RESUMO

Fasciola hepatica, the liver trematode, infects ruminants and causes economic loss. Because parasites are developing resistance to commercial drugs, the negative effects of parasitism are increasing. In this study, we aimed to evaluate the efficacy of cumin (Cuminum cyminum) essential oil against F. hepatica eggs and adults. The eggs were incubated with eight concentrations of the essential oil (0.031125-4.15 mg/mL), and viable eggs were counted after 14 days and classified as embryonated or non-embryonated. Adult flukes were incubated in Roswell Park Memorial Institute medium to ensure their viability and then incubated in essential oil. They were observed for 24 h after treatment. The adults were assessed with the two lowest effective oil concentrations used in the ovicidal test. Three controls were used for both tests: nitroxynil, a negative control, and Tween®80. After incubation in oil, the adult specimens were processed for histological analysis and stained with hematoxylin-eosin. In addition, the oil was tested for cytotoxicity using Madin-Darby bovine kidney cells to assess any possible effect on them. The oil was effective in ovicidal and adulticidal inhibition of the trematode, with statistically significant results. All concentrations assessed in the ovicidal test were 100% effective. The adult test was effective within 15 h and inactivated all the specimens at the highest concentration evaluated (0.06225 mg/mL). Histological analysis showed that cumin essential oil resulted in marked areas of vacuolization. The spines showed no structural changes but were surrounded by microvesicles. These findings indicated that cumin oil could be a potential compound in the control of fasciolosis.

Assuntos

RESUMO

OBJECTIVE: This study was done to evaluate the in vitro and in vivo effects of the essential oil (OE-CL) and nanoemulsion (N-CL) of Cymbopogon flexuosus against Trichomonas gallinae. MATERIALS AND METHODS: In vitro assays were done with 106 parasites and OE-CL and N-CL in the concentrations: 110, 220, 330, 440, 550, 660, 770 and 880 µg/ml and four controls: CN (culture medium and trophozoites), MTZ (trophozoites plus 800 µg/ml of metronidazole), TW (trophozoites plus vehicles used for solubilization of derivatives (0.01% Tween) and NB (blank nanoemulsion 880 µg/ml). The in vivo assay was done in 35 quails (Coturnix coturnix) infected experimentally 4x104 mg/kg, were divided in seven groups (n=5): A (control-healthy), B (control infected), C (control TW 0.01%), D (NB 0.88 mg/kg), E (drug MTZ 25 mg/kg, F (OE-CL at 0.55 mg/kg) and G (N-CL at 0.44 mg/kg), during 7 consecutive days. RESULTS: The in vitro test showed that the OE-CL (550 µg/ml) and N-CL (440 µg/ml) concentrations reduced the trophozoites viability in 100%. In the in vivo test, the treatment with OE-CL was efficient on the 4th treatment day and the N-CL after the 3rd day, and the MTZ in the therapeutic concentration was efficient on the 7th day. CONCLUSION: It can be observed in this study that the lemon grass has natural potential antitrichomonal activity against T. gallinae in vitro and in vivo.

RESUMO

Background: Trypanosoma evansi is a flagellate which belongs to the salivate section, commonly observed parasitizing blood of equines, ruminants, pigs, dogs and wild animals in different regions of the world. It causes many losses to farmers due to death of animals and drug spending in endemic areas. The treatment of this disease in Brazil is only performed with diminazene aceturate; however it has been ineffective for many animals. During the lats years many studies have been carried out with natural products such as the essential oils. Copaiba oil stands out due some properties described as anti-inflammatory, healing, antiedematogenic, antitumor, parasitic and antibacterial. Therefore, this study aimed to test, in vitro and in vivo, the susceptibility of T. evansi to copaiba oil. Materials, Methods & Results: The oils used in this study were obtained from Copaifera reticulata and Copaifera duckei trees, commonly found in the Tapajos National Forest. The procedure received authorization of IBAMA due the scientific purposes. This study identified three oils identified as copaiba 4-C (C. reticulata), copaiba 5-C (C. duckei) and copaiba 8-C (C. reticulata). The bioassay was performed in vitro using specific culture medium for T. evansi, previously described by Baltz, 1985. Copaiba oil was dissolved in dimethyl sulfoxide (DMSO) and tested in three concentrations (0.5, 1.0 and

