RESUMO
Disturbances in lipid metabolism during copper deficiency in rats are well recognized. Copper deficiency is associated with the spontaneous retention of hepatic iron. Previous studies have reported that hypercholesterolemia and hypertriglyceridemia are associated with elevated hepatic iron concentrations in copper deficient rats. There was a direct relationship between the magnitude of blood lipids and the concentration of hepatic iron. Based on these data, it has been hypothesized that iron was responsible for the development of lipemia of copper deficiency. In this study was determined the effect of increasing doses of Cu(10, 20 and 50 ppm) in the diet, on the serum total lipids, total cholesterol, triglycerides (triacylglicerols), phospholipids, non-esterified fatty acids (NEFA) and liver iron and zinc concentrations in normal rats. The results were compared with normal rats that received a balanced diet containing 0.6 and 6 ppm of Cu, respectively. The results show that Cu-supplement diminished the cholesterol and triglyceride serum levels, increased the level of phospholipids, NEFA and concomitantly decreased the hepatic concentrations of Fe and Zn. There was a statistically significant (p < 0.05) simple correlation between triglycerides and liver Fe (r = 0.917; R2 = 64.03%), cholesterol and liver Zn (r = 0.872; R2 = 76.07%), cholesterol and liver Fe (r = 0.995; R2 = 99.10%), liver Fe and liver Cu (r = -0.612), liver Fe and liver Zn (r = 0.837), liver Cu and liver Zn (r = -0.612), and serum triglycerides and liver Zn (r = 0.967). The mechanism(s) by which Fe and Zn determine these changes is not known; none of the enzymes that act in cholesterol and triglyceride metabolism and biosynthesis require Fe and/or Zn. The increase of NEFA is due to changes in the process of lipolysis and re-esterification of the fatty acids in blood. However, additional studies are needed for the precise mechanisms of this interrelationships to be clarified.
Assuntos
Cobre/administração & dosagem , Suplementos Nutricionais , Ferro/análise , Lipídeos/sangue , Fígado/química , Zinco/análise , Animais , Estudos de Casos e Controles , Colesterol/sangue , Cobre/deficiência , Masculino , Fosfolipídeos/sangue , Ratos , Ratos Wistar , Triglicerídeos/sangueRESUMO
En el presente trabajo se cuantificó el contenido sérico de las mucoproteínas y de las proteínas combinadas con hexosas (PBH) o con ácido siálico (PAS) en 440 escolares portadores de diversas patologías (GRUPO DE CASOS) y se comparó con los hallazgos obtenidos en 160 escolares sanos con características similares a los casos (GRUPO TESTIGO). Los valores de mucoproteínas, PBH y PAS en los escolares sanos fueron 15ñ9, 112ñ20 y 66ñ12 mg/dL, respectivamente, los cuales se utilizaron para comparar con los casos. En los escolares enfermos no tratados los niveles de estas seroglicoproteínas difieren significativamente (p<0.05), dependiendo de la patología en estudio, al comparar con los valores normales y de los escolares tratados. En las piodermitis y en las parasitosis se encuentran valores de PBH inferiores al rango normal, igual fenómeno se detectó con las mucoproteínas en las hepatitis virales e infecciones agudas. Cuando los tratamientos son adecuados, los valores alterados de las glicoproteínas rápidamente retornan a la normalidad. Se concluye que la determinación de estas seroglicoproteínas puede ser un auxiliar útil para evaluar la respuesta de los pacientes al tratamiento (s) y en casos de diagnóstico difícil, tal como sucede en los escolares ictéricos
Assuntos
Humanos , Masculino , Feminino , Hexoses , Mucoproteínas , Ácido N-Acetilneuramínico , VenezuelaRESUMO
The relation of excessive doses of vitamin A with various kidney pathologies is well known however, information concerning the relation of kidney enzyme activity with acute hypervitaminosis A is rather scarce. In this study we describe the kidney enzymatic alterations observed in rats that received daily intramuscular injections of 10,000, 30,000, 50,000 and 100,000 IU of vitamin A palmitate (VA) during seven days (TREATED GROUPS). A comparison is made with the enzyme activity in healthy rats pair-fed and treated with sodium palmitate by intramuscular injection (CONTROL GROUP). The treated rats showed a proportional increase (p < 0.05) in activity of acid maltase, transminases or aminotransferases (GOT and GPT), alkaline phosphatase (ALP) and acid protease with all doses of VA administered. Amylase, lipase and arginase tend to decrease (p < 0.05) in activity only with doses of 50,000 and 100,000 I.U. of VA. Several factors are responsible for these findings, such as kidney necrosis due to release of lysosomal acid hydrolases produced by hypervitaminosis A.
