RESUMO
Se analizaron 41 cepas de Escherichia coli, aisladas en niños menores de 5 años con diarreas agudas, procedentes de las diferentes provincias del país. Fueron probados 4 importantes determinantes fenotípicos: sorbosa, sorbitol, enterohemolisina y la serología de O157: H7, para seleccionar las cepas de la categoría enterohemorrágica o productora de toxina shiga. De igual modo fueron caracterizadas por el método de biotipaje y de susceptibilidad antimicrobiana. El uso de las pruebas fenotípicas mostró 6 cepas con características presuntivas, de estas, 4 mostraron mayor probabilidad de pertenecer a la categoría productora de toxina shiga. En el ensayo de susceptibilidad antimicrobiana las cepas mostraron elevada resistencia, sobre todo para la ampicilina y el trimetropin-sulfametoxasol. Otro hallazgo de interés fueron los valores de resistencia y de susceptibilidad intermedia obtenidos para el augmentin, aztreonan y ceftriaxona. Se alcanzaron 12 patrones de resistencia antimicrobiana y de ellos 10 resaltaron por ser multirresistentes(AU)
Forty strains of Escherichia coli isolated from children under 5 years of age with acute diarreas, coming from different provinces of the country , were analyzed. Four important phenotypical determinants were tested: sorbosa, sorbitol, enterohemolysin and 0157: H7 serology, in order to select those strains from enterohemorrhagic or Shiga toxin-producing category. Likewise, they were characterized by biotyping and antimicrobial susceptibility methods. The use of phenotypical tests showed six strains with presumptive characteristics, four of which were most likely to be Shiga toxin-producing strains. In antimicrobial susceptibility test, the strains showed high resistance mainly to ampicillin and trimethrophin-sulfamethoxasole. Another interesting finding were intermediate resistance and susceptibility values to augmentin, aztreonan and ceftriaxone. There were 12 antimicrobial resistance patterns of which 10 were multi-resistant(AU)
Assuntos
Escherichia coli/patogenicidade , Produtos com Ação AntimicrobianaRESUMO
Se analizaron 41 cepas de Escherichia coli, aisladas en niños menores de 5 años con diarreas agudas, procedentes de las diferentes provincias del país. Fueron probados 4 importantes determinantes fenotípicos: sorbosa, sorbitol, enterohemolisina y la serología de O157: H7, para seleccionar las cepas de la categoría enterohemorrágica o productora de toxina shiga. De igual modo fueron caracterizadas por el método de biotipaje y de susceptibilidad antimicrobiana. El uso de las pruebas fenotípicas mostró 6 cepas con características presuntivas, de estas, 4 mostraron mayor probabilidad de pertenecer a la categoría productora de toxina shiga. En el ensayo de susceptibilidad antimicrobiana las cepas mostraron elevada resistencia, sobre todo para la ampicilina y el trimetropin-sulfametoxasol. Otro hallazgo de interés fueron los valores de resistencia y de susceptibilidad intermedia obtenidos para el augmentin, aztreonan y ceftriaxona. Se alcanzaron 12 patrones de resistencia antimicrobiana y de ellos 10 resaltaron por ser multirresistentes.
Forty strains of Escherichia coli isolated from children under 5 years of age with acute diarreas, coming from different provinces of the country , were analyzed. Four important phenotypical determinants were tested: sorbosa, sorbitol, enterohemolysin and 0157: H7 serology, in order to select those strains from enterohemorrhagic or Shiga toxin-producing category. Likewise, they were characterized by biotyping and antimicrobial susceptibility methods. The use of phenotypical tests showed six strains with presumptive characteristics, four of which were most likely to be Shiga toxin-producing strains. In antimicrobial susceptibility test, the strains showed high resistance mainly to ampicillin and trimethrophin-sulfamethoxasole. Another interesting finding were intermediate resistance and susceptibility values to augmentin, aztreonan and ceftriaxone. There were 12 antimicrobial resistance patterns of which 10 were multi-resistant.
Assuntos
Escherichia coli/patogenicidade , Produtos com Ação AntimicrobianaRESUMO
Forty strains of Escherichia coli isolated from children under 5 years of age with acute diarreas, coming from different provinces of the country , were analyzed. Four important phenotypical determinants were tested: sorbosa, sorbitol, enterohemolysin and 0157:H7 serology, in order to select those strains from enterohemorrhagic or Shiga toxin-producing category. Likewise, they were characterized by biotyping and antimicrobial susceptibility methods. The use of phenotypical tests showed six strains with presumptive characteristics, four of which were most likely to be Shiga toxin-producing strains. In antimicrobial susceptibility test, the strains showed high resistance mainly to ampicillin and trimethrophin-sulfamethoxasole. Another interesting finding were intermediate resistance and susceptibility values to augmentin, aztreonan and ceftriaxone. There were 12 antimicrobial resistance patterns of which 10 were multi-resistant.