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The effects of ß-mannanase supplementation in metabolizable energy (ME)-reduced diets containing xylanase-phytase were investigated on growth performance, fecal score, ultra-sounded backfat thickness and loin depth, blood profile, apparent total tract digestibility (ATTD), digesta passage rate, and fecal microbiome in grower pigs (n = 40, 26.09 ± 0.96 kg) randomly assigned within 4 treatments: a control diet containing isolated phytase and xylanase valued at 40 kcal of ME/kg (CD0), CD0 + ß-mannanase (0.3 g/kg valued at 30 kcal of ME/kg) (CD70), CD0 + ß-mannanase (0.3 g/kg valued at 45 kcal of ME/kg) (CD85), and CD0 + ß-mannanase (0.3 g/kg valued at 60 kcal of ME/kg) (CD100). Growth performance was not affected in pigs fed ME-reduced diets containing ß-mannanase. Pigs with CD100 had lower serum IL-1ß concentration, and higher IL-10 was observed in pigs on CD0 than those fed ß-mannanase. Coefficients of ATTD, and ATTD of DM and CP were higher in animals fed CD85 or CD100. Pigs with CD85 had higher alpha diversity richness but lower Firmicutes:Bacteroidota ratio. Acidaminococcaceae and Ruminococcaceae were more abundant in pigs fed CD0, but lower for Christensenellaceae NSJ-63 and NSJ-63 sp014384805. Pigs in CD85 showed higher Bacteroidaceae and Prevotella abundance, and lower for Streptococcaceae and Streptococcus. In conclusion, supplementation of ß-mannanase in diets containing xylanase-phytase saved 85 to 100 kcal of ME/kg by supporting growth performance and improving nutrient digestibility in grower pigs.

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This study aimed to assess the effects of ß-mannanase supplementation in metabolizable energy (ME)-reduced diets containing xylanase-phytase on performance, fecal score, blood biochemical and immunological profile, apparent total tract digestibility (ATTD), digesta passage rate, fecal microbiome, carcass traits and meat quality in finisher pigs (n = 40 entire male hybrid, 26.0 ± 0.9 kg) randomly assigned to 1 of 4 dietary treatments: a control diet containing isolated phytase and xylanase valued at 40 kcal of ME/kg (CD0), CD0 + ß-mannanase (0.3 g/kg valued at 30 kcal of ME/kg) (CD70), CD0 + ß-mannanase (0.3 g/kg valued at 45 kcal of ME/kg) (CD85), and CD0 + ß-mannanase (0.3 g/kg valued at 60 kcal of ME/kg) (CD100), with 10 pen replicates. Pigs fed CD0 diet showed (P = 0.002) greater ADFI. However, pigs fed CD0 diet showed (P = 0.009) lower G:F than those provided CD70 or CD85 diets. A greater (P < 0.001) superoxide dismutase concentration was observed in pigs fed CD70 diet. Pigs fed CD85 diet showed (P = 0.002) greater digestible protein than pigs fed CD0 or CD100 diets. Pigs fed CD70 diet showed an increase of 11.3% in digestible protein than those fed CD0 diet. In addition, greater (P < 0.001) digestible energy was observed in pigs fed CD85 diet. Pigs fed CD0 or CD100 diets showed greater (P < 0.05) Firmicutes:Bacteroidota ratio than those fed CD85 diet. The Muribaculaceae was more abundant (P = 0.030) in pigs fed CD70 diet than in those fed CD0 diet. The Prevotella was more abundant (P = 0.045) in pigs fed CD85 diet than in those fed CD100 diet. In conclusion, ß-mannanase supplementation in diets containing xylanase-phytase allows reducing 85 kcal of ME/kg because it improves gain to feed ratio, energy and protein usage, and backfat thickness without metabolic and intestinal ecosystem disorders in finisher pigs.

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The quality of poultry by-product meal (PBM) is not standardized in the industry. Several factors are detrimental to PBM and compromise its nutritional value and shelf life. Therefore, this study was conducted to determine the main PBM production factors that directly affect its in vitro organic matter digestibility (IVDOM) and protein oxidation (POX). Data on the processing of PBM samples (n = 100) were recorded in a rendering plant. Two types of PBM were used: 1) Low ash (LA, n = 66) with mineral matter (MM) content of 11% and 2) High ash (HA, n = 34) with MM above 11%. Processing traits and chemical composition of PBM were considered independent variables. The IVDOM and POX were determined in each sample and considered dependent variables. Data on independent variables were submitted to factorial and principal components (PC) analyses. In vitro organic matter digestibility data were clustered (P = 0.001) in low (778.92 g/kg), average (822.85 g/kg), and high (890.06 g/kg). The best arrangement was composed of six independent variables distributed in two PC, which explained 82.10% of the total variation. The ash concentration, oil to raw material ratio, collagen, and crude protein comprised PC1 with greater relevance and explained 58.46% of the total variance. The PC2 was composed of the processing time and temperature and explained 23.64% of the total variance. Protein oxidation data were clustered (P < 0.001) in low (265.19 nmol/mg CP), average (393.07 nmol/mg CP), and high (524.40 nmol/mg CP). Based on our results, the composition of the raw material from the slaughterhouse holds most of the information on PBM composition and digestibility. Developing improvements in the slaughtering or in the screening of the raw material that will be used by the rendering process is important to obtain a more nutritionally standardized ingredient.

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BACKGROUND: Homeopathic products (HP) have been proposed for the prevention of disease and as a complementary dietary additive in pig farming, with resulting improved performance and quality of life of the animals and with benefits for food safety. The aim of this study was to assess the addition of HP to pig feed and its effects on growth performance, dietary protein utilization, nutrient digestibility, meat quality, and economic feasibility. METHODS: A total of 80 male pigs, body weight 32.8±2.3kg, were allocated in a randomized design, with two treatments of 10 replications and four animals per experimental unit. Treatments were composed of a control diet (CD) or CD+HP (Finalvitaplus+Figotonus, 1.1kg/ton feed of each product). The outcome measures used were growth performance (body weight gain) and feed efficiency (body weight gain per unit of feed consumed), efficiency of dietary protein utilization (blood urea nitrogen), nutrient digestibility (apparent digestibility coefficient and apparent nutrient digestibility), meat quality (including pH, temperature, color, liquid loss by cooking and thawing, intramuscular fat and meat tenderness), and economic feasibility (cost of the diets). RESULTS: There was no effect (p >0.05) of treatments on growth performance of the pigs, though the group that received HP showed an 8.93% greater feed efficiency than the control group (p=0.077). The animals in the control group had a 14.37% higher blood urea nitrogen concentration in the finishing I phase (70-100kg body weight) compared with those fed HP (p=0.028), indicating lower protein utilization in the controls. There was comparative improvement in digestibility coefficient and in nutrient digestibility in pigs fed HP in the growing-finishing phase (p <0.05). There was no differential effect of treatments on meat quality or economic feasibility (p >0.05). CONCLUSION: The addition of HP to the diet of growing-finishing pigs produced some improvement in their dietary protein utilization, nutrient digestibility and feed efficiency, though it did not affect their growth performance, meat quality or economic feasibility.

