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1.
Molecules ; 27(15)2022 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-35956837

RESUMO

Psychoneuroendocrinoimmunology is the area of study of the intimate relationship between immune, physical, emotional, and psychological aspects. This new way of studying the human body and its diseases was initiated in the last century's first decades. However, the molecules that participate in the communication between the immune, endocrine, and neurological systems are still being discovered. This paper aims to describe the development of psychoneuroendocrinoimmunology, its scopes, limitations in actual medicine, and the extent of melatonin within it.


Assuntos
Melatonina , Sistema Endócrino , Humanos
2.
Int. j. morphol ; 40(5): 1209-1218, 2022. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1405272

RESUMO

SUMMARY: Di-(2-ethylhexyl) phthalate (DEHP) is among the most common plasticizer additives that humans are in contact with daily. DEHP can be released from plastic and enter the human body, whereby it is metabolized and transformed into oxidative hydrophilic molecules. Clinical follow-ups in patients exposed to this phthalate and investigations in cultures of several cell types have provided information on its effects. For example, it is associated with inhibition of diploid human cell development and morphological changes in cultured germ cells. Although skeletal muscle represents around 50 % of the human body mass, knowledge about the effects of DEHP on this tissue is poor. Cultured skeletal muscle cells were exposed to DEHP (1 mM) for 13 days with the aim of exploring and evaluating some of the potential morphological effects. Three culture development parameters and nine cell characteristics were monitored during the bioassay. At 13 days, growth area, cell viability, and concentration of total proteins were lower in DEHP exposed than in control cells. Cell width and area, as well as the diameter of the nucleus and nucleolus, were greater in exposed cells than in control cells. These are interpreted as signs of cytotoxicity and suggest potential adverse effects on the development of skeletal muscle cells from DEHP exposure, as reported for other cell types.


RESUMEN: Diariamente los seres humanos tenemos contacto con aditivos plastificantes, el di-(2-etilhexil) ftalato (DEHP) se encuentra entre los más comunes. El DEHP puede liberarse del plástico e ingresar al cuerpo humano, donde es metabolizado y transformando en moléculas hidrofílicas oxidativas. Seguimientos en pacientes expuestos a este ftalato e investigaciones en cultivos de varios tipos celulares han aportado información sobre sus efectos. El DEHP es asociado con la inhibición del desarrollo de células humanas diploides y cambios morfológicos en células germinales en cultivo. Sin embargo, aún es poco lo que se sabe sobre los efectos en el músculo esquelético, a pesar de que este tejido representa alrededor del 50 % de la masa corporal del humano. Para explorar y evaluar algunos efectos morfológicos en células de músculo esquelético, cultivos primarios fueron expuestos a DEHP (1 mM) durante 13 días. Se dio seguimiento a tres parámetros de desarrollo del cultivo y nueve características celulares. Al término de 13 días de exposición, los valores del área de crecimiento, viabilidad celular y concentración de proteínas totales fueron inferiores con respecto a los cultivos control. Se observaron cambios morfométricos en las células expuestas. Particularmente, el ancho y área celular, así como los diámetros del núcleo y nucleolos, fueron mayores a los registros en las células control. Estos resultados se interpretan como signos de citotoxicidad y sugieren efectos potencialmente adversos en el desarrollo de las células del músculo esquelético ante una exposición al DEHP, como se ha registrado para otros tipos celulares.


Assuntos
Humanos , Plastificantes/toxicidade , Músculo Esquelético/efeitos dos fármacos , Dietilexilftalato/toxicidade , Bioensaio , Músculo Esquelético/citologia , Poluentes Ambientais , Cultura Primária de Células
3.
Microorganisms ; 9(8)2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34442713

RESUMO

The intestinal epithelium serves as an effective barrier against the external environment, hampering the passage of potentially harmful substances (such as pathogenic microbes) that could trigger an exacerbated host immune response. The integrity of this barrier is thus essential for the maintenance of proper intestinal homeostasis and efficient protective reactions against chemical and microbial challenges. The principal consequence of intestinal barrier defects is an increase in intestinal permeability, which leads to an increased influx of luminal stressors, such as pathogens, toxins, and allergens, which in turn trigger inflammation and immune response. The fine and fragile balance of intestinal homeostasis can be altered by multiple factors that regulate barrier function, many of which are poorly understood. This review will address the role of gut microbiota as well as food supplements (such as probiotics, prebiotics, and synbiotics) in modulating gut health and regulating intestinal barrier function. In particular, we will focus on three human pathologies: inflammatory bowel disease, irritable bowel syndrome, and food allergy.

