RESUMO
PURPOSE: In recent years, minimal intervention procedures (MIPs) for treating dental caries in children have stood out as an innovative method. Nevertheless, the treatment decision should be based on scientific evidence, professional expertise, and parents' preference/acceptance. Evaluating the acceptance of MIPs by parents is an essential factor, but little information is available on what guides this preference. METHODS: This scoping review aims to synthesize the evidence on parents'/caregivers' acceptance of MIP for managing cavitated caries lesions in children. A search was performed in the PubMed, Cochrane Library, Lilacs and Google Scholar databases with no restriction on date or language. RESULTS: A total of 19 articles were selected (6 clinical trials, 1 longitudinal and 12 cross-sectional studies). The application of silver diamino fluoride (SDF) was the most commonly evaluated procedure (n = 17), followed by the atraumatic restorative technique (ART) and the Hall Technique (HT). The acceptance of MIPs ranged from 1.4% to 100%, and the application of SDF was better accepted in posterior teeth and in uncooperative children. ART had better aesthetic acceptance than HT. CONCLUSION: Application of SDF, Hall Technique and ART was well accepted by parents/caregivers. However, a gap remains in the literature regarding the acceptance of other procedures. Therefore, further studies in this area will contribute toward a better understanding of the opinion of parents/caregivers, and thus improve caries lesion management in children.
Assuntos
Cárie Dentária , Fluoretos , Compostos de Prata , Criança , Humanos , Cárie Dentária/tratamento farmacológico , Estudos Transversais , Dente Decíduo , Assistência Odontológica , Pais , Fluoretos Tópicos/uso terapêuticoRESUMO
In this study, we investigate the energetics of small aluminum clusters doped with a carbon atom using several computational methods, including diffusion quantum Monte Carlo, Hartree-Fock (HF), and density functional theory. We calculate the lowest energy structure, total ground-state energy, electron population distribution, binding energy, and dissociation energy as a function of the cluster size of the carbon-doped aluminum clusters compared with the undoped ones. The obtained results show that carbon doping enhances the stability of the clusters mainly due to the electrostatic and exchange interactions from the HF contribution gain. The calculations also indicate that the dissociation energy required to remove the doped carbon atom is much larger than that required to remove an aluminum atom from the doped clusters. In general, our results are consistent with available theoretical and experimental data.
Assuntos
Alumínio , Teoria Quântica , Alumínio/química , Método de Monte Carlo , Difusão , ElétronsRESUMO
We present all-electron quantum Monte Carlo simulations on the anionic, neutral, and cationic boron clusters BQn with up to 13 atoms (Q = -1, 0, +1 and n ≤ 13). Accurate total energies of these clusters are obtained and an excellent agreement is reached with available experimental results for adiabatic and vertical detachment energies. We also perform very accurate Hartree-Fock calculations in the complete-basis-set limit where electron correlation is absent. In combination with the FN-DMC and HF-CBS results, we quantify the correlation effects and present the first attempt for a systematic investigation on the electron correlation effects in boron clusters. The obtained results show that, in general, electron correlation may contribute significantly to both the atomic and electronic structures of the boron clusters, manifested in the quantities such as the average binding energies of the clusters, atomic dissociation energies, detachment energies, and ionization potentials. For instance, the calculations indicate that the electron correlation maintains the bound state of cationic cluster B2+ and it also contributes 99% of the detachment energy of the anionic cluster B5-.
RESUMO
BACKGROUND: Using sulfated polysaccharides (SP) in fish sperm freezing medium promotes cell maintenance. OBJECTIVE: To evaluate the effect of different SP concentrations, extracted from two seaweeds (Gracilaria domingensis and Ulva fasciata), as a supplement to the sperm freezing medium of Prochilodus brevis. MATERIALS AND METHODS: Five semen pools were diluted in a solution composed of 5% glucose, 10 % dimethyl sulfoxide (DMSO) and different SP concentrations (0, 0.5, 1.0, 1.5, 2.0, 2.5 or 3.0 mg/mL). The samples were cryopreserved and, after 7 days, rewarmed and analyzed for morphology, plasma membrane integrity, DNA integrity, mitochondrial activity and sperm kinetics [total motility, progressive motility, curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), and wobble (WOB)]. RESULTS: There was no interaction between seaweed and SP concentrations. Similar effects were observed with SP extracted from the two seaweeds, regardless of concentration. When comparing the SP concentrations, regardless of the seaweed, 1.0 mg/mL SP showed better results for VCL and VSL. For VAP and WOB, 1.0 mg/mL SP showed better results, but differed from 3.0 mg/mL. LIN followed the same pattern, but differed from SP at 2.5 and 3.0 mg/mL. For progressive motility, 1.0 mg/mL G. domingensis showed superior results compared to the control. For mitochondrial activity, G. domingensis was superior to U. fasciata, regardless of concentration. The lowest concentrations (0.5 and 1.0 mg/mL) showed the best results, regardless of the seaweed. However, the control was superior to all treatments tested. CONCLUSION: G. domingensis SP at the lowest concentrations might be a potential supplement to the P. brevis freezing medium. doi.org/10.54680/fr22210110412.
