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1.
Argentinian j. respiratory physical therapy ; 5(2): 19-27, 2023. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1573883

RESUMO

RESUMEN Objetivo: Describir los cambios en la independencia y movilidad funcional en una muestra de niños con secuelas neurológicas secundarias a accidente cerebrovascular (ACV) subagudo. Materiales y método: Estudio observacional, descriptivo, retrospectivo y longitudinal. Se incluyeron sujetos de 4 a 18 años con diagnóstico de ACV subagudo internados en un centro de rehabilitación entre el 1 de febrero de 2005 y el 28 de febrero de 2023. Los datos de funcionalidad fueron evaluados al ingreso y egreso con la Escala de Independencia Funcional en Niños (WeeFIM, por sus siglas en inglés) y la Escala de Movilidad Funcional (FMS, por sus siglas en inglés). Resultados: Se analizaron 37 sujetos, de los cuales 17 (45,9%) presentaron ACV secundario a malformación arteriovenosa. Al comparar el puntaje de la FMS al ingreso y egreso, las diferencias resultaron estadísticamente significativas en las 3 distancias evaluadas (p<0,001). La mediana del puntaje de la escala WeeFIM fue de 46 (RIQ 36-55) al ingreso y de 86 (RIQ 74-95) al egreso [mediana de cambio 32,5 (RIQ 19-46) puntos; p<0,001]. Conclusión: En esta muestra de niños con ACV subagudo, se observaron cambios favorables en el porcentaje de independencia funcional y en la adquisición de la marcha independiente en distancias cortas, medias y largas.


ABSTRACT Objective: To describe changes in functional independence and mobility in a sample of children with neurological sequelae secondary to subacute stroke. Materials and method: This is an observational, descriptive, retrospective, and longitudinal study. Subjects aged 4 to 18 years diagnosed with subacute stroke admitted to a rehabilitation center between February 1, 2005, and February 28, 2023, were included. Functional data were evaluated at admission and discharge with the Functional Independence Measure for Children (WeeFIM) and the Functional Mobility Scale (FMS). Results: Thirty-seven subjects were analyzed; of them, 17 (45.9%) had stroke secondary to arteriovenous malformation. When comparing the FMS score at admission and discharge, the differences were statistically significant in the 3 distances evaluated (p<0.001). The median WeeFIM score was 46 (RIQ 36-55) at admission and 86 (RIQ 74-95) at discharge [median change 32.5 (RIQ 19-46) points; p<0.001]. Conclusion: In this sample of children with subacute stroke, favorable changes were observed in the percentage of functional independence and acquisition of independent walking in short, medium, and long distances.

2.
PLoS One ; 15(8): e0237667, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32833960

RESUMO

BACKGROUND AND AIMS: This is the first time that obesity and diabetes mellitus (DM) as protein conformational diseases (PCD) are reported in children and they are typically diagnosed too late, when ß-cell damage is evident. Here we wanted to investigate the level of naturally-ocurring or real (not synthetic) oligomeric aggregates of the human islet amyloid polypeptide (hIAPP) that we called RIAO in sera of pediatric patients with obesity and diabetes. We aimed to reduce the gap between basic biomedical research, clinical practice-health decision making and to explore whether RIAO work as a potential biomarker of early ß-cell damage. MATERIALS AND METHODS: We performed a multicentric collaborative, cross-sectional, analytical, ambispective and blinded study; the RIAO from pretreated samples (PTS) of sera of 146 pediatric patients with obesity or DM and 16 healthy children, were isolated, measured by sound indirect ELISA with novel anti-hIAPP cytotoxic oligomers polyclonal antibody (MEX1). We carried out morphological and functional studied and cluster-clinical data driven analysis. RESULTS: We demonstrated by western blot, Transmission Electron Microscopy and cell viability experiments that RIAO circulate in the blood and can be measured by ELISA; are elevated in serum of childhood obesity and diabetes; are neurotoxics and works as biomarkers of early ß-cell failure. We explored the range of evidence-based medicine clusters that included the RIAO level, which allowed us to classify and stratify the obesity patients with high cardiometabolic risk. CONCLUSIONS: RIAO level increases as the number of complications rises; RIAOs > 3.35 µg/ml is a predictor of changes in the current indicators of ß-cell damage. We proposed a novel physio-pathological pathway and shows that PCD affect not only elderly patients but also children. Here we reduced the gap between basic biomedical research, clinical practice and health decision making.