4.
Int J Neurosci ; 131(12): 1221-1230, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32571126

RESUMO

Efficient communication between the glial cells and neurons is a bi-directional process that is essential for conserving normal functioning in the central nervous system (CNS). Neurons dynamically regulate other brain cells in the healthy brain, yet little is known about the first pathways involving oligodendrocytes and neurons. Oligodendrocytes are the myelin-forming cells in the CNS that are needed for the propagation of action potentials along axons and additionally serve to support neurons by neurotrophic factors (NFTs). In demyelinating diseases, like multiple sclerosis (MS), oligodendrocytes are thought to be the victims. Axonal damage begins early and remains silent for years, and neurological disability develops when a threshold of axonal loss is reached, and the compensatory mechanisms are depleted. Three hypotheses have been proposed to explain axonal damage: 1) the damage is caused by an inflammatory process; 2) there is an excessive accumulation of intra-axonal calcium levels; and, 3) demyelinated axons evolve to a degenerative process resulting from the lack of trophic support provided by myelin or myelin-forming cells. Although MS was traditionally considered to be a white matter disease, the demyelination process also occurs in the cerebral cortex. Recent data supports the notion that initial response is triggered by CNS injury. Thus, the understanding of the role of neuron-glial neurophysiology would help provide us with further explanations. We should take in account the suggestion that MS is in part an autoimmune disease that involves genetic and environmental factors, and the pathological response leads to demyelination, axonal loss and inflammatory infiltrates.


Assuntos
Fenômenos Eletrofisiológicos/fisiologia , Imunidade/fisiologia , Esclerose Múltipla/imunologia , Esclerose Múltipla/fisiopatologia , Oligodendroglia/fisiologia , Animais , Fenômenos Eletrofisiológicos/imunologia , Humanos , Imunidade/imunologia , Esclerose Múltipla/metabolismo , Esclerose Múltipla/patologia , Oligodendroglia/imunologia , Oligodendroglia/metabolismo , Oligodendroglia/patologia
5.
Invest. clín ; Invest. clín;56(2): 201-214, jun. 2015. ilus, graf
Artigo em Espanhol | LILACS | ID: biblio-841079

RESUMO

La esclerosis múltiple (EM) es la principal causa de discapacidad neurológica de origen no traumático en adultos jóvenes. EM es una enfermedad crónica inflamatoria que se caracteriza por daño a las fibras nerviosas y la cubierta de mielina. Esto produce una gran variedad de síntomas una vez que nervios específicos muestran inflamación y pérdida de su función. Estudios epidemiológicos y experimentales han identificado alteraciones genéticas, anormalidades en enzimas antioxidantes y autoinmunidad como algunos de los factores de riesgo para el desarrollo de la enfermedad. Evidencia reciente sugiere que la inflamación y el estrés oxidativo en el sistema nervioso central contribuyen al daño del tejido cerebral. Las células residentes en el sistema nervioso central así como las células inflamatorias invasivas liberan una gran cantidad de especies reactivas de oxígeno y nitrógeno, las cuales causan desmielinización y destrucción de los axones: los hallazgos histopatológicos de la esclerosis múltiple. La interacción entre los procesos inflamatorios y neurodegenerativos producen perturbaciones neurológicas intermitentes seguidas por la acumulación progresiva de la discapacidad. Para tratar de limitar o disminuir la progresión de la enfermedad es necesario reducir la inflamación y el estrés oxidativo como estrategia terapéutica importante. Con la finalidad de mejorar la sobrevivencia y la calidad de vida de los pacientes, se están desarrollando ensayos clínicos con suplementos alimenticios tales como los antioxidantes y los ácidos grasos poliinsaturados omega-3.


Multiple sclerosis is the most common cause of progressive neurological disability in young adults. This disease involves damage to the myelin sheath that normally insulates the electrical activity of nerve fibers. This leads to a wide range of symptoms as specific nerves become injured and lose their function. Epidemiological and experimental studies show that genetic alterations, antioxidant enzyme abnormalities and autoimmunity are risk factors for developing the disease. Recent evidence suggests that inflammation and oxidative stress within the central nervous system are major causes of ongoing tissue damage. Resident central nervous system cells and invading inflammatory cells release several reactive oxygen and nitrogen species which cause the histopathological features of multiple sclerosis: demyelization and axonal damage. The interplay between inflammatory and neurodegenerative processes results in an intermittent neurological disturbance followed by progressive accumulation of disability. Reductions in inflammation and oxidative stress status are important therapeutic strategies to slow or halt the disease processes. Therefore, several drugs are currently in trial in clinical practice to target this mechanism; particularly the use of supplements such as antioxidants and omega-3 polyunsaturated fatty acids, in order to improve the survival and quality of patients’ lives.


Assuntos
Adulto , Humanos , Adulto Jovem , Desenho de Fármacos , Esclerose Múltipla/fisiopatologia , Neurônios/patologia , Qualidade de Vida , Axônios/patologia , Espécies Reativas de Oxigênio/metabolismo , Estresse Oxidativo/fisiologia , Inflamação/fisiopatologia , Esclerose Múltipla/tratamento farmacológico , Antioxidantes/metabolismo
6.
Clin Dev Immunol ; 2013: 708659, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24174971