Assuntos
Caraciformes , Preservação da Fertilidade , Preservação do Sêmen , Animais , Masculino , Congelamento , Criopreservação/métodos , Sulfatos , Motilidade dos Espermatozoides , Preservação do Sêmen/veterinária , Preservação do Sêmen/métodos , Espermatozoides , GlucoseRESUMO
The ground-state structural transition in small lithium clusters Lin (n = 4 - 6) is analyzed based on the many-body expansion of the interaction energy using the total energy calculated by the fixed-node diffusion Monte Carlo (FN-DMC) simulations. The results show that the transition from 2D to 3D structure occurs through an intricate competition of attractive and repulsive interaction energies. As the structure dimensionality increases from 2D to 3D, the electron-correlation contribution to the interaction energy in the isomer of the ground-state structure is always the largest.
RESUMO
We investigate many-electron correlation effects in neutral and charged coinage-metal clusters Cun, Agn, and Aun (n = 1-4) via ab initio calculations using fixed-node diffusion Monte Carlo (FN-DMC) simulations, density functional theory (DFT), and the Hartree-Fock (HF) method. From very accurate FN-DMC total energies of the clusters and the HF results in the infinity large complete-basis-set limit, we obtain correlation energies in these strongly correlated many-electron clusters involving d orbitals. The obtained bond lengths of the clusters, atomic binding and dissociation energies, ionization potentials, and electron affinities are in satisfactory agreement with the available experiments. In the analysis, the electron correlation effects on these observable physical quantities are quantified by relative correlation contributions determined by the difference between the calculated FN-DMC and HF results. We show that the correlation contribution is not only significant for the quantities related to electronic structures of the coinage-metal clusters, such as electron affinity, but it is also essential for the stability of the atomic structures of these clusters. For example, the electron correlation contribution is responsible for more than 90% of the atomic binding energies of the small neutral copper clusters. We also demonstrate the orbital-occupation dependence of the correlation energy and electron pairing of the valence electrons in these coinage-metal clusters from the electron correlation-energy gain and spin-multiplicity change in the electron addition processes, which are reflected in their ionization potentials and electron affinities.
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Porcine respirovirus 1 (PRV1) is an emerging virus in pigs that has been previously described in the USA and China. There are no reports of its presence in the rest of the world. The objective of this study was to determine the occurrence of PRV1 in Chile and to determine its phylogeny. Thus, we collected samples (oral fluids, nasal swabs, and lungs) from a swine influenza A virus (IAV) surveillance program, most of which belonged to pigs with respiratory disease. The samples were analyzed by RT-PCR, and the viral sequencing was obtained using RNA whole-genome sequencing approach. Maximum likelihood phylogeny was constructed with the available references. Thirty-one of 164 samples (18.9 %) were RT-PCR positive for PRV1: 62.5 % oral fluids, 19.0 % nasal swabs, and 8.6 % lungs. All 6 farms in this study had at least one positive sample, with 6-40 % of positive results per farm, which suggests that PRV1 is disseminated in Chilean swine farms. Twenty-one of 31 (677%) PRV1-positive samples were also positive for IAV, so the role of PRV1 as secondary pathogen in respiratory disease needs to be further evaluated. Near to complete genome of two PRV1s were obtained from two farms. The phylogenies, in general, showed low bootstrap support, except the concatenated genome and the L gene trees which showed clustering of the Chilean PRV1 with Asian sequences, suggesting a close genetic relationship. This is the first report of PRV1 in the Southern Hemisphere. Further studies are necessary to determine the genetic diversity of this virus in Chile.
