RESUMO
This experimental light microscopy study investigated the formation of a hybrid layer and resin tags on sound dentin, after utilization of conventional and self-etching adhesive systems. After restorative procedures, the specimens were decalcified in a formic acid and sodium citrate solution, embedded in paraffin, sectioned at 6-microm thickness and stained by the Brown & Brenn method for analysis and measurement by light microscopy (AXIOPHOT) (400x). The results were statistically analyzed by analysis of variance, at a significance level of 5%. Based on the results, it could be concluded that the conventional adhesive allowed the formation of a thicker hybrid layer than the self-etching adhesive, with similar penetration into the dentinal tubules (resin tags).
Assuntos
Cimentos Dentários , Corrosão Dentária , Dentina , Adulto , Humanos , Técnicas In Vitro , Adulto JovemRESUMO
This experimental light microscopy study investigated the formation of a hybrid layer and resin tags on sound dentin, after utilization of conventional and self-etching adhesive systems. Afterrestorative procedures, the specimens were decalcified in a formic acid and sodium citrate solution, embedded in paraffin, sectioned at 6-ým thickness and stained by the Brown & Brenn method for analysis and measurement by light microscopy (AXIOPHOT) (400x). The results were statistically analyzed by analysis of variance, at a significance level of 5%. Based on the results, it could be concluded that the conventional adhesive allowed the formation of a thicker hybrid layer than the self-etching adhesive, with similar penetration into the dentinal tubules (resin tags).(AU)
Analisamos nesta pesquisa laboratorial/microscópica, o poder deformaþÒo da camada híbrida de adesÒo e dos prolongamentos resinosos (tags) em tecido dentinário hígido, empregando, para tanto, nesse substrato dental, um sistema adesivo convencional e outro autocondicionante. Após a realizaþÒo dos procedimentosrestauradores; os espécimes foram descalcificados em soluþÒo de ácido fórmico/citrato de sódio, incluídos em parafina, cortados a uma espessura de 6 micrometros, corados pelo método Brown & Brenn, para posteriormente, serem analisados e mensurados emmicroscopia óptica comum (AXIOPHOT) (400X). Após a coleta dos resultados, estes foram submetidos O análise estatística ANOVA, ao nível de 5%. De acordo com os resultados obtidospudemos concluir que o adesivo convencional possibilitou a formaþÒode uma camada híbrida de adesÒo mais espessa do que o adesivo autocondicionante e um mesmo nível penetraþÒo no interior do túbulo dentinário (Tags).(AU)
Assuntos
Resinas Compostas , Adesivos Dentinários/química , Condicionamento Ácido do Dente/métodos , Dentina/efeitos dos fármacos , Análise de Variância , Microscopia/métodosRESUMO
This experimental light microscopy study investigated the formation of a hybrid layer and resin tags on sound dentin, after utilization of conventional and self-etching adhesive systems. Afterrestorative procedures, the specimens were decalcified in a formic acid and sodium citrate solution, embedded in paraffin, sectioned at 6-ìm thickness and stained by the Brown & Brenn method for analysis and measurement by light microscopy (AXIOPHOT) (400x). The results were statistically analyzed by analysis of variance, at a significance level of 5%. Based on the results, it could be concluded that the conventional adhesive allowed the formation of a thicker hybrid layer than the self-etching adhesive, with similar penetration into the dentinal tubules (resin tags).
Analisamos nesta pesquisa laboratorial/microscópica, o poder deformação da camada híbrida de adesão e dos prolongamentos resinosos (tags) em tecido dentinário hígido, empregando, para tanto, nesse substrato dental, um sistema adesivo convencional e outro autocondicionante. Após a realização dos procedimentosrestauradores; os espécimes foram descalcificados em solução de ácido fórmico/citrato de sódio, incluídos em parafina, cortados a uma espessura de 6 micrometros, corados pelo método Brown & Brenn, para posteriormente, serem analisados e mensurados emmicroscopia óptica comum (AXIOPHOT) (400X). Após a coleta dos resultados, estes foram submetidos à análise estatística ANOVA, ao nível de 5%. De acordo com os resultados obtidospudemos concluir que o adesivo convencional possibilitou a formaçãode uma camada híbrida de adesão mais espessa do que o adesivo autocondicionante e um mesmo nível penetração no interior do túbulo dentinário (Tags).
Assuntos
Adesivos Dentinários/química , Resinas Compostas , Dentina , Condicionamento Ácido do Dente/métodos , Análise de Variância , Microscopia/métodosRESUMO
Twenty-four monoclonal antibodies (mAbs) against group B Neisseria meningitidis surface antigens were analyzed by immunoenzymatic assays and by a bactericidal test. Two mAbs were specific to polysaccharide B and one to lipopolysaccharide. The others were specific to polysaccharide B and one to lipopolysaccharide. The others were directed against outer membrane proteins ranging in molecular mass from 25 to 200 kDa. The outer membrane protein epitopes recognized by the mAbs were not conformational and were located on the outer surface of the microorganism. Linear epitopes on the class 5 protein, exposed on the surface of the membrane, were able to induce bactericidal antibodies to the homologous strain. The susceptibility of Neisseria meningitidis to these antibodies was unchanged when this organism was cultivated under conditions of iron depletion. These results demonstrate that peptides derived from class 5 proteins are potentially important in synthetic peptide or in recombinant protein vaccines containing linear bactericidal epitopes.