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OBJECTIVE: This study was to assess the effects of different doses of an essential oil blend (EOB) on growth performance, diarrhea occurrence (DO), hematological and blood biochemical profile, intestinal morphometry, morphology and microbiology, relative weight and length of organs, digestive content pH, and liver antioxidant status in weaning piglets. METHODS: A total of 135 barrows (7.09±0.29 kg body weight) were allotted randomly in a randomized complete block design based on body weight with nine replications and three animals per pen. Dietary treatments were a negative control (NC): basal diet; positive control (PC): NC plus 125 mg performance-enhancing antibiotic (enramycin 8%)/kg diet; NC plus 100 mg EOB/kg diet (EO100); NC plus 200 mg EOB/kg diet (EO200); and NC plus 400 mg EOB/kg diet (EO400). Diarrhea occurrence was monitored daily, and performance at the end of each phase. RESULTS: Gain to feed ratio was greater (p<0.05) in starter II pigs fed EO400 and EO200 than in those fed EO100. Pigs fed EO400 had lower (p<0.05) DO than those fed NC and EO100 in the total period. Pre-starter II pigs fed NC had (p<0.05) lower serum total protein and plasma protein than pigs fed PC. Pigs fed EO100 showed smaller (p<0.05) mean corpuscular volume (MCV) than pigs fed EO400. Starter II pigs fed EO400 had (p<0.05) greater MCV and lower mean corpuscular hemoglobin and erythrocytes than those fed EO100. There was a greater concentration (p<0.05) of band cells for PC, similar to EO400 and EO200. Performance-enhancing antibiotic and EOB to diets increased (p<0.05) liver superoxide dismutase activity. CONCLUSION: Adding 200 and 400 mg EOB/kg diet decreased DO and was advantageous to hematological and blood biochemical profile and liver antioxidant status without being detrimental to growth performance and gastrointestinal health in nursery pigs.

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The study aimed to measure variations in industrial process and nutritional variables of poultry by-product meal (PBM) in rendering plants from batch cookers. A total of 200 samples of low ash PBM with mineral matter (MM) content of 11% (LA, n = 104) or high ash with MM above 11% (HA, n = 96) were collected from 5 industrial processing plants. The highest coefficients of variation in chemical composition were for MM (LA - 19.70%; HA - 19.59%), ether extract (LA - 20.72%; HA - 14.86%), collagen (LA - 21.16%; HA - 30.00%) and water activity (LA - 24.54%; HA - 25.89%). However, the crude protein (LA - 5.07%; HA - 7.39%), dry matter (LA - 1.75%; HA - 2.90%) and organic matter digestibility (LA - 4.81%; HA - 6.78%) were lower. The variability of the data related to the process of PBM was: maximum process temperature (LA - 3.91%; HA - 3.56%), average process temperature (LA - 3.73%; HA - 4.71%), and processing time (LA - 27.37%; HA - 37.59%). This study evidenced that the corrective measures by limiting the amount of bones in the raw material, optimizing the pressing step for the poultry fat extraction, and also controlling the processing time of PBM may favor the production of more standardized PBM in terms of chemical composition and quality.

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This study was conducted to assess the effect of two types of drinkers on water intake (WI) and two types of toys on behavioural observations of piglets in the nursery phase. A total of 72 crossbred entire male piglets (Landrace × Large White, Agroceres♂ and DanBred♀), weaned at 21 days of age, with an average initial body weight (AIBW) of 6.75 ± 0.19 kg were used. Two statistical models were fitted for WI. In model I, animals were distributed in a completely randomized design (CRD) with a split-plot in time, composed of six treatments (2 types of drinkers × 3 experimental phases), eight replications (days of sampling), and 36 animals/experimental unit (EU), without using AIBW as a covariate. In model II, animals were distributed in a CRD with two treatments (fixed or pendular nipple drinkers), eight replications (days of sampling) and 36 animals/EU, with use of AIBW as a covariate. For assessment of behavioural observations, the model was adjusted in a randomized block design, consisting of four treatments [two types of toys (metal chain and plastic bottle) × two daytime periods (morning and afternoon)], nine replications and three blocks constituted in time (days), totalling four pigs/ EU. The results indicated an effect on animal daily WI in the starter phase in model I (p < 0.000) and II (p = 0.006). There was an effect (p ≤ 0.05) of toy type for behavioural observations, in which pigs showed a greater proportion of fighting and belly nosing when they had the bottle toy. However, animals spent more time (p ≤ 0.05) eating, drinking water, overlapping, and playing with the toy when they had access to the chain toy. There was an increase (p ≤ 0.05) in sleeping behaviour in the morning period, as well as greater (p ≤ 0.05) behavioural activity in the afternoon period. In conclusion, WI of nursery piglets was positively influenced by growth phase, and the fixed drinker stimulated greater WI in piglets in the starter phase. In addition, the metal chain, as an environmental enrichment, promoted improvements in feeding behaviour and WI, reducing fighting events, and the afternoon period had increased behavioural observations overall.(AU)

