Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 18 de 18
Filtrar
Mais filtros











Intervalo de ano de publicação
1.
Anim Reprod ; 17(2): e20190115, 2020 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-32714451

RESUMO

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.

2.
Anim. Reprod. (Online) ; 17(2): e20190115, 2020. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1461504

RESUMO

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.


Assuntos
Masculino , Feminino , Animais , Camundongos , Criopreservação , Fase Folicular , Ruminantes/anatomia & histologia , Ruminantes/fisiologia , Vitrificação , Transplante Heterólogo
3.
Anim. Reprod. ; 17(2): e20190115, 2020. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-29308

RESUMO

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.(AU)


Assuntos
Animais , Masculino , Feminino , Camundongos , Ruminantes/anatomia & histologia , Ruminantes/fisiologia , Vitrificação , Fase Folicular , Criopreservação , Transplante Heterólogo
4.
Acta sci. vet. (Impr.) ; 46: Pub.1603-2018. tab
Artigo em Português | VETINDEX | ID: biblio-1457893

RESUMO

Background: The emergence of the NUDE and SCID immunosuppressed mice lineages generated knowledge on various mechanisms of lymphocyte maturation and human autoimmune diseases. Information on haematological and biochemical parameters of these lineages is still scarce, making it impossible to infer homeostasis by comparing data, or to detect genetic influences on the parameters for these species. Haematological and biochemical tests were carried out on Balb/c NUDE and C57BL/6 SCID mice of both sexes, aiming to analyse the presence of genetic influence on possible variations of such parameters and to verify reference values for both lineages.Materials, Methods & Results: One hundred and forty mice (Mus musculus) of the Balb/C NUDE and C57BL/6 SCID lineages were used in the present study. The animals were previously anesthetized, the blood collection procedure was performed by cardiac puncture and the samples were collected in the presence of heparin and intended for haematological and biochemical evaluation, under standardized conditions. The haematological evaluation consisted of red blood cell count, leukocyte counts, platelet counts, haematocrit, haemoglobin concentration, mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC). The quantified biochemical parameters were: urea, creatinine, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP). While analysing the obtained data, it was possible to observe that only females presented divergences (P < 0.05) in the red blood cell series, in haemoglobin and in mean haemoglobin concentration (MCH). Regarding the analysis of the white blood cell series, females only presented differences (P < 0.05) in the leukocyte count. For males, there were variations (P < 0.05) in the counts of leukocytes, eosinophils, lymphocytes and neutrophils.[...]


Assuntos
Animais , Camundongos , Biomarcadores/análise , Terapia de Imunossupressão , Variação Genética/imunologia , Testes Hematológicos/métodos , Testes Hematológicos/veterinária
5.
Acta sci. vet. (Online) ; 46: Pub. 1603, 2018. tab
Artigo em Português | VETINDEX | ID: vti-735401

RESUMO

Background: The emergence of the NUDE and SCID immunosuppressed mice lineages generated knowledge on various mechanisms of lymphocyte maturation and human autoimmune diseases. Information on haematological and biochemical parameters of these lineages is still scarce, making it impossible to infer homeostasis by comparing data, or to detect genetic influences on the parameters for these species. Haematological and biochemical tests were carried out on Balb/c NUDE and C57BL/6 SCID mice of both sexes, aiming to analyse the presence of genetic influence on possible variations of such parameters and to verify reference values for both lineages.Materials, Methods & Results: One hundred and forty mice (Mus musculus) of the Balb/C NUDE and C57BL/6 SCID lineages were used in the present study. The animals were previously anesthetized, the blood collection procedure was performed by cardiac puncture and the samples were collected in the presence of heparin and intended for haematological and biochemical evaluation, under standardized conditions. The haematological evaluation consisted of red blood cell count, leukocyte counts, platelet counts, haematocrit, haemoglobin concentration, mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC). The quantified biochemical parameters were: urea, creatinine, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP). While analysing the obtained data, it was possible to observe that only females presented divergences (P < 0.05) in the red blood cell series, in haemoglobin and in mean haemoglobin concentration (MCH). Regarding the analysis of the white blood cell series, females only presented differences (P < 0.05) in the leukocyte count. For males, there were variations (P < 0.05) in the counts of leukocytes, eosinophils, lymphocytes and neutrophils.[...](AU)