Background: Trypanosoma evansi is a flagellate which belongs to the salivate section, commonly observed parasitizing blood of equines, ruminants, pigs, dogs and wild animals in different regions of the world. It causes many losses to farmers due to death of animals and drug spending in endemic areas. The treatment of this disease in Brazil is only performed with diminazene aceturate; however it has been ineffective for many animals. During the lats years many studies have been carried out with natural products such as the essential oils. Copaiba oil stands out due some properties described as anti-inflammatory, healing, antiedematogenic, antitumor, parasitic and antibacterial. Therefore, this study aimed to test, in vitro and in vivo, the susceptibility of T. evansi to copaiba oil. Materials, Methods & Results: The oils used in this study were obtained from Copaifera reticulata and Copaifera duckei trees, commonly found in the Tapajos National Forest. The procedure received authorization of IBAMA due the scientific purposes. This study identified three oils identified as copaiba 4-C (C. reticulata), copaiba 5-C (C. duckei) and copaiba 8-C (C. reticulata). The bioassay was performed in vitro using specific culture medium for T. evansi, previously described by Baltz, 1985. Copaiba oil was dissolved in dimethyl sulfoxide (DMSO) and tested in three concentrations (0.5, 1.0 and

RESUMO

Background: Trypanosoma evansi is a flagellate which belongs to the salivate section, commonly observed parasitizing blood of equines, ruminants, pigs, dogs and wild animals in different regions of the world. It causes many losses to farmers due to death of animals and drug spending in endemic areas. The treatment of this disease in Brazil is only performed with diminazene aceturate; however it has been ineffective for many animals. During the lats years many studies have been carried out with natural products such as the essential oils. Copaiba oil stands out due some properties described as anti-inflammatory, healing, antiedematogenic, antitumor, parasitic and antibacterial. Therefore, this study aimed to test, in vitro and in vivo, the susceptibility of T. evansi to copaiba oil. Materials, Methods & Results: The oils used in this study were obtained from Copaifera reticulata and Copaifera duckei trees, commonly found in the Tapajos National Forest. The procedure received authorization of IBAMA due the scientific purposes. This study identified three oils identified as copaiba 4-C (C. reticulata), copaiba 5-C (C. duckei) and copaiba 8-C (C. reticulata). The bioassay was performed in vitro using specific culture medium for T. evansi, previously described by Baltz, 1985. Copaiba oil was dissolved in dimethyl sulfoxide (DMSO) and tested in three concentrations (0.5, 1.0 and

Background: Trypanosoma evansi is a flagellate which belongs to the salivate section, commonly observed parasitizing blood of equines, ruminants, pigs, dogs and wild animals in different regions of the world. It causes many losses to farmers due to death of animals and drug spending in endemic areas. The treatment of this disease in Brazil is only performed with diminazene aceturate; however it has been ineffective for many animals. During the lats years many studies have been carried out with natural products such as the essential oils. Copaiba oil stands out due some properties described as anti-inflammatory, healing, antiedematogenic, antitumor, parasitic and antibacterial. Therefore, this study aimed to test, in vitro and in vivo, the susceptibility of T. evansi to copaiba oil. Materials, Methods & Results: The oils used in this study were obtained from Copaifera reticulata and Copaifera duckei trees, commonly found in the Tapajos National Forest. The procedure received authorization of IBAMA due the scientific purposes. This study identified three oils identified as copaiba 4-C (C. reticulata), copaiba 5-C (C. duckei) and copaiba 8-C (C. reticulata). The bioassay was performed in vitro using specific culture medium for T. evansi, previously described by Baltz, 1985. Copaiba oil was dissolved in dimethyl sulfoxide (DMSO) and tested in three concentrations (0.5, 1.0 and

