RESUMO
Here we report that leukemia cell lines and primary CD34+ leukemic blasts exposed to platelet rich plasma (PRP) or platelet lysates (PL) display increased resistance to apoptosis induced by mitochondria-targeted agents ABT-737 and CDDO-Me. Intriguingly, leukemia cells exposed to platelet components demonstrate a reduction in mitochondrial membrane potential (ΔΨM) and a transient increase in oxygen consumption, suggestive of mitochondrial uncoupling. Accompanying the ranolazine-sensitive increase in oxygen consumption, a reduction in triglyceride content was also observed in leukemia cells cultured with platelet components indicating that lipolysis and fatty acid oxidation may support the molecular reduction of oxygen in these cells. Mechanistically, platelet components antagonized Bax oligomerization in accordance with previous observations supporting an antiapoptotic role for fatty acid oxidation in leukemia cells. Lastly, substantiating the notion that mitochondrial uncoupling reduces oxidative stress, platelet components induced a marked decrease in basal and rotenone-induced superoxide levels in leukemia cells. Taken together, the decrease in ΔΨM, the transient increase in ranolazine-sensitive oxygen consumption, the reduction in triglyceride levels, and the reduced generation of superoxide, all accompanying the increased resistance to mitochondrial apoptosis, substantiate the hypothesis that platelets may contribute to the chemoprotective sanctuary of the bone marrow microenvironment via promotion of mitochondrial uncoupling.
RESUMO
En este artículo se describe un caso clínico poco frecuente de osteosarcoma generalizado acompañado de megaesófago secundario adquirido, ocasionados por un granuloma parasitario torácico de Spirocerca lupi, en un canino bóxer adulto. Se presentan los hallazgos radiológicos y anatomo-patológicos y se discuten con la literatura revisada.
Assuntos
Cães , Acalasia Esofágica/veterinária , Doenças do Cão , Osteossarcoma , ThelazioideaRESUMO
En este artículo se describe un caso clínico de subluxación parcial del carpo derecho (antebraquiocarpiana e intercarpiana) de un canino de 14 meses de edad. Se discute el diagnóstico, tratamiento médico-quirúrgico, rehabilitación física y se comparan los resultados obtenidos con la literatura.
Assuntos
Cães , Artrodese , Carpo Animal , Cães , Fraturas Ósseas/veterináriaRESUMO
En este articulo se reporta un caso de megaesófago idiopático congénito en un cachorro caniche de dos meses de edad. Se muestran detalladamente las alteraciones anatómicas observadas en el estudio radiológico con medio de contraste positivo (sulfato de Bario). Se hace una revisión de literatura sobre esta anomalía y se comparan las alteraciones patológicas del paciente de este estudio con la literatura internacional.