Assuntos
Animais , Camundongos , Terapia de Imunossupressão , Biomarcadores/análise , Variação Genética/imunologia , Testes Hematológicos/métodos , Testes Hematológicos/veterinária
6.
Semina Ci. agr. ; 38(3): 1323-1334, maio-jun. 2017. graf, tab
Artigo em Inglês | VETINDEX | ID: vti-13669

RESUMO

Trypanosoma vivax is a protozoan originating from the African continent, which, although it has not yet been able to complete its biological cycle in South America, due to the absence of the tsetse fly, can still cause death in ruminants. The objective of this study was to verify the effects of T. vivax on the measurements and indices in sheep that characterize animal performance, as well as on economic losses in meat animals. Twenty intact adult male sheep were used for this study, all of approximately the same ages and weights, reared in confinement, and subjected to the same management and diet, which was balanced and supplemented with adequate minerals. The animals were divided into two groups: the control group (CG) and the infected group (IG), which was inoculated intravenously with 1.3 x 105 trypomastigotes of T. vivax. Feed intake was verified daily, whereas the feed conversion (FC), feed efficiency index (FEI), and weight gain were obtained weekly. Total weight gain (TWG) was determined after 70 days post-infection. The economic loss was calculated by subtracting the value obtained (IG) from the expected value (CG), and the difference was expressed as a percentage. A randomized block design was used to isolate the effect of the initial weight. The means were compared by the Student “t” test at 5%. Of the 10 infected animals, one died from the parasitism, yielding a rate much lower than that observed in natural outbreaks. The groups presented similar feed intakes throughout the experimental period; however, the TWG of the infected group was significantly lower (50.7%) than that of the CG. Similarly, the daily weight gain (DWG), feed conversion (FC), and feed efficiency index (FEI) of the IG were significantly lower than those of the CG. In addition, the worst rates of FC and FEI coincided with parasitemia peaks and recurrences, probably due to immunological demand and tissue repair...(AU)


O Trypanosoma vivax é um protozoário originário do Continente Africano que mesmo não completando seu ciclo biológico na América do Sul, devido à ausência da mosca tsé-tsé, pode levar ruminantes a óbito. O presente trabalho teve por objetivo verificar, em ovinos infectados, os efeitos do T. vivax em medidas e índices que caracterizam o desempenho animal, bem como perdas econômicas em animais de abate. Para tanto foram utilizados vinte ovinos adultos, machos, inteiros com idade e peso aproximados, criados em confinamento, submetidos ao mesmo manejo e alimentação, sendo esta balanceada e suplementada com minerais adequados. Os animais foram divididos em dois grupos: o grupo controle (GC) e o grupo infectado (GI) que foi alcançado pela inoculação endovenosa de 1,3 x 105 tripomastigotas de T. vivax. O consumo de ração foi verificado diariamente, enquanto a conversão alimentar (CA), o índice de eficiência alimentar (IEA) e o ganho de peso foram obtidos semanalmente. O ganho de peso total (GPT) foi obtido ao final de 70 dias pós-infecção. A perda econômica foi obtida subtraindo do valor esperado (GC) do valor obtido (GI), e a diferença exposta em porcentagem. O delineamento utilizado foi o de blocos casualizados, para isolar o efeito do peso inicial, foi aplicado o teste “t” de Student a 5% para comparação de médias. A taxa de letalidade do T. vivax em ambiente experimental controlado foi de 10%, taxa muito inferior ao verificado em surtos naturais. Quanto ao consumo de ração, os grupos apresentaram consumo idêntico durante todo o período, todavia, o GPT do grupo infectado foi significativamente inferior, 50,7% menor quando comparado ao GC. Do mesmo modo, o ganho de peso diário (GPD), conversão alimentar (CA) e o índice de eficiência alimentar (IEA) do GI foram significantemente inferiores ao GC. Além disso, os piores índices de CA e IEA coincidiram com os picos parasitêmicos e recidivas, provavelmente pela demanda imunológica, e de reparos teciduais...(AU)