RESUMO

Background: The mite Ornithonyssus bursa is a bloodsucking parasite of domestic and wild birds and it is typical of tropical regions. On the host it can cause considerable loss of blood and lesions with intense itching. Cases of parasitism in human beings have been reported causing dermatitis, and most of these cases are due to the presence of nests of synanthropic birds near of the residences. When in large infestations or lack of hosts, the mites leave the nests to perform the parasitism in humans. Human infestation occurs mainly in people who handle infected birds and nests, or when happen the dispersion of these mites, through some path which lead to the interior of homes, factories and hospitals.Case: The present case occurred in November of 2008, when one of the dwellers of an apartment on the fourth floor of a building downtown in Santa Maria, woke up with pruritic lesions over the arms and legs. Upon inspection of the bedroom, several mites were seen, measuring almost a millimeter long, coming out of a pipe of a portable air conditioner installed on site. They were already scattered by the inside walls, floor and bed. The mites were collected and maintained in 70% alcohol for later identification. After the clarification the slides mounting were performed, and the mites with measure between 0.48 mm wide by 0.74 mm long were classified by the position of the anal opening

Background: The mite Ornithonyssus bursa is a bloodsucking parasite of domestic and wild birds and it is typical of tropical regions. On the host it can cause considerable loss of blood and lesions with intense itching. Cases of parasitism in human beings have been reported causing dermatitis, and most of these cases are due to the presence of nests of synanthropic birds near of the residences. When in large infestations or lack of hosts, the mites leave the nests to perform the parasitism in humans. Human infestation occurs mainly in people who handle infected birds and nests, or when happen the dispersion of these mites, through some path which lead to the interior of homes, factories and hospitals.Case: The present case occurred in November of 2008, when one of the dwellers of an apartment on the fourth floor of a building downtown in Santa Maria, woke up with pruritic lesions over the arms and legs. Upon inspection of the bedroom, several mites were seen, measuring almost a millimeter long, coming out of a pipe of a portable air conditioner installed on site. They were already scattered by the inside walls, floor and bed. The mites were collected and maintained in 70% alcohol for later identification. After the clarification the slides mounting were performed, and the mites with measure between 0.48 mm wide by 0.74 mm long were classified by the position of the anal opening

RESUMO

Background: The mite Ornithonyssus bursa is a bloodsucking parasite of domestic and wild birds and it is typical of tropical regions. On the host it can cause considerable loss of blood and lesions with intense itching. Cases of parasitism in human beings have been reported causing dermatitis, and most of these cases are due to the presence of nests of synanthropic birds near of the residences. When in large infestations or lack of hosts, the mites leave the nests to perform the parasitism in humans. Human infestation occurs mainly in people who handle infected birds and nests, or when happen the dispersion of these mites, through some path which lead to the interior of homes, factories and hospitals.Case: The present case occurred in November of 2008, when one of the dwellers of an apartment on the fourth floor of a building downtown in Santa Maria, woke up with pruritic lesions over the arms and legs. Upon inspection of the bedroom, several mites were seen, measuring almost a millimeter long, coming out of a pipe of a portable air conditioner installed on site. They were already scattered by the inside walls, floor and bed. The mites were collected and maintained in 70% alcohol for later identification. After the clarification the slides mounting were performed, and the mites with measure between 0.48 mm wide by 0.74 mm long were classified by the position of the anal opening

Background: The mite Ornithonyssus bursa is a bloodsucking parasite of domestic and wild birds and it is typical of tropical regions. On the host it can cause considerable loss of blood and lesions with intense itching. Cases of parasitism in human beings have been reported causing dermatitis, and most of these cases are due to the presence of nests of synanthropic birds near of the residences. When in large infestations or lack of hosts, the mites leave the nests to perform the parasitism in humans. Human infestation occurs mainly in people who handle infected birds and nests, or when happen the dispersion of these mites, through some path which lead to the interior of homes, factories and hospitals.Case: The present case occurred in November of 2008, when one of the dwellers of an apartment on the fourth floor of a building downtown in Santa Maria, woke up with pruritic lesions over the arms and legs. Upon inspection of the bedroom, several mites were seen, measuring almost a millimeter long, coming out of a pipe of a portable air conditioner installed on site. They were already scattered by the inside walls, floor and bed. The mites were collected and maintained in 70% alcohol for later identification. After the clarification the slides mounting were performed, and the mites with measure between 0.48 mm wide by 0.74 mm long were classified by the position of the anal opening