Assuntos
Doenças Transmissíveis Emergentes/veterinária , Genoma Viral , Infecções por Orthomyxoviridae/veterinária , Filogenia , Respirovirus/genética , Doenças dos Suínos/virologia , Animais , Chile , Doenças Transmissíveis Emergentes/virologia , Fazendas , Respirovirus/isolamento & purificação , Análise de Sequência de DNA , Suínos , Sequenciamento Completo do GenomaRESUMO
Diarrheagenic (DEC) and avian pathogenic Escherichia coli (APEC) are associated with intestinal and extra-intestinal infections (ExPEC), respectively. We aimed to analyze the antimicrobial susceptibility, gene encoding virulence factors associated to DEC and APEC, and phylogenetic classification in E. coli isolated from 320 samples of feed and ingredients. Antimicrobial susceptibility was performed using the disk diffusion method and Multiple Antibiotic Resistance (MAR) Index and Multi-Drug Resistance (MDR) were calculated. Phylogenetic classification was performed on samples harboring DEC and/or APEC virulence-associated genes. A total of 110 E. coli strains were isolated in 15% (49/320) of the evaluated inputs (n=13 vegetable meal; n=33 animal meal, n=3 feed). In general, the isolates showed the highest rates of antimicrobial resistance to sulfonamide and cefazolin and 18% (20/110) were multi-drug resistant. MAR index of feed samples was the highest (0.467). Six and five strains had APEC and DEC virulence-associated genes, respectively, and belonging to phylogenetic groups A and B1. These findings point to the need for strict microbiological control during the production process of these foods.(AU)
Escherichia coli diarreiogênicas (DEC) e patogênicas para aves (APEC) são associadas a infecções intestinais e extraintestinais (ExPEC), respectivamente. O objetivo do presente trabalho foi avaliar a sensibilidade antimicrobiana, a presença de genes que codificam os fatores de virulência relacionados à DEC e APEC, e a classificação filogenética em E. coli isoladas de 320 amostras de ração para frangos e ingredientes. A sensibilidade antimicrobiana foi determinada pelo método disco-difusão e calculou-se o índice de resistência múltipla aos antimicrobianos (IRMA) e a resistência a múltiplas drogas (MDR). Nas amostras que possuíam genes de virulência relacionados à DEC e/ou APEC, foi realizada a classificação filogenética. Foram isoladas 110 amostras de E. coli em 15% (49/320) dos insumos avaliados (n=13 farelos vegetais; n=33 farinhas de origem animal; n=3 rações). De forma geral, os isolados apresentaram as maiores frequências de resistência antimicrobiana à sulfonamida e à cefazolina e 18% (20/110) foram resistentes a múltiplas drogas. O IRMA das rações foi o mais alto (0,467). Os genes que codificam fatores de virulência associados à APEC e DEC foram detectados em seis e cinco isolados, respectivamente, pertencentes aos grupos filogenéticos A e B1. Os resultados demonstram a necessidade de rigoroso controle microbiológico durante o processo de produção desses alimentos.(AU)
Assuntos
Animais , Galinhas/virologia , Fatores de Virulência , Diarreia/veterinária , Escherichia coli/isolamento & purificação , Ração Animal/microbiologia , Resistência Microbiana a MedicamentosRESUMO
Diarrheagenic (DEC) and avian pathogenic Escherichia coli (APEC) are associated with intestinal and extra-intestinal infections (ExPEC), respectively. We aimed to analyze the antimicrobial susceptibility, gene encoding virulence factors associated to DEC and APEC, and phylogenetic classification in E. coli isolated from 320 samples of feed and ingredients. Antimicrobial susceptibility was performed using the disk diffusion method and Multiple Antibiotic Resistance (MAR) Index and Multi-Drug Resistance (MDR) were calculated. Phylogenetic classification was performed on samples harboring DEC and/or APEC virulence-associated genes. A total of 110 E. coli strains were isolated in 15% (49/320) of the evaluated inputs (n=13 vegetable meal; n=33 animal meal, n=3 feed). In general, the isolates showed the highest rates of antimicrobial resistance to sulfonamide and cefazolin and 18% (20/110) were multi-drug resistant. MAR index of feed samples was the highest (0.467). Six and five strains had APEC and DEC virulence-associated genes, respectively, and belonging to phylogenetic groups A and B1. These findings point to the need for strict microbiological control during the production process of these foods.(AU)
