RESUMO
The genus Haemagogus (Diptera: Culicidae) includes species that are important vectors of pathogens such as the yellow fever virus. The accurate identification of these species is essential for the control of zoonoses. Females of Hg. capricornii and Hg. janthinomys are morphologically indistinguishable, which makes the use of alternative identification techniques desirable. This study aimed to obtain sequences of the mitochondrial cytochrome c oxidase I (COI) gene, in the region widely used for DNA barcoding, of Haemagogus specimens from the state of São Paulo, Brazil, to evaluate the effectiveness of these sequences in the molecular identification of the species. A total of 37 female and 2 male mosquitoes were collected in various locations in the state of São Paulo, using methods such as hand-nets, Shannon traps, CDC light traps with CO2 bait and Nasci aspirators. The sequences of a 710 bp fragment of the COI gene were amplified by PCR and sequenced. A phylogenetic tree reconstruction was conducted using the Bayesian approach implemented in MrBayes v3.2.2, providing support values for taxa where genetic clusters may indicate the presence of new or cryptic species. We obtained 39 COI sequences representing three species: Haemagogus capricornii, Haemagogus leucocelaenus, and Haemagogus janthinomys. Bayesian analysis of the sequences produced clades that corroborate the morphological identification of the species. The separation of Hg. capricornii and Hg. janthinomys received 100 % statistical support and the Hg. capricornii was very well supported (91 %). The two sequences from male specimens, morphologically identified as Hg. capricornii, were grouped in the same clade, a sister clade of Hg. janthinomys. It is important to highlight that the Hg. janthinomys were positioned in several subclades, showing a polymorphism of this species within the state, a situation not observed for Hg. capricornii. For the first time, sequences of the mtCOI gene from Hg. capricornii were obtained and related to morphologically identified specimens. COI sequences proved effective in the molecular identification of Haemagogus species. This study contributes to the expansion of the GenBank database, providing the first sequences of Hg. capricornii and new sequences for Hg. janthinomys and Hg. leucocelaenus.
Assuntos
Culicidae , Código de Barras de DNA Taxonômico , Complexo IV da Cadeia de Transporte de Elétrons , Filogenia , Animais , Complexo IV da Cadeia de Transporte de Elétrons/genética , Código de Barras de DNA Taxonômico/métodos , Culicidae/classificação , Culicidae/genética , Masculino , Feminino , Brasil , Análise de Sequência de DNARESUMO
Metagenomic studies of mosquito viromes demonstrated a more diverse composition than just an exclusive composition of pathogenic arboviruses transmitted to humans. In our study, the virome of 866 female mosquitoes collected throughout 2020 at the São Paulo Zoo, located in the city of São Paulo/SP-Brazil, was obtained. Specifically, in this paper, we describe a new virus found by viral RNA extraction and next-generation MiSeq sequencing of a group of 23 specimens of Anopheles (Nys.) strodei. The complete genome with a length of 9709 nucleotides was characterized by a positive orientation and a single strand, with a single large ORF, which encodes a polyprotein of 2987 amino acids. The phylogenetic analysis showed an association with the viral family Iflaviridae and the Riboviria realm. We carried out comparisons with translated sequences of the capsid regions of other iflavirus, and the identities in relation to our sequence were below the minimum limit of 90%, indicating that possibly it is a new species of iflavirus. Our findings contribute to expanding knowledge of virome composition among mosquito species in Brazil and globally. Moreover, we provide a viral genome reference specific to this geographic region and Culicidae family of mosquitoes. This resource facilitates future in silico recognition and assembly of viral genomes within metagenomic datasets.
RESUMO
Information systems are essential instruments in managing resources, in the evaluation of the epidemiological situation, and for decision-making at all hierarchical levels. Technological advances have allowed the development of systems that meet these premises. Therefore, it is recommended to consider the optimization of data entry and its immediate georeferencing in order to obtain information in real time. To meet this objective, we describe the application introduction process for the implementation of the digital collection of primary data and its integration with the database through synchronization with the SisaWeb platform (Information System for surveillance and control of Aedes aegypti), developed to meet the needs of the Arbovirus Surveillance and Control Program in the state of São Paulo, Brazil. For this purpose, the application-SisaMob-was conceived in the Android Studio development environment, Google®, following the same guidelines as the traditional collection method. Tablets equipped with the Android® operating system were used. To evaluate the implementation of the application, a semi-structured test was applied. The results highlighted that 774.9% (27) of the interviewees evaluated its use positively and, replacing the standard bulletin, 61.1% (22) of the users considered it regular to excellent. The automatic collection of geographic coordinates represented the greatest innovation in the use of the portable device, with reductions in errors and in the time taken to complete the report in the field. The integration to SisaWeb allowed obtaining information in real-time, being easily presented in tabular and graphic modes and spatially arranged through maps, making it possible to monitor the work at a distance, and allowing preliminary analyses during the data collection process. For the future, we must improve the mechanisms for assessing the effectiveness of information, increase the potential of the tool to produce more accurate analyses, which can direct actions more efficiently.
