RESUMO
In this letter, we describe the development of an immunodiagnostic assay capable of detecting specific IgM in the blood circulation of experimental Nile tilapia that received the vaccine against S. agalactiae.
Assuntos
Animais , Anticorpos , Ciclídeos/imunologia , Ciclídeos/sangue , Imunoglobulina M/análise , Imunoglobulina M/sangue , Técnicas ImunoenzimáticasRESUMO
In this letter, we describe the development of an immunodiagnostic assay capable of detecting specific IgM in the blood circulation of experimental Nile tilapia that received the vaccine against S. agalactiae.
RESUMO
In this letter, we describe the development of an immunodiagnostic assay capable of detecting specific IgM in the blood circulation of experimental Nile tilapia that received the vaccine against S. agalactiae.(AU)
Assuntos
Animais , Ciclídeos/sangue , Ciclídeos/imunologia , Anticorpos , Imunoglobulina M/análise , Imunoglobulina M/sangue , Técnicas ImunoenzimáticasAssuntos
Aconselhamento/métodos , Deficiência do Fator X/diagnóstico , Adulto , Feminino , Humanos , Gravidez , Estudos RetrospectivosRESUMO
Lachesis muta muta and Bothrops atrox snakes are responsible for most accidents occurring in the Amazon. The clinical features of the accidents are similar; however, there are still controversies about the efficacy of Bothrops antivenoms for treating L. m. muta accidents. In this work, we evaluated the antigenic cross-reactivity between these venoms using polyclonal and monoclonal antibodies and the efficacy of B. atrox and L. m. muta experimental antivenoms in cross-neutralizing the main toxic activities of each venom. Electrophoretic patterns differed consistently between the species. However, antigenic cross-reactivity was extensive except for a few bands. Several species-specific monoclonal antibodies were obtained by immunization of Balb/c mice with L. m. muta whole venom or B. atrox and L. m. muta specific antigens. The monoclonal antibodies specific to L. m. muta recognized different bands of this venom and the antibodies specific to B. atrox recognized a complex pattern on whole venom by Western blotting. These antibodies are important tools for developing an immunoassay able to discriminate patients bitten by these snakes. The experiments involving cross-neutralization of the main activities of the venoms showed that hemorrhage and blood incoagulability induced by B. atrox venom were similarly neutralized by both B. atrox and L. m. muta antivenoms. However, B. atrox antivenom partially neutralized the hemorrhage and completely failed in neutralizing coagulopathy induced by L. m. muta venom. Therefore, antigenic variation between B. atrox and L. m. muta venoms does occur and the use of specific antivenom is suggested for patients bitten by Lachesis snakes.