Assuntos
Diabetes Mellitus Tipo 1/patologia , Diabetes Mellitus Tipo 2/patologia , Células Secretoras de Insulina/patologia , Polipeptídeo Amiloide das Ilhotas Pancreáticas/metabolismo , Obesidade/patologia , Estrutura Quaternária de Proteína , Adolescente , Animais , Linhagem Celular , Sobrevivência Celular , Células Cultivadas , Criança , Pré-Escolar , Estudos Transversais , Diabetes Mellitus Tipo 1/sangue , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/complicações , Humanos , Polipeptídeo Amiloide das Ilhotas Pancreáticas/sangue , Polipeptídeo Amiloide das Ilhotas Pancreáticas/toxicidade , Polipeptídeo Amiloide das Ilhotas Pancreáticas/ultraestrutura , Microscopia Eletrônica de Transmissão , Neurônios/efeitos dos fármacos , Obesidade/sangue , Obesidade/complicações , Projetos Piloto , Cultura Primária de Células , Multimerização Proteica , Ratos , Testes de Toxicidade Aguda
5.
Vector Borne Zoonotic Dis ; 17(7): 495-502, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28530509

RESUMO

Toxoplasma gondii is a protozoan parasite with a broad ecological valence, which has been detected in a wide range of hosts and landscapes. Although the genus is considered monospecific, in recent years it has been demonstrated to exhibit more genetic variability than previously known. In Mexico, there are few genotyping studies, which suggest that classical, autochthonous, and atypical strains are circulating. The goal of this study was to describe T. gondii genetic diversity in naturally infected sheep from Colima, Mexico. This is a good site to study ecological aspects of this parasite since it is located between the Nearctic and Neotropical ecozones and it includes domestic and wild risks for transmission. We analyzed 305 tissue samples of semicaptive sheep from six coastal and central zones of Colima and border zones of Michoacán. We used an 803 bp amplicon of the B1 gene to genotype T. gondii and seroprevalence was determined by ELISA. Indexes for genetic diversity and genetic differentiation were calculated and compared with reference strains from North America (NA) and South America (SA). Twenty-three tissue samples were positive for the B1 gene by PCR, which were sequenced. Crude prevalence was 24.4%. The genetic analysis showed 16 variable sites along the 803 bp region that grouped all sequences into 13 haplotypes in the phylogenetic tree. Bayesian and haplotype network analysis showed nine new B1-types, of which three were frequent and six had unique alleles. Comparisons among sequence sets revealed that the Mexican population had lower differentiation than SA and an intermediate genetic variability between South America and North America. The B1 gene analysis showed new T. gondii haplotypes in naturally infected sheep; therefore, this marker could be initially used in molecular screening studies to identify potentially virulent genotypes of this parasite using natural host samples directly.


Assuntos
Genótipo , Proteínas de Protozoários/genética , Doenças dos Ovinos/parasitologia , Toxoplasma , Toxoplasmose Animal/parasitologia , Animais , Doenças Endêmicas , Feminino , Feto/virologia , Regulação da Expressão Gênica/fisiologia , Masculino , México/epidemiologia , Filogenia , Proteínas de Protozoários/metabolismo , Ovinos , Doenças dos Ovinos/epidemiologia , Toxoplasmose Animal/epidemiologia
6.
Vet Parasitol ; 209(1-2): 125-8, 2015 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-25737051

RESUMO

Toxoplasma gondii is among the commonest zoonotic infectious agents worldwide. It infects many warm-blooded animals, including felines, the definitive hosts. This parasite is now classified in 15 haplogroups spread out around the world. Few reports reveal a predominance of genotypes I and III in Mexico, although recombinant and atypical variants have also been found in humans and animals. The aim of this study was to detect, isolate and genotype T. gondii from cats of Colima Mexico, and to analyze tissue distribution of the parasite. IgG specific antibodies were investigated in 48 serum samples from unwanted and stray cats by indirect ELISA. Isolation in mice and molecular characterization by PCR-RFLP and sequencing were attempted using pools of brain, heart, liver, lung, spleen and brachiocephalic muscle samples of seropositive cats. Fourteen animals (29.2%) were seropositive, the frequency ranged between 27.3 and 40% among the different localities. Ten seropositive animals were euthanized, eight of them were positive for the B1 gene by conventional PCR. More frequently infected tissues were the brachiocephalic muscle (75.0%) the brain (63.0%) and the spleen (63.0%). Genotype III was determined for the SAG3 locus of the parasite infecting an unwanted cat. Tachyzoites were isolated from the peritoneal cavity of two mice inoculated with the tissue pool of one kitten. Type I alleles were found in SAG1, SAG2, SAG3, BTUB, GRA6, c29-2 and PK1 loci, while c22-8 was type II, and L358 and Apico were type III. This genotype corresponds to ToxoDB genotype #28. This is the first T. gondii isolate genetically characterized in Colima, Mexico and is different to other isolations of the country.


