborídeos, os ácaros paravam de nadar e afundavam com a presa. Ainda nas observações diretas, os ácaros foram vistos agarrados ao dorso de Daphnia adultas e, nesta ocasião, continuavam nadando com a presa. Foi observado aumento na atividade predatória duas horas após o pôr-do-sol.

RESUMO

Water mite (Krendowskia sp.) predation on zooplanktonic species from Lake Monte Alegre (São Paulo State, Brazil) was tested in laboratory experiments. One cladoceran, Daphnia gessneri, two copepods, Tropocyclops prasinus and Thermocyclops decipiens, and Chaoborus larvae III and IV were supplied as prey to adult mites. The cladoceran Ceriodaphnia cornuta was used in the experiment with mite nymphs. Only D.gessneri was preyed on by mites, at an average rate of 7 ind./mite/day, at 23-24ºC. Although Chaoborus was not eaten by the predator in the experiment, in direct observations older larvae were seen to be seized by several mites, both predators and prey sinking to the bottom of the vessel. Adult Daphnia was grabbed by its dorsal side, swimming together with the mite. There was an increase in predatory activity two hours after sunset.

A predação do zooplâncton por ácaros aquáticos (Krendowskia sp.) do Lago Monte Alegre (Estado de São Paulo, Brasil) foi testada em experimentos de laboratório. Um cladócero, Daphnia gessneri, dois copépodos, Tropocyclops prasinus e Thermocyclops decipiens e larvas III e IV de Chaoborus foram oferecidos como presas a ácaros adultos. O cladócero Ceriodaphnia cornuta foi usado em experimentos com ninfas de ácaros. Apenas D. gessneri foi predada pelos ácaros, a uma taxa média de 7 ind./ácaro/dia, a 23-24ºC. Embora Chaoborus não tenha sido comido pelo predador no experimento, nas observações diretas, grupos de ácaros foram vistos agarrados a larvas III e IV. Ao agarrarem os caoborídeos, os ácaros paravam de nadar e afundavam com a presa. Ainda nas observações diretas, os ácaros foram vistos agarrados ao dorso de Daphnia adultas e, nesta ocasião, continuavam nadando com a presa. Foi observado aumento na atividade predatória duas horas após o pôr-do-sol.

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Based on occupational classification of AIDS cases reported in Brazil in 1995 and recorded in the Data Base for Reported Diseases (SINAN), AIDS incidence rates were estimated per 100 thousand inhabitants by occupation, according to the 1991 National Census. Nationwide rates for employed males and females were 25.0 and 9.1, respectively. Among males, and within the major occupational groups, the following sub-groups had the highest rates: personal hygiene services (268.1), social scientists (176.1), writers and journalists (114.3), medical and dental assistants (113.4), chemists, pharmacists, and physicists (111.9), teachers (87.3), artists (74.7), port services (65.6), maritime and fluvial transportation (57.5), and sales personnel (55.4). The results indicated the disease's magnitude in different occupational categories and highlighted certain occupations in which special preventive measures are justified, along with more in-depth studies focusing on other socioeconomic variables and the sociocultural behavior of their members.

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