Escherichia coli diarreiogênicas (DEC) e patogênicas para aves (APEC) são associadas a infecções intestinais e extraintestinais (ExPEC), respectivamente. O objetivo do presente trabalho foi avaliar a sensibilidade antimicrobiana, a presença de genes que codificam os fatores de virulência relacionados à DEC e APEC, e a classificação filogenética em E. coli isoladas de 320 amostras de ração para frangos e ingredientes. A sensibilidade antimicrobiana foi determinada pelo método disco-difusão e calculou-se o índice de resistência múltipla aos antimicrobianos (IRMA) e a resistência a múltiplas drogas (MDR). Nas amostras que possuíam genes de virulência relacionados à DEC e/ou APEC, foi realizada a classificação filogenética. Foram isoladas 110 amostras de E. coli em 15% (49/320) dos insumos avaliados (n=13 farelos vegetais; n=33 farinhas de origem animal; n=3 rações). De forma geral, os isolados apresentaram as maiores frequências de resistência antimicrobiana à sulfonamida e à cefazolina e 18% (20/110) foram resistentes a múltiplas drogas. O IRMA das rações foi o mais alto (0,467). Os genes que codificam fatores de virulência associados à APEC e DEC foram detectados em seis e cinco isolados, respectivamente, pertencentes aos grupos filogenéticos A e B1. Os resultados demonstram a necessidade de rigoroso controle microbiológico durante o processo de produção desses alimentos.(AU)
Assuntos
Animais , Galinhas/virologia , Fatores de Virulência , Diarreia/veterinária , Escherichia coli/isolamento & purificação , Ração Animal/microbiologia , Resistência Microbiana a MedicamentosRESUMO
Using the fixed-node diffusion Monte Carlo (FN-DMC) method, we calculate the total energy of small lithium clusters Lin (n = 2-6) to obtain the many-body decomposition of the interaction energy of 2- up to 6-body interactions. The obtained many-body decomposition of the interaction energy shows an alternating series with even and odd terms of attractive and repulsive contributions, respectively. The two-body attractive interactions guarantee the stability of the Li2, Li3, and Li4 clusters. For larger clusters Li5 and Li6, the 4-body attractive interactions are required for their stabilization once the strength of the 3-body repulsive interactions overwhelms that of the 2-body attractive ones. With increasing the cluster size, the additive and nonadditive contributions to the interaction energy increase linearly in magnitude but with different slopes for the two-dimensional (2D) planar and three-dimensional (3D) cagelike clusters. The significant increment in nonadditive effects from the 4-atom to the 5-atom cluster has driven the structural transition from 2D to 3D. Combining the FN-DMC calculations with the Hartree-Fock many-body decomposition of the interaction energy, we extract the correlation effects, showing that an odd-even competition pattern in the many-body repulsive and attractive interactions is crucial for the stabilization of the clusters.
RESUMO
The valence electron binding energies and the aromaticity of neutral and charged small boron clusters with three and four atoms are investigated using a combination of the fixed-node diffusion quantum Monte Carlo (FN-DMC) method, the density functional theory, and the Hartree-Fock approximation. The obtained electron binding energies such as the adiabatic detachment energy, vertical detachment energy, adiabatic ionization potential, and the vertical ionization potential are in excellent agreement with available experimental measurements. Their decomposition into three physical components such as the electrostatic potential and exchange interaction, the relaxation energy, and the electronic correlation effects has allowed us to determine that the neutral boron clusters are stabilized by the electrostatic and exchange interactions, while the anionic ones are stabilized by the relaxation and correlation effects. The aromaticity is studied based on electronic structure principles descriptor and on the resonance energy. The FN-DMC results from the electronic structure principles of the energy, hardness, and eletrophilicity have supported the aromaticity of B 3 - , B 4 - , and B4 and partially supported the aromaticity of the clusters B3, B 3 + , and B 4 + . The obtained values for the resonance energy of the clusters B 3 - , B3, B 3 + , B4, B 4 + , and B 4 - are 55.1(7), 54.2(8), 33.9(7), 84(1), 67(1), and 58(1) kcal/mol, respectively. Therefore, the order of decreasing stability of the trimer is B 3 - > B 3 > B 3 + , while for the tetramer it is B 4 > B 4 + > B 4 - , which is in agreement with the results from the molecular orbital analysis.
