RESUMO
We report the first case of systemic lupus erythematosus with hyperviscosity syndrome as the presenting manifestation. A polyclonal increase in all immunoglobulins was observed. Ultracentrifuge studies showed 17-19S intermediate immune complexes (IC) composed primarily of autoreactive IgG demonstrated at acid pH. This was confirmed by chromatographic fractionation of sera, since the intermediate IC eluted as did purified heat aggregated IgG. Small amounts of IgM and IgA in the intermediate IC were also demonstrated by more sensitive techniques, i.e., nephelometry, polyacrylamide gel electrophoresis, and electrophoretic blotting. The decrease of serum viscosity was associated with the decrease of circulating IC after plasmapheresis or combined treatment of prednisone and cyclophosphamide. Serum viscosity bore no relationship to the amount of dissociated immunoglobulins.
Assuntos
Viscosidade Sanguínea , Lúpus Eritematoso Sistêmico/sangue , Adulto , Complexo Antígeno-Anticorpo/análise , Cromatografia em Gel , Eletroforese em Gel de Poliacrilamida , Humanos , Imunoglobulinas/análise , Focalização Isoelétrica , MasculinoRESUMO
Complement depletion in the hamster by cobra venom factor (CoF) gives a higher frequency and severity of lesions after intrahepatic inoculation of Entamoeba histolytica. The CoF-treatment does not alter the humoral immune response to amebae, indicating that its effect on the amebic liver abscess is not due to suppression of the immune response but rather to its effect on complement levels at the time of the protozoal infection.
Assuntos
Proteínas Inativadoras do Complemento/farmacologia , Proteínas do Sistema Complemento/análise , Venenos Elapídicos/farmacologia , Abscesso Hepático Amebiano/imunologia , Animais , Cricetinae , Abscesso Hepático Amebiano/tratamento farmacológico , MesocricetusRESUMO
The activation of the alternative pathway of complement by axenic cultures of E. histolytica is reported. The activation is carried out in different proportions by several axenic strains, i.e., HK9:NIH, HM1:IMSS, HM2:IMSS and HM3:IMSS; the end result consists of a destruction of the parasite as well as consumption of the serum complement. The participation of the alternative pathway was studied in the presence of EGTA and Mg++, zymosan, heat-inactivation at 50 degree C for 20 min and immunoelectrophoretic studied with anti-factor B.
Assuntos
Ativação do Complemento , Complemento C3 , Via Alternativa do Complemento , Entamoeba histolytica/imunologia , Animais , Entamoeba histolytica/patogenicidade , VirulênciaRESUMO
A passive hemagglutination method useful for the determination of antibodies against Entamoeba histolytica is described. The E. histolytica antigen is attached to group "O" human red blood cells by means of CrCl3. The specificity and sensitivity of the CrCl3 method was compared with other hemagglutination methods, i.e., tannic acid method and a commercial preparation, as well as counterimmunoelectrophoresis. The CrCl3 method showed a reactivity similar to the tannic acid hemagglutination technique and greater specificity than the commercial preparation. The assay reported here showed a good correlation with the counterimmunoelectrophoresis method. The sensitization of erythrocytes with the amebic antigen by means of CrCl3 offers an easy, rapid and specific technique which can be used as a routine test for the detection of antibodies against E. histolytica.