RESUMO
Resistance to organophosphorus insecticides (malathion, chlorpyriphos, pyrimiphos-methyl); carbamates (propoxur); and pyrethroids (permethrin, deltametrine, cypermetrine, and lambda cialotrine) was studied in field populations of Musca domestica; results were compared with a susceptible reference strain. The method of topic application was employed in the bioassays; the synergist effect of the s,s,s tributyl phosphorotrioate and of the piperonyl butoxide (PB) was also tested. Biochemical microplate tests were also carried out to determine the presence of esterase and acetylcholinesterase enzymes in the three populations. Generalized resistance to malathion (FR = 264.6; 164.0; 154.7) was observed.
Assuntos
Carbamatos , Moscas Domésticas , Resistência a Inseticidas , Inseticidas , Compostos Organofosforados , Plantas , Piretrinas , AnimaisRESUMO
The application of a rapid technique to determine the frequency of the altered acetylcholinesterase gene which confers cross resistance to organophosphate and carbamate insecticides to mosquitoes, was tested. Laboratory strains of Culex quinquefasciatus Say, 1823 were used to apply this technique: A clear differentiation in absorbance values was attained among resistant susceptible homozygous mosquitoes (SS), heterozygous (RS); and homozygous (RR), with residual enzymatic activity values of 10-60; 70-100; and 80-120, respectively. This was clearly differentiated at first sight due to a change of the reaction's yellow color. The gene of the altered acetylcholinesterase was found in the Culex quinquefasciatus strains under study.
Assuntos
Acetilcolinesterase/análise , Carbamatos , Culex/enzimologia , Insetos Vetores/enzimologia , Inseticidas/antagonistas & inibidores , Compostos Organofosforados , Acetilcolinesterase/genética , Animais , Cuba , Culex/genética , Frequência do Gene , Heterozigoto , Homozigoto , Insetos Vetores/genética , Resistência a Inseticidas/genética , MétodosRESUMO
Three methods of collecting adult anophelines were comparatively studied in a rural town in Havana Province, during the period comprised between June and September 1986. The methods of capture used were as follows: in resting sites, in human bait and through lactic acid CDC light traps. A greater number of anophelines (F = 6.86; p less than 0.01) was collected on human bait. The capture of 11 species of culicines was reported.