Assuntos
Animais , Ovinos/crescimento & desenvolvimento , Ração Animal , Trypanosoma vivax/parasitologia
7.
Semina ciênc. agrar ; 38(3): 1323-1334, maio-jun. 2017. graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1500783

RESUMO

Trypanosoma vivax is a protozoan originating from the African continent, which, although it has not yet been able to complete its biological cycle in South America, due to the absence of the tsetse fly, can still cause death in ruminants. The objective of this study was to verify the effects of T. vivax on the measurements and indices in sheep that characterize animal performance, as well as on economic losses in meat animals. Twenty intact adult male sheep were used for this study, all of approximately the same ages and weights, reared in confinement, and subjected to the same management and diet, which was balanced and supplemented with adequate minerals. The animals were divided into two groups: the control group (CG) and the infected group (IG), which was inoculated intravenously with 1.3 x 105 trypomastigotes of T. vivax. Feed intake was verified daily, whereas the feed conversion (FC), feed efficiency index (FEI), and weight gain were obtained weekly. Total weight gain (TWG) was determined after 70 days post-infection. The economic loss was calculated by subtracting the value obtained (IG) from the expected value (CG), and the difference was expressed as a percentage. A randomized block design was used to isolate the effect of the initial weight. The means were compared by the Student “t” test at 5%. Of the 10 infected animals, one died from the parasitism, yielding a rate much lower than that observed in natural outbreaks. The groups presented similar feed intakes throughout the experimental period; however, the TWG of the infected group was significantly lower (50.7%) than that of the CG. Similarly, the daily weight gain (DWG), feed conversion (FC), and feed efficiency index (FEI) of the IG were significantly lower than those of the CG. In addition, the worst rates of FC and FEI coincided with parasitemia peaks and recurrences, probably due to immunological demand and tissue repair...


O Trypanosoma vivax é um protozoário originário do Continente Africano que mesmo não completando seu ciclo biológico na América do Sul, devido à ausência da mosca tsé-tsé, pode levar ruminantes a óbito. O presente trabalho teve por objetivo verificar, em ovinos infectados, os efeitos do T. vivax em medidas e índices que caracterizam o desempenho animal, bem como perdas econômicas em animais de abate. Para tanto foram utilizados vinte ovinos adultos, machos, inteiros com idade e peso aproximados, criados em confinamento, submetidos ao mesmo manejo e alimentação, sendo esta balanceada e suplementada com minerais adequados. Os animais foram divididos em dois grupos: o grupo controle (GC) e o grupo infectado (GI) que foi alcançado pela inoculação endovenosa de 1,3 x 105 tripomastigotas de T. vivax. O consumo de ração foi verificado diariamente, enquanto a conversão alimentar (CA), o índice de eficiência alimentar (IEA) e o ganho de peso foram obtidos semanalmente. O ganho de peso total (GPT) foi obtido ao final de 70 dias pós-infecção. A perda econômica foi obtida subtraindo do valor esperado (GC) do valor obtido (GI), e a diferença exposta em porcentagem. O delineamento utilizado foi o de blocos casualizados, para isolar o efeito do peso inicial, foi aplicado o teste “t” de Student a 5% para comparação de médias. A taxa de letalidade do T. vivax em ambiente experimental controlado foi de 10%, taxa muito inferior ao verificado em surtos naturais. Quanto ao consumo de ração, os grupos apresentaram consumo idêntico durante todo o período, todavia, o GPT do grupo infectado foi significativamente inferior, 50,7% menor quando comparado ao GC. Do mesmo modo, o ganho de peso diário (GPD), conversão alimentar (CA) e o índice de eficiência alimentar (IEA) do GI foram significantemente inferiores ao GC. Além disso, os piores índices de CA e IEA coincidiram com os picos parasitêmicos e recidivas, provavelmente pela demanda imunológica, e de reparos teciduais...