Assuntos
Doenças do Gato/parasitologia , Toxoplasma/genética , Toxoplasmose Animal/parasitologia , Animais , Bioensaio , Doenças do Gato/epidemiologia , Gatos , Regulação da Expressão Gênica/fisiologia , Genótipo , México/epidemiologia , Camundongos , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/epidemiologia
7.
Rev Invest Clin ; 65(4): 331-5, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24304734

RESUMO

INTRODUCTION: Recent studies concluded long-term training programs have improved antioxidant system in young and adults diabetic rats. However, to our knowledge, little attention has been paid to elderly individuals. OBJECTIVE: To assess the influence of a shorter training program in reducing oxidative damage in elderly diabetic rats. MATERIAL AND METHODS: Twenty-four male homozygous Zucker diabetic fatty rats (Gmi, fa/fa) aged 18-weeks with an average weight of 370- 450 g were purchased. After a 2-week period of environmental adaptation, animals were randomly distributed into exercised group (n = 12) that performed a 6-week swimming training protocol and sedentary group (n = 12). Animals were sacrificed 24-h after the last exercise session under anesthesia. Serum metabolic profile was determined. Lipid oxidation, expressed as malondialdehyde and protein oxidation, expressed as carbonyl groups, were assessed in plasma samples. This protocol was approved by an Institutional Ethics Committee. RESULTS: Exercised rats improved significantly their metabolic profile in comparison to controls. Plasma malondialdehyde (1.58 ± 0.39 vs. 2.06 ± 0.41 nmol/mL; p = 0.016) and carbonyl group levels (1.37 ± 0.33 vs. 1.62 ± 0.58 UA; p = 0.011) were also significantly lowered in exercised rats when compared to sedentary counterparts. CONCLUSIONS: A 6-week swimming training program reduced lipid and protein oxidation in elderly fatty diabetic rats. Further studies on this topic are required.


Assuntos
Estresse Oxidativo , Condicionamento Físico Animal , Fatores Etários , Animais , Masculino , Condicionamento Físico Animal/fisiologia , Ratos , Ratos Zucker , Fatores de Tempo
8.
Int. j. morphol ; 31(2): 570-574, jun. 2013. ilus
Artigo em Espanhol | LILACS | ID: lil-687103

RESUMO

En la actualidad se acepta que la obesidad es un grave problema de salud pública. La situación es aún más preocupante entre las personas con discapacidad intelectual, especialmente entre las mujeres. Afortunadamente recientes estudios sugieren la utilidad del ejercicio como estrategia frente a la obesidad. Sin embargo, la mayoría de estos estudios utilizan grupos mixtos (hombres y mujeres) o varones, siendo escasa la información sobre un grupo homogéneo de mujeres. Por consiguiente nos planteamos como hipótesis que un programa de entrenamiento de 10 semanas podría reducir el porcentaje de masa grasa y la distribución abdominal en mujeres obesas con síndrome de Down. En nuestro estudio participaron 20 mujeres adultas (18-30 años) con un cociente intelectual de 50-69 según la escala Stanford-Binet y diagnóstico citogenético de trisomía 21. Todas presentaban obesidad definida como IMC>30 kg/m2. Once de las participantes se asignaron aleatoriamente al grupo experimental y desarrollaron un programa de entrenamiento de 10 semanas de tipo aeróbico con 3 sesiones/semana. El porcentaje de masa grasa se determinó mediante impedanciometría bioeléctrica (Tanita TBF521). Para obtener el índice cintura/cadera se midieron las circunferencias de la cintura y la cadera utilizando una cinta antropométrica. Nuestro protocolo fue aprobado por un Comité de Ética Institucional. Nuestros resultados confirman que el ejercicio aeróbico redujo significativamente el porcentaje de masa grasa, el índice cintura/cadera y el perímetro de la cintura (p<0,05). Por el contrario no se observaron cambios en el grupo control. Concluimos que un programa de entrenamiento de 10 semanas consiguió mejorar la composición corporal de mujeres obesas con síndrome de Down. Futuros estudios longitudinales bien conducidos y controlados son necesarios para conocer el impacto de esta mejora en el manejo clínico de este grupo.