Um estudo foi conduzido com o objetivo de avaliar dois tipos de bebedouros e seus efeitos sobre o consumo hídrico (CH) e dois tipos de brinquedos sobre os eventos comportamentais de leitões na fase de creche. Um total de 72 leitões machos inteiros mestiços (Landrace × Large White, Agroceres♂ e DanBred♀), desmamados aos 21 dias de idade e peso corporal inicial médio (PCIM) de 6,75 ± 0,19 kg. Para o CH foram ajustados dois modelos estatísticos. No modelo I, os animais foram distribuídos em um delineamento inteiramente casualizado (DIC) e esquema de parcelas subdivididas no tempo, composto de seis tratamentos (2 tipos de bebedouros × 3 fases experimentais), oito repetições (dias de amostragem) e 36 animais/unidade experimental (UE) e sem utilização de PCIM como covariável. No modelo II, os animais foram distribuídos em um DIC aninhado com dois tratamentos (bebedouro tipo chupeta fixo ou pendular), oito repetições (dias de amostragem) e 36 animais/UE, com utilização de PCIM como covariável. Para a avaliação dos eventos comportamentais, foi ajustado um modelo em delineamento de blocos casualizados, constituído de quatro tratamentos [dois tipos de brinquedos (corrente de metal e garrafa plástica) × dois períodos do dia (matutino e vespertino)], nove repetições e três blocos constituídos no tempo (dias), totalizando quatro suínos/UE. Os resultados indicaram efeito no CH diário animal na fase inicial no modelo I (p < 0,000) e II (p = 0,006). Houve efeito (p ≤ 0,05) de tipo de brinquedo para as observações comportamentais, em que os suínos apresentaram maior proporção de brigas e empurrando a barriga quando tiveram o brinquedo de garrafa. Entretanto, os animais passaram maior tempo (p ≤ 0,05) comendo, bebendo água, montando outro animal e brincando com o brinquedo quando tiveram acesso ao brinquedo de corrente. Houve um aumento (p ≤ 0,05) no comportamento de dormir no período matutino, bem como os suínos apresentaram maior (p ≤ 0,05) atividade comportamental no período vespertino. Em conclusão, o consumo hídrico de leitões em fase de creche é influenciado positivamente pela fase de crescimento e o bebedouro fixo estimulou um maior consumo de água em leitões na fase inicial. Em adição, o tipo de enriquecimento ambiental corrente promoveu melhorias no comportamento alimentar e de ingestão de água, reduzindo eventos de brigas, bem como o período vespertino aumentou os eventos comportamentais em geral.(AU)

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The aim of this study was to assess the addition of intestinal alkaline phosphatase (IAP) to diets on the count of bacterial populations, pH of digestive organ contents, histopathological description, proinflammatory markers, hepatic glycogen reserve, and diarrhoea incidence of piglets challenged with Escherichia coli. Sixty-four crossbred piglets (7.16±0.28 kg body weight, 25-days-old) were assigned to four treatments in a completely randomised block design: negative control (NC), NC + antibiotic (ANT), NC + 15 mg IAP, or NC + 30 mg IAP kg−1 of diet, eight replications of two piglets per experimental unit. All piglets were orally challenged with 6 mL of a solution containing enterotoxigenic Escherichia coli K88 at 106 CFU mL−1 at 15 days of experimentation. The study lasted for 19 days. At the end of the experimental period, the piglets were slaughtered (six animals per treatment). Enterobacteriaceae in caecum and colon was lower in piglets on 30 mg IAP than with ANT and NC, ANT or 15 mg IAP, respectively. Enterobacteriaceae adhered to the mesenteric lymph nodes (MLN) was greater in piglets fed ANT than the other treatments. Lactic acid bacteria (LAB) count in caecum was greater in piglets fed NC and ANT. In MLN, LAB count was greater in ANT and 30 mg IAP-fed piglets compared with 15 mg IAP. Piglets in 30 mg IAP in diet showed a tendency for lowering tissue necrosis compared with NC or ANT. Piglets fed 30 mg IAP showed a reduction in diarrhoea incidence in the pre- and post-challenge compared with 15 mg IAP and all other treatments, respectively. Based on the criteria, addition of 30 mg IAP to diet inhibits Enterobacteriaceae population and suggests a potential effect in mitigating intestinal injuries, as observed in piglets in the NC for some of the parameters investigated.(AU)

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This study was conducted to assess the effects of maternal dietary calcitic seaweed (CSW) on performance and blood metabolites of sows, and on performance, blood metabolites, intestinal microbiota, and parameters of gastrointestinal tract and bone of litters. On d 21 (post-insemination), non-pregnant sows were removed from the trial, remaining 19 sows in control group (without CSW) and 16 sows receiving CSW. Then, a total of 35 sows were allocated in a randomized block design with two treatments: control diet with calcitic limestone plus dicalcium phosphate (CTL) or CTL plus 0.4% CSW. In gestation, sows were fed twice a day (07:00 and 15:00 h) to reach an intake of 2.5 kg animal−1 day−1 divided into two equal meals. On parturition day, sows were offered only 0.5 kg feed animal−1. Throughout lactation, sows were fed three times a day (≅7 kg animal−1 day−1). All diets were provided as mash. Results suggested that sows fed CTL had litters with lower body weight at birth compared with those fed CSW. Sows fed CSW had 14.28% more live-born piglets and lower stillborns. Piglets from sows fed CSW showed greater calcium concentration on d 14 after birth than those from sows fed CTL. Sows fed CSW showed better milk chemical composition and an increase of 27.16% in milk production compared with those fed CTL. Piglets from sows fed CSW had an increase in cecum content in the Enterobacteriaceae count. This study showed that adding 0.4% CSW in the diet of pregnant and lactating sows as an organic calcium source positively influences the number of live-born piglets and the percentage of stillborns. In addition, milk composition and production are also improved without affecting piglets' biological response.(AU)

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Three experiments were carried out to determine the nutritional value and the effect of acerola seed meal (ASM) for starter piglet diets on feed digestibility, nitrogen balance (NB), growth performance and blood metabolites. Twenty-four crossbred entire male piglets (21.07 ± 3.07 kg) were assigned to a digestibility assay (Exp. I) and distributed in a randomised block design (RBD) with two treatments composed of reference diet (RD) or test diet (20% replacement with ASM), 12 replications and with one pig per experimental unit (EU). An NB study (Exp. II) was conducted with four levels of ASM (0, 4, 8 and 12%) and 24 crossbred entire male piglets (20.78 ± 1.84 kg) allocated in an RBD of six replications and one pig per EU. The growth performance study (Exp. III) involved 120 crossbred piglets: 60 entire males and 60 females (13.85 ± 1.49 kg). Treatments consisted of a gender combination and five levels of ASM (0, 3, 6, 9 and 12%), distributed in an RBD with six replications and four animals per EU. The physicochemical composition results indicated 86.12% of dry matter (DM), 8.03% of crude protein (CP) and 4,553 kcal kg-1 of gross energy (GE) as well as apparent digestibility coefficients of 39.04 and 28.21% for CP and GE, respectively. The levels of ASM that provided the maximum estimate for final body weight, daily body weight gain and average daily feed intake were 2.93% (p = 0.037), 3.05% (p = 0.040) and 4.27% (p = 0.043), respectively. ASM can be indicated as a dietary fibre source for starter piglet diets because it contains relevant nutritional and energy values, without affecting the nitrogen balance and blood urea concentration.(AU)