Assuntos
Animais , Ovinos/crescimento & desenvolvimento , Ração Animal , Trypanosoma vivax/parasitologia
8.
Acta sci. vet. (Online) ; 45: 1-5, 2017. tab
Artigo em Português | VETINDEX | ID: vti-20219

RESUMO

Background: Animal models are widely used in scientific research because of the ability to generate information from an organism like everything under a given experimental condition. Hematological and biochemical tests in laboratory animals are essential for the validation of several scientific studies. In addition, it standardizes physiological values for these animals according to their sex, age, lineage, environment, and nutritional status. The present work aims to establish reference values for biochemical and hematological standards in Balb/c mice, for males and females.Materials, Methods & Results: A total of 50 male and female mice were used at reproductive age. The procedures for collecting, processing, and analyzing the samples were standardized. The collected blood samples were immediately transferred to eppendorf tubes containing heparin, and intended for hematological and biochemical evaluation. The hematological evaluation consisted of Red blood cell count (RBC), Leukocyte counts (WBC), Platelet counts (PLT), Hematocrit (HCT), Hemoglobin concentration (HGB), Mean corpuscular volume (MCV), and Mean corpuscular hemoglobin concentration (MCHC). Already the quantified biochemical parameters were: urea, creatinine, alanina aminotransaminase (ALT), aspartato aminotransaminase (AST) and fosfatase alcalina (FAL). The differential leukocyte count was also performed. Hematological results obtained for males and females were: 9.19 ± 3.35 (106/mm³) and 7.3 ± 2.01(106/mm³) of RBC; 35.8 ± 6.7% and 38.44 ± 3.93% of HCT; 11.51 ± 2.17 g/dL and 11.85 ± 1.56 g/dL of HGB; 45.83 ± 15.03 fL and 60.26 ± 18.25 fL of VCM; 31.80 ± 1.15% and 31.88 ± 0.99% of MCHC; and, 5380 ± 1994.21(10³/mm³) and 3564 ± 1071(10³/mm³) of WBC. The platelet counts were 878.92 ± 84.19 and 678.28 ± 227.21, for males and females respectively.[...](AU)


Assuntos
Animais , Camundongos , Camundongos Endogâmicos BALB C/sangue , Animais de Laboratório , Contagem de Células Sanguíneas/veterinária , Testes Hematológicos/veterinária , Valores de Referência
9.
Acta sci. vet. (Impr.) ; 45: 1-5, 2017. tab
Artigo em Português | VETINDEX | ID: biblio-1457647

RESUMO

Background: Animal models are widely used in scientific research because of the ability to generate information from an organism like everything under a given experimental condition. Hematological and biochemical tests in laboratory animals are essential for the validation of several scientific studies. In addition, it standardizes physiological values for these animals according to their sex, age, lineage, environment, and nutritional status. The present work aims to establish reference values for biochemical and hematological standards in Balb/c mice, for males and females.Materials, Methods & Results: A total of 50 male and female mice were used at reproductive age. The procedures for collecting, processing, and analyzing the samples were standardized. The collected blood samples were immediately transferred to eppendorf tubes containing heparin, and intended for hematological and biochemical evaluation. The hematological evaluation consisted of Red blood cell count (RBC), Leukocyte counts (WBC), Platelet counts (PLT), Hematocrit (HCT), Hemoglobin concentration (HGB), Mean corpuscular volume (MCV), and Mean corpuscular hemoglobin concentration (MCHC). Already the quantified biochemical parameters were: urea, creatinine, alanina aminotransaminase (ALT), aspartato aminotransaminase (AST) and fosfatase alcalina (FAL). The differential leukocyte count was also performed. Hematological results obtained for males and females were: 9.19 ± 3.35 (106/mm³) and 7.3 ± 2.01(106/mm³) of RBC; 35.8 ± 6.7% and 38.44 ± 3.93% of HCT; 11.51 ± 2.17 g/dL and 11.85 ± 1.56 g/dL of HGB; 45.83 ± 15.03 fL and 60.26 ± 18.25 fL of VCM; 31.80 ± 1.15% and 31.88 ± 0.99% of MCHC; and, 5380 ± 1994.21(10³/mm³) and 3564 ± 1071(10³/mm³) of WBC. The platelet counts were 878.92 ± 84.19 and 678.28 ± 227.21, for males and females respectively.[...]