RESUMO
The identification of mosquito species is necessary for determining the entomological components of disease transmission. However, identification can be difficult in species that are morphologically similar. The cytochrome c oxidase subunit I (COI) DNA barcode region is considered a valuable and reliable diagnostic tool for mosquito species recognition, including those that belong to species complexes. Mansonia mosquitoes are found in forests near swampy areas. They are nocturnal and are highly attracted to light. Hematophagous adult females exhibit aggressive biting behavior and can become infected with and transmit pathogens during their feeding, including some epizootic viruses and avian malaria. In Brazil, twelve Mansonia species have been reported. In a recent study from the São Paulo Zoo in Brazil, three morphologically distinct species were collected and identified, namely: Mansonia (Mansonia) indubitans, Ma. (Man.) pseudotitillans and Ma. (Man.) titillans. However, confirmation of these species by molecular identification was unsuccessful due to a lack of COI sequences in the GenBank database. Thus, this research aimed to describe the COI DNA barcode sequences of some morphologically characterized Mansonia (Man.) species from Brazil and to determine their utility in delimiting species collected from the Atlantic Forest and Brazilian Savanna. Accordingly, we provide tools for the genetic identification of species that play a significant role in pathogen transmission in wildlife and potentially humans. We show that the delimitation of Mansonia species via five different approaches based on COI DNA sequences (BI, NJ, ASAP, bPTP and GMYC) yield basically the same groups identified by traditional taxonomy, and we provide the identification of specimens that were previously identified only up to the subgenus level. We also provide COI sequences from two Mansonia species that were not previously available in sequence databases, Ma. wilsoni and Ma. pseudotitillans, and thus contribute to the ongoing global effort to standardize DNA barcoding as a molecular means of species identification.
RESUMO
The hand-net is the standard method for capturing mosquitoes with sylvatic diurnal activity in disease outbreaks in Brazil. However, occupational risks and biases related to the collectors' abilities and attractiveness are important limitations. In this study, we compared hand-nets with automatic traps (CDC) associated to CO2 and BG-Lure® in the Vassununga State Park, a Brazilian Savanna protection area. The collections carried out over 27 days on the ground and the forest canopy. A total of 1555 mosquitoes were obtained in 20 taxa. The diversity index ranged between 1.12 and 1.79 and the dominance index from 0.22 to 0.40. The dominant species on the ground was Aedes scapularis (46.0%), and in the canopy, Hg. janthinomys/capricornii (31.9%). Haemagogus leucocelaenus was rare (n = 2). The hand-net resulted in the greatest diversity and abundance of species in both strata, followed by the traps associated with CO2. A low degree of similarity was observed between the hand-net on the ground compared to the other capture methods. The use of BG-Lure® alone resulted in a low number of specimens. In conclusion, the hand-net is still the method of choice for collecting arbovirus vectors in the diurnal period, especially yellow fever vectors.
RESUMO
Hand-held insect nets are the standard method for capturing vector mosquitoes of sylvatic arboviruses; however, occupational risks and biases due to individual skill and attractiveness are important limitations. The use of chemical attractants and automatic traps could be an alternative to resolve these limitations. This study compares the yields achieved using nets with those employing electrical traps with CO2 and BG-Lure®, near the ground and in the canopy strata (6.0 and 8.0 m high). The study was conducted at the Cantareira State Park, which is in the Brazilian Atlantic Forest biome. In the 18 collections performed, 3570 specimens of 52 taxa were obtained. The most frequent species captured near the ground were Wyeomyia confusa and Limatus durhamii, whereas Sabethes albiprivus, Sabethes purpureus, and Haemagogus leucocelaenus were the most frequent in the canopy. The nets resulted in greater species richness and abundance, followed by the trap employing CO2. The combination of CO2 traps with BG-Lure® did not improve performance. The use of BG-Lure® alone resulted in low abundance and a low number of species. Our results demonstrate that the use of traps with CO2 can be complementary to collections with nets; however, for species of epidemiological interest such as those of the genera Haemagogus and Sabethes, especially in the canopy, the net remains the method of choice.
RESUMO
Some ecological parameters and the distribution of vectors in the municipality of Eldorado, Vale do Ribeira Region, São Paulo, were studied. Entomological surveys were carried out from September 2019 to March 2021. It was observed that a few ecological parameters, including richness, abundance, diversity, and equitability, were typical of a modified environment, where artificial ecotopes maintain the presence of sandflies throughout the year. A total of 11,668 sandflies were captured. The presence of five taxa were observed in Eldorado, with low diversity and high dominance of Nyssomyia intermedia next to Ny. neivai, which are sympatric species. The results presented reinforce the importance of these species in anthropized areas in the transmission of cutaneous leishmaniasis (CL) agents and the need for entomological monitoring. Psathyromyia pascalei was encountered for the first time in the municipality, expanding the known area of distribution of this species in a modified environment.