Três experimentos foram realizados para determinar o valor nutricional e o efeito do uso do farelo da semente de acerola (FSA) na dieta de leitões em fase inicial sobre a digestibilidade de ração, balanço de nitrogênio (BN), desempenho zootécnico e metabólito sanguíneo. Vinte e quatro leitões mestiços, machos inteiros (21,07 ± 3,07 kg) foram atribuídos a um ensaio de digestibilidade (Exp. I.) e distribuídos em um delineamento experimental de bloco ao acaso (DBC), com dois tratamentos, compostos de uma dieta referência ou teste (substituição em 20% por FSA na dieta referência), 12 repetições e com um suíno por unidade experimental (UE). O ensaio de BN (Exp. II) foi conduzido com quatro níveis crescentes de inclusão de FSA (0, 4, 8 e 12%) e 24 leitões mestiços machos inteiros (20,78 ± 1,84 kg), alocados em um DBC de seis repetições e um suíno por UE. O estudo de desempenho zootécnico (Exp. III) envolveu 120 leitões mestiços, 60 machos inteiros e 60 fêmeas (13,85 ± 1,49 kg). Os tratamentos consistiram da combinação de dois gêneros e cinco níveis crescentes de inclusão de FSA (0, 3, 6, 9 e 12%), distribuídos em um DBC com seis repetições e quatro animais por UE. Os resultados de composição físico-química do FSA indicaram 86,12% de matéria seca (MS), 8,03% de proteína bruta (PB) e 4.553 kcal kg -1 de energia bruta (EB), e coeficientes de digestibilidade aparente de 39,04 e 28,21% para PB e EB, respectivamente. Os níveis de FSA que proporcionaram a máxima estimativa de peso corporal final, ganho de peso corporal diário (GPCDM) e consumo de ração diário médio foram 2,93% (p = 0,0372), 3,05% (p = 0,0407) e 4,27% (p = 0,0432), respectivamente. O FSA pode ser indicado como uma fonte de fibra dietética na alimentação de suínos em fase inicial por conter valores nutricionais e de energia relevantes sem afetar o balanço de nitrogênio e concentração de ureia no sangue.(AU)

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Balancing diets for protein affects both protein and energy use efficiency in cattle. This study aimed to evaluate the effects of dietary protein level on intake, digestibility and energy expenditure in Holstein heifers. Four Holstein heifers with a mean BW of 266.5 ± 10.7 kg were distributed in a 4 x 4 balanced Latin square design. Each period was comprised of 14 d for adaptation and 6 d for samplings. The heifers were fed diets with 9, 12, 15 and 18% of crude protein (CP). Total feces and urine were collected. Rumen fluid was collected at 2, 4, 6 and 8 h after feeding. The energy expenditure was evaluated at 6 a.m., 12 a.m., 6 p.m. and 12 p.m. by a closed-circuit mask technique. The intake of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) were similar between treatments (P > 0.05). Although the NDF digestibility was not changed (P > 0.05), the digestibilities of DM, OM, CP, non fibrous carbohydrates, and total digestible nutrients increased linearly (P < 0.05) with increasing CP levels in the diet. Urinary urea nitrogen excretion and nitrogen retained increased linearly (P < 0.01) with increasing CP levels. Dietary CP levels did not affect (P =0.53) energy expenditure in heifers. Ruminal ammonia-nitrogen concentration peaked at 2-4 h after feeding. Increasing dietary CP levels from 9 to 18% does not affect feed intake and energy expenditure in heifers. However, it improves the digestibility of OM and nitrogen retention.(AU)

O balanceamento das dietas proteicas afeta tanto a eficiência no uso de proteínas quanto a eficiência no uso de energia em bovinos. Este estudo teve como objetivo avaliar os efeitos dos níveis de proteína na dieta sobre consumo, digestibilidade e gasto de energia em novilhas Holandesas. Quatro novilhas Holandesas com um peso corporal (PC) médio de 266,5 ± 10,7 kg foram distribuídas em delineamento quadrado latino 4 x 4. Cada período era composto de 14 d para adaptação e 6 d para amostragens. As novilhas foram alimentadas com 9,12,15 e 18% de proteína bruta (PB). Fezes e urina totais foram coletadas. O líquido ruminal foi coletado às 2, 4, 6 e 8 h após a alimentação. O gasto de energia foi avaliado às 6h, 12h, 18h e 12h por uma técnica de máscara de circuito fechado. O consumo de matéria seca(MS), matéria orgânica (MO) e fibra em detergente neutro (FDN) foram similares entre os tratamentos(P > 0,05). Embora a digestibilidade do FDN não tenha sido alterada (P > 0,05), a digestibilidade de MS, MO, PB, carboidratos não-fibrosos e nutrientes digestíveis totais aumentou linearmente (P < 0,05)com o aumento dos níveis de PB na dieta. A excreção de uréia urinária e o nitrogênio retido aumentaram linearmente (P < 0,01). Os níveis de PB na dieta não afetaram (P = 0,53) o gasto de energia em novilhas. A concentração de nitrogênio amoniacal atingiu o pico em 2-4 h após a alimentação. O aumento dos níveis dietéticos de PB de 9 para 18% não afeta o consumo de ração e o gasto de energia em novilhas. Entretanto, melhora a digestibilidade da MO e a retenção de nitrogênio.(AU)

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Balancing diets for protein affects both protein and energy use efficiency in cattle. This study aimed to evaluate the effects of dietary protein level on intake, digestibility and energy expenditure in Holstein heifers. Four Holstein heifers with a mean BW of 266.5 ± 10.7 kg were distributed in a 4 x 4 balanced Latin square design. Each period was comprised of 14 d for adaptation and 6 d for samplings. The heifers were fed diets with 9, 12, 15 and 18% of crude protein (CP). Total feces and urine were collected. Rumen fluid was collected at 2, 4, 6 and 8 h after feeding. The energy expenditure was evaluated at 6 a.m., 12 a.m., 6 p.m. and 12 p.m. by a closed-circuit mask technique. The intake of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) were similar between treatments (P > 0.05). Although the NDF digestibility was not changed (P > 0.05), the digestibilities of DM, OM, CP, non fibrous carbohydrates, and total digestible nutrients increased linearly (P < 0.05) with increasing CP levels in the diet. Urinary urea nitrogen excretion and nitrogen retained increased linearly (P < 0.01) with increasing CP levels. Dietary CP levels did not affect (P =0.53) energy expenditure in heifers. Ruminal ammonia-nitrogen concentration peaked at 2-4 h after feeding. Increasing dietary CP levels from 9 to 18% does not affect feed intake and energy expenditure in heifers. However, it improves the digestibility of OM and nitrogen retention.

