RESUMO
The lymphoproliferative responses of 51 leprosy patients and 11 healthy contacts were analyzed using the nitrocellulose-bound specific antigen fractions from the cell-free extract of Mycobacterium leprae. The main proliferation-inducing fraction for peripheral blood mononuclear cells of the healthy contacts was found to be the Fraction II, bearing antigens in the range of 66-45 kDa. However, this fraction failed to induce lymphoproliferation in the leprosy patients, unlike healthy contacts (p < 0.032). The number of responders as well as the strength of the responses to 66-45 kDa proteins were found to be low in the leprosy patients compared to the healthy contacts. Further, preliminary analysis with the subfractions of Fraction II produced a similar pattern, suggesting that the immune response to the antigens in the range of 66-45 kDa M. leprae proteins remains suppressed in subjects with clinical signs and symptoms of the disease.
Assuntos
Colódio , Colódio/análise , Hanseníase/genética , Hanseníase/imunologia , Mycobacterium leprae/genética , Mycobacterium leprae/imunologiaAssuntos
Alelos , Hanseníase Dimorfa/genética , Hanseníase Dimorfa/imunologia , Hanseníase Tuberculoide/genética , Hanseníase Tuberculoide/imunologia , Hanseníase Virchowiana , Hanseníase/genética , Hanseníase/imunologia , Heterozigoto , Homozigoto , Linfócitos T/imunologia , Polimorfismo Genético , Rearranjo Gênico do Linfócito T , Receptores de Antígenos de Linfócitos T alfa-beta/genéticaAssuntos
Alelos , Antígenos de Bactérias/genética , Antígenos de Diferenciação/genética , Ativação Linfocitária , /genética , Dados de Sequência Molecular , Fenótipo , Hanseníase Dimorfa/genética , Hanseníase Dimorfa/imunologia , Hanseníase Tuberculoide/genética , Hanseníase Tuberculoide/imunologia , Hanseníase Virchowiana/genética , Hanseníase Virchowiana/imunologia , Hanseníase/genética , Hanseníase/imunologia , Homozigoto , Imunidade Celular , Mycobacterium leprae/imunologia , Polimorfismo Genético , Pró-Colágeno/genética , Reação em Cadeia da PolimeraseRESUMO
Cryostat sections of dermal lesions from 30 untreated leprosy patients were studied by indirect immunofluorescence using monoclonal antibodies defining T cell subsets and Ia like antigens. Most lymphocytes in leprosy lesions were positive for OKT3 and Ia like antigens indicating thereby the presence of activated T cells. Maximal numbers of these cells were seen in localized paucibacillary tuberculoid leprosy lesions in close association with epithelioid cells. A decline in their numbers was observed over the leprosy spectrum with a marked reduction in disseminated, multi-bacillary, lepromatous leprosy where only scattered OKT3+ cells were visualized. OKT4 and OKT8 positive cells defining T cell subsets, were frequently found within the OKT3+ lymphocytes throughout the leprosy spectrum. The ratio of OKT4+/OKT8+ cells ranged from 1.2 to 5.0 in tuberculoid and from 0.2 to 1.0 in lepromatous lesions. Macrophages in the granulomas stained intensely with anti-Ia antisera. Ia like antigens were expressed to the same degree on macrophages with or without intracellular acid fast bacilli.
Assuntos
Humanos , Contagem de Leucócitos , Granuloma/imunologia , Granuloma/patologia , Hanseníase/imunologia , Hanseníase/patologia , Linfócitos T/classificação , Linfócitos T/imunologia , Pele/imunologia , Pele/patologiaRESUMO
Antigens prepared from five mycobacteria viz. M. leprae M. vaccae, M. phlei, M.W and ICRC bacillus were used for assessing cross reactivity between different antigens on sixteen patients with different forms of leprosy. ICRC bacillus and M.W gave the best correlation with M. leprae.
Assuntos
Humanos , Antígeno de Mitsuda/imunologia , Antígenos de Bactérias/imunologia , Hanseníase/imunologia , Hanseníase/microbiologia , Hipersensibilidade Tardia/diagnóstico , Mycobacterium leprae/imunologia , Mycobacterium phlei/imunologia , Mycobacterium/imunologiaRESUMO
A reduction in number of a subpopulation of T lymphocytes was noted in lepromatous leprosy cases with high bacillary load. Tuberculoid and treated lepromatous patients, who were bacillary negative had normal levels of these cells. B-cell numbers were high in lepromatous patients of treatment and bacillary load.