RESUMO
Avian malaria parasites are widespread parasites transmitted by Culicidae insects belonging to different genera. Even though several studies have been conducted recently, there is still a lack of information about potential vectors of Plasmodium parasites, especially in Neotropical regions. Former studies with free-living and captive animals in São Paulo Zoo showed the presence of several Plasmodium and Haemoproteus species. In 2015, a pilot study was conducted at the zoo to collect mosquitoes in order to find out (i) which species of Culicidae are present in the study area, (ii) what are their blood meal sources, and (iii) to which Plasmodium species might they be potential vectors. Mosquitoes were morphologically and molecularly identified. Blood meal source and haemosporidian DNA were identified using molecular protocols. A total of 25 Culicidae species were identified, and 6 of them were positive for Plasmodium/Haemoproteus DNA. Ten mosquito species had their source of blood meal identified, which were mainly birds, including some species that were positive for haemosporidian parasites in the former study mentioned. This study allowed us to expand the list of potential vectors of avian malaria parasites and to improve our knowledge of the evolutionary and ecological relationships between the highly diverse communities of birds, parasites, and vectors present at São Paulo Zoo.
RESUMO
The effect of employing collars impregnated with deltamethrin 4% (DM4) to control canine visceral leishmaniasis (CVL) was evaluated. as were the individual factors associated with this infection. A cohort study that included household dogs was conducted between 2002 and 2006. The presence of pathognomonic signals, peridomiciliary sleep habits and breed were the main factors associated with the infection. The use of DM4 collars contributed to the reduction of CVL with an effectiveness of 66%, and the dogs' survival rate was greater than 90% at 50 months. In conclusion, the adoption of DM4 collars reduced the number of euthanized canines and in the incidence of CVL, and this reduction was sustained for one year after discontinuing the use of the collar.
RESUMO
Avaliou-se o hábito alimentar de Lutzomyia longipalpis em áreas de transmissão da leishmaniose visceral americana no município de Araçatuba (SP). De 1999 a 2001, capturas entomológicas mensais foram realizadas em três áreas do município, sendo pesquisadas amostras de 123 residências/área, durante 20 minutos/domicílio. As fêmeas ingurgitadas foram separadas e conservadas a -20ºC, até a realização do exame do conteúdo intestinal, pela reação de imunoprecipitação em tubo capilar. Utilizou-se anti-soros de: animais domésticos (cão, cavalo, gato e galinha), roedor e humano. Para a realização da reação, cada fêmea foi colocada em tubo com 0,2mL de solução fisiológica estéril mertiolada a 0,85%, por 12 horas. Posteriormente, foram maceradas e centrifugadas e 30ml do sobrenadante foram transferidos para o tubo capilar e adicionados 30ml de anti-soro. A reação foi dada como positiva quando observado grumo esbranquiçado até duas horas depois de seu início. Coletou-se 1.540 fêmeas de L. longipalpis, destas 699 estavam ingurgitadas quando coletadas e 613 (87,7%) foram submetidas ao teste, resultando em 45,6% de amostras reagentes. Das amostras reagentes 91,4% reagiram para sangue canino. Das 25 amostras reagentes para sangue humano 22 (88,0%) também reagiram para cão. Apenas 7,5% dos exemplares reagiram para sangue de ave. Em Araçatuba, L. longipalpis mostrou hábito eclético em relação à fonte alimentar, demonstrando alto grau de cinofilia. Embora as aves não tenham representado a principal fonte de alimento, deve-se atentar para o significado epidemiológico que elas representam, na medida em que permitem a domiciliação do vetor. Não foi observada mudança de hábito alimentar decorrente das atividades de controle vetorial.
Assuntos
Comportamento Alimentar , Leishmaniose Cutânea/transmissão , Leishmaniose Visceral/transmissão , Psychodidae , EpidemiologiaRESUMO
Este manual referente às atividades de vigilância e controle da leishmaniose visceral americana, incorporou todo conhecimento obtido com as revisões das atividades desenvolvidas deste 1999. Conforme já apresentamos anteriormente no II Informe Técnico: Leishmaniose Visceral Americana, entre outras alterações, modificou-se a classificação dos municípios, revisou-se o esquema de tratamento para casos humanos, o fluxo de notificação de casos humanos e caninos e a vigilância e o controle das populações canina e vetorial. Com este manual esperamos contribuir para uma melhor orientação dos técnicos da Secretaria Estadual da Saúde e dos municípios, para o desenvolvimento das atividades de vigilância e controle de doença.
Assuntos
Monitoramento Epidemiológico , Leishmaniose Cutânea , Leishmaniose Visceral , Saúde PúblicaRESUMO
Este manual referente às atividades de vigilância e controle da leishmaniose visceral americana, incorporou todo conhecimento obtido com as revisões das atividades desenvolvidas deste 1999. Conforme já apresentamos anteriormente no II Informe Técnico: Leishmaniose Visceral Americana, entre outras alterações, modificou-se a classificação dos municípios, revisou-se o esquema de tratamento para casos humanos, o fluxo de notificação de casos humanos e caninos e a vigilância e o controle das populações canina e vetorial. Com este manual esperamos contribuir para uma melhor orientação dos técnicos da Secretaria Estadual da Saúde e dos municípios, para o desenvolvimento das atividades de vigilância e controle de doença.