RESUMO
PIP: The study investigates hormonal control of the menstrual cycle, adrenal function role, and endouterine menstrual disorders by an analysis of peripheral hormones and of their concentration at endometrial level. The authors also present a new radioimmunoassay method for determining hormone presence in plasma and in the endometrium. 59 ovulatory cycles were considered, and ovarian hormones were found to be 10-20 times higher in tissue than in plasma, but not in women under sequential or combined oral contraceptive treatment. The relation between endometrial estrogens and progesterone with plasma follicle stimulating hormone and luteinizing hormone levels is discussed, together with the significance of tissue hormone concentration.^ieng
Assuntos
Anticoncepcionais Orais Hormonais/farmacologia , Anticoncepcionais Orais/farmacologia , Endométrio/análise , Hormônio Foliculoestimulante/análise , Hormônio Luteinizante/análise , Ovário/análise , Cromatografia/métodos , Endométrio/efeitos dos fármacos , Feminino , Humanos , Menstruação/efeitos dos fármacos , Gravidez , Radioimunoensaio/métodosRESUMO
A radioimmunoassay procedure for testosterone (T) and dihydrotestosterone (DHT) in blood and semen plasma was developed using antisera against both androgens. The method utilized 0.5 ml and 1.0 ml volumes of blood plasma and semen respectively, and satisfies the recognized criteria of reliability. Determinations of T and DHT were performed on 33 healthy male unmarried subjects, aged 21-25 years old, and then compared to the sperm count. The T/DHT blood ratio was 7.8 and the correlation was r = 0.50, P less than 0.01, while the T/DHT semen ratio was 0.42 and the correlation r = 0.55, P less than 0.01. The T/DHT, blood/semen ratio was 10.8. No significant relationship was observed either between blood plasma androgens or semen plasma androgens and sperm concentrations.
Assuntos
Androgênios/análise , Sêmen/análise , Adulto , Androgênios/sangue , Humanos , Infertilidade Masculina/diagnóstico , Masculino , Pessoa Solteira , Contagem de EspermatozoidesRESUMO
In 65 patients with menstrual cycle alterations--in it's length and/or amount of bleeding--estradiol-progesterone relationship was studied. The plasma levels of these hormones were correlated with basal temperature, length of menstrual cycle and histologic study of endometrium. The patient- were grouped as follows: I excessive menstrual bleeding; II short menstrual cycles; III long menstrual cycles; IV increment of bleeding period and, V uterine hemorrhage. All patients had an anovulatory menstrual cycle; peripheral concentration of progeseterone was less than 6 ng/ml and mean concentration of estradiol was not statistically higher than that found in normal patients (83+/-23 pg/ml). Based upon these findings, it is postulated that the term of hyperestrogenism or excessive estrogen production should not be used when referring to these menstrual cycle alterations.