Assuntos
Animais , Camundongos , Animais de Laboratório , Camundongos Endogâmicos BALB C/sangue , Contagem de Células Sanguíneas/veterinária , Testes Hematológicos/veterinária , Valores de Referência
10.
Acta Vet. Brasilica ; 10(1): 98-103, 2016. ilus, tab
Artigo em Português | VETINDEX | ID: biblio-1453001

RESUMO

The aim of this study was to compare two receptor sites in ovarian autologous transplantation in mice. Twelve Balb-c mice were randomly divided into three groups of four animals each: G1 - non-transplanted animals, G2 - animals which received the ovarian fragment in the perimetrium, G3 - animals which had the abdominal subcutaneous tissue as a receptor site. For transplantation, mice were subjected to bilateral salpingectomia. The left ovary was submitted for histological processing. The right ovarian cortex was fragmented and reimplanted in the specified places according to the groups. Transplants were recovered in 3/4 of the mice in each treatment group. In vaginal washes, the return of the ovarian activity in half of the females of G3 and 3/4 females of G2 was observed. It was detected the presence of corpora lutea in 1/4 of G3 transplants and half in G2. In follicular population all stages of development were observed. Comparing the receptor sites, it was observed a higher percentage of return to cyclicity, as well as of follicles, in animals which had the perimetrium as the site of transplantation. In the subcutaneous, there was a lower follicular growth and lower quantity of corpus luteum. Thus, ovarian autologous transplantation allocated in the subcutaneous tissue and perimetrium allowed both tissue maintenance and follicular development; however, the site of choice will depend on the final goal one aims with the technique


Objetivou-se comparar dois sítios receptores no transplante autólogo ovariano em camundongas. Doze camundongas BALB/c foram divididas aleatoriamente em três grupos experimentais com quatro animais em cada. O G1 - animais não submetidos ao transplante, G2 - animais que receberam o fragmento ovariano no perimétrio, G3 - animais que tiveram o tecido subcutâneo abdominal como sítio receptor. Para o transplante, as fêmeas foram submetidas à ovariossalpingectomia bilateral. O ovário esquerdo foi encaminhado para processamento histológico. O córtex do ovário direito foi fragmentado e reimplantado nos locais especificados de acordo com os grupos. Os transplantes foram recuperados em 3/4 das camundongas em cada grupo tratado. No lavado vaginal, observou-se em metade das fêmeas do G3 e em 3/4 das fêmeas do G2 retorno da ciclicidade. Observou-se presença de corpos lúteos em 1/4 dos transplantes do G3 e metade nos do G2. Na população folicular, foram observados todos os estádios de desenvolvimento. Comparando os sítios receptores observou-se maior porcentagem de retorno à ciclicidade, bem como de folículos, nos animais que tiveram o perimétrio como local do transplante. Já no subcutâneo houve menor crescimento folicular e menor quantidade de corpos lúteos. Dessa forma, conclui-se que o transplante autólogo ovariano alocado no tecido subcutâneo e no perimétrio permitiu tanto a manutenção tecidual quanto o desenvolvimento folicular, porém, o sítio de escolha vai depender do objetivo final que se almeja com a técnica


Assuntos
Feminino , Animais , Camundongos , Membrana Serosa/transplante , Ovário/transplante , Tela Subcutânea/transplante , Ovariectomia/veterinária , Salpingectomia/veterinária
11.
Acta Vet. bras. ; 10(1): 98-103, 2016. ilus, tab
Artigo em Português | VETINDEX | ID: vti-304289

RESUMO

The aim of this study was to compare two receptor sites in ovarian autologous transplantation in mice. Twelve Balb-c mice were randomly divided into three groups of four animals each: G1 - non-transplanted animals, G2 - animals which received the ovarian fragment in the perimetrium, G3 - animals which had the abdominal subcutaneous tissue as a receptor site. For transplantation, mice were subjected to bilateral salpingectomia. The left ovary was submitted for histological processing. The right ovarian cortex was fragmented and reimplanted in the specified places according to the groups. Transplants were recovered in 3/4 of the mice in each treatment group. In vaginal washes, the return of the ovarian activity in half of the females of G3 and 3/4 females of G2 was observed. It was detected the presence of corpora lutea in 1/4 of G3 transplants and half in G2. In follicular population all stages of development were observed. Comparing the receptor sites, it was observed a higher percentage of return to cyclicity, as well as of follicles, in animals which had the perimetrium as the site of transplantation. In the subcutaneous, there was a lower follicular growth and lower quantity of corpus luteum. Thus, ovarian autologous transplantation allocated in the subcutaneous tissue and perimetrium allowed both tissue maintenance and follicular development; however, the site of choice will depend on the final goal one aims with the technique(AU)


