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Avian Pathol ; 47(5): 489-496, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29974790

RESUMO

Chicken infectious anaemia virus (CIAV) is a widely distributed immunosuppressive agent. SPF flocks and eggs used for vaccine production and diagnostics must be CIAV-free. Detection of CIAV infection in SPF flocks involves primarily serology or other invasive methods. In order to evaluate different types of samples for rapid detection of CIAV infection, a trial was conducted in serologically negative broiler breeder pullets vaccinated with a commercial live-attenuated CIAV vaccine. Controls and vaccinated groups were sampled before and after vaccination. Invasive and non-invasive samples were used for CIAV DNA detection by real-time PCR. Seroconversion occurred at 14 days post-inoculation (DPI) in the vaccinated group, whereas CIAV genome was detected by qPCR at 7 DPI in both invasive and non-invasive samples. Only invasive samples remained qPCR positive for CIAV DNA by 21 DPI despite seroconversion of the chickens.


Assuntos
Vírus da Anemia da Galinha/genética , Galinhas , Infecções por Circoviridae/veterinária , Doenças das Aves Domésticas/virologia , Animais , Infecções por Circoviridae/virologia , DNA Viral/genética , Feminino , Masculino , Reação em Cadeia da Polimerase em Tempo Real
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