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A novel equine arteritis virus (EAV) was isolated and sequenced from feral donkeys in Chile. Phylogenetic analysis indicates that the new virus and South African asinine strains diverged at least 100 years from equine EAV strains. The results indicate that asinine strains belonged to a different EAV genotype.
Assuntos
Infecções por Arterivirus/veterinária , Equartevirus/isolamento & purificação , Equidae , Animais , Infecções por Arterivirus/virologia , Chile , Equartevirus/classificação , Equartevirus/genética , Masculino , Filogenia , Reação em Cadeia da Polimerase/veterinária , Proteínas Virais/análiseRESUMO
Using the fixed-node diffusion quantum Monte Carlo method, we calculate the total energy of small cationic and neutral lithium clusters. We estimate the ionization potential, atomic binding energy, dissociation energy, and the second difference in energy. We present a critical analysis of the structural and electronic properties of the clusters. The bond lengths and binding and dissociation energies obtained from the calculations are in excellent agreement with the available experimental results. A comparative analysis of the dissociation energy and the second difference in energy indicates that the cationic clusters Li3+, Li5+, and Li7+ are the most stable ones. We have also studied the electron correlation effects in the lithium clusters. The cationic clusters of odd-number size are relatively more favored in terms of correlation energy than their neighbors of even-number size. In the range of cluster sizes under investigation, we find that the contribution of electron correlation to ionization potential is not larger than 28% of its total values, whereas it enhances significantly the dissociation energy of the clusters reaching up to 70% of its total values for the most stable ones.
RESUMO
Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x108 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and presence of lesions such as aerosaculitis (A), perihepatitis (Ph), pericarditis (Pc), peritonitis (Pt), onfalitis (O) and cellulitis (C) were registered daily for seven days. From the second to the seventh day there was a proportional decrease in the punctuation of the time of death (TD) for each day that the bird had survived. The pathogenicity index was calculated using the following formula: PI = (TD x 5) + A + Ph + Pc + Pt + O + C. The obtainment of the PI of each bacterial sample was achieved by calculating the rate of the ten inoculated birds. Based on the obtained results, it was possible to attribute the pathogenicity value for each strain, which enabled us to classify them in groups of low (27/200), intermediate (95/200) and high (78/200) pathogenicity. The utilization of standards like time of death and presence of septicemic lesions made it possible to determine the pathogenicity rate for each strain. Besides that, the proposed model has presented dramatic differences between the high, intermediate and low pathogenicity groups, which makes this mechanism useful for further classification of strains isolated in poultry farms.
Salmonella Enteritidis e Salmonella Typhimurium são responsáveis por imensos prejuízos econômicos ao setor avícola, podendo levar ao desenvolvimento de doença clínica e ao aumento da mortalidade em aves jovens. A patogenicidade de Salmonella é considerada complexa e multifatorial, necessitando de estudos que possam esclarecer a interação entre patógeno e hospedeiro. O presente trabalho teve por objetivo avaliar a virulência de 130 isolados de S. Enteritidis e 70 de S.Typhimurium, inoculadas em pintos de um dia de idade, por meio do estabelecimento de um índice de patogenicidade. Para cada cepa, foram utilizados 10 pintos comerciais da linhagem Cobb. As aves foram inoculadas com 200µL de uma solução contendo 2x108 UFC de S. Enteritidis ou S. Typhimurium, por via intraperitoneal. A mortalidade e a presença de lesões como aerossaculite (A), peri-hepatite (Ph), pericardite (Pc), peritonite (Pt), onfalite (O) e celulite (C) foram registradas diariamente durante sete dias. Do segundo ao sétimo dia, houve uma diminuição proporcional da pontuação no tempo de morte (TM) a cada dia em que o animal sobrevivia. O cálculo do índice de patogenicidade de cada pintinho inoculado (IP) obedeceu à seguinte fórmula: IP = (TMx5) + A + Ph + Pc + Pt + O + C. Para obtenção do IP de cada amostra, foi realizada a média do IP obtido com as 10 aves inoculadas. Com base nos resultados observados, foi possível atribuir um valor de patogenicidade a cada uma das cepas, permitindo classificá-las em grupos de baixa (27/200), intermediária (95/200) e alta patogenicidade (78/200). A utilização de critérios, como tempo de morte e presença de lesões septicêmicas, permitiu a determinação de um índice de patogenicidade para cada cepa. Além disso, o modelo proposto apresentou diferença significativa entre os grupos de alta, intermediária e baixa patogenicidade, permitindo, assim, a sua aplicação para classificação futura das cepas isoladas em granjas avícolas.