Assuntos
Alanina Transaminase/metabolismo , Aspartato Aminotransferases/metabolismo , Hidrolases/metabolismo , Hipervitaminose A/metabolismo , Rim/enzimologia , Fosfatase Alcalina/metabolismo , Animais , Arginase/metabolismo , Glucana 1,4-alfa-Glucosidase/metabolismo , Lipase/metabolismo , Masculino , Ratos , Ratos WistarRESUMO
The relation of excessive doses of vitamin A with various kidney pathologies is well known however, information concerning the relation of kidney enzyme activity with acute hypervitaminosis A is rather scarce. In this study we describe the kidney enzymatic alterations observed in rats that received daily intramuscular injectiosn of 10.000, 30.000, 50.000 and 100.000IU of vitamin A palmitate (VA) during seven days (TREATED GROUPS). A comparison is made with the enzyme activity in healthy rats pairfed and treated with sodium palmitte by intramuscular injection (CONTROL GROUP). The treated rats showed a proportional increase (<0.05) in activity of acid maltase, transaminases or aminotransferase (GOT and GPT), alkaline phosphatase (ALP) and acid protease with all doses of VA administered, Amylase, lipase and arginase tend to decrease (p<0.05) in activity only with doses of 50.000 and 100.000 I.U. of VA. Several factors are responsible for these findings, such as kidney necrosis due to release of lysosomal acid hydrolases produced by hypervitaminosis A.
Assuntos
Animais , Masculino , Ratos , Alanina Transaminase/metabolismo , Aspartato Aminotransferases/metabolismo , Hidrolases/metabolismo , Hipervitaminose A/metabolismo , Rim/enzimologia , Fosfatase Alcalina/metabolismo , Arginase/metabolismo , Glucana 1,4-alfa-Glucosidase/metabolismo , Lipase/metabolismo , Distribuição Aleatória , Ratos WistarRESUMO
En el presente estudio se describe el efecto de dosis elevadas de vitamina K3 (VK: 10 a 50 mg/kg/día) inyectadas intramuscularmente, durante un lapso de 7 días, sobre el contenido sérico y hepático de Ca,Mg,Pi y Cl en ratas blancas macho y se comparan los resultados con lo que sucede en ratas tratadas con dosis iguales de bisulfito de sodio. Los resultados obtenidos demuestran que la vitamina K a dosis elevadas produce una marcada alteración en el contenido sérico y hepático de Ca,Mg y de Pi sin modificar el contenido de Cl. En consecuencia, la hipervitamina K, al igual que lo que sucede con las vitaminas A y D, puede alterar significativamente el metabolismo mineral con graves consecuencias para las diversas actividades enzimáticas corporales
Assuntos
Animais , Ratos , Sangue/metabolismo , Cálcio/administração & dosagem , Fígado/metabolismo , Magnésio/administração & dosagem , Vitamina K/administração & dosagem , Farmácia , VenezuelaRESUMO
El interés sobre las glicoproteínas séricas, incluyendo las mucoproteínas, se reflejan en la gran cantidad de publicaciones que existen sobre el tema. La mayoría de ellas se refiere a personas adultas no encontrándose ningún estudio completo sobre estos compuestos durante la niñez y la adolescencia. En la presente investigación se cuantificó el contenido sérico de las mucoproteínas en 320 escolares en buen estado de salud:160 niños y 160 niñas, entre 7 y 14 años de edad, procedentes de la ciudad de Mérida(Venezuela). Las mucoproteínas alcanzaron un promedio de 15ñ4mg/dl(rango=88-18), no demostrándose ninguna variación significativa en este valor en relación con la edad o con el sexo de los escolares sometidos al estudio