RESUMO
OBJECTIVES: The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue infection were studied. DESIGN: Serum, saliva, and urine samples were collected from 22 patients with clinical and confirmed dengue 3 virus infection during the outbreak in Havana City in 2001. They were tested by capture IgM (MAC-ELISA), IgA (AAC-ELISA), and IgE (EAC-ELISA) and IgG ELISA inhibition method (EIM) to detect specific dengue antibodies. RESULTS: Similar kinetics were observed in IgM, IgA, and IgG antibodies in saliva and IgA and IgG in urine samples from secondary cases compared with kinetics in serum samples, although the values were lower. No IgG antibody was detected in saliva and urine samples in primary cases and IgM antibody was not detected in urine samples from either primary or secondary infection. All secondary cases were positive for IgG in saliva and urine samples at day 7. The kinetics of specific IgE antibodies in primary and secondary cases were different. CONCLUSIONS: The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infection were studied for the first time, showing its behavior and usefulness in dengue virus diagnosis. The specific IgE could play a role as a serological marker in secondary infections.
Assuntos
Anticorpos Antivirais/análise , Vírus da Dengue/imunologia , Dengue/diagnóstico , Dengue/imunologia , Adulto , Anticorpos Antivirais/sangue , Anticorpos Antivirais/urina , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina E/análise , Imunoglobulina E/sangue , Imunoglobulina E/urina , Imunoglobulina G/análise , Imunoglobulina G/sangue , Imunoglobulina G/urina , Imunoglobulina M/análise , Imunoglobulina M/sangue , Imunoglobulina M/urina , Cinética , Masculino , Pessoa de Meia-Idade , Saliva/imunologiaRESUMO
BACKGROUND: The detection of the IgM antibody for the dengue virus in serum by ELISA has become one of the most important and useful methods for diagnosis of dengue using a single acute-phase serum sample. Currently, this system is an invaluable tool for the surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF). The usefulness of other serological markers such as IgA and IgE have been less studied. OBJECTIVE: To study the IgM, IgA and IgE specific antibody response in dengue 3 infected patients with different clinical picture and type of infection. STUDY DESIGN: One hundred and twenty-seven serum samples collected on days 5-7 at the onset of fever from clinically and serologically confirmed dengue cases were studied. Forty-two were classified as primary dengue fever cases, 48 as secondary dengue fever cases and 37 as secondary dengue hemorrhagic fever cases. All samples were tested by capture ELISA in order to detect dengue IgM, IgA and IgE antibodies. RESULTS AND CONCLUSIONS: In this study, significant differences were observed in the IgM, IgA and IgE response between the study groups. High IgA and IgE OD ratios in secondary dengue cases were found. The usefulness of serotype specific IgM antibody detection is also analyzed and discussed. A priority for future dengue research in terms of protection, recovery of infection and immunopathogenesis is to elucidate the role of these immunoglobulins. The cross reactivity response to IgM between dengue virus serotypes in primary and secondary cases should also be more studied.
Assuntos
Anticorpos Antivirais/sangue , Vírus da Dengue/imunologia , Dengue/imunologia , Dengue Grave/imunologia , Biomarcadores , Cuba , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina A/sangue , Imunoglobulina E/sangue , Imunoglobulina M/sangueRESUMO
This article summarise our experience with the Beta-Blocker in the management of congestive heart failure, which did not respond to the usual medical treatment
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Carbazóis/uso terapêutico , Insuficiência Cardíaca/tratamento farmacológico , Metoprolol/uso terapêutico , Propanolaminas/uso terapêutico , Antagonistas Adrenérgicos beta/uso terapêuticoRESUMO
This article summarise our experience with the Beta-Blocker in the management of congestive heart failure, which did not respond to the usual medical treatment.