Recent studies have reported obesity prevalence in people with intellectual disability is even higher than in the general population what may finally lead to impair their health status and increase healthcare costs. Fortunately several studies have reported regular exercise may improve body composition in obese people with and without intellectual disability. To the best of our knowledge this is the first study conducted exclusively in female participants with intellectual disability, in an attempt to keep our sample homogeneous. To date, many studies focused on the influence of regular exercise in people with intellectual disability have recruited mixed (males and females) groups in order to increase their sample size to strengthen research designs. Therefore, we assessed the influence of a 10-week aerobic training program on fat mass percentage and indices of obesity in women with Down syndrome. To get this goal, twenty obese young women with Down syndrome volunteered for this study. Eleven were randomly assigned to perform a 10-week aerobic training program, 3 sessions/week, consisting of warming-up followed by a main part in a treadmill (30-40 min) at a work intensity of 55-65% of peak heart rate and a cooling-down period. Control group included 9 age, sex and BMI matched women with Down syndrome. Fat mass percentage and fat distribution were measured. This protocol was approved by an Institutional Ethics Committee. When compared to baseline, fat mass percentage, waist circumference and waist to hip ratio were significantly reduced after training. Conversely, no changes were reported in controls. It was concluded a 10-week training program reduced fat mass in obese adult women with Down syndrome.


Assuntos
Humanos , Adolescente , Adulto , Feminino , Adulto Jovem , Exercício Físico/fisiologia , Gordura Abdominal/anatomia & histologia , Deficiência Intelectual , Obesidade , Relação Cintura-Quadril , Índice de Massa Corporal
9.
Tuberculosis (Edinb) ; 84(1-2): 82-92, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-14670349

RESUMO

Tuberculoid (TT) and lepromatous leprosy (LL) develop in the human host depending on his ability to trigger a specific cellular immune response(CIR). Different genes have been demonstrated in susceptibility/protection and may explain the forms of leprosy. The major histocompatibility complex (MHC) play an important role. The aim of the study was to explore the contribution of human leukocyte antigen (HLA) DRB1, DQA1, DQB1 and DQ promoter genes in LL Mexican patients. Six families (26 LL, three TT patients and 27 controls) were analyzed; 114 unrelated patients were compared with 204 controls. Class I typing was done by the standard microlymphocytotoxicity and class II typing using PCR-SSOP. Haplotype segregation correlated with specific CIR in vivo and in vitro using lepromin. Haplotype sharing was significantly deviated in the affected sibs (p=0.01). Six healthy sibs were non-responders to lepromin and four of them were DQ1 homozgotes. DQ1 was significantly associated with LL and with non-responders. We set up macrophage activation experiments after infecting these cells with 5x10(6) bacilli to demonstrate if elimination occurred in the context or DQ1. When DQ1 was present on macrophages and on T cells, bacteria were poorly eliminated from the cell (32%) while when absent, 76% of the individuals were able to eliminate the bacilli (p=0.03). DRB1*1501 DQA1*0102-DQB1*0602 (DQ1 subtype) was significantly increased in the patients, indicating its participation in susceptibility. QBP 5.11/5.12 promoter present in the mentioned haplotype, and QAP 1.4, linked to DRB1*1301/02 haplotypes were also associated. Two mechanisms are suggested: the promoter polymorphisms may influence allele expression and thus the amount of peptides presented to the T-cell receptor, leading to a deficient CIR: HLA restriction is important for vaccine design; the way peptides anchor the DRB1*1501 groove may be relevant to the activation of TH1 cells, which contribute to an efficient presentation of peptides inducing a protective T-cell response.


Assuntos
Vacinas Bacterianas , Genes MHC da Classe II , Predisposição Genética para Doença , Hanseníase/genética , Vacinas Sintéticas , Humanos , Hanseníase/prevenção & controle
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