RESUMO

The aim of this work was to investigate how production and freeze-drying conditions of Bifidobacterium animalis subsp. lactis INL1, a probiotic strain isolated from breast milk, affected its survival and resistance to simulated gastric digestion during storage in food matrices. The determination of the resistance of bifidobacteria to simulated gastric digestion was useful for unveiling differences in cell sensitivity to varying conditions during biomass production, freeze-drying and incorporation of the strain into food products. These findings show that bifidobacteria can become sensitive to technological variables (biomass production, freeze-drying and the food matrix) without this fact being evidenced by plate counts.

Assuntos

Bifidobacterium/metabolismo , Microbiologia de Alimentos/métodos , Liofilização/métodos , Bifidobacterium/isolamento & purificação , Reatores Biológicos , Concentração de Íons de Hidrogênio , Cinética , Microscopia Eletrônica de Varredura