RESUMO

The objective of this study was to evaluate the lipid peroxidation and the susceptibility of erythrocytes to in vitro peroxidation as indicators of oxidative damage in erythrocytes and their roles in the pathogenesis of anemia during experimental Trypanosoma evansi infection in cats. Animals were divided into two groups: control and infected with T. evansi. Seven cats were infected with 10(8) trypomastigotes each, and parasitemia was estimated daily for 49 days by microscopic examination of smears. Hematological and biochemical parameters were evaluated for monitoring of the disease. Plasma lipid peroxidation (Thiobarbituric Acid Reactive Substances (TBARS)) and the susceptibility of erythrocytes to in vitro peroxidation were evaluated. Blood samples for analysis were collected at days 21 and 49 post-inoculation. TBARS level, indicated by MDA concentration, was higher in the infected group than in the control group in both analyzed periods, as well as the in vitro erythrocyte peroxidation (P < 0.001). The infected cats had variable degrees of regenerative anemia, which could be explained by the damage in erythrocyte membrane caused by lipid peroxidation.

Assuntos

RESUMO

Este trabalho teve o objetivo de avaliar a eficácia de antiparasitários no controle de helmintoses em avestruzes (Struthiocamelus) naturalmente infectados por helmintos da ordem Strongylida em criatório comercial no município de Restinga Sêca,estado do Rio Grande do Sul, Brasil. Neste estudo foram utilizados quinze animais adultos, divididos em três grupos, grupo A,utilizado como controle, grupo B animais tratados com levamisole (5mg Kg-1) e o grupo C, aves tratadas com fenbendazole (5mgKg-1). Para avaliar a eficácia do tratamento foram realizadas duas coletas de fezes, a primeira no dia zero, antes do tratamento ea segunda coleta dez dias após a medicação, sendo processadas pela técnica de centrífugo-flutuação com sulfato de zinco. No diazero, todos os animais apresentaram no exame de fezes elevada infecção por ovos de helmintos, já no dia 10 os animais do grupoC estavam negativos, ao contrário dos demais grupos que mantiveram a infecção. Com base nos resultados, conclui-se que ofenbendazole na dose utilizada foi eficaz no tratamento de avestruzes infectadas com nematódeos da ordem Strongylida.

RESUMO

Este trabalho teve o objetivo de avaliar a eficácia de antiparasitários no controle de helmintoses em avestruzes (Struthiocamelus) naturalmente infectados por helmintos da ordem Strongylida em criatório comercial no município de Restinga Sêca,estado do Rio Grande do Sul, Brasil. Neste estudo foram utilizados quinze animais adultos, divididos em três grupos, grupo A,utilizado como controle, grupo B animais tratados com levamisole (5mg Kg-1) e o grupo C, aves tratadas com fenbendazole (5mgKg-1). Para avaliar a eficácia do tratamento foram realizadas duas coletas de fezes, a primeira no dia zero, antes do tratamento ea segunda coleta dez dias após a medicação, sendo processadas pela técnica de centrífugo-flutuação com sulfato de zinco. No diazero, todos os animais apresentaram no exame de fezes elevada infecção por ovos de helmintos, já no dia 10 os animais do grupoC estavam negativos, ao contrário dos demais grupos que mantiveram a infecção. Com base nos resultados, conclui-se que ofenbendazole na dose utilizada foi eficaz no tratamento de avestruzes infectadas com nematódeos da ordem Strongylida.