O balanceamento das dietas proteicas afeta tanto a eficiência no uso de proteínas quanto a eficiência no uso de energia em bovinos. Este estudo teve como objetivo avaliar os efeitos dos níveis de proteína na dieta sobre consumo, digestibilidade e gasto de energia em novilhas Holandesas. Quatro novilhas Holandesas com um peso corporal (PC) médio de 266,5 ± 10,7 kg foram distribuídas em delineamento quadrado latino 4 x 4. Cada período era composto de 14 d para adaptação e 6 d para amostragens. As novilhas foram alimentadas com 9,12,15 e 18% de proteína bruta (PB). Fezes e urina totais foram coletadas. O líquido ruminal foi coletado às 2, 4, 6 e 8 h após a alimentação. O gasto de energia foi avaliado às 6h, 12h, 18h e 12h por uma técnica de máscara de circuito fechado. O consumo de matéria seca(MS), matéria orgânica (MO) e fibra em detergente neutro (FDN) foram similares entre os tratamentos(P > 0,05). Embora a digestibilidade do FDN não tenha sido alterada (P > 0,05), a digestibilidade de MS, MO, PB, carboidratos não-fibrosos e nutrientes digestíveis totais aumentou linearmente (P < 0,05)com o aumento dos níveis de PB na dieta. A excreção de uréia urinária e o nitrogênio retido aumentaram linearmente (P < 0,01). Os níveis de PB na dieta não afetaram (P = 0,53) o gasto de energia em novilhas. A concentração de nitrogênio amoniacal atingiu o pico em 2-4 h após a alimentação. O aumento dos níveis dietéticos de PB de 9 para 18% não afeta o consumo de ração e o gasto de energia em novilhas. Entretanto, melhora a digestibilidade da MO e a retenção de nitrogênio.

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RESUMO

This study was conducted to evaluate the effects of including two types of crude glycerin (CG) in feed on plasma parameters, quantitative and qualitative carcass characteristics, and the fatty acid profile of the Longissimus dorsi pig muscle. Sixty-three commercial line pigs were used, with an average weight of 30.53 ± 1.59 to 59.55 ± 4.27 kg in the growing phase and 60.35 ± 2.25 to 89.91 ± 5.23 kg in the finishing phase. The pigs diet included two sources of CG (vegetable and mixed) at four inclusion levels (3%, 6%, 9% and 12%). One CG was derived from soybean oil (VCG) and the other from 80% animal fat + 20% soybean oil (MCG). The control diet contained 0% glycerin. Blood samples were collected at the beginning, middle and final phases of the trial. In addition, at the end of the finishing phase, the animals were fasted for 12 h and then sacrificed to measure the quantitative and qualitative carcass characteristics and the fatty acid profile of the Longissimus dorsi. The results indicate that in both phases there was a quadratic effect on plasma triglycerides. There was no effect on carcass parameters. A quadratic behaviour for pH 24h was obtained, with the lowest value attained at 1.05% CG inclusion. There was an interaction between the type of glycerin and the inclusion level in the diets for polyunsaturated fatty acids. VCG and MCG can be used at up to 12% inclusion because they cause no negative effects on carcass characteristics and pig meat quality.(AU)

Este estudo foi conduzido para avaliar os efeitos de inclusão de dois tipos de glicerina bruta (GB) sobre os parâmetros plasmáticos, características quantitativas e qualitativas da carcaça, e o perfil de ácido graxo do músculo Longissimus dorsi de suínos. Foram utilizados 63 suínos de linhagem comercial, com um peso médio de 30,53 ± 1,59 a 59,55 ± 4,27 kg na fase de crescimento e 60,35 ± 2,25 a 89,91 ± 5,23 kg na fase de terminação. A dieta dos suínos incluiu duas fontes de GB (Vegetal e Mista) com quatro níveis de inclusão (3, 6, 9 e 12%). Uma GB foi derivada de óleo de soja (GBV) e outra de 80% de gordura animal + 20% de óleo de soja (GBM). A dieta controle continha 0% de glicerina. Amostras de sangue foram coletadas nas fases inicial, intermediária e final do estudo. Além disso, ao final da fase de terminação, os animais permaneceram em jejum por 12 h e foram sacrificados para mensurar as características quantitativas e qualitativas da carcaça e o perfil de ácido graxo do Longissimus dorsi. Os resultados indicam em ambas as fases, houve um efeito quadrático nos triglicerídeos plasmáticos. Não houve efeito sobre os parâmetros de carcaça. Um comportamento quadrático para pH 24h foi obtido, com o menor valor atingido em 1,05% de inclusão de GB. Houve interação entre os tipos de glicerina e os níveis de inclusão nas dietas para ácidos graxos poli-insaturados. A GBV e a GBM podem ser usados em até 12% de inclusão porque não causam efeitos negativos nas características de carcaça e na qualidade da carne de suínos.(AU)

Assuntos

RESUMO

This study was conducted to evaluate the effects of including two types of crude glycerin (CG) in feed on plasma parameters, quantitative and qualitative carcass characteristics, and the fatty acid profile of the Longissimus dorsi pig muscle. Sixty-three commercial line pigs were used, with an average weight of 30.53 ± 1.59 to 59.55 ± 4.27 kg in the growing phase and 60.35 ± 2.25 to 89.91 ± 5.23 kg in the finishing phase. The pigs’ diet included two sources of CG (vegetable and mixed) at four inclusion levels (3%, 6%, 9% and 12%). One CG was derived from soybean oil (VCG) and the other from 80% animal fat + 20% soybean oil (MCG). The control diet contained 0% glycerin. Blood samples were collected at the beginning, middle and final phases of the trial. In addition, at the end of the finishing phase, the animals were fasted for 12 h and then sacrificed to measure the quantitative and qualitative carcass characteristics and the fatty acid profile of the Longissimus dorsi. The results indicate that in both phases there was a quadratic effect on plasma triglycerides. There was no effect on carcass parameters. A quadratic behaviour for pH 24h was obtained, with the lowest value attained at 1.05% CG inclusion. There was an interaction between the type of glycerin and the inclusion level in the diets for polyunsaturated fatty acids. VCG and MCG can be used at up to 12% inclusion because they cause no negative effects on carcass characteristics and pig meat quality.