RESUMO

Multiple sclerosis (MS) exhibits many of the hallmarks of an inflammatory autoimmune disorder including breakdown of the blood-brain barrier (BBB), the recruitment of lymphocytes, microglia, and macrophages to lesion sites, the presence of multiple lesions, generally being more pronounced in the brain stem and spinal cord, the predominantly perivascular location of lesions, the temporal maturation of lesions from inflammation through demyelination, to gliosis and partial remyelination, and the presence of immunoglobulin in the central nervous system and cerebrospinal fluid. Lymphocytes activated in the periphery infiltrate the central nervous system to trigger a local immune response that ultimately damages myelin and axons. Pro-inflammatory cytokines amplify the inflammatory cascade by compromising the BBB, recruiting immune cells from the periphery, and activating resident microglia. inflammation-associated oxidative burst in activated microglia and macrophages plays an important role in the demyelination and free radical-mediated tissue injury in the pathogenesis of MS. The inflammatory environment in demyelinating lesions leads to the generation of oxygen- and nitrogen-free radicals as well as proinflammatory cytokines which contribute to the development and progression of the disease. Inflammation can lead to oxidative stress and vice versa. Thus, oxidative stress and inflammation are involved in a self-perpetuating cycle.


Assuntos
Esclerose Múltipla/imunologia , Esclerose Múltipla/metabolismo , Estresse Oxidativo , Animais , Antioxidantes/metabolismo , Citocinas/metabolismo , Humanos , Peroxidação de Lipídeos , NF-kappa B/metabolismo
7.
Environ Mol Mutagen ; 41(1): 64-8, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-12552593

RESUMO

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a neurotoxin that induces Parkinsonism in humans, monkeys, and mice and oxidative stress in mammalian cells and tissues. In the present study, the relationship between the generation of lipid peroxidation products and DNA damage was studied in mice treated with MPTP. The frequency of micronucleated polychromatic erythrocytes (MN-PCE) and the concentrations of malonaldehyde and 4-hydroxyalkenals were determined in the bone marrow and peripheral blood of mice 0, 24, 48, 72, and 96 hr after treatment with MPTP, cyclophosphamide as a positive control, or diluent. Both MN-PCE and the lipid peroxidation products increased in MPTP-treated mice, with significant levels being detected in bone marrow starting at 24 hr after treatment and in blood starting at 48 hr after treatment. These results suggest that the generation of oxidative products is related to the DNA damage produced by MPTP in mice.


Assuntos
1-Metil-4-Fenil-1,2,3,6-Tetra-Hidropiridina/efeitos adversos , Sangue/efeitos dos fármacos , Medula Óssea/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Dopaminérgicos/efeitos adversos , Peroxidação de Lipídeos/efeitos dos fármacos , Animais , Eritrócitos/efeitos dos fármacos , Eritrócitos/fisiologia , Intoxicação por MPTP/genética , Intoxicação por MPTP/metabolismo , Masculino , Malondialdeído/análise , Camundongos , Camundongos Endogâmicos C57BL , Testes para Micronúcleos , Estresse Oxidativo
8.
Arch. med. res ; Arch. med. res;30(2): 125-7, mar.-abr. 1999. ilus
Artigo em Inglês | LILACS | ID: lil-256635

RESUMO

Background. The noncompetitive NMDA antagonists phencyclidine (PCP) and dizocilpine (MK-801) have been considered for use as neuroprotective therapeutic agents, although both produce injury in neurons of cingulate and retrosplenial cortices in rodents. The low-affinity, noncompetitive NMDA antagonist dextrorphan has been considered for use as a neuroprotective therapeutic drug. The aim of the present work was to evaluate the neurotoxicity of dextrorphan. Methods. Sprague-Dawley male rats were used and injected with either saline or dextorphan (30 mg/kg i.p.). The animals were sacrificed 30 min later, and the brain was examined for histopathological changes. Results. After. systemic administration of the drug, hyperchromatic and shrunken nuclei with chromatin condensation and disruption were observed. Also. granular and vacuolated cytoplasm was apparent in pyramidal neurons in the retrosplenial (posterior cingulate) cortex. Status spongious (spongy degeneration) of the neuropil was also detected. Conclusions. Morphological changes are similar to those described previously, which are induced by high-affinity, noncompetitive NMDA antagonists, such as MK-801


Assuntos
Animais , Masculino , Ratos , Dextrorfano/efeitos adversos , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/efeitos adversos , Cérebro/efeitos dos fármacos , Ratos Sprague-Dawley
9.
Arch. neurociencias ; Arch. neurociencias;2(4): 274-81, oct.-dic. 1997. tab, ilus
Artigo em Espanhol | LILACS | ID: lil-227208

RESUMO

La glándula de Harder es una glándula túbulo-alveolar localizada en la parte posterior de la órbita ocular de animales que poseen membrana nictitante. En estos mamiferos la glándula contiene una gran cantidad de lípidos. La glándula de Harder de roedores contiene un pigmento café rojizo, el cual ha sido identificado como porfirina. Las funciones de la glándula de Harder son; síntesis y liberación de ferhormonas, fotoprotección y termorregulación, osmoprotección y se le ha propuesto además un papel inmunoendocrino


Assuntos
Glândula de Harder/fisiologia , Glândula de Harder/imunologia , Transdutores/classificação , Transdutores/veterinária , Neuroimunomodulação/fisiologia , Feromônios/metabolismo , Porfirinas/metabolismo , Regulação da Temperatura Corporal/imunologia
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