RESUMO
Genus Gammapapillomavirus (Gamma-PV) is the most diverse and largest clade within the Papillomaviridae family. A novel set of degenerate primers targeting the E1 gene was designed and further used in combination with the well-known CUT PCR assay to assess HPV prevalence and genus distribution in a variety of cutaneous samples from 448 immunocompetent individuals. General HPV, Gamma-PV and mixed infections prevalence were significantly higher in actinic keratosis with respect to benign and malignant neoplasms, respectively (pâ¯=â¯0.0047, pâ¯=â¯0.0172, pâ¯=â¯0.00001). Gamma-PVs were significantly more common in actinic keratosis biopsies than Beta- and Alpha-PVs (pâ¯=â¯0.002). The full-length genome sequence of a novel putative Gamma-PV type was amplified by 'hanging droplet' long-range PCR and cloned. The novel virus, designated HPV210, clustered within species Gamma-12. This study provides an additional tool enabling detection of HPV infections in skin and adds new insights about possible early roles of Gamma-PVs in the development of cutaneous malignant lesions.
Assuntos
Gammapapillomavirus/genética , Gammapapillomavirus/isolamento & purificação , Ceratose Actínica/virologia , Reação em Cadeia da Polimerase/métodos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Gammapapillomavirus/classificação , Humanos , Masculino , Pessoa de Meia-Idade , Infecções por Papillomavirus/virologia , Adulto JovemRESUMO
We present the first longitudinal study reporting the natural history of human papillomavirus (HPV) infection in sun-exposed skin of healthy individuals living in a geographical area in which solar UV radiation is influenced by the ozone content of the atmosphere. During three climatic seasons, skin swab samples were obtained from 78 healthy individuals and the prevalence of cutaneous HPVs was assessed with broad-spectrum FAP and CUT primers and determined at 54, 45 and 47â% in spring, summer and winter, respectively. Frequencies of mixed HPV infections were significantly higher in spring with respect to summer and winter (P=0.02). Seventy-one different HPV types/putative types were identified. While 62 volunteers were HPV-infected in at least one season, 23 had persistent infections. ß-PVs (ß-1) were the most prevalent and persistent. Age was associated with both the infection status (P=0.01) and the type of HPV infection (no infection, indeterminate/transient, persistent P=0.02). The molecular/phylogenetic analysis of the newly identified ß-PV, officially designated as HPV209, showed that the virus has a typical genomic organization of cutaneous HPVs with five early (E6, E7, E1, E2 and E4) and two late genes (L2 and L1), which clusters to the species ß-2. This provides useful data on cutaneous HPV infections in high UV-exposed regions.
Assuntos
Betapapillomavirus/classificação , Betapapillomavirus/isolamento & purificação , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/virologia , Pele/efeitos da radiação , Pele/virologia , Adulto , Análise por Conglomerados , DNA Viral/química , DNA Viral/genética , Feminino , Genótipo , Voluntários Saudáveis , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Filogenia , Prevalência , Estações do Ano , Análise de Sequência de DNA , Luz Solar , Raios UltravioletaRESUMO
Se describe una paciente con sarcoidosis cutánea crónica localizada en región frontotemporal derecha que se extiende al pabellón auricular, de diez años de evolución. La sarcoidosis cutánea es llamada la gran imitadora por sus similitudes clínicas con otras patologías cutáneas. La paciente refiere haber realizado múltiples tratamientos con corticoides tópicos, antibióticos tópicos, metronidazol tópico e isotretinoina vía oral, sin respuesta a los mismos. Se establece tratamiento local con tacrolimus 0.1% (dos veces al día) con escasa respuesta, observándose buena respuesta clínica luego de la administración sistémica de hidroxicloroquina 200 mg/d vía oral. El objetivo de esta publicación radica en describir una paciente con una forma cutánea pura de sarcoidosis facial al momento del diagnóstico, con buena respuesta a la hidroxicloroquina oral y efectuar una revisión bibliográfica de la terapéutica actual.