Este estudo foi conduzido para avaliar os efeitos de inclusão de dois tipos de glicerina bruta (GB) sobre os parâmetros plasmáticos, características quantitativas e qualitativas da carcaça, e o perfil de ácido graxo do músculo Longissimus dorsi de suínos. Foram utilizados 63 suínos de linhagem comercial, com um peso médio de 30,53 ± 1,59 a 59,55 ± 4,27 kg na fase de crescimento e 60,35 ± 2,25 a 89,91 ± 5,23 kg na fase de terminação. A dieta dos suínos incluiu duas fontes de GB (Vegetal e Mista) com quatro níveis de inclusão (3, 6, 9 e 12%). Uma GB foi derivada de óleo de soja (GBV) e outra de 80% de gordura animal + 20% de óleo de soja (GBM). A dieta controle continha 0% de glicerina. Amostras de sangue foram coletadas nas fases inicial, intermediária e final do estudo. Além disso, ao final da fase de terminação, os animais permaneceram em jejum por 12 h e foram sacrificados para mensurar as características quantitativas e qualitativas da carcaça e o perfil de ácido graxo do Longissimus dorsi. Os resultados indicam em ambas as fases, houve um efeito quadrático nos triglicerídeos plasmáticos. Não houve efeito sobre os parâmetros de carcaça. Um comportamento quadrático para pH 24h foi obtido, com o menor valor atingido em 1,05% de inclusão de GB. Houve interação entre os tipos de glicerina e os níveis de inclusão nas dietas para ácidos graxos poli-insaturados. A GBV e a GBM podem ser usados em até 12% de inclusão porque não causam efeitos negativos nas características de carcaça e na qualidade da carne de suínos.

Assuntos

RESUMO

The objective was to test the hypothesis that ruminal inoculum obtained from slaughtered cattle can replace inoculum from cannulated cattle in trials evaluating animal feedstuffs through in vitro gas production and digestibility. Five adult Holstein × Zebu steers with ruminal cannula were used to collect and compare rumen liquid from in vivo and slaughtered animals. In vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD) and ammoniacal nitrogen concentrations did not differ between inoculum sources (slaughtered × cannulated) for all byproducts and levels tested. Total in vitro gas production in the ruminal inoculum of cannulated animals was greater (P < 0.001) than slaughtered cattle for different levels of licuri cake. However, the greatest total concentrations of in vitro gases for slaughtered animals were observed when evaluating different levels of crude glycerin (P < 0.001). No differences were observed for diets containing castor bean meal (P >0.05). Thus, the ruminal inoculum obtained from the ruminal contents of slaughtered cattle can replace the use of fistulated animals and is a viable alternative to digestibility analysis. This approach is ethically more correct because it alleviates the suffering of animals by avoiding an invasive procedure.(AU)

O objetivo foi testar a hipótese de que o inóculo ruminal obtido a partir de bovino abatido pode substituir o inóculo do bovino canulado em ensaios que avaliam os alimentos para ruminantes através da produção de gás in vitro e digestibilidade. Foram utilizados cinco novilhos adultos Holstein × Zebu com cânula ruminal para coleta e comparação de líquido ruminal provenientes destes animais in vivo e de animais abatidos. A digestibilidade in vitro da matéria seca (DIVMS) e da fibra em detergente neutro in vitro (DIVFDN) e as concentrações de nitrogênio amoniacal não diferiram entre fontes de inóculo (abatidos × canulados) para todos os subprodutos e níveis testados. A produção total de gás in vitro no inóculo ruminal de animais canulados foi maior (P < 0,001) comparado ao inóculo de animal abatido para os diferentes níveis de torta de licuri. No entanto, as maiores concentrações totais de gás in vitro foram observadas para animais abatidos quando o co-produto utilizado foram níveis de glicerina bruta (P <0,001). Não foram observadas diferenças nas dietas contendo farelo de mamona (P > 0,05). Assim, o inóculo ruminal obtido a partir do conteúdo ruminal de bovino abatido pode substituir o uso de animais fistulados, sendo uma alternativa viável para a realização da análise de digestibilidade. Esta abordagem é eticamente mais correta porque alivia o sofrimento dos animais evitando um procedimento invasivo.(AU)

Assuntos

RESUMO

This study was carried out to evaluate the inclusion of two rice co-products in pig feed during the starter phase in terms of the nutritional value and digestibility of the co-products, and the effect on plasma parameters, growth performance and economic viability. In Experiment I, a digestibility assay was performed using 30 barrows (14.4 ± 2.4 kg body weight), which were distributed in a completely randomised design. The co-products studied were broken rice (BR) and hominy rice 3/4 (HR) to replace the reference diet (300 g/kg). The estimated digestible energy (DE) and metabolisable energy (ME) were 3,476 and 3,360 kcal/kg for BR and 3,487 and 3,362 kcal/kg for HR, respectively. In Experiment II, 108 pigs (15.5 ± 1.0 to 30.1 ± 1.3 kg body weight) were used, distributed in a randomised block design with a factorial analysis scheme (2 × 4), using two types of rice and four inclusion levels (160, 320, 480, and 640 g/kg), with six replicates and two pigs per experimental unit. Additionally, a control diet was used with no inclusion of the co-product. The daily weight gain increased and the feed:gain (F:G) ratio decreased as HR content of feed increased. An effect of both types of rice on plasma glucose concentration was obtained, in which the pigs fed with BR showed the highest values. The cost of diet per kilogram of body weight gain increased as BR levels in the diet increased. It was observed that inclusion levels of 480 and 640 g/kg of BR differed from the control diet, showing the highest costs. HR and BR can be used in pig feed in the starter phase at up to 640 g/kg without impairing performance.(AU)