RESUMO

Tupinambis teguixin é o maior lagarto presente no Brasil. Neste estudo foram analisados dois exemplares encontrados mortos no município de Alegria, estado do Rio Grande do Sul, Brasil. Nestes animais observaram-se nematóides dos gêneros Physaloptera e Kalicephalus e protozoários do gênero Cryptosporidium. O presente trabalho registra o primeiro caso de parasitismo por estes gêneros de parasitas em lagarto T. teguixin de vida livre no país.

RESUMO

This study aimed to evaluate the efficacy of metronidazole, fenbendazole and secnidazole against Giardia muris in mice naturally infected. Forty mice of the species Mus musculus were divided in four groups of ten each, being group A non treated, the control group and groups B, C and D treated with 4mg/ml of metronidazole, fenbendazole and secnidazole, respectively. Two feces collection, on day 0 and on day 10 after treatment, were done in order to evaluate the efficacy of the drugs. Samples were analyzed by the centrifugal-flotation method with zinc sulfate. Efficacy of 97,05% for metronidazole, 98,30% for fenbendazole and 100% for secnidazole were observed in the study. According to the results it was concluded that the tested drugs were effective for the treatment of mice parasitized by Giardia muris.

Este estudo visou avaliar a eficácia do metronidazol, fenbendazole e secnidazol contra Giardia muris em camundongos naturalmente infectados. Foram utilizados 40 camundongos da espécie Mus musculus divididos em quatro grupos de 10 animais cada, sendo grupo A, grupo controle, não tratados, e grupos B, C e D tratados com 4mg/ml de metronidazol, fenbendazole e secnidazol, respectivamente. Para avaliar a eficácia dos medicamentos foram realizadas duas coletas de fezes uma no dia zero e outra 10 dias após tratamento. As amostras foram processadas e analisadas a partir do método de centrífugo-flutuação com sulfato de zinco. No estudo observou-se eficácia de 97,05% para metronidazol, 98,30% para fenbendazole e 100% para secnidazol no tratamento de giardiase murina. Com base nos resultados concluí-se que as drogas testadas apresentaram eficácia no tratamento de camundongos parasitados por Giardia muris.

RESUMO

The objective of this work is reporting the parasitism in agouti (Dasyprocta leporine) in Rio Grande do Sul, for that 24 samples of feces of animals originating from of a commercial creation were collected. The samples analyzed by the centrifugal-floating method presented different nematodes eggs and protozoaires of the genus Giardia and Eimeria. Keywords: Dasyprocta, parasitism, Giardia, Eimeria, helmints

O objetivo deste trabalho é relatar o parasitismo em cutias (Dasyprocta leporina) no Rio Grande do Sul, para isso foram coletadas 24 amostras de fezes de animais oriundos de uma criação comercial. As amostras analisadas pelo método de centrífugo-flutuação apresentaram diferentes ovos de nematóides e protozoários do gênero Giardia e Eimeria. Palavras - chave: Dasyprocta, parasitismo, Giardia, Eimeria, helmintos.

RESUMO

Tupinambis teguixin é o maior lagarto presente no Brasil. Neste estudo foram analisados dois exemplares encontrados mortos no município de Alegria, estado do Rio Grande do Sul, Brasil. Nestes animais observaram-se nematóides dos gêneros Physaloptera e Kalicephalus e protozoários do gênero Cryptosporidium. O presente trabalho registra o primeiro caso de parasitismo por estes gêneros de parasitas em lagarto T. teguixin de vida livre no país.

RESUMO

The objective of this work is reporting the parasitism in agouti (Dasyprocta leporine) in Rio Grande do Sul, for that 24 samples of feces of animals originating from of a commercial creation were collected. The samples analyzed by the centrifugal-floating method presented different nematodes eggs and protozoaires of the genus Giardia and Eimeria. Keywords: Dasyprocta, parasitism, Giardia, Eimeria, helmints

O objetivo deste trabalho é relatar o parasitismo em cutias (Dasyprocta leporina) no Rio Grande do Sul, para isso foram coletadas 24 amostras de fezes de animais oriundos de uma criação comercial. As amostras analisadas pelo método de centrífugo-flutuação apresentaram diferentes ovos de nematóides e protozoários do gênero Giardia e Eimeria. Palavras - chave: Dasyprocta, parasitismo, Giardia, Eimeria, helmintos.

