RESUMO
The present study aims to elucidate in a sequential manner the changes of the blood coagulation process at different phases of experimental schistosomiasis, comprising the pre-patent, acute, intermediate and chronic phases, and the effect of chemotherapeutic cure, at the acute and chronic phases, on reversion of changes related to the coagulation factors. Mice were infected with Schistosoma mansoni cercariae, and were divided into four groups. Blood samples from these groups were collected 32, 70, 100, and 140 days after infection, corresponding to the pre-patent, acute, intermediate and chronic phases, respectively. Simultaneously, other infected groups were given oxamniquine, 70 and 140 days after infection. At the same time as blood collection from infected and/or treated animal groups, other uninfected control animal groups were punctured and maintained under the same conditions as the infected animals. The vitamin-K-dependent clotting factors were found to be more sensitive to infection at different phases, while factors VIII and XI presented hyperactivity. Results obtained 90 days after chemotherapeutic treatment with oxamniquine, administered at the acute and chronic phases, presented noticeable reversion of the main alterations in the coagulation mechanism. The present study provides unquestionable data on the development of hemostatic changes throughout the course of S. mansoni infection.
Assuntos
Oxamniquine/uso terapêutico , Esquistossomose mansoni/sangue , Esquistossomose mansoni/tratamento farmacológico , Esquistossomicidas/uso terapêutico , Doença Aguda , Animais , Coagulação Sanguínea/efeitos dos fármacos , Coagulação Sanguínea/fisiologia , Fatores de Coagulação Sanguínea/metabolismo , Doença Crônica , Feminino , Camundongos , Schistosoma mansoni , Esquistossomose mansoni/parasitologiaRESUMO
Cercariae of Schistosoma mansoni after intraperitoneal inoculation, developed and reached the sexual maturation in that site with egg production. In the AKR/J strain of mice, 7.7 of the peritoneal recovered females showed normal eggs in the uterus. No evidence of hemoglobinic pigment in their digestive tract was observed in the peritoneal recovered parasites from both strains (AKR/J and SWISS). This fact suggests that the parasites can develop without red blood cells ingestion. On the other hand, the development of the parasite with egg production in the peritoneal cavity of the AKR/J mouse reinforces the data that the lung phase is not necessary for the development of the parasite.