Este estudo foi realizado para avaliar a inclusão de dois coprodutos do arroz na alimentação de suínos durante a fase inicial sobre o valor nutricional e a digestibilidade dos coprodutos, parâmetros plasmáticos, desempenho zootécnico e a viabilidade econômica. No experimento I, realizou-se um ensaio de digestibilidade, utilizando 30 suínos machos castrados (14.4 ± 2.4 kg de peso corporal), distribuídos em um delineamento inteiramente casualizado. Os coprodutos estudados foram a quirera de arroz (QA) e o arroz canjica 3/4 (AC) em substituição à ração referência (300 g/kg). Os valores estimados de energia digestível (ED) e energia metabolizável (EM) foram de 3.476 e 3.360 kcal/kg para QA e 3.487 e 3.362 kcal/kg para AC, respectivamente. No experimento II, foram utilizados 108 suínos (15.5 ± 1.0 a 30.1 ± 1.3 kg de peso corporal), distribuídos em um delineamento em blocos casualizados, em esquema de análise fatorial (2 x 4), com dois tipos de arroz e quatro níveis de inclusão (160, 320, 480 e 640 g/kg), com seis repetições e dois suínos por unidade experimental. Adicionalmente, foi utilizada uma ração controle, sem inclusão dos coprodutos. O ganho diário de peso aumentou e a conversão alimentar reduziu quando aumentou AC nas rações. Foi obtido efeito dos tipos de arroz sobre a concentração de glicose plasmática, em que os suínos alimentados com QA apresentaram os maiores valores. O custo de ração (CR) por quilograma de ganho de peso corporal aumentou em função dos níveis de QA. Observou-se que os níveis de 480 e 640 g/kg de inclusão de QA diferiram da ração controle, apresentando os maiores custos. O AC e o QA podem ser utilizados, em dietas para suínos na fase inicial, em até o nível de 640 g/kg sem prejudicar o desempenho.(AU)

Assuntos

RESUMO

The aim of this study was to evaluate the effect of increasing levels of cashew nut shell liquid (CNSL) in ruminant diets on in vitro dry matter digestibility (IVDMD), gas production kinetics, ruminal fermentation parameters, ammoniacal nitrogen concentration (NH3-N), and pH of the artificial rumen contents. The experimental design was completely randomized in a factorial 5 x 4 + 1 design, with five concentrate levels (200, 400, 600, 800, and 1,000 g kg-1 DM) and four CNSL levels (0, 0.3, 0.6, and 1.2 g kg-1 DM), as well as a control diet comprising only whole-plant corn silage, totaling 21 treatments. The inclusion of concentrate linearly increased IVDMD, while CNSL levels showed a quadratic effect, with the maximum estimated at 0.5 g kg-1 of CNSL. The total gas production, the disappearance of the substrate, the fraction of slow degradation (fraction VF2), and the respective degradation rate (fraction μ2) linearly increased with increasing levels of concentrate in the diet. Increasing concentrate levels resulted in a linear increase in the concentration of NH3-N and a reduction in the pH of the rumen liquid. Increasing CNSL levels decreased the concentration of NH3-N and increased the ruminal pH. The inclusion of 0.5 g CNSL kg-1 in the ruminant diets improved IVDMD, without altering the kinetic parameters of ruminal fermentation. The addition of CNSL to ruminant diets reduces ammoniacal nitrogen production and can avoid drastic reductions in ruminal pH, favoring better fermentation in the rumen.(AU)

O objetivo deste estudo foi avaliar o efeito do líquido de casca de castanha de caju (LCC) na dieta de ruminantes com níveis crescentes de concentrado sobre a digestibilidade in vitro da matéria seca (DIVMS), cinética de produção de gases e parâmetros ruminais, concentração de nitrogênio amoniacal (N-NH3) e o pH do conteúdo do rúmen artificial. O delineamento experimental foi inteiramente casualizado, em arranjo factorial 5x4+1, com cinco níveis de concentrado (200, 400, 600, 800 e 1000 g kg-1MS) e quatro níveis de LCC (0, 0,3, 0,6 e 1,2 g kg-1 MS), e uma dieta controle, composta apenas por silagem de milho planta inteira, totalizando 21 tratamentos. A inclusão de concentrado aumentou linearmente a DIVMS, enquanto que os níveis LCC mostraram um efeito quadrático, com o máximo estimado de 0,5 g kg-1 de LCC. A produção total de gás, o desaparecimento do substrato, a fracção de degradação lenta (fracção VF2) e a respectiva taxa de degradação (fracção μ2) aumentaram de forma linear com aumento crescente dos níveis de concentrado na dieta. O aumento dos níveis de concentrado resultou em aumento linear na concentração de N-NH3 e na redução no pH do líquido ruminal. O aumento da concentração LCC reduziu os níveis de N-NH3 e aumentou o pH do rúmen. A inclusão de 0,5 g kg-1 de LCC na dieta dos ruminantes melhorou a DIVMS, sem alterar os parâmetros cinéticos de fermentação ruminal. A adição de LCC na dieta de ruminantes reduz a produção de amônia e evitou reduções drásticas no pH ruminal, favorecendo uma melhor fermentação no rúmen.(AU)

Assuntos

RESUMO

The aim of this study was to evaluate the effect of increasing levels of cashew nut shell liquid (CNSL) in ruminant diets on in vitro dry matter digestibility (IVDMD), gas production kinetics, ruminal fermentation parameters, ammoniacal nitrogen concentration (NH3-N), and pH of the artificial rumen contents. The experimental design was completely randomized in a factorial 5 x 4 + 1 design, with five concentrate levels (200, 400, 600, 800, and 1,000 g kg-1 DM) and four CNSL levels (0, 0.3, 0.6, and 1.2 g kg-1 DM), as well as a control diet comprising only whole-plant corn silage, totaling 21 treatments. The inclusion of concentrate linearly increased IVDMD, while CNSL levels showed a quadratic effect, with the maximum estimated at 0.5 g kg-1 of CNSL. The total gas production, the disappearance of the substrate, the fraction of slow degradation (fraction VF2), and the respective degradation rate (fraction μ2) linearly increased with increasing levels of concentrate in the diet. Increasing concentrate levels resulted in a linear increase in the concentration of NH3-N and a reduction in the pH of the rumen liquid. Increasing CNSL levels decreased the concentration of NH3-N and increased the ruminal pH. The inclusion of 0.5 g CNSL kg-1 in the ruminant diets improved IVDMD, without altering the kinetic parameters of ruminal fermentation. The addition of CNSL to ruminant diets reduces ammoniacal nitrogen production and can avoid drastic reductions in ruminal pH, favoring better fermentation in the rumen.

