RESUMO

The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by 6 nM TPA. The TPA analog 4 alpha PDD, an ineffective activator of PKC, did not affect spore germination. 3 mM dbcAMP, on the other hand, reversed the inhibition of germination caused by TPA. TPA-stimulated protein kinase activity was detected in spores. The possible relationship between PKC and the increased levels of cAMP that accompany the induction of spore germination is discussed.

Assuntos

Mucorales/fisiologia , Phycomyces/fisiologia , Proteína Quinase C/metabolismo , Acetato de Tetradecanoilforbol/farmacologia , Bucladesina/farmacologia , Diglicerídeos/farmacologia , Ativação Enzimática/efeitos dos fármacos , Ésteres de Forbol/farmacologia , Phycomyces/efeitos dos fármacos , Esporos Fúngicos/efeitos dos fármacos , Esporos Fúngicos/fisiologia