Assuntos
Animais , Aves Domésticas , Salmonella enteritidis/patogenicidade , Salmonella typhimurium/patogenicidade , Salmonelose Animal/patologia , Interações Hospedeiro-Patógeno , Fatores de VirulênciaRESUMO
Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x108 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and presence of lesions such as aerosaculitis (A), perihepatitis (Ph), pericarditis (Pc), peritonitis (Pt), onfalitis (O) and cellulitis (C) were registered daily for seven days. From the second to the seventh day there was a proportional decrease in the punctuation of the time of death (TD) for each day that the bird had survived. The pathogenicity index was calculated using the following formula: PI = (TD x 5) + A + Ph + Pc + Pt + O + C. The obtainment of the PI of each bacterial sample was achieved by calculating the rate of the ten inoculated birds. Based on the obtained results, it was possible to attribute the pathogenicity value for each strain, which enabled us to classify them in groups of low (27/200), intermediate (95/200) and high (78/200) pathogenicity. The utilization of standards like time of death and presence of septicemic lesions made it possible to determine the pathogenicity rate for each strain. Besides that, the proposed model has presented dramatic differences between the high, intermediate and low pathogenicity groups, which makes this mechanism useful for further classification of strains isolated in poultry farms.(AU)
Salmonella Enteritidis e Salmonella Typhimurium são responsáveis por imensos prejuízos econômicos ao setor avícola, podendo levar ao desenvolvimento de doença clínica e ao aumento da mortalidade em aves jovens. A patogenicidade de Salmonella é considerada complexa e multifatorial, necessitando de estudos que possam esclarecer a interação entre patógeno e hospedeiro. O presente trabalho teve por objetivo avaliar a virulência de 130 isolados de S. Enteritidis e 70 de S.Typhimurium, inoculadas em pintos de um dia de idade, por meio do estabelecimento de um índice de patogenicidade. Para cada cepa, foram utilizados 10 pintos comerciais da linhagem Cobb. As aves foram inoculadas com 200µL de uma solução contendo 2x108 UFC de S. Enteritidis ou S. Typhimurium, por via intraperitoneal. A mortalidade e a presença de lesões como aerossaculite (A), peri-hepatite (Ph), pericardite (Pc), peritonite (Pt), onfalite (O) e celulite (C) foram registradas diariamente durante sete dias. Do segundo ao sétimo dia, houve uma diminuição proporcional da pontuação no tempo de morte (TM) a cada dia em que o animal sobrevivia. O cálculo do índice de patogenicidade de cada pintinho inoculado (IP) obedeceu à seguinte fórmula: IP = (TMx5) + A + Ph + Pc + Pt + O + C. Para obtenção do IP de cada amostra, foi realizada a média do IP obtido com as 10 aves inoculadas. Com base nos resultados observados, foi possível atribuir um valor de patogenicidade a cada uma das cepas, permitindo classificá-las em grupos de baixa (27/200), intermediária (95/200) e alta patogenicidade (78/200). A utilização de critérios, como tempo de morte e presença de lesões septicêmicas, permitiu a determinação de um índice de patogenicidade para cada cepa. Além disso, o modelo proposto apresentou diferença significativa entre os grupos de alta, intermediária e baixa patogenicidade, permitindo, assim, a sua aplicação para classificação futura das cepas isoladas em granjas avícolas.(AU)
Assuntos
Animais , Salmonella enteritidis/patogenicidade , Salmonella typhimurium/patogenicidade , Salmonelose Animal/patologia , Aves Domésticas , Fatores de Virulência , Interações Hospedeiro-PatógenoRESUMO
An investigation of the energetics of small lithium clusters doped either with a hydrogen or with a fluorine atom as a function of the number of lithium atoms using fixed-node diffusion quantum Monte Carlo (DMC) simulation is reported. It is found that the binding energy (BE) for the doped clusters increases in absolute values leading to a more stable system than for the pure ones in excellent agreement with available experimental measurements. The BE increases for pure, remains almost constant for hydrogenated, and decreases rapidly toward the bulk lithium for the fluoride as a function of the number of lithium atoms in the clusters. The BE, dissociation energy as well as the second difference in energy display a pronounced odd-even oscillation with the number of lithium atoms. The electron correlation inverts the odd-even oscillation pattern for the doped in comparison with the pure clusters and has an impact of 29%-83% to the BE being higher in the pure cluster followed by the hydrogenated and then by the fluoride. The dissociation energy and the second difference in energy indicate that the doped cluster Li3 H is the most stable whereas among the pure ones the more stable are Li2 , Li4 , and Li6 . The electron correlation energy is crucial for the stabilization of Li3 H. © 2016 Wiley Periodicals, Inc.
