RESUMO
With the rapid growth of nanotechnology and the applications of nanoparticles, environmental exposure to these particles is increasing. However, their impact in human and environmental health is not well studied. Anurans, with life stage comprising embryos, tadpoles and adults, have an extremely permeable skin which makes them excellent indicators of environmental health. This study evaluated the acute toxicity effects of polyaniline nanoparticles (PANI-Np) in different dispersant on embryos and larvae of Rhinella arenarum. The results showed that LC50 of PANI-Np dispersed in polyvinylpyrrolidone (PVP) were 1,500 mg/L, while LC50 by PANI-Np dispersed in PVP+PNIPAM (polyN-isopropylacrilamide) showed a highest toxicity (1,170 mg/L). The embryo teratogenicity increased with increasing exposure concentration in both kinds of PANI-Np although in PANI-Np1, there is an increased teratogenic effect associated with the polymer stabilizer PVP.
Assuntos
Compostos de Anilina/toxicidade , Nanopartículas/toxicidade , Teratogênicos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Bufo arenarum , Embrião não Mamífero/efeitos dos fármacos , Larva/efeitos dos fármacos , Modelos AnimaisRESUMO
The fate and effect of nanomaterials in the environment is of paramount importance towards the technological application of the materials. This work shows the ecotoxicological potential of polyaniline (PANI) nanofibers in the larvae Rhinella arenarum by means of AMPHITOX test. Acute toxicity of PANI nanofibers towards embryos of the common South American toad R. arenarum (Anura: bufonidae) was evaluated in the premetamorphosis (stage 25) larvae. The exposure of R. arenarum larvae to at dose of 150, 250 and 400 mg L(-1) resulted in 100% viability within 96 h exposure. The embryos at 2-4 blastomers stage (early life stage teratogenic test) revealed that embryos were not killed and no teratogenic effects were observed when embryos were incubated with PANI nanofibers (150 and 250 mg L(-1)), while only a growth retardation of embryos was induced at levels of 250 mg PANI nanofibers L(-1). On the other hand, at 400 mg L(-1) concentration, a reduction in the body length of larvae and tail malformation was observed. This results suggest that a concentration-dependent toxicity is operative, typified by phenotypes that had abnormal body axes. The presence of PANI nanofibers in gut contents and its excretion by larval stages of R. arenarum was confirmed by UV-visible spectroscopy.