O objetivo deste estudo foi avaliar o efeito do líquido de casca de castanha de caju (LCC) na dieta de ruminantes com níveis crescentes de concentrado sobre a digestibilidade in vitro da matéria seca (DIVMS), cinética de produção de gases e parâmetros ruminais, concentração de nitrogênio amoniacal (N-NH3) e o pH do conteúdo do rúmen artificial. O delineamento experimental foi inteiramente casualizado, em arranjo factorial 5x4+1, com cinco níveis de concentrado (200, 400, 600, 800 e 1000 g kg-1MS) e quatro níveis de LCC (0, 0,3, 0,6 e 1,2 g kg-1 MS), e uma dieta controle, composta apenas por silagem de milho planta inteira, totalizando 21 tratamentos. A inclusão de concentrado aumentou linearmente a DIVMS, enquanto que os níveis LCC mostraram um efeito quadrático, com o máximo estimado de 0,5 g kg-1 de LCC. A produção total de gás, o desaparecimento do substrato, a fracção de degradação lenta (fracção VF2) e a respectiva taxa de degradação (fracção μ2) aumentaram de forma linear com aumento crescente dos níveis de concentrado na dieta. O aumento dos níveis de concentrado resultou em aumento linear na concentração de N-NH3 e na redução no pH do líquido ruminal. O aumento da concentração LCC reduziu os níveis de N-NH3 e aumentou o pH do rúmen. A inclusão de 0,5 g kg-1 de LCC na dieta dos ruminantes melhorou a DIVMS, sem alterar os parâmetros cinéticos de fermentação ruminal. A adição de LCC na dieta de ruminantes reduz a produção de amônia e evitou reduções drásticas no pH ruminal, favorecendo uma melhor fermentação no rúmen.

Assuntos

RESUMO

This study was carried out to evaluate the inclusion of two rice co-products in pig feed during the starter phase in terms of the nutritional value and digestibility of the co-products, and the effect on plasma parameters, growth performance and economic viability. In Experiment I, a digestibility assay was performed using 30 barrows (14.4 ± 2.4 kg body weight), which were distributed in a completely randomised design. The co-products studied were broken rice (BR) and hominy rice 3/4 (HR) to replace the reference diet (300 g/kg). The estimated digestible energy (DE) and metabolisable energy (ME) were 3,476 and 3,360 kcal/kg for BR and 3,487 and 3,362 kcal/kg for HR, respectively. In Experiment II, 108 pigs (15.5 ± 1.0 to 30.1 ± 1.3 kg body weight) were used, distributed in a randomised block design with a factorial analysis scheme (2 × 4), using two types of rice and four inclusion levels (160, 320, 480, and 640 g/kg), with six replicates and two pigs per experimental unit. Additionally, a control diet was used with no inclusion of the co-product. The daily weight gain increased and the feed:gain (F:G) ratio decreased as HR content of feed increased. An effect of both types of rice on plasma glucose concentration was obtained, in which the pigs fed with BR showed the highest values. The cost of diet per kilogram of body weight gain increased as BR levels in the diet increased. It was observed that inclusion levels of 480 and 640 g/kg of BR differed from the control diet, showing the highest costs. HR and BR can be used in pig feed in the starter phase at up to 640 g/kg without impairing performance.

Este estudo foi realizado para avaliar a inclusão de dois coprodutos do arroz na alimentação de suínos durante a fase inicial sobre o valor nutricional e a digestibilidade dos coprodutos, parâmetros plasmáticos, desempenho zootécnico e a viabilidade econômica. No experimento I, realizou-se um ensaio de digestibilidade, utilizando 30 suínos machos castrados (14.4 ± 2.4 kg de peso corporal), distribuídos em um delineamento inteiramente casualizado. Os coprodutos estudados foram a quirera de arroz (QA) e o arroz canjica 3/4 (AC) em substituição à ração referência (300 g/kg). Os valores estimados de energia digestível (ED) e energia metabolizável (EM) foram de 3.476 e 3.360 kcal/kg para QA e 3.487 e 3.362 kcal/kg para AC, respectivamente. No experimento II, foram utilizados 108 suínos (15.5 ± 1.0 a 30.1 ± 1.3 kg de peso corporal), distribuídos em um delineamento em blocos casualizados, em esquema de análise fatorial (2 x 4), com dois tipos de arroz e quatro níveis de inclusão (160, 320, 480 e 640 g/kg), com seis repetições e dois suínos por unidade experimental. Adicionalmente, foi utilizada uma ração controle, sem inclusão dos coprodutos. O ganho diário de peso aumentou e a conversão alimentar reduziu quando aumentou AC nas rações. Foi obtido efeito dos tipos de arroz sobre a concentração de glicose plasmática, em que os suínos alimentados com QA apresentaram os maiores valores. O custo de ração (CR) por quilograma de ganho de peso corporal aumentou em função dos níveis de QA. Observou-se que os níveis de 480 e 640 g/kg de inclusão de QA diferiram da ração controle, apresentando os maiores custos. O AC e o QA podem ser utilizados, em dietas para suínos na fase inicial, em até o nível de 640 g/kg sem prejudicar o desempenho.

Assuntos

RESUMO

The objective was to test the hypothesis that ruminal inoculum obtained from slaughtered cattle can replace inoculum from cannulated cattle in trials evaluating animal feedstuffs through in vitro gas production and digestibility. Five adult Holstein × Zebu steers with ruminal cannula were used to collect and compare rumen liquid from in vivo and slaughtered animals. In vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD) and ammoniacal nitrogen concentrations did not differ between inoculum sources (slaughtered × cannulated) for all byproducts and levels tested. Total in vitro gas production in the ruminal inoculum of cannulated animals was greater (P 0.05). Thus, the ruminal inoculum obtained from the ruminal contents of slaughtered cattle can replace the use of fistulated animals and is a viable alternative to digestibility analysis. This approach is ethically more correct because it alleviates the suffering of animals by avoiding an invasive procedure.

O objetivo foi testar a hipótese de que o inóculo ruminal obtido a partir de bovino abatido pode substituir o inóculo do bovino canulado em ensaios que avaliam os alimentos para ruminantes através da produção de gás in vitro e digestibilidade. Foram utilizados cinco novilhos adultos Holstein × Zebu com cânula ruminal para coleta e comparação de líquido ruminal provenientes destes animais in vivo e de animais abatidos. A digestibilidade in vitro da matéria seca (DIVMS) e da fibra em detergente neutro in vitro (DIVFDN) e as concentrações de nitrogênio amoniacal não diferiram entre fontes de inóculo (abatidos × canulados) para todos os subprodutos e níveis testados. A produção total de gás in vitro no inóculo ruminal de animais canulados foi maior (P 0,05). Assim, o inóculo ruminal obtido a partir do conteúdo ruminal de bovino abatido pode substituir o uso de animais fistulados, sendo uma alternativa viável para a realização da análise de digestibilidade. Esta abordagem é eticamente mais correta porque alivia o sofrimento dos animais evitando um procedimento invasivo.

Assuntos