RESUMO
Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p 0.05) among the origins of the isolates (p 0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening.
Assuntos
Animais , Cólera/veterinária , Fatores de Virulência/análise , Fatores de Virulência/isolamento & purificação , Pasteurella multocida , GalinhasRESUMO
Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p 0.05) among the origins of the isolates (p 0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening.(AU)
Assuntos
Animais , Cólera/veterinária , Fatores de Virulência/análise , Fatores de Virulência/isolamento & purificação , Pasteurella multocida , GalinhasRESUMO
Foot-and-mouth disease (FMD) vaccines are routinely used as effective control tools in large regions worldwide and to limit outbreaks during epidemics. Vaccine-induced protection in cattle has been largely correlated with the FMD virus (FMDV)-specific antibodies. Genetic control of cattle immune adaptive responses has been demonstrated only for peptide antigens derived from FMDV structural proteins. Here, we quantify the heterogeneity in the antibody response of cattle primo-vaccinated against FMD and study its association with the genetic background in Holstein and Jersey sires. A total of 377 FMDV-seronegative calves (122 and 255 calves from 16 and 15 Holstein and Jersey sires, respectively) were included in the study. Samples were taken the day prior to primo-vaccination and 45 days post-vaccination (dpv). Animals received commercial tetravalent FMD single emulsion oil vaccines formulated with inactivated FMDV. Total FMDV-specific antibody responses were studied against three viral strains included in the vaccine, and antibody titres were determined by liquid-phase blocking ELISA. Three linear hierarchical mixed regression models, one for each strain, were formulated to assess the heterogeneity in the immune responses to vaccination. The dependent variables were the antibody titres induced against each FMDV strain at 45 dpv, whereas sire's 'breed' was included as a fixed effect, 'sire' was included as a random effect, and 'farm' was considered as a hierarchical factor to account for lack of independence of within herd measurements. A significant association was found between anti-FMDV antibody responses and sire's breed, with lower immune responses found in the Jersey sires' offspring compared with those from Holstein sires. No significant intrabreed variation was detected. In addition, farm management practices were similar in this study, and results of the serological assays were shown to be repeatable. It therefore seems plausible that differences in the immune response may be expected in the event of a mass vaccination campaigns.
Assuntos
Anticorpos Antivirais/imunologia , Doenças dos Bovinos/prevenção & controle , Febre Aftosa/prevenção & controle , Vacinação , Vacinas Virais/imunologia , Adjuvantes Imunológicos/uso terapêutico , Animais , Bovinos , Ensaio de Imunoadsorção Enzimática/veterinária , Febre Aftosa/imunologia , Vírus da Febre Aftosa/imunologia , Imunidade Humoral/imunologia , Análise de RegressãoRESUMO
We developed a methodology to assess nuclear quantum effects in phase boundaries calculations that is based on the dynamical integration of Clausius-Clapeyron equation using path integral simulations. The technique employs non-equilibrium simulations that are very efficient. The approach was applied to the calculation of the melting line of Ne in an interval of pressures ranging from 1 to 3366 bar. Our results show a very good agreement with both experimental findings and results from previous calculations. The methodology can be applied to solid and liquid phases, without limitations regarding anharmonicities. The method allows the computation of coexistence lines for wide intervals of pressure and temperature using, in principle, a single simulation.