Objetivou-se comparar dois sítios receptores no transplante autólogo ovariano em camundongas. Doze camundongas BALB/c foram divididas aleatoriamente em três grupos experimentais com quatro animais em cada. O G1 - animais não submetidos ao transplante, G2 - animais que receberam o fragmento ovariano no perimétrio, G3 - animais que tiveram o tecido subcutâneo abdominal como sítio receptor. Para o transplante, as fêmeas foram submetidas à ovariossalpingectomia bilateral. O ovário esquerdo foi encaminhado para processamento histológico. O córtex do ovário direito foi fragmentado e reimplantado nos locais especificados de acordo com os grupos. Os transplantes foram recuperados em 3/4 das camundongas em cada grupo tratado. No lavado vaginal, observou-se em metade das fêmeas do G3 e em 3/4 das fêmeas do G2 retorno da ciclicidade. Observou-se presença de corpos lúteos em 1/4 dos transplantes do G3 e metade nos do G2. Na população folicular, foram observados todos os estádios de desenvolvimento. Comparando os sítios receptores observou-se maior porcentagem de retorno à ciclicidade, bem como de folículos, nos animais que tiveram o perimétrio como local do transplante. Já no subcutâneo houve menor crescimento folicular e menor quantidade de corpos lúteos. Dessa forma, conclui-se que o transplante autólogo ovariano alocado no tecido subcutâneo e no perimétrio permitiu tanto a manutenção tecidual quanto o desenvolvimento folicular, porém, o sítio de escolha vai depender do objetivo final que se almeja com a técnica(AU)


Assuntos
Animais , Feminino , Camundongos , Ovário/transplante , Tela Subcutânea/transplante , Membrana Serosa/transplante , Salpingectomia/veterinária , Ovariectomia/veterinária
18.
Acta Vet. Brasilica ; 1(1): 43-44, 2007. ilus
Artigo em Português | VETINDEX | ID: biblio-1453066

RESUMO

Os catetos (Tayassu tajacu) são animais silvestres que vêm sendo amplamente criados com finalidades comerciais e de conservação. No entanto, a ocorrência de malformações é um dos fatores que contribui para a redução na eficiência reprodutiva. A artrogripose é uma malformação caracterizada por curvatura ou constante flexão ou extensão do membro. O presente relato procura descrever o primeiro caso de artrogripose em um cateto. O caso ocorreu no Centro de Multiplicação de Animais Silvestres (CEMAS) da Universidade Federal Rural do Semi-árido (UFERSA), Mossoró, RN. Tratava-se de um filhote de cateto, macho, com 3 dias de idade, que foi encontrado fraco, pois não conseguia mamar em sua mãe. Foi verificada artrogripose em todos os membros, mas nenhuma outra alteração foi encontrada. A ocorrência da artrogripose foi capaz de afetar o filhote, que foi rejeitado pela mãe, o que acabou resultando em sua morte.


Collared peccaries are wild animals that have been bred for commercial and conservational purposes. However, the occurrence of malformations is one of the factors that contribute to reduction of reproductive efficiency. Arthrogryposis is a malformation characterized by fore or hind members curvature, constant flexure or extension. The present report describes for the first time arthrogryposis in a collared peccary. The case occurred at Centre for Wild Animals Multiplication from Universidade Federal Rural do Semi-árido (UFERSA), Mossoró city, RN, Brazil. A 3 days old male collared peccary pup was found weak because it was not able to suckle on his mother. It was verified arthrogryposis in all members, but any other malformation was evidenced. The occurrence of arthrogryposis affected pup's survival. It was rejected by his mother; this leaded to its death.


Assuntos
Animais , Artrogripose/veterinária , Artiodáctilos/anormalidades , Anormalidades Congênitas/veterinária
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA