RESUMO
Chikungunya virus (CHIKV) is an RNA virus transmitted by Aedes mosquitoes. The clinical manifestations include fever, arthralgia, rash, and other atypical clinical findings including ocular lesions. We report the case of a 57-year-old man with meningoencephalitis and anterior uveitis due to CHIKV. The patient had developed bilateral anterior uveitis with iris atrophy and a cotton wool spot on the left eye, and his serum, urine, saliva, and cerebrospinal fluid were positive for CHIKV by RT-PCR. The spectrum of the ophthalmologic manifestations and its pathophysiology in cases of CHIKV infections needs to be better understood. Additional studies examining the ocular lesions caused by CHIKV could improve the therapeutic goals of reducing the morbidity and sequels.
Assuntos
Febre de Chikungunya/complicações , Encefalite Viral/etiologia , Infecções Oculares Virais/etiologia , Uveíte Anterior/etiologia , Doença Aguda , Aedes/virologia , Animais , Humor Aquoso/virologia , Febre de Chikungunya/transmissão , Febre de Chikungunya/virologia , Vírus Chikungunya/genética , Encefalite Viral/diagnóstico , Encefalite Viral/virologia , Infecções Oculares Virais/diagnóstico , Infecções Oculares Virais/virologia , Humanos , Imageamento por Ressonância Magnética , Masculino , Microscopia Acústica , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , RNA Viral/análise , Tomografia de Coerência Óptica , Uveíte Anterior/diagnóstico , Uveíte Anterior/virologiaRESUMO
The presence of antibodies against BHV-1 was evaluated in young bovines from fifteen different counties distributed along North, South and Central regions of the state of Bahia. Bovines with age ranged from 1 to 4 years old, non-vaccinated against BHV-1 and without diagnostic of BHV-1 infection were chosen for blood collection. A total of 558 serum samples were collected equivalent to 10% of the population in each farm. The sera were submitted to ELISA (HerdCheck-IDDEX) and Microserum neutralization (MSN) tests. The MSN was performed in monolayers of MDBK cells in microplates using Los Angeles BHV-1 strain. The results of serum samples by ELISA showed that 56% (314/558) were positive and 44% (244/558) were negative. The sera assayed by MSN showed that 48% (269/558) were negative and 52% (289/558) were positive. These positive sera demonstrated that 54% had serumneutralization titer between 4-16, 35% between 32-64 and 11% >; or = 64. Our results suggest that the virus is circulating actively in herds. Furthermore, the high diffusion of the BHV-1 in young bovines could represent a constant source of viral infection in the herd.
A presença de anticorpos contra Herpesvírus Bovino tipo 1 (HBV-1) foi avaliada em bovinos jovens provenientes de quinze municípios diferentes, distribuídos nas regiões Norte, Sul e Central do estado da Bahia. Foram coletadas amostras de sangue de bovinos de 1 a 4 anos de idade, não vacinados contra HBV-1 e sem prévio diagnóstico de infecção pelo vírus HBV-1. O total de amostras correspondeu a 558 soros, equivalente a 10% da população bovina de cada fazenda. Os 558 soros foram submetidos à técnica de Microssoroneutralização (MSN) em microplacas, utilizando-se monocamadas de células MDBK e a cepa de HBV-1 Los Angeles, e ao teste de ELISA comercialmente adquirido: HerdCheck-IDDEX. Os resultados da amostragem feito pelo teste de ELISA mostraram que 56% (314/558) dos soros foram positivos e 44% (244/558), negativos. A técnica de MSN mostrou que 48% (269/558) dos soros foram negativos e 52% (289/558) foram positivos. Destes soros positivos, 54% tiveram títulos soroneutralizantes entre 4-16, 35% entre 32-64, e 11% >; ou = 64. Os resultados sugerem que o HBV-1 esteja circulando ativamente na população bovina jovem. Por outra parte, a alta difusão do vírus em animais jovens representaria uma fonte contínua de infecção viral para o rebanho.