RESUMO
The diversity of Actinobacteria associated with Paratrechina longicornis, an ant species that prefers a high protein diet, in a subtropical dry forest (Guánica, Puerto Rico) was determined by culture methods and by 16S rDNA clone libraries. The results of both methodologies were integrated to obtain a broader view of the diversity. Streptomyces, Actinomadura, Nocardia, Ornithinimicrobium, Tsukamurella, Brevibacterium, Saccharopolyspora, Nocardioides, Microbacterium, Leifsonia, Pseudonocardia, Corynebacterium, Geodermatophilus, Amycolatopsis, and Nonomuraea were found associated with the ants. The genera Streptomyces and Actinomadura were the most abundant. Also, the diversity of Actinobacteria associated with the soil surrounding the nest was determined using 16S rDNA clone libraries. In total, 27 genera of Actinobacteria were associated with the nest soils. A dominant genus was not observed in any of the soil samples. We compared statistically the Actinobacteria communities among P. longicornis nests and each nest with its surrounding soil using the clone libraries data. We established that the communities associated with the ants were consistent and significantly different from those found in the soil in which the ants live.
Assuntos
Actinobacteria/classificação , Actinobacteria/genética , Actinobacteria/isolamento & purificação , Formigas/microbiologia , Variação Genética , Microbiologia do Solo , Animais , Biodiversidade , Brevibacterium/genética , Brevibacterium/isolamento & purificação , Corynebacterium/genética , Corynebacterium/isolamento & purificação , Florestas , Biblioteca Gênica , Nocardia/isolamento & purificação , Filogenia , Porto Rico , RNA Ribossômico 16S/genética , Saccharopolyspora/genética , Saccharopolyspora/isolamento & purificação , Análise de Sequência de DNA , Streptomyces/genética , Streptomyces/isolamento & purificaçãoRESUMO
In Puerto Rico the protist Enterobryus halophilus (Ichthyosporea: Eccrinales) has been reported associated with a new host, Emerita portoricensis (Malacostraca: Hippidae). A study on prevalence and abundance in populations of E. halophilus was conducted during 18 mo to determine whether sex-dependent infection exists in Em. portoricensis, as suggested (1958) for the original description of E. halophilus in Em. talpoida. Individuals (1440) of Em. portoricensis were collected and their guts dissected on microscope slides. The thalli of E. halophilus were counted under a microscope with random grid squares. A factorial ANOVA with interaction revealed that the prevalence and the abundance of E. halophilus do not depend on the sex of its host. Furthermore the data indicate that E. halophilus populations undergo seasonal variations in infestation following the population dynamics of the host.
Assuntos
Anomuros/parasitologia , Mesomycetozoea/fisiologia , Análise de Variância , Animais , Feminino , Interações Hospedeiro-Parasita , Modelos Logísticos , Masculino , Dinâmica Populacional , Prevalência , Porto Rico , Estações do Ano , Fatores Sexuais , SimbioseRESUMO
We report on the species of Harpellales found in dipteran hosts during two surveys (32 field d) in the state of Veracruz, Mexico. One new morphospecies, Genistellospora dorsicaudata, is described with particular attention to the position of the terminal cell associated with fully developed fertile thalli bearing sexual spores. We emend the description of G. guanacastensis to include morphometrics on the zygospores, based on discovery of the sexual spores for that species in our collections. Thirteen other previously described species, which are new for Mexico, include G. homothallica, Pennella montana, Simuliomyces microsporus, Smittium aciculare, S. brasiliense (in a new host type), S. culisetae, S. dipterorum, S. microsporum, S. simulii and the unbranched species Harpella melusinae, H. tica, Stachylina grandispora and S. paucispora. Some species have been described but not named, specifically one each of Harpella, Pennella and Smittium. All taxa are identified morphologically, illustrated and additional details on their ecology are provided.
Assuntos
Dípteros/microbiologia , Fungos/classificação , Animais , Fungos/citologia , Fungos/fisiologia , Larva/microbiologia , México , Esporos Fúngicos/classificação , Esporos Fúngicos/citologia , Árvores , Clima TropicalRESUMO
We present the first report of Harpellales (Kickxellomycotina) from Hispaniola. The Dominican Republic was surveyed, and members of the ecological group trichomycetes were encountered in the gut of their arthropod hosts. Among Harpellales we describe a new species, Smittium caribense, and report the presence of seven previously known species that are new for Hispaniola: Genistellospora homothallica, Harpella amazonica, Harpella tica, Legerio-m myces rarus, Smittium culisetae, Smittium dipterorum and Stachylina grandispora. The dominance of cosmopolitan, dipteran-associated species is the most outstanding effect resulting from the insularity on gut fungi.
Assuntos
Fungos/isolamento & purificação , Insetos/microbiologia , Animais , Biodiversidade , República Dominicana , Fungos/ultraestrutura , Geografia , Microscopia ConfocalRESUMO
This is the first survey of trichomycetes for Vieques, Puerto Rico. Several types of arthropods, including millipedes, crabs, mosquitoes, bloodworms, amphipods and isopods, were collected from marine, freshwater and terrestrial habitats and their guts examined for the presence of trichomycetes. A new species of Enterobryus, which was found attached to the gut of the millipede Anadenobolus arboreus (Diplopoda), is described as Enterobryus viequensis sp. nov. We report the presence of Smittium culisetae in mosquito larvae and also provide information on two eccrinids associated with the fiddler crabs Uca rapax and U. burgersi and an amphipod.
Assuntos
Artrópodes/microbiologia , Fungos/isolamento & purificação , Trato Gastrointestinal/microbiologia , Animais , Coleta de Dados , Ecossistema , Fungos/citologia , Porto Rico , Simbiose , Microbiologia da ÁguaRESUMO
We report the inedited occurrence of zygospores in symbiotic Asellariales (Zygomycota) from Puerto Rico and Dominican Republic. Two new species of Asellaria are described, Asellaria dactylopus and A. jatibonicua, both found in the hindgut of terrestrial isopods. The presence of spherical zygospores in the latter species represents a novelty for the order. The related gut-fungi Harpellales were known to produce sexual spores, characteristically conical or biconical. Order Asellariales is emended to include the description of zygospores. An undetermined species of Orchesellaria also is reported from the Caribbean. Comments are provided for each of the species as well as drawings and photographs to illustrate each taxon.
Assuntos
Fungos/isolamento & purificação , Esporos Fúngicos/citologia , Esporos Fúngicos/isolamento & purificação , Animais , República Dominicana , Fungos/citologia , Isópodes/microbiologia , Porto RicoRESUMO
This is the first report of Harpellales (Zygomycota) from Mexico, including herein only the endosymbiotic species of gut fungi in the digestive tracts or shed exuviae of Plecopteran and Ephemeropteran nymphs. Four new species are described: Allantomyces zopilotei, Bojamyces olmecensis, Gauthier-omyces viviparus and Graminella ophiuroidea. Among previously known Harpellales, Lancisporomyces nemouridarum and Zygopolaris ephemeridarum are southern range extremes and new records for Mexico. All species are illustrated and discussed relating to biogeographic implications of the new reports from Mexico, as well as the particular environmental circumstances of the Harpellales in the tropics.