RESUMO

Trypanosoma evansi  é um protozoário de distribuição geográfica mundial, detectado na corrente sanguínea de várias espécies de mamíferos. O presente estudo visou relatar um caso de tripanossomose por T. evansi em bovinos de uma propriedade leiteira no município de Videira, Santa Catarina. Nos meses de maio a junho de 2007, dez bovinos adoeceram apresentando sinais clínicos de anorexia, apatia, emagrecimento progressivo, fraqueza, hipertermia (41ºC), mucosas pálidas e hemorragias cutâneas após a morte. Os animais foram tratados para anaplasmose e babesiose, no entanto, quatro não responderam ao tratamento e morreram. Em uma novilha que morreu foi realizado esfregaço sanguíneo periférico. Baseado nos sinais clínicos e nos achados de esfregaço sanguíneo diagnosticou-se o caso como sendo uma infecção mista por T. evansi e Anaplasma marginale, sendo este o primeiro caso deste protozoário em bovino na região sul do país. O desconhecimento da tripanossomose na região sul dificulta o diagnóstico da enfermidade, pois esta apresenta evolução semelhante a outras hemoparasitoses.

RESUMO

Trypanosoma evansi  é um protozoário de distribuição geográfica mundial, detectado na corrente sanguínea de várias espécies de mamíferos. O presente estudo visou relatar um caso de tripanossomose por T. evansi em bovinos de uma propriedade leiteira no município de Videira, Santa Catarina. Nos meses de maio a junho de 2007, dez bovinos adoeceram apresentando sinais clínicos de anorexia, apatia, emagrecimento progressivo, fraqueza, hipertermia (41ºC), mucosas pálidas e hemorragias cutâneas após a morte. Os animais foram tratados para anaplasmose e babesiose, no entanto, quatro não responderam ao tratamento e morreram. Em uma novilha que morreu foi realizado esfregaço sanguíneo periférico. Baseado nos sinais clínicos e nos achados de esfregaço sanguíneo diagnosticou-se o caso como sendo uma infecção mista por T. evansi e Anaplasma marginale, sendo este o primeiro caso deste protozoário em bovino na região sul do país. O desconhecimento da tripanossomose na região sul dificulta o diagnóstico da enfermidade, pois esta apresenta evolução semelhante a outras hemoparasitoses.

RESUMO

This study was aimed at reporting the presence of Temnocephala sp. in fresh water turtle (Hydromedusa tectifera) central area of Rio Grande do Sul, Brazil. The platyhelminths have been found fastened in body, members, and shell of the host being collected through cutaneous scratching. In the laboratory they were assembled between glass sheets and identified as being belonging to the Temnocephala genus.

O objetivo deste trabalho foi relatar a presença de Temnocephala sp. em tartaruga de água doce (Hydromedusa tectifera) na região central do Rio Grande do Sul, Brasil. Os platelmintos foram encontrados fixados no corpo, membros e casco do hospedeiro, sendo coletados através de raspagem cutânea. No laboratório, foram montados entre lâminas de vidro e identificados como pertencentes ao gênero Temnocephala.

RESUMO

In this research, Trypanosoma evansi infection was tested in rats and mice by oral ingestion of contaminated blood. Groups of ten rats and ten mice were disposed in four experimental groups: A and B (rats), C and D (mice). The groups A and C were contaminated by rat-contaminated blood; B and C groups by mouse-contaminated blood. The blood was given using a probe filled with 0.2ml of contaminated blood with 10(7) trypanosomes ml-1. These animals were maintained at constant temperature and humidity (25°C and 80% UR). Dairy blood smear were done to identify the prepatent period and evolution of parasite in the circulation. In the A and B groups, the pre latency period varied from 19 to 25 days and the period of parasite detection and animals death was an average of 12.7 days. The C and D groups did not present infection by the parasite even when evaluated for 60 days. In conclusion, the rats had oral infection by T. evansi but this protozoan couldnt contaminate the mice by digestive path.

Testou-se a infecção de Trypanosoma evansi pela via oral em ratos e camundongos, através de sangue contaminado de ambas as espécies. Dez ratos e dez camundongos foram alocados em quatro grupos iguais A e B (ratos), C e D (camundongos). Os grupos A e C receberam sangue contaminado de um rato e o grupo B e D de um camundongo, através de uma sonda. O volume de sangue administrado foi de 0,2ml, o qual apresentava uma concentração de 10(7) tripanossomas ml-1. Os animais foram mantidos em temperatura e umidade constantes (25°C e 80% UR), sendo realizados esfregaços sanguíneos diários para identificar o período pré-patente e a evolução do parasita na circulação. Nos grupos A e B, o período pré-patente variou de 19 a 25 dias, e o período entre a detecção dos parasitas e a morte dos animais foi em média de 12,7 dias. Os camundongos do grupo C e D não apresentaram infecção pelo parasita, sendo estes avaliados por 60 dias. Os ratos foram susceptíveis a infecção por T. evansi pela via oral; entretanto, os camundongos não se contaminaram com o protozoário por via digestiva.

RESUMO

In this research, Trypanosoma evansi infection was tested in rats and mice by oral ingestion of contaminated blood. Groups of ten rats and ten mice were disposed in four experimental groups: A and B (rats), C and D (mice). The groups A and C were contaminated by rat-contaminated blood; B and C groups by mouse-contaminated blood. The blood was given using a probe filled with 0.2ml of contaminated blood with 10(7) trypanosomes ml-1. These animals were maintained at constant temperature and humidity (25°C and 80% UR). Dairy blood smear were done to identify the prepatent period and evolution of parasite in the circulation. In the A and B groups, the pre latency period varied from 19 to 25 days and the period of parasite detection and animals death was an average of 12.7 days. The C and D groups did not present infection by the parasite even when evaluated for 60 days. In conclusion, the rats had oral infection by T. evansi but this protozoan couldn’t contaminate the mice by digestive path.

Testou-se a infecção de Trypanosoma evansi pela via oral em ratos e camundongos, através de sangue contaminado de ambas as espécies. Dez ratos e dez camundongos foram alocados em quatro grupos iguais A e B (ratos), C e D (camundongos). Os grupos A e C receberam sangue contaminado de um rato e o grupo B e D de um camundongo, através de uma sonda. O volume de sangue administrado foi de 0,2ml, o qual apresentava uma concentração de 10(7) tripanossomas ml-1. Os animais foram mantidos em temperatura e umidade constantes (25°C e 80% UR), sendo realizados esfregaços sanguíneos diários para identificar o período pré-patente e a evolução do parasita na circulação. Nos grupos A e B, o período pré-patente variou de 19 a 25 dias, e o período entre a detecção dos parasitas e a morte dos animais foi em média de 12,7 dias. Os camundongos do grupo C e D não apresentaram infecção pelo parasita, sendo estes avaliados por 60 dias. Os ratos foram susceptíveis a infecção por T. evansi pela via oral; entretanto, os camundongos não se contaminaram com o protozoário por via digestiva.

RESUMO

This study was aimed at reporting the presence of Temnocephala sp. in fresh water turtle (Hydromedusa tectifera) central area of Rio Grande do Sul, Brazil. The platyhelminths have been found fastened in body, members, and shell of the host being collected through cutaneous scratching. In the laboratory they were assembled between glass sheets and identified as being belonging to the Temnocephala genus.

O objetivo deste trabalho foi relatar a presença de Temnocephala sp. em tartaruga de água doce (Hydromedusa tectifera) na região central do Rio Grande do Sul, Brasil. Os platelmintos foram encontrados fixados no corpo, membros e casco do hospedeiro, sendo coletados através de raspagem cutânea. No laboratório, foram montados entre lâminas de vidro e identificados como